β napthoflavone  (Millipore)


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  • 99

    Structured Review

    Millipore β napthoflavone
    Histochemical detection of β-glucosidase activity in mouse tissues . (a, b) Cryostat sections of snap frozen small intestine from non-transgenic mice stained with BCI-glu. (a) Endogenous activity in the villus brush-border. (b) No activity following incubation at 65°C for 20 min. (c-i) BCI-glu staining of tissues from R26 SYNbglA R (left of panel) and PGKcre m /R26 SYNbglA R (right of panel) mice: (c) heart and thymus, (d) spleen and pancreas, (e) kidney, (f) skeletal muscle, (g) liver, (h) glandular stomach and (i) brain. (j, k) Small intestinal wholemounts heat treated at 65°C and stained with BCI-glu from: (j) untreated Ahcre/R26 SYNbglA R; (k) <t>β-napthoflavone</t> treated Ahcre/R26 SYNbglA R mice (4 weeks post induction), showing detectable enzyme activity only in (k). (l-o) Wholemount of colon from an Ahcre/R26 SYNbglA R mouse prepared 16 weeks after β-napthoflavone treatment and stained with Mag-glu (p, proximal; d, distal). (l) Extensive recombination occurs throughout as indicated by magenta staining but becomes increasingly variegated towards the distal end. (m-o) Shows enlargements of areas indicated by arrows. Punctate magenta staining corresponds to individual colonic crypts. Bars: A, B 200 μm, C-O 1 cm.
    β Napthoflavone, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β napthoflavone/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    β napthoflavone - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Characterization of a heat resistant ss-glucosidase as a new reporter in cells and mice"

    Article Title: Characterization of a heat resistant ss-glucosidase as a new reporter in cells and mice

    Journal: BMC Biology

    doi: 10.1186/1741-7007-8-89

    Histochemical detection of β-glucosidase activity in mouse tissues . (a, b) Cryostat sections of snap frozen small intestine from non-transgenic mice stained with BCI-glu. (a) Endogenous activity in the villus brush-border. (b) No activity following incubation at 65°C for 20 min. (c-i) BCI-glu staining of tissues from R26 SYNbglA R (left of panel) and PGKcre m /R26 SYNbglA R (right of panel) mice: (c) heart and thymus, (d) spleen and pancreas, (e) kidney, (f) skeletal muscle, (g) liver, (h) glandular stomach and (i) brain. (j, k) Small intestinal wholemounts heat treated at 65°C and stained with BCI-glu from: (j) untreated Ahcre/R26 SYNbglA R; (k) β-napthoflavone treated Ahcre/R26 SYNbglA R mice (4 weeks post induction), showing detectable enzyme activity only in (k). (l-o) Wholemount of colon from an Ahcre/R26 SYNbglA R mouse prepared 16 weeks after β-napthoflavone treatment and stained with Mag-glu (p, proximal; d, distal). (l) Extensive recombination occurs throughout as indicated by magenta staining but becomes increasingly variegated towards the distal end. (m-o) Shows enlargements of areas indicated by arrows. Punctate magenta staining corresponds to individual colonic crypts. Bars: A, B 200 μm, C-O 1 cm.
    Figure Legend Snippet: Histochemical detection of β-glucosidase activity in mouse tissues . (a, b) Cryostat sections of snap frozen small intestine from non-transgenic mice stained with BCI-glu. (a) Endogenous activity in the villus brush-border. (b) No activity following incubation at 65°C for 20 min. (c-i) BCI-glu staining of tissues from R26 SYNbglA R (left of panel) and PGKcre m /R26 SYNbglA R (right of panel) mice: (c) heart and thymus, (d) spleen and pancreas, (e) kidney, (f) skeletal muscle, (g) liver, (h) glandular stomach and (i) brain. (j, k) Small intestinal wholemounts heat treated at 65°C and stained with BCI-glu from: (j) untreated Ahcre/R26 SYNbglA R; (k) β-napthoflavone treated Ahcre/R26 SYNbglA R mice (4 weeks post induction), showing detectable enzyme activity only in (k). (l-o) Wholemount of colon from an Ahcre/R26 SYNbglA R mouse prepared 16 weeks after β-napthoflavone treatment and stained with Mag-glu (p, proximal; d, distal). (l) Extensive recombination occurs throughout as indicated by magenta staining but becomes increasingly variegated towards the distal end. (m-o) Shows enlargements of areas indicated by arrows. Punctate magenta staining corresponds to individual colonic crypts. Bars: A, B 200 μm, C-O 1 cm.

    Techniques Used: Activity Assay, Transgenic Assay, Mouse Assay, Staining, Incubation

    Related Articles

    Injection:

    Article Title: Inflammation and Proliferation Act Together to Mediate Intestinal Cell Fusion
    Article Snippet: .. AhCre+ ; Apcfl/fl progeny were induced by intraperitoneal injection of β-naphthoflavone (β-NF; Sigma) dissolved in corn oil (80 mg/kg) for four days and analyzed 2 days later. .. For transplantation studies, β-NF injections were initiated two days post-transplant.

    Article Title: Intestinal stem cells lacking the Math1 tumour suppressor are refractory to Notch inhibitors
    Article Snippet: .. The Cre enzyme was induced by a single IP injection of 200 μl of β-naphthoflavone (10 mg ml−1 ; Sigma Aldrich) dissolved in corn oil on day 0. ..

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