Structured Review

Nacalai β glycerophosphate
β Glycerophosphate, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β glycerophosphate/product/Nacalai
Average 93 stars, based on 17 article reviews
Price from $9.99 to $1999.99
β glycerophosphate - by Bioz Stars, 2020-09
93/100 stars

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Related Articles

Transfection:

Article Title: Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension
Article Snippet: .. One day after transfection, cells were lysed in cell lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM β-glycerophosphate, 50 mM NaF, 1 mM Na3 VO4 , 2.5 mM sodium pyrophosphate, 2 mM EDTA, 1 mM EGTA and 1% Triton X-100) containing protease inhibitor cocktail (Nacalai Tesque). .. Cul3-3xFlag immunocomplexes immobilized on anti-DYKDDDDK-tag antibody beads (Wako Laboratory Chemicals) were washed three times with cell lysis buffer and twice with TBS (50 mM Tris–HCl (pH 7.4) 150 mM NaCl), and then eluted with 100 μg ml−1 3xFlag peptide (Sigma-Aldrich) dissolved in PBS containing 0.1% Tween 20 (PBST).

Protease Inhibitor:

Article Title: Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4
Article Snippet: .. Immunoprecipitation and Western Blotting HeLaS3 cells were washed in wash buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT), pelleted, resuspended in 1 ml of lysis buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT, 1% (v/v) NP40 and 0.5% (w/v) sodium deoxycholate, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate) containing a protease inhibitor cocktail (25955-11, Nacalai Tesque) and 1 mM PMSF and lysed for 30 min on ice. .. After centrifugation at 20,000× g for 10 min, the supernatant was collected and incubated for 3 h at 4°C with 50 µl anti-mouse IgG–conjugated Dynabeads (Veritas) that had been pre-incubated with 2 µg mouse anti-FLAG for 3 h at 4°C.

Article Title: Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension
Article Snippet: .. One day after transfection, cells were lysed in cell lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM β-glycerophosphate, 50 mM NaF, 1 mM Na3 VO4 , 2.5 mM sodium pyrophosphate, 2 mM EDTA, 1 mM EGTA and 1% Triton X-100) containing protease inhibitor cocktail (Nacalai Tesque). .. Cul3-3xFlag immunocomplexes immobilized on anti-DYKDDDDK-tag antibody beads (Wako Laboratory Chemicals) were washed three times with cell lysis buffer and twice with TBS (50 mM Tris–HCl (pH 7.4