Structured Review

Millipore σ sirna
σ Sirna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/σ sirna/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
σ sirna - by Bioz Stars, 2020-07
99/100 stars

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Irradiation:

Article Title: A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
Article Snippet: .. We irradiated (5 Gy) siRNA-transfected U2OS cells and incubated them in medium containing 100 ng ml−1 nocodazole (Sigma) for 24 h. We fixed cells in 4% (v/v) formaldehyde and permeabilized them in cold (−20 °C) 90% (v/v) methanol. .. We seeded siRNA-U2OS cells (3 × 103 per 200 μl) into octuplicate microtiter wells, incubated them overnight, and then treated them with CPT for 48 h. We measured viability as described , .

Transfection:

Article Title: Increased activity of chondrocyte translational apparatus accompanies osteoarthritis
Article Snippet: .. Transfections with mTOR siRNA (nt2381; Sigma) were performed using Lipofectamine RNAiMax according to the manufacturer's protocol. .. MISSION siRNA Universal Negative Control was used as a control.

Article Title: Cross-talk among writers, readers, and erasers of m6A regulates cancer growth and progression
Article Snippet: .. Cancer cell lines were transfected with scrambled-siRNA or METTL14-siRNA/ALKBH5-siRNA (Sigma-Aldrich) for 48 or 72 hours before they were subjected to qRT-PCR or Western blot analysis, as described previously ( ). .. Antibodies against ALKBH5 (#HPA 007196), β-actin (#A3854), GAPDH (#G9295), and METTL14 (#HPA038002) were purchased from Sigma-Aldrich.

Article Title: Tissue Specific Roles for the Ribosome Biogenesis Factor Wdr43 in Zebrafish Development
Article Snippet: .. Having defined an important role for zebrafish Wdr43 in ribosome biogenesis, we further examined its function in cultured human HeLa cells, which we transfected with human WDR43 small interfering RNA (siRNA) (Sigma MISSION esiRNA) to silence WDR43 expression. .. GFP esiRNA was used as a negative control for these studies.

Incubation:

Article Title: A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
Article Snippet: .. We irradiated (5 Gy) siRNA-transfected U2OS cells and incubated them in medium containing 100 ng ml−1 nocodazole (Sigma) for 24 h. We fixed cells in 4% (v/v) formaldehyde and permeabilized them in cold (−20 °C) 90% (v/v) methanol. .. We seeded siRNA-U2OS cells (3 × 103 per 200 μl) into octuplicate microtiter wells, incubated them overnight, and then treated them with CPT for 48 h. We measured viability as described , .

Article Title: Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma
Article Snippet: .. To cap the siRNA loaded pSiNPs with PEI, the pellet was then resuspended in 0.05% PEI of 25 kDa (Sigma-Aldrich, 408727) diluted in EtOH and incubated for 20 min. pSiNPs were centrifuged, and the pellet was washed with EtOH for three times to wash off excess PEI. .. To measure the loading efficiency of siRNA into pSiNPs, siRNA concentration in the supernatant was measured at 260 nm by using a Nanodrop spectrophotometer (ND2000, ThermoFisher) and the amount of siRNA was then back calculated.

esiRNA:

Article Title: Tissue Specific Roles for the Ribosome Biogenesis Factor Wdr43 in Zebrafish Development
Article Snippet: .. Having defined an important role for zebrafish Wdr43 in ribosome biogenesis, we further examined its function in cultured human HeLa cells, which we transfected with human WDR43 small interfering RNA (siRNA) (Sigma MISSION esiRNA) to silence WDR43 expression. .. GFP esiRNA was used as a negative control for these studies.

Negative Control:

Article Title: Fidgetin-like 2: a microtubule-based regulator of wound healing
Article Snippet: .. The hydrolyzed TMOS (100 µl) was added to 900 µl of 20 µM of siRNA (mouse FL2 (Sigma-Aldrich, SASI_Mm02_00354635) or the negative control) solution containing 10 mM phosphate, pH 7.4. ..

Cell Culture:

Article Title: Tissue Specific Roles for the Ribosome Biogenesis Factor Wdr43 in Zebrafish Development
Article Snippet: .. Having defined an important role for zebrafish Wdr43 in ribosome biogenesis, we further examined its function in cultured human HeLa cells, which we transfected with human WDR43 small interfering RNA (siRNA) (Sigma MISSION esiRNA) to silence WDR43 expression. .. GFP esiRNA was used as a negative control for these studies.

Ubiquitin Assay:

Article Title: Rescue of ?F508-CFTR by the SGK1/Nedd4-2 Signaling Pathway *
Article Snippet: .. For the CFTR ubiquitination assay, drug- or siRNA-treated cells were lysed in radioimmune precipitation assay buffer supplemented with 10 μ m MG132, a proteasome inhibitor (Sigma-Aldrich). .. After immunoprecipitation with anti-CFTR C-terminal antibody, the immunoprecipitated proteins were eluted, separated on 6% SDS-PAGE gels, and transferred to polyvinylidene difluoride membranes.

Small Interfering RNA:

Article Title: Tissue Specific Roles for the Ribosome Biogenesis Factor Wdr43 in Zebrafish Development
Article Snippet: .. Having defined an important role for zebrafish Wdr43 in ribosome biogenesis, we further examined its function in cultured human HeLa cells, which we transfected with human WDR43 small interfering RNA (siRNA) (Sigma MISSION esiRNA) to silence WDR43 expression. .. GFP esiRNA was used as a negative control for these studies.

Quantitative RT-PCR:

Article Title: Cross-talk among writers, readers, and erasers of m6A regulates cancer growth and progression
Article Snippet: .. Cancer cell lines were transfected with scrambled-siRNA or METTL14-siRNA/ALKBH5-siRNA (Sigma-Aldrich) for 48 or 72 hours before they were subjected to qRT-PCR or Western blot analysis, as described previously ( ). .. Antibodies against ALKBH5 (#HPA 007196), β-actin (#A3854), GAPDH (#G9295), and METTL14 (#HPA038002) were purchased from Sigma-Aldrich.

Expressing:

Article Title: Tissue Specific Roles for the Ribosome Biogenesis Factor Wdr43 in Zebrafish Development
Article Snippet: .. Having defined an important role for zebrafish Wdr43 in ribosome biogenesis, we further examined its function in cultured human HeLa cells, which we transfected with human WDR43 small interfering RNA (siRNA) (Sigma MISSION esiRNA) to silence WDR43 expression. .. GFP esiRNA was used as a negative control for these studies.

Western Blot:

Article Title: Cross-talk among writers, readers, and erasers of m6A regulates cancer growth and progression
Article Snippet: .. Cancer cell lines were transfected with scrambled-siRNA or METTL14-siRNA/ALKBH5-siRNA (Sigma-Aldrich) for 48 or 72 hours before they were subjected to qRT-PCR or Western blot analysis, as described previously ( ). .. Antibodies against ALKBH5 (#HPA 007196), β-actin (#A3854), GAPDH (#G9295), and METTL14 (#HPA038002) were purchased from Sigma-Aldrich.

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    Millipore ribojuice sirna transfection reagent
    Ribojuice Sirna Transfection Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribojuice sirna transfection reagent/product/Millipore
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    ribojuice sirna transfection reagent - by Bioz Stars, 2020-07
    99/100 stars
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