•human cot1 dna  (Thermo Fisher)


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    Name:
    Human Cot 1 DNA Fluorometric QC
    Description:
    Human Cot 1 DNA Fluorometric QC is placental DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the Alu and Kpn family members 1 2 Quantifying Cot 1 DNA by fluorometry provides more accurate concentration data of higher molecular weight products making it beneficial to certain genomic based array experiments such as array CGH using BAC arrays Human Cot 1 DNA Fluorometric QC suppresses prevalent repetitive elements during hybridization improving results
    Catalog Number:
    15279101
    Price:
    None
    Applications:
    Cell Analysis|Cellular Imaging|ChIP-on-Chip|Fluorescence In Situ Hybridization|Genotyping & Genomic Profiling|In Situ Hybridization (ISH)|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology|Gene Expression Analysis & Genotyping|Array CGH|Microarray Hybridization & General Reagents
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher •human cot1 dna
    Principle of array-CGH. Genomic <t>DNA</t> from two cell populations is differentially labeled and hybridized to a microarray in the presence of <t>Cot1</t> DNA to suppress repeat sequences. The fluorescent ratios on each array spot are calculated and normalized so
    Human Cot 1 DNA Fluorometric QC is placental DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the Alu and Kpn family members 1 2 Quantifying Cot 1 DNA by fluorometry provides more accurate concentration data of higher molecular weight products making it beneficial to certain genomic based array experiments such as array CGH using BAC arrays Human Cot 1 DNA Fluorometric QC suppresses prevalent repetitive elements during hybridization improving results
    https://www.bioz.com/result/•human cot1 dna/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    •human cot1 dna - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes"

    Article Title: Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes

    Journal:

    doi: 10.1038/nprot.2007.53

    Principle of array-CGH. Genomic DNA from two cell populations is differentially labeled and hybridized to a microarray in the presence of Cot1 DNA to suppress repeat sequences. The fluorescent ratios on each array spot are calculated and normalized so
    Figure Legend Snippet: Principle of array-CGH. Genomic DNA from two cell populations is differentially labeled and hybridized to a microarray in the presence of Cot1 DNA to suppress repeat sequences. The fluorescent ratios on each array spot are calculated and normalized so

    Techniques Used: Labeling, Microarray

    Related Articles

    DNA Hybridization:

    Article Title: Gestational folic acid content alters the development and function of hypothalamic food intake regulating neurons in Wistar rat offspring post-weaning.
    Article Snippet: .. < b > Background < /b > : Folic acid plays an important role in early brain development of offspring, including proliferation and differentiation of neural stem cells known to impact the function of food intake regulatory pathways. .. < b > Background < /b > : Folic acid plays an important role in early brain development of offspring, including proliferation and differentiation of neural stem cells known to impact the function of food intake regulatory pathways.

    High Performance Liquid Chromatography:

