rif  (Valiant)

 
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  • 93
    Name:
    Rifampin
    Description:
    Rifampin
    Catalog Number:
    0219479581
    Price:
    45.15
    Category:
    Life Sciences Biochemicals Antibiotics
    Applications:
    Antibiotic, Inhibitor of transcription initiation, Antipruritic agent
    Size:
    150 mg
    Buy from Supplier


    Structured Review

    Valiant rif
    Flavin reduction with <t>NADPH.</t> Spectra changes for the substrate-free RifMO with 2 mM NADPH. (B) Change in the flavin absorbance at 450 nm for substrate-free RifMO at various concentrations of NADPH (0.025–2 mM). (C) Dependence of the k obs values as a function of NADPH in the presence of 15 μM <t>Rif.</t> Data was fit to a single exponential decay equation. (D) Spectra changes for the Rif-RifMO complex with 2 mM NADPH. (E) Change in the flavin absorbance at 450 nm for substrate-complexed RifMO at various concentrations of NADPH (0.025–5 mM). (F) Dependence of the k obs values as a function of NADPH in the absence of Rif. Data was fit to a single exponential decay equation.
    Rifampin
    https://www.bioz.com/result/rif/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rif - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Mechanism of Rifampicin Inactivation in Nocardia farcinica"

    Article Title: Mechanism of Rifampicin Inactivation in Nocardia farcinica

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0162578

    Flavin reduction with NADPH. Spectra changes for the substrate-free RifMO with 2 mM NADPH. (B) Change in the flavin absorbance at 450 nm for substrate-free RifMO at various concentrations of NADPH (0.025–2 mM). (C) Dependence of the k obs values as a function of NADPH in the presence of 15 μM Rif. Data was fit to a single exponential decay equation. (D) Spectra changes for the Rif-RifMO complex with 2 mM NADPH. (E) Change in the flavin absorbance at 450 nm for substrate-complexed RifMO at various concentrations of NADPH (0.025–5 mM). (F) Dependence of the k obs values as a function of NADPH in the absence of Rif. Data was fit to a single exponential decay equation.
    Figure Legend Snippet: Flavin reduction with NADPH. Spectra changes for the substrate-free RifMO with 2 mM NADPH. (B) Change in the flavin absorbance at 450 nm for substrate-free RifMO at various concentrations of NADPH (0.025–2 mM). (C) Dependence of the k obs values as a function of NADPH in the presence of 15 μM Rif. Data was fit to a single exponential decay equation. (D) Spectra changes for the Rif-RifMO complex with 2 mM NADPH. (E) Change in the flavin absorbance at 450 nm for substrate-complexed RifMO at various concentrations of NADPH (0.025–5 mM). (F) Dependence of the k obs values as a function of NADPH in the absence of Rif. Data was fit to a single exponential decay equation.

    Techniques Used:

    Steady-state kinetics of oxygen consumption compared to HPLC analysis. (A) Reaction rates as a function of NADPH using oxygraph (solid circles) and HPLC (open circles). The inset shows the oxygen consumption activity at higher NADPH concentration. (B) Reaction rates as a function of Rif using oxygraph (open circles) and HPLC (closed circles) in the presence of 1 mM NADPH as the electron donor. Oxygen consumption assays were done in 1 mL of 100 mM sodium phosphate, pH 7.5, at 25°C. (C) Oxygen consumption as a function of NADH. (D) Oxygen consumption as a function of Rif in the presence of 2 mM NADH as the electron donor.
    Figure Legend Snippet: Steady-state kinetics of oxygen consumption compared to HPLC analysis. (A) Reaction rates as a function of NADPH using oxygraph (solid circles) and HPLC (open circles). The inset shows the oxygen consumption activity at higher NADPH concentration. (B) Reaction rates as a function of Rif using oxygraph (open circles) and HPLC (closed circles) in the presence of 1 mM NADPH as the electron donor. Oxygen consumption assays were done in 1 mL of 100 mM sodium phosphate, pH 7.5, at 25°C. (C) Oxygen consumption as a function of NADH. (D) Oxygen consumption as a function of Rif in the presence of 2 mM NADH as the electron donor.

