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Valiant β glycerophosphate
OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
β Glycerophosphate, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism"

Article Title: KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism

Journal: Redox Biology

doi: 10.1016/j.redox.2020.101622

OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using β-glycerophosphate plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
Figure Legend Snippet: OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using β-glycerophosphate plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p

Techniques Used: Expressing, Quantitative RT-PCR, Isolation

Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.
Figure Legend Snippet: Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.

Techniques Used: Cell Culture, Western Blot, Staining

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