    Article Title: Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes
    Article Snippet: .. •2× TY medium, pH 7.4 (see REAGENT SETUP) •Solution I (see REAGENT SETUP) •Solution II (see REAGENT SETUP) •Solution III (see REAGENT SETUP) •Isopropanol •70% ethanol •100% ethanol •80% ethanol •T0.1E solution, pH 8 (see REAGENT SETUP) •RNaseA (ICN Biochemicals, cat. no. 101076) •DOP-PCR primers: •DOP 1: 5′ CCGACTCGAGNNNNNNCTAGAA 3′ •DOP 2: 5′ CCGACTCGAGNNNNNNTAGGAG 3′ •DOP 3: 5′ CCGACTCGAGNNNNNNTTCTAG 3′ •TAPS ( N -[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (Sigma-Aldrich, cat. no. T5130) •TAPS salt solution (see REAGENT SETUP) •TAPS2 buffer (see REAGENT SETUP) •Amino-linked primer: •5′ GGAAACAGCCCGACTCGAG 3′ •Amplitaq polymerase, 5 U μl−1 (Roche, cat. no. N808-0145) •dNTPs (Amersham Biosciences, cat. no. 27-2035-01) •Amino-linking buffer (see REAGENT SETUP) •4× microarray spotting buffer: 1 M sodium phosphate buffer, pH 8.5, 0.001% sarkosyl •1% (w/v) ammonium hydroxide solution (prepared with HPLC water) •0.1% (w/v) sodium dodecyl sulfate solution (prepared with HPLC water) •HPCL water •BioPrime Labeling Kit (Invitrogen, cat. no. 18094011) •10× dNTP mix (1 mM dCTP, 2 mM dATP, 2 mM dGTP, 2 mM dTTP in TE buffer) •1 mM Cy3-dCTP (NEN Life Science, cat. no. NEL 576) •1 mM Cy5-dCTP (NEN Life Science, cat. no. NEL 577) •Manual array hybridization buffer (see REAGENT SETUP) •3 M NaAc, pH 5.2 •Human Cot1 DNA (Invitrogen, cat. no. 15279-101) •20% formamide solution •50% formamide/2× SSC •PBS/0.05% Tween 20 •Automated array pre/hybridization buffer (see REAGENT SETUP) •Herring sperm DNA (Sigma, cat. no. D7290) •0.1× SSC •PBS/0.05% Tween 20/2 mM cysteamine •Cysteamine (Sigma, cat. no. M9768) .. •Microscope slide rack •Microscope slide storage boxes •Microcon YM30 columns (Millipore/Amicon, cat. no. 42411) •1 ml deep-well microtiter plates (Beckman, cat. no. 267007) •“U” bottom microtiter plate (Greiner, cat. no. 650161) •PTC-225 Tetrad thermocyclers (MJ Research) •96-well PCR plates •2.5% agarose gels •Multiscreen 0.2-μm filter plate (Millipore, MSGVN 2250) •384-well plates (Genetix) •CodeLink activated slides (GE Healthcare UK Limited).

    Blocking Assay:

    Article Title: Ultra dense array CGH and discovery of micro-copy number alterations and gene fusions in cancer genome
    Article Snippet: .. Hybridization was set according to the protocol described in detail in the manual from Agilent (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, available on web) Prepare hybridization mix: 50 μl of Cot-1 DNA (1mg/ml, Invitrogen # 15279-101), 52 μl of 10 × blocking agent and 260 μl of 2 × Hi-RPM Buffer (Agilent Oligo aCGH Hybridization Kit # 5188-5380) and labeled DNAs (test and reference) Incubate 3 min at 95 °C Incubate 30 min at 37 °C Hybridize for 40 hrs at 65 °C Washing was performed with Washing Buffers from Agilent and according to their procedure, except the second wash at 37 °C was done for 3 minutes. ..

    Microarray:

    Article Title: Ultra dense array CGH and discovery of micro-copy number alterations and gene fusions in cancer genome
    Article Snippet: .. Hybridization chamber gasket slides, 5-pack (Agilent #G2534-60003) Hybridization oven (Agilent #G2545A) and Hybridization oven rotator for Agilent Microarray hybridization chambers (Agilent #G2530-60029) Hybridization Chambers (Agilent #G2534A) Ozone-barrier slide covers (Agilent #G2505-60550) Cot-1 DNA 1mg/ml (Invitrogen # 15279-101) Agilent Oligo aCGH Hybridization Kit (Agilent # 5188-5380) Agilent Oligo aCGH Wash Buffer 1 and 2 set (Agilent # 5188-522 250 ml rectangular slide staining dishes (Wheaton) 1.5L flat Pyrex baking dish for warming up the dish with washing buffer #2 ..