    Techniques Used: High Performance Liquid Chromatography, Activity Assay, Concentration Assay

    Time-dependent HPLC analysis of RifMO reactions. (A) Stacked chromatograms showing time traces for the elution of the Rif peak (21.2 min), P* (13.4 min), Rif-OH (22.1 min), and the P* degradation compound (6.7 min). (B) Stacked chromatograms show P* (A) extracted in 100 mM sodium phosphate buffer, pH 7.5, incubated with: (B) NADPH, (C) RifMO, (D, E) NADPH and RifMO, for 5, and 20 min., respectively.
    Figure Legend Snippet: Time-dependent HPLC analysis of RifMO reactions. (A) Stacked chromatograms showing time traces for the elution of the Rif peak (21.2 min), P* (13.4 min), Rif-OH (22.1 min), and the P* degradation compound (6.7 min). (B) Stacked chromatograms show P* (A) extracted in 100 mM sodium phosphate buffer, pH 7.5, incubated with: (B) NADPH, (C) RifMO, (D, E) NADPH and RifMO, for 5, and 20 min., respectively.

    Techniques Used: High Performance Liquid Chromatography, Incubation

    Related Articles

    Concentration Assay:

    Article Title: Unveiling the physiological mechanisms that drive the emergence of antibiotic resisters from antibiotic persister population of mycobacteria
    Article Snippet: .. Growth curve experiments From the starting of the experiment, liquid cultures were exposed to 25 µg/ml concentration of the antibiotic rifampin (MP Biomedicals). .. The antibiotic stock solution (50 mg/ml) was made by dissolving rifampin powder (MP Biomedicals) in DMSO (Merck Millipore) and filter sterilised using 0.22 µm PVDF syringe filters (Millex-GV).

    Article Title: Unique Mode of Cell Division by the Mycobacterial Genetic Resister Clones Emerging De Novo from the Antibiotic-Surviving Population
    Article Snippet: .. From the start of the experiment, liquid cultures were exposed to 25 μg/ml (10× MBC)—2.5 μg/ml (1× MBC) concentration of the antibiotic, rifampicin (MP Biomedicals). .. The antibiotic stock solution (50 mg/ml) was made by dissolving rifampicin powder (MP Biomedicals) in dimethyl sulfoxide (DMSO; Merck Millipore) and was filter sterilized using 0.22-μm polyvinylidene difluoride (PVDF) syringe filters (Millex-GV).

    other:

    Article Title: Vancomycin Tolerant, Methicillin-Resistant Staphylococcus aureus Reveals the Effects of Vancomycin on Cell Wall Thickening
    Article Snippet: MIC tests were performed for ampicillin (Sigma-Aldrich), ceftazidime (Pfizer; México DF, México), ceftriaxone (Roche; Mexico DF, México), ciprofloxacin (Bayer; Levenkusen Westfalia, Germany), erythromycin (MP Biomedicals; Solon, OH, USA), kanamycin (Sigma-Aldrich), meropenem (Astra Zeneca; México DF, Mexico), rifampicin (MP Biomedicals), gentamicin (MP Biomedicals), and trimethoprim-sulfamethoxazole (Sigma-Aldrich).

    Article Title: Unique Mode of Cell Division by the Mycobacterial Genetic Resister Clones Emerging De Novo from the Antibiotic-Surviving Population
    Article Snippet: Plating experiments were performed on Mycobacterium 7H11 agar plates (Difco), with rifampicin (125 μg/ml; MP Biomedicals) or without rifampicin, at 37°C for 3 to 4 days.

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  • rif  (Valiant)
    93
    Valiant rif
    Flavin reduction with <t>NADPH.</t> Spectra changes for the substrate-free RifMO with 2 mM NADPH. (B) Change in the flavin absorbance at 450 nm for substrate-free RifMO at various concentrations of NADPH (0.025–2 mM). (C) Dependence of the k obs values as a function of NADPH in the presence of 15 μM <t>Rif.</t> Data was fit to a single exponential decay equation. (D) Spectra changes for the Rif-RifMO complex with 2 mM NADPH. (E) Change in the flavin absorbance at 450 nm for substrate-complexed RifMO at various concentrations of NADPH (0.025–5 mM). (F) Dependence of the k obs values as a function of NADPH in the absence of Rif. Data was fit to a single exponential decay equation.
    Rif, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rif/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rif - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Flavin reduction with NADPH. Spectra changes for the substrate-free RifMO with 2 mM NADPH. (B) Change in the flavin absorbance at 450 nm for substrate-free RifMO at various concentrations of NADPH (0.025–2 mM). (C) Dependence of the k obs values as a function of NADPH in the presence of 15 μM Rif. Data was fit to a single exponential decay equation. (D) Spectra changes for the Rif-RifMO complex with 2 mM NADPH. (E) Change in the flavin absorbance at 450 nm for substrate-complexed RifMO at various concentrations of NADPH (0.025–5 mM). (F) Dependence of the k obs values as a function of NADPH in the absence of Rif. Data was fit to a single exponential decay equation.

