・ xho i  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    New England Biolabs ・ xho i
    ・ Xho I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/・ xho i/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ・ xho i - by Bioz Stars, 2020-08
    86/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

    Nucleic Acid Electrophoresis:

    Article Title:
    Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

    Agarose Gel Electrophoresis:

    Article Title:
    Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

    Blocking Assay:

    Article Title:
    Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs xho i
    <t>Xho</t> I digests of miniprep <t>DNA.</t> Each DNA sample was digested with Xho I and electrophoresed on a 0.8% agarose gel. Lanes 1–7 represent various transformants. Lane 8 is a 1 kb ladder
    Xho I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xho i/product/New England Biolabs
    Average 99 stars, based on 321 article reviews
    Price from $9.99 to $1999.99
    xho i - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    New England Biolabs xho i site
    Expression constructs with various LTSM - TSS distances . (A) Schema of the vector construct pRPL18: the promoter region of RPL18 containing an internal start codon (ATG) was cloned into the gene trap vector pT1β-geo in frame with the β-geo gene lacking its start codon. An <t>Xho</t> I restriction site was added to facilitate the insertion of linker sequences of different lengths. (B, C, D) The cells were transfected with a GFP control construct (pGFP) and the five β-geo constructs, one with the endogenous RPL18 promoter including the Xho I site (pRPL18- Xho ) and four with additional linkers of the lengths 4 bp, 29 bp, 53 bp and 117 bp. (B) Northern and Western blotting analyses of β-geo mRNA and protein using a β-geo specific DNA probe and an anti-β-Galactosidase antibody in the respective experiment. (C) One representative X-Gal staining of cells transfected with the different constructs. (D) Overlay of the results of three measures of expression of the construct: mRNA, protein, and enzymatic activity (see B, C). The images were scanned and the signals quantified using ImageQuant. Northern blotting signals were normalized to the rRNA fluorescence intensities of the agarose gels (not shown) and Western blotting signals by reprobing the membranes with an anti-β-actin antibody (not shown). For each experiment the signal of pRPL18- Xho was assigned 100% and the other signals were quantified relative to it.
    Xho I Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xho i site/product/New England Biolabs
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    xho i site - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Xho I digests of miniprep DNA. Each DNA sample was digested with Xho I and electrophoresed on a 0.8% agarose gel. Lanes 1–7 represent various transformants. Lane 8 is a 1 kb ladder

    Journal: BMC Research Notes

    Article Title: Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site

    doi: 10.1186/s13104-017-2631-8

    Figure Lengend Snippet: Xho I digests of miniprep DNA. Each DNA sample was digested with Xho I and electrophoresed on a 0.8% agarose gel. Lanes 1–7 represent various transformants. Lane 8 is a 1 kb ladder

    Article Snippet: The DNA samples were digested with 10 U of Xho I for 2 h at 37 °C (New England BioLabs) and electrophoresed in a 0.8% agarose gel in 0.5X TBE.

    Techniques: Agarose Gel Electrophoresis

    Design of the mutant loxP oligonucleotide. The 13 bp inverted repeats are underlined . The mutated bases in the 8 bp central spacer are shown in shadowed grey font . The Xho I site is also indicated

    Journal: BMC Research Notes

    Article Title: Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site

    doi: 10.1186/s13104-017-2631-8

    Figure Lengend Snippet: Design of the mutant loxP oligonucleotide. The 13 bp inverted repeats are underlined . The mutated bases in the 8 bp central spacer are shown in shadowed grey font . The Xho I site is also indicated

    Article Snippet: The DNA samples were digested with 10 U of Xho I for 2 h at 37 °C (New England BioLabs) and electrophoresed in a 0.8% agarose gel in 0.5X TBE.

    Techniques: Mutagenesis

    STAT1 P696S would be associated with an ESE defect. ( A ). Nucleotides from exon 23 are shown, with the C2086 nucleotide in the WT sequence and the C2086T substitution in the P696S sequence shown in red. The horizontal blue and green bars show the significance threshold homology score for the binding of SC35 and SRp40 proteins, respectively. The predicted binding sites of these proteins are shown as rectangles along the length of the nucleotide sequence at the height of the homology score. ( B ) The genomic STAT1 region from nucleotide 36989 to 38523 (NC_000002) was inserted into an exon-trapping vector using Xho I and BamH 1, with or without the C2086T (P696S) nucleotide substitution. The exons are numbered in Roman numerals and shown in gray boxes, with the introns between them in white boxes, with the exception of exon 23, which is shown in a red box. The vector is shown as black boxes. HEK293 and COS-7 cells were transfected with the various constructs, the exon-trapping pSPL3 mock vector (pmock-p), or no vector (–). RNA was isolated, and the various spliced products were amplified and are shown on an agarose gel with GADPH amplification. The various products were isolated and sequenced, and the resulting sequences are also shown with corresponding exons and MW. These results are representative of 2 independent experiments.

    Journal: The Journal of Clinical Investigation

    Article Title: A partial form of recessive STAT1 deficiency in humans

    doi: 10.1172/JCI37083

    Figure Lengend Snippet: STAT1 P696S would be associated with an ESE defect. ( A ). Nucleotides from exon 23 are shown, with the C2086 nucleotide in the WT sequence and the C2086T substitution in the P696S sequence shown in red. The horizontal blue and green bars show the significance threshold homology score for the binding of SC35 and SRp40 proteins, respectively. The predicted binding sites of these proteins are shown as rectangles along the length of the nucleotide sequence at the height of the homology score. ( B ) The genomic STAT1 region from nucleotide 36989 to 38523 (NC_000002) was inserted into an exon-trapping vector using Xho I and BamH 1, with or without the C2086T (P696S) nucleotide substitution. The exons are numbered in Roman numerals and shown in gray boxes, with the introns between them in white boxes, with the exception of exon 23, which is shown in a red box. The vector is shown as black boxes. HEK293 and COS-7 cells were transfected with the various constructs, the exon-trapping pSPL3 mock vector (pmock-p), or no vector (–). RNA was isolated, and the various spliced products were amplified and are shown on an agarose gel with GADPH amplification. The various products were isolated and sequenced, and the resulting sequences are also shown with corresponding exons and MW. These results are representative of 2 independent experiments.

