λ exonuclease fermentas digestion  (Thermo Fisher)


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  • 95
    Name:
    Lambda Exonuclease 10 U µL
    Description:
    Thermo Scientific Lambda Exonuclease is a highly processive 5 →3 exodeoxyribonuclease It selectively digests the 5 phosphorylated strand of double stranded DNA The enzyme exhibits low activity on single stranded DNA and non phosphorylated DNA and has no activity at nicks and limited activity at gaps in DNA Highlights• Active in Thermo Scientific PCR buffersApplications• Generating single stranded PCR products for use in • DNA sequencing• Analysis of DNA single strand conformation polymorphism SSCP • Rolling circle amplification• Producing single stranded DNA from double stranded DNA fragments• Cloning of PCR productsNoteUse of this enzyme in certain applications may be covered by patents and may require a license
    Catalog Number:
    en0561
    Price:
    None
    Applications:
    Cloning|PCR Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher λ exonuclease fermentas digestion
    Thermo Scientific Lambda Exonuclease is a highly processive 5 →3 exodeoxyribonuclease It selectively digests the 5 phosphorylated strand of double stranded DNA The enzyme exhibits low activity on single stranded DNA and non phosphorylated DNA and has no activity at nicks and limited activity at gaps in DNA Highlights• Active in Thermo Scientific PCR buffersApplications• Generating single stranded PCR products for use in • DNA sequencing• Analysis of DNA single strand conformation polymorphism SSCP • Rolling circle amplification• Producing single stranded DNA from double stranded DNA fragments• Cloning of PCR productsNoteUse of this enzyme in certain applications may be covered by patents and may require a license
    https://www.bioz.com/result/λ exonuclease fermentas digestion/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    λ exonuclease fermentas digestion - by Bioz Stars, 2020-07
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Reciprocal regulation of expression of the human adenosine 5?-triphosphate binding cassette, sub-family A, transporter 2 (ABCA2) promoter by the early growth response-1 (EGR-1) and Sp-family transcription factors
    Article Snippet: .. Two micrograms of the PCR product was digested with λ exonuclease (Invitrogen) in 67 mM glycine–KOH pH 9.4, 2.5 mM MgCl2 , 50 µg/ml BSA at 37°C for 30 min to generate the antisense single-stranded DNA. .. The antisense single-stranded DNA was end labeled with [γ-32 P]ATP (10 mCi/ml; Amersham) and T4 polynucleotide kinase (Invitrogen).

    Sequencing:

    Article Title: Preferential Localization of Human Origins of DNA Replication at the 5?-Ends of Expressed Genes and at Evolutionarily Conserved DNA Sequences
    Article Snippet: .. After λ-exonuclease treatment of the DNA pool in the size range of 400–800 bp, we synthesized a double stranded DNA population required for massively parallel sequencing using the Klenow fragment of DNA polymerase I (Invitrogen, Carlsbed, CA) and random primers (Invitrogen, Carlsbad, CA). .. Random priming and DNA synthesis were performed according to the manufacturer's protocol except that the samples were incubated for an hour at 37°C.

    Incubation:

    Article Title: Stable Complexes Formed by HIV-1 Reverse Transcriptase at Distinct Positions on the Primer-Template Controlled by Binding Deoxynucleoside Triphosphates or Foscarnet
    Article Snippet: .. For upstream mapping of the borders of the stable complexes, 5 nM 3'-[32 P] ddAMP-terminated, 5'-phosphorylated L32 primer annealed to excess WL50 template was incubated with 200 nM HIV-1 RT and dNTP, foscarnet or no ligand as indicated in the figures, in 10 μl RB buffer for 15 min at 37°C, followed by incubation at 4°C for 5 min. After addition of 1.2 U lambda exonuclease (USB Corp.), the samples were incubated at 37°C for 8 min followed by heat-inactivation at 90°C for 5 min. For downstream mapping of the borders of the stable complexes, 5 nM 3'-[32 P]ddAMP-labeled WL65 template annealed to excess ddAMP-terminated L32 primer was incubated with 12.5 nM WT HIV-1 RT and ligand (0.8 mM dNTP, 3.2 mM foscarnet, or no ligand) in 10 μl RB buffer for 15 min at 37°C, followed by incubation at 4°C for 5 min. .. Ten units of RecJf (New England Biolabs) were added and the samples were incubated at 37°C for 10 min followed by heat-inactivation at 90°C for 5 min. For both upstream and downstream mapping, 12 μl loading buffer were added and the products were separated by electrophoresis through a denaturing 20% polyacrylamide gel.

    Article Title: Relaxed Primer Specificity Associated with Reverse Transcriptases Encoded by the pFOXC Retroplasmids of Fusarium oxysporum
    Article Snippet: .. In addition, products from a twofold-scaled-up reaction with the use of DEAE-purified mtRNP were divided and incubated at 37°C for 30 min either with or without 9 U of λ exonuclease (Invitrogen) in a reaction buffer containing 67 mM glycine (pH 9.4) and 2.5 mM MgCl2 . .. Previous studies demonstrate that pFOXC2 preparations isolated from bisbenzamide CsCl gradients are resistant to λ-exonuclease digestion following incubation periods of up to 3 h ( ).

    other:

    Article Title: Atomic Scissors: A New Method of Tracking the 5-Bromo-2?-Deoxyuridine-Labeled DNA In Situ
    Article Snippet: Enzymes used These enzymes and condition were used: Terminal deoxynucleotidyl transferase (TdT; 2 U/µl, 10 minutes, 37°C, Fermentas), buffer for TdT, 0.05 mM dATP, dGTP, dCTP and 0.05 mM Alexa Fluor® 555-aha-2′-deoxyuridine-5′-triphosphate (Alexa-dUTP); DNA polymerase I (0.2 U/µl, 10 minutes, RT, Fermentas), buffer for DNA polymerase I, 0.05 mM dATP, dGTP, dCTP and 0.05 mM Alexa-dUTP; Klenow fragment (0.2 U/µl, 10 minutes, RT, Fermentas), buffer for the Klenow fragment, 0.05 mM dATP, dGTP, dCTP and 0.05 mM Alexa-dUTP; Klenow fragment Exo- (0.2 U/µl, 10 minutes, RT, Fermentas), buffer for the Klenow fragment Exo-, 0.05 mM dATP, dGTP, dCTP and 0.05 mM Alexa-dUTP; Exonuclease III (1 U/µl, 30 minutes, RT, Fermentas), buffer for exonuclease III; Exonuclease λ (0.1 U/µl, 30 minutes, RT, Fermentas), buffer for exonuclease λ; Shrimp alkaline phosphomonoesterase (phosphatase; SAP; 1 U/µl, 20 minutes, 37°C, Fermentas), buffer for SAP.

    Synthesized:

    Article Title: Preferential Localization of Human Origins of DNA Replication at the 5?-Ends of Expressed Genes and at Evolutionarily Conserved DNA Sequences
    Article Snippet: .. After λ-exonuclease treatment of the DNA pool in the size range of 400–800 bp, we synthesized a double stranded DNA population required for massively parallel sequencing using the Klenow fragment of DNA polymerase I (Invitrogen, Carlsbed, CA) and random primers (Invitrogen, Carlsbad, CA). .. Random priming and DNA synthesis were performed according to the manufacturer's protocol except that the samples were incubated for an hour at 37°C.