    Journal: PLoS ONE

    Article Title: Mechanism of Rifampicin Inactivation in Nocardia farcinica

    doi: 10.1371/journal.pone.0162578

    Figure Lengend Snippet: Flavin reduction with NADPH. Spectra changes for the substrate-free RifMO with 2 mM NADPH. (B) Change in the flavin absorbance at 450 nm for substrate-free RifMO at various concentrations of NADPH (0.025–2 mM). (C) Dependence of the k obs values as a function of NADPH in the presence of 15 μM Rif. Data was fit to a single exponential decay equation. (D) Spectra changes for the Rif-RifMO complex with 2 mM NADPH. (E) Change in the flavin absorbance at 450 nm for substrate-complexed RifMO at various concentrations of NADPH (0.025–5 mM). (F) Dependence of the k obs values as a function of NADPH in the absence of Rif. Data was fit to a single exponential decay equation.

    Article Snippet: Materials NADPH and Rif were purchased from MP Biomedicals (Billerica, MA).

    Techniques:

    Steady-state kinetics of oxygen consumption compared to HPLC analysis. (A) Reaction rates as a function of NADPH using oxygraph (solid circles) and HPLC (open circles). The inset shows the oxygen consumption activity at higher NADPH concentration. (B) Reaction rates as a function of Rif using oxygraph (open circles) and HPLC (closed circles) in the presence of 1 mM NADPH as the electron donor. Oxygen consumption assays were done in 1 mL of 100 mM sodium phosphate, pH 7.5, at 25°C. (C) Oxygen consumption as a function of NADH. (D) Oxygen consumption as a function of Rif in the presence of 2 mM NADH as the electron donor.

    Journal: PLoS ONE

    Article Title: Mechanism of Rifampicin Inactivation in Nocardia farcinica

    doi: 10.1371/journal.pone.0162578

    Figure Lengend Snippet: Steady-state kinetics of oxygen consumption compared to HPLC analysis. (A) Reaction rates as a function of NADPH using oxygraph (solid circles) and HPLC (open circles). The inset shows the oxygen consumption activity at higher NADPH concentration. (B) Reaction rates as a function of Rif using oxygraph (open circles) and HPLC (closed circles) in the presence of 1 mM NADPH as the electron donor. Oxygen consumption assays were done in 1 mL of 100 mM sodium phosphate, pH 7.5, at 25°C. (C) Oxygen consumption as a function of NADH. (D) Oxygen consumption as a function of Rif in the presence of 2 mM NADH as the electron donor.

    Article Snippet: Materials NADPH and Rif were purchased from MP Biomedicals (Billerica, MA).

    Techniques: High Performance Liquid Chromatography, Activity Assay, Concentration Assay

    Time-dependent HPLC analysis of RifMO reactions. (A) Stacked chromatograms showing time traces for the elution of the Rif peak (21.2 min), P* (13.4 min), Rif-OH (22.1 min), and the P* degradation compound (6.7 min). (B) Stacked chromatograms show P* (A) extracted in 100 mM sodium phosphate buffer, pH 7.5, incubated with: (B) NADPH, (C) RifMO, (D, E) NADPH and RifMO, for 5, and 20 min., respectively.

    Journal: PLoS ONE

    Article Title: Mechanism of Rifampicin Inactivation in Nocardia farcinica

    doi: 10.1371/journal.pone.0162578

    Figure Lengend Snippet: Time-dependent HPLC analysis of RifMO reactions. (A) Stacked chromatograms showing time traces for the elution of the Rif peak (21.2 min), P* (13.4 min), Rif-OH (22.1 min), and the P* degradation compound (6.7 min). (B) Stacked chromatograms show P* (A) extracted in 100 mM sodium phosphate buffer, pH 7.5, incubated with: (B) NADPH, (C) RifMO, (D, E) NADPH and RifMO, for 5, and 20 min., respectively.

    Article Snippet: Materials NADPH and Rif were purchased from MP Biomedicals (Billerica, MA).

    Techniques: High Performance Liquid Chromatography, Incubation