    Article Snippet: PCR products and empty pSPL3 plasmids (provided by Ralph Burkhardt, The Rockefeller University) were digested with Xho I and BamH 1 (New England BioLabs).

    Techniques: Sequencing, Binding Assay, Plasmid Preparation, Transfection, Construct, Isolation, Amplification, Agarose Gel Electrophoresis

    Southern blots of tomato genomic DNA hybridized with full-length cDNAs of LeMAN1 (left) and LeMAN2 (right). Genomic DNA (10 μg) isolated from tomato leaves was digested with Bam HI ( Bam ), Xba I ( Xba ), and Xho I ( Xho ). Bands marked with arrows hybridized more strongly with LeMAN1 , whereas the band marked with an arrowhead hybridized more strongly to LeMAN2 (see text for details).

    Journal: Plant Physiology

    Article Title: A Germination-Specific Endo-?-Mannanase Gene Is Expressed in the Micropylar Endosperm Cap of Tomato Seeds 1

    doi:

    Figure Lengend Snippet: Southern blots of tomato genomic DNA hybridized with full-length cDNAs of LeMAN1 (left) and LeMAN2 (right). Genomic DNA (10 μg) isolated from tomato leaves was digested with Bam HI ( Bam ), Xba I ( Xba ), and Xho I ( Xho ). Bands marked with arrows hybridized more strongly with LeMAN1 , whereas the band marked with an arrowhead hybridized more strongly to LeMAN2 (see text for details).

    Article Snippet: Genomic DNA (10 μg) was digested with the restriction enzymes Bam HI, Xba I, and Xho I (New England Biolabs), separated on a 1.0% (w/v) agarose gel, and transferred to positively charged membranes (Hybond-N+ , Amersham Pharmacia Biotech).

    Techniques: Isolation

    Expression constructs with various LTSM - TSS distances . (A) Schema of the vector construct pRPL18: the promoter region of RPL18 containing an internal start codon (ATG) was cloned into the gene trap vector pT1β-geo in frame with the β-geo gene lacking its start codon. An Xho I restriction site was added to facilitate the insertion of linker sequences of different lengths. (B, C, D) The cells were transfected with a GFP control construct (pGFP) and the five β-geo constructs, one with the endogenous RPL18 promoter including the Xho I site (pRPL18- Xho ) and four with additional linkers of the lengths 4 bp, 29 bp, 53 bp and 117 bp. (B) Northern and Western blotting analyses of β-geo mRNA and protein using a β-geo specific DNA probe and an anti-β-Galactosidase antibody in the respective experiment. (C) One representative X-Gal staining of cells transfected with the different constructs. (D) Overlay of the results of three measures of expression of the construct: mRNA, protein, and enzymatic activity (see B, C). The images were scanned and the signals quantified using ImageQuant. Northern blotting signals were normalized to the rRNA fluorescence intensities of the agarose gels (not shown) and Western blotting signals by reprobing the membranes with an anti-β-actin antibody (not shown). For each experiment the signal of pRPL18- Xho was assigned 100% and the other signals were quantified relative to it.

    Journal: BMC Genomics

    Article Title: A tandem sequence motif acts as a distance-dependent enhancer in a set of genes involved in translation by binding the proteins NonO and SFPQ

    doi: 10.1186/1471-2164-12-624

    Figure Lengend Snippet: Expression constructs with various LTSM - TSS distances . (A) Schema of the vector construct pRPL18: the promoter region of RPL18 containing an internal start codon (ATG) was cloned into the gene trap vector pT1β-geo in frame with the β-geo gene lacking its start codon. An Xho I restriction site was added to facilitate the insertion of linker sequences of different lengths. (B, C, D) The cells were transfected with a GFP control construct (pGFP) and the five β-geo constructs, one with the endogenous RPL18 promoter including the Xho I site (pRPL18- Xho ) and four with additional linkers of the lengths 4 bp, 29 bp, 53 bp and 117 bp. (B) Northern and Western blotting analyses of β-geo mRNA and protein using a β-geo specific DNA probe and an anti-β-Galactosidase antibody in the respective experiment. (C) One representative X-Gal staining of cells transfected with the different constructs. (D) Overlay of the results of three measures of expression of the construct: mRNA, protein, and enzymatic activity (see B, C). The images were scanned and the signals quantified using ImageQuant. Northern blotting signals were normalized to the rRNA fluorescence intensities of the agarose gels (not shown) and Western blotting signals by reprobing the membranes with an anti-β-actin antibody (not shown). For each experiment the signal of pRPL18- Xho was assigned 100% and the other signals were quantified relative to it.

    Article Snippet: Three oligonucleotides linkers were generated with using the primer pairs Geo-link 25-fw/rev, Geo-link 50-fw/rev and Geo-link 75-fw/rev (Additional file ) and a fill-in of the Xho I site took place (T4 DNA polymerase, NEB) resulting in a relocation of the LTSM to position +4, +29, +53 and +117 related to the original position of +68 of the RPL18 LTSM.

    Techniques: Expressing, Construct, Plasmid Preparation, Clone Assay, Transfection, Northern Blot, Western Blot, Staining, Activity Assay, Fluorescence