streptomycin (Valiant)

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Name:

Streptomycin sulfate cell culture reagent

Description:

Streptomycin Sulfate is an aminoglucoside antibiotic in which streptidine is linked glycosidically to the disaccharide streptobiosamine It is most active in a slightly alkaline medium Inhibits initiation and causes misreading of rRNA in protein synthesis Effective against gram negative and gram positive bacteria About one third of streptomycin in the circulation is bound to plasma proteins About 30 90 of a dose is excreted unchanged in the urine in 24 hours

Catalog Number:

0219454105

Price:

20.0

Category:

Life Sciences Biochemicals Antibiotics

Applications:

Antibiotic, Pesticide, Cell culture, Treatment of Tuberculosis, Plague

Size:

5 g

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Structured Review

Valiant streptomycin
Streptomycin Sulfate is an aminoglucoside antibiotic in which streptidine is linked glycosidically to the disaccharide streptobiosamine It is most active in a slightly alkaline medium Inhibits initiation and causes misreading of rRNA in protein synthesis Effective against gram negative and gram positive bacteria About one third of streptomycin in the circulation is bound to plasma proteins About 30 90 of a dose is excreted unchanged in the urine in 24 hours
https://www.bioz.com/result/streptomycin/product/Valiant
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

streptomycin - by Bioz Stars, 2021-03

93/100 stars

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In Vitro:

Article Title: BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin
Article Snippet: Silencing of STAT1 by siRNA as well as inhibition of the activation of AKT by inhibitor led to a decrease in the expression of BST2 and E-cadherin, thereby reversing the observed decreased invasion/spreading of HTR-8/SVneo cells by IFN-γ, suggesting their relevance in IFN-γ-mediated invasion of trophoblast cells. .. In vitro culture of HTR-8/SVneo cells and Ishikawa cells HTR-8/SVneo cells derived from human first-trimester placental explant cultures and immortalized by Simian virus (SV40) large T antigen [ ] were cultured in 1:1 ratio of Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich Inc., St. Louis, MO, USA; #D1152-10L) and Ham’s F-12 medium (Sigma-Aldrich Inc., #N3520-10L) supplemented with 10% fetal bovine serum (FBS; Gibco® Life Technologies, Grand Island, NY, USA; #10270) and antibiotic-antimycotic cocktail [streptomycin (100 units/mL), penicillin (100 µg/mL), and amphotericin B (0.25 µg/mL; MP Biomedicals, Santa Ana, CA, USA; #1674049)] at 37°C under 5% CO2 and 70% relative humidity. .. Ishikawa endometrial epithelial cells were also cultured as described for HTR-8/SVneo cells.

Derivative Assay:

Cell Culture:

Article Title: Hydrostatic pressure can induce apoptosis of the skin
Article Snippet: .. Inducing apoptosis of human dermal fibroblasts Human dermal fibroblast adult (hDFa; Catalog number: C0135C; Thermo Fisher Scientific Inc., Waltham, MA, USA) cells were cultured using Dulbecco’s modified Eagle’s medium (DMEM; “Nissui”1; Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA) and 1% antibiotic/penicillin and streptomycin solution (MP Biomedicals, LLC, Solon, OH, USA) at 37 °C, 95% humidity, and 5% CO2 . .. After rapidly thawing in a water bath, 1 × 106 cells were seeded and cultured on a 10- or 15-cm culture dish (Falcon; Corning Inc., Corning, NY, USA), after which the medium was changed every 1 or 2 days.

Article Title: Bauhinia purprea agglutinin‐modified liposomes for human prostate cancer treatment
Article Snippet: .. Cell culture Human prostate cancer cells lines DU145 and LNCaP were cultured in RPMI‐1640 medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% heat‐inactivated FBS (AusGeneX Pty Ltd, Brisbane, Australia), 100 μg/mL streptomycin (MP Biomedicals, Irvine, CA, USA) and 100 units/mL penicillin (MP Biomedicals) at 37°C in a humidified atmosphere with 5% CO2 . .. Histological analysis A deparaffinized tissue array slide (human normal prostate and cancer tissue; SuperBioChips Laboratories, Seoul, Korea) was blocked with 1% BSA‐PBS for 30 min. Then, biotin‐conjugated BPA (50 μg/mL) was added to the array slide; and incubation was carried out for 1 h at room temperature.

Article Title: Hyperthermia enhances 17-DMAG efficacy in hepatocellular carcinoma cells with aggravated DNA damage and impaired G2/M transition
Article Snippet: Cell culture Hepatic cell line L02 , hepatocellular carcinoma (HCC) cell lines Huh7 and HepG2 , hepatic cell line QGY-7703 and HCC cell line QSG-7701 which were isolated from the same hepatocellular carcinoma patient , were purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. .. All cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco) containing 10% fetal bovine serum (Gibco), 100 μg/mL streptomycin sodium and 100 U/mL penicillin G sodium (MP Biomedicals) at 37 °C and 5% CO2 . .. Cell treatment 17-DMAG (Selleck) was added into the medium before cells were transferred to another incubator, of which the temperature had been set to 42 °C, for the indicated time plus 15 min.

Article Title: Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure
Article Snippet: Cell Lines and Culture Condition We purchased and prepared five types of cells: human dermal fibroblasts, adult (HDFas; Catalog number: C0135C; Life Technologies Co., Ltd., Carlsbad, CA, USA), adipose-derived stem cells (ASCs; Lot number: ASC0044; DS Pharma Biomedical Co., Ltd., Osaka, Japan), human epidermal keratinocytes, adult (HEKas; Catalog number: C0055C; Life Technologies Co., Ltd.), human epidermal melanocytes, adult (HEMa-LPs; Catalog number: C0245C, Life Technologies Co., Ltd.), and malignant melanoma cells (MMs; Public Health England Culture Collection, Porton Down, UK). .. HDFas, ASCs, and MMs were cultured using Dulbecco's modified Eagle's medium (DMEM; “Nissui”1; Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) containing 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA) and 1% antibiotic/penicillin and streptomycin solution (MP Biomedicals, LLC, Solon, OH, USA) at 37°C, 95% humidity, and 5% carbon dioxide. .. HEMa-LPs were cultured in M-254 medium (Life Technologies Co., Ltd.) supplemented with PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2, Life Technologies Co., Ltd.) at 37°C, 95% humidity, and 5% carbon dioxide.

Article Title: Interactions Between the Circadian Clock and Heme Oxygenase in the Retina of Drosophila melanogaster
Article Snippet: .. Cells were cultured in Schneider’s medium (Invitrogen) with 10 % fetal bovine serum (Invitrogen) and 100 U/ml penicillin/100 μg/ml streptomycin (MP Biomedicals) at 25 °C. .. Experimental Procedure To detect cyclic expression of ho in the retina, we analyzed ho messenger RNA (mRNA) level at five time points in LD12:12 and DD conditions: 1 h after the beginning of the day (Zeitgeber Time, ZT1)/the subjective day (Circadian Time, CT1); 4 h after the beginning of the day (ZT4)/the subjective day (CT4); 1 h after the beginning of the night (ZT13)/the subjective night (CT13); 4 h after the beginning of the night (ZT16)/the subjective night (CT16); and 8 h after the beginning of the night (ZT20)/the subjective night (CT20).

Modification:

Staining:

Article Title: Apolipoprotein A-I gene transfer exerts immunomodulatory effects and reduces vascular inflammation and fibrosis in ob/ob mice
Article Snippet: Cell culture Murine C4 fibroblasts, a murine fibroblast cell line derived from embryonic BALB/c mice by SV40 infection in vitro [ ], were cultured in Basal Iscove medium supplemented with 10 % FBS and 1 % Penicillin/Streptomycin. .. Twenty-four h after plating at a cell density of 10,000 cells/96-well, cells were stimulated with/out 10 ng/ml TGF-ß1 (R & D Systems, Minneapolis, USA) under serum starvation conditions, i.e. Basal Iscove medium with 0.01 % FBS and 1 % Pencillin/Streptomycin, in the presence or absence of 50 μg/ml of HDL (MP Biomedicals, Solon, Ohio, USA) for 24 h. Next, cells were fixed in methanol overnight at −20 °C for subsequent collagen deposition staining. .. Human aortic endothelial cells (HAEC; Lonza, Basel, Switzerland) were cultured in Endothelial Basal Medium (Lonza) supplemented with the EGM-2 BulletKit (Lonza) on pre-coated (coating solution containing 0.02 % gelatin from bovine skin type B and 125 ng/ml fibronectin from bovine plasma; both Merck Chemicals, Darmstadt; Germany) cell culture flasks.