Structured Review

Avantor β mercaptoethanol
β Mercaptoethanol, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β mercaptoethanol/product/Avantor
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
β mercaptoethanol - by Bioz Stars, 2020-08
92/100 stars

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Lysis:

Article Title: Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription
Article Snippet: .. Cells were thawed in a cell lysis buffer (CLB): 0.05 M Na-HEPES pH 7.5, 0.5 M NaCl, 0.02 M imidazole pH 8.0, 0.0005 M EDTA, 0.005 M β-ME and 0.01% (v/v) Brij35; the cell slurry was made 2 mg ml−1 in lysozyme (VWR). .. After 20 min, cells were sonicated and the clarified supernatant was applied in batch to Ni-NTA resin (Pierce) equilibrated with CLB.

other:

Article Title: Stapling of the botulinum type A protease to growth factors and neuropeptides allows selective targeting of neuroendocrine cells
Article Snippet: Min6 cells were grown in high glucose DMEM supplemented with 15% FCS, 1% P/S and 250 nM β-mercaptoethanol (VWR International, Lutterworth, UK).

Article Title: Glucose and GLP-1 Stimulate cAMP Production via Distinct Adenylyl Cyclases in INS-1E Insulinoma Cells
Article Snippet: RPMI media and HEPES were purchased from CellGrow, FBS from Gemini Bio-Products, and β-mercaptoethanol from JT Baker.

Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
Article Snippet: Protein samples were diluted in either 2x Laemmli sample buffer (Bio-Rad, Cat #161-0737) with or without β -mercaptoethanol (JT Baker, Cat #P62405) or 6x SDS-sample buffer (reducing) (Boston BioProducts, Cat #BP-111R).

Article Title: Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectin
Article Snippet: Isopentyl nitrite, 5-amino-3-(4-morpholinyl)-1,2,3-oxadiazolium chloride (SIN-1 chloride), HEPES, NaCl, EDTA, glycerol, β-mercaptoethanol were purchased from VWR.

Article Title: A Comprehensive Genome-Wide Map of Autonomously Replicating Sequences in a Naive Genome
Article Snippet: 2 mL of culture was pelleted and resuspended in 500 µL of a buffer consisting of 1 M sorbitol, 0.1M EDTA, 1 µL/mL β-mercaptoethanol, and 0.5 mg/mL Yeast Lytic Enzyme (VWR, catalog # IC360951).

Article Title: Identification of the transcriptional response of human intestinal mucosa to Lactobacillus plantarum WCFS1 in vivo
Article Snippet: Briefly, 1 mL TRIzol (Invitrogen Life Technologies b.v., Breda, The Netherlands) and 10 μL β-mercaptoethanol (VWR International b.v., Amsterdam, The Netherlands) were added to each frozen tissue sample and shaken with a minibeadbeater for 30 seconds.

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    Avantor β mercaptoethanol
    β Mercaptoethanol, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β mercaptoethanol/product/Avantor
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    β mercaptoethanol - by Bioz Stars, 2020-08
    92/100 stars
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