    Article Title: Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes
    Article Snippet: .. •2× TY medium, pH 7.4 (see REAGENT SETUP) •Solution I (see REAGENT SETUP) •Solution II (see REAGENT SETUP) •Solution III (see REAGENT SETUP) •Isopropanol •70% ethanol •100% ethanol •80% ethanol •T0.1E solution, pH 8 (see REAGENT SETUP) •RNaseA (ICN Biochemicals, cat. no. 101076) •DOP-PCR primers: •DOP 1: 5′ CCGACTCGAGNNNNNNCTAGAA 3′ •DOP 2: 5′ CCGACTCGAGNNNNNNTAGGAG 3′ •DOP 3: 5′ CCGACTCGAGNNNNNNTTCTAG 3′ •TAPS ( N -[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (Sigma-Aldrich, cat. no. T5130) •TAPS salt solution (see REAGENT SETUP) •TAPS2 buffer (see REAGENT SETUP) •Amino-linked primer: •5′ GGAAACAGCCCGACTCGAG 3′ •Amplitaq polymerase, 5 U μl−1 (Roche, cat. no. N808-0145) •dNTPs (Amersham Biosciences, cat. no. 27-2035-01) •Amino-linking buffer (see REAGENT SETUP) •4× microarray spotting buffer: 1 M sodium phosphate buffer, pH 8.5, 0.001% sarkosyl •1% (w/v) ammonium hydroxide solution (prepared with HPLC water) •0.1% (w/v) sodium dodecyl sulfate solution (prepared with HPLC water) •HPCL water •BioPrime Labeling Kit (Invitrogen, cat. no. 18094011) •10× dNTP mix (1 mM dCTP, 2 mM dATP, 2 mM dGTP, 2 mM dTTP in TE buffer) •1 mM Cy3-dCTP (NEN Life Science, cat. no. NEL 576) •1 mM Cy5-dCTP (NEN Life Science, cat. no. NEL 577) •Manual array hybridization buffer (see REAGENT SETUP) •3 M NaAc, pH 5.2 •Human Cot1 DNA (Invitrogen, cat. no. 15279-101) •20% formamide solution •50% formamide/2× SSC •PBS/0.05% Tween 20 •Automated array pre/hybridization buffer (see REAGENT SETUP) •Herring sperm DNA (Sigma, cat. no. D7290) •0.1× SSC •PBS/0.05% Tween 20/2 mM cysteamine •Cysteamine (Sigma, cat. no. M9768) .. •Microscope slide rack •Microscope slide storage boxes •Microcon YM30 columns (Millipore/Amicon, cat. no. 42411) •1 ml deep-well microtiter plates (Beckman, cat. no. 267007) •“U” bottom microtiter plate (Greiner, cat. no. 650161) •PTC-225 Tetrad thermocyclers (MJ Research) •96-well PCR plates •2.5% agarose gels •Multiscreen 0.2-μm filter plate (Millipore, MSGVN 2250) •384-well plates (Genetix) •CodeLink activated slides (GE Healthcare UK Limited).

    other:

    Article Title: Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms.
    Article Snippet: These studies describe the effects of nanosecond (10-300 ns) pulsed electric fields (nsPEF) on mammalian cell structure and function.

    Labeling:

    Article Title: Ultra dense array CGH and discovery of micro-copy number alterations and gene fusions in cancer genome
    Article Snippet: .. Hybridization was set according to the protocol described in detail in the manual from Agilent (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, available on web) Prepare hybridization mix: 50 μl of Cot-1 DNA (1mg/ml, Invitrogen # 15279-101), 52 μl of 10 × blocking agent and 260 μl of 2 × Hi-RPM Buffer (Agilent Oligo aCGH Hybridization Kit # 5188-5380) and labeled DNAs (test and reference) Incubate 3 min at 95 °C Incubate 30 min at 37 °C Hybridize for 40 hrs at 65 °C Washing was performed with Washing Buffers from Agilent and according to their procedure, except the second wash at 37 °C was done for 3 minutes. ..

    Article Title: Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes
    Article Snippet: .. •2× TY medium, pH 7.4 (see REAGENT SETUP) •Solution I (see REAGENT SETUP) •Solution II (see REAGENT SETUP) •Solution III (see REAGENT SETUP) •Isopropanol •70% ethanol •100% ethanol •80% ethanol •T0.1E solution, pH 8 (see REAGENT SETUP) •RNaseA (ICN Biochemicals, cat. no. 101076) •DOP-PCR primers: •DOP 1: 5′ CCGACTCGAGNNNNNNCTAGAA 3′ •DOP 2: 5′ CCGACTCGAGNNNNNNTAGGAG 3′ •DOP 3: 5′ CCGACTCGAGNNNNNNTTCTAG 3′ •TAPS ( N -[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (Sigma-Aldrich, cat. no. T5130) •TAPS salt solution (see REAGENT SETUP) •TAPS2 buffer (see REAGENT SETUP) •Amino-linked primer: •5′ GGAAACAGCCCGACTCGAG 3′ •Amplitaq polymerase, 5 U μl−1 (Roche, cat. no. N808-0145) •dNTPs (Amersham Biosciences, cat. no. 27-2035-01) •Amino-linking buffer (see REAGENT SETUP) •4× microarray spotting buffer: 1 M sodium phosphate buffer, pH 8.5, 0.001% sarkosyl •1% (w/v) ammonium hydroxide solution (prepared with HPLC water) •0.1% (w/v) sodium dodecyl sulfate solution (prepared with HPLC water) •HPCL water •BioPrime Labeling Kit (Invitrogen, cat. no. 18094011) •10× dNTP mix (1 mM dCTP, 2 mM dATP, 2 mM dGTP, 2 mM dTTP in TE buffer) •1 mM Cy3-dCTP (NEN Life Science, cat. no. NEL 576) •1 mM Cy5-dCTP (NEN Life Science, cat. no. NEL 577) •Manual array hybridization buffer (see REAGENT SETUP) •3 M NaAc, pH 5.2 •Human Cot1 DNA (Invitrogen, cat. no. 15279-101) •20% formamide solution •50% formamide/2× SSC •PBS/0.05% Tween 20 •Automated array pre/hybridization buffer (see REAGENT SETUP) •Herring sperm DNA (Sigma, cat. no. D7290) •0.1× SSC •PBS/0.05% Tween 20/2 mM cysteamine •Cysteamine (Sigma, cat. no. M9768) .. •Microscope slide rack •Microscope slide storage boxes •Microcon YM30 columns (Millipore/Amicon, cat. no. 42411) •1 ml deep-well microtiter plates (Beckman, cat. no. 267007) •“U” bottom microtiter plate (Greiner, cat. no. 650161) •PTC-225 Tetrad thermocyclers (MJ Research) •96-well PCR plates •2.5% agarose gels •Multiscreen 0.2-μm filter plate (Millipore, MSGVN 2250) •384-well plates (Genetix) •CodeLink activated slides (GE Healthcare UK Limited).

    Staining:

    Article Title: Ultra dense array CGH and discovery of micro-copy number alterations and gene fusions in cancer genome
    Article Snippet: .. Hybridization chamber gasket slides, 5-pack (Agilent #G2534-60003) Hybridization oven (Agilent #G2545A) and Hybridization oven rotator for Agilent Microarray hybridization chambers (Agilent #G2530-60029) Hybridization Chambers (Agilent #G2534A) Ozone-barrier slide covers (Agilent #G2505-60550) Cot-1 DNA 1mg/ml (Invitrogen # 15279-101) Agilent Oligo aCGH Hybridization Kit (Agilent # 5188-5380) Agilent Oligo aCGH Wash Buffer 1 and 2 set (Agilent # 5188-522 250 ml rectangular slide staining dishes (Wheaton) 1.5L flat Pyrex baking dish for warming up the dish with washing buffer #2 ..

    Hybridization:

    Article Title: Ultra dense array CGH and discovery of micro-copy number alterations and gene fusions in cancer genome
    Article Snippet: .. Hybridization chamber gasket slides, 5-pack (Agilent #G2534-60003) Hybridization oven (Agilent #G2545A) and Hybridization oven rotator for Agilent Microarray hybridization chambers (Agilent #G2530-60029) Hybridization Chambers (Agilent #G2534A) Ozone-barrier slide covers (Agilent #G2505-60550) Cot-1 DNA 1mg/ml (Invitrogen # 15279-101) Agilent Oligo aCGH Hybridization Kit (Agilent # 5188-5380) Agilent Oligo aCGH Wash Buffer 1 and 2 set (Agilent # 5188-522 250 ml rectangular slide staining dishes (Wheaton) 1.5L flat Pyrex baking dish for warming up the dish with washing buffer #2 ..

    Article Title: Ultra dense array CGH and discovery of micro-copy number alterations and gene fusions in cancer genome
    Article Snippet: .. Hybridization was set according to the protocol described in detail in the manual from Agilent (Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, available on web) Prepare hybridization mix: 50 μl of Cot-1 DNA (1mg/ml, Invitrogen # 15279-101), 52 μl of 10 × blocking agent and 260 μl of 2 × Hi-RPM Buffer (Agilent Oligo aCGH Hybridization Kit # 5188-5380) and labeled DNAs (test and reference) Incubate 3 min at 95 °C Incubate 30 min at 37 °C Hybridize for 40 hrs at 65 °C Washing was performed with Washing Buffers from Agilent and according to their procedure, except the second wash at 37 °C was done for 3 minutes. ..

    Article Title: Gestational folic acid content alters the development and function of hypothalamic food intake regulating neurons in Wistar rat offspring post-weaning.
    Article Snippet: .. < b > Background < /b > : Folic acid plays an important role in early brain development of offspring, including proliferation and differentiation of neural stem cells known to impact the function of food intake regulatory pathways. .. < b > Background < /b > : Folic acid plays an important role in early brain development of offspring, including proliferation and differentiation of neural stem cells known to impact the function of food intake regulatory pathways.

    Article Title: Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes
    Article Snippet: .. •2× TY medium, pH 7.4 (see REAGENT SETUP) •Solution I (see REAGENT SETUP) •Solution II (see REAGENT SETUP) •Solution III (see REAGENT SETUP) •Isopropanol •70% ethanol •100% ethanol •80% ethanol •T0.1E solution, pH 8 (see REAGENT SETUP) •RNaseA (ICN Biochemicals, cat. no. 101076) •DOP-PCR primers: •DOP 1: 5′ CCGACTCGAGNNNNNNCTAGAA 3′ •DOP 2: 5′ CCGACTCGAGNNNNNNTAGGAG 3′ •DOP 3: 5′ CCGACTCGAGNNNNNNTTCTAG 3′ •TAPS ( N -[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (Sigma-Aldrich, cat. no. T5130) •TAPS salt solution (see REAGENT SETUP) •TAPS2 buffer (see REAGENT SETUP) •Amino-linked primer: •5′ GGAAACAGCCCGACTCGAG 3′ •Amplitaq polymerase, 5 U μl−1 (Roche, cat. no. N808-0145) •dNTPs (Amersham Biosciences, cat. no. 27-2035-01) •Amino-linking buffer (see REAGENT SETUP) •4× microarray spotting buffer: 1 M sodium phosphate buffer, pH 8.5, 0.001% sarkosyl •1% (w/v) ammonium hydroxide solution (prepared with HPLC water) •0.1% (w/v) sodium dodecyl sulfate solution (prepared with HPLC water) •HPCL water •BioPrime Labeling Kit (Invitrogen, cat. no. 18094011) •10× dNTP mix (1 mM dCTP, 2 mM dATP, 2 mM dGTP, 2 mM dTTP in TE buffer) •1 mM Cy3-dCTP (NEN Life Science, cat. no. NEL 576) •1 mM Cy5-dCTP (NEN Life Science, cat. no. NEL 577) •Manual array hybridization buffer (see REAGENT SETUP) •3 M NaAc, pH 5.2 •Human Cot1 DNA (Invitrogen, cat. no. 15279-101) •20% formamide solution •50% formamide/2× SSC •PBS/0.05% Tween 20 •Automated array pre/hybridization buffer (see REAGENT SETUP) •Herring sperm DNA (Sigma, cat. no. D7290) •0.1× SSC •PBS/0.05% Tween 20/2 mM cysteamine •Cysteamine (Sigma, cat. no. M9768) .. •Microscope slide rack •Microscope slide storage boxes •Microcon YM30 columns (Millipore/Amicon, cat. no. 42411) •1 ml deep-well microtiter plates (Beckman, cat. no. 267007) •“U” bottom microtiter plate (Greiner, cat. no. 650161) •PTC-225 Tetrad thermocyclers (MJ Research) •96-well PCR plates •2.5% agarose gels •Multiscreen 0.2-μm filter plate (Millipore, MSGVN 2250) •384-well plates (Genetix) •CodeLink activated slides (GE Healthcare UK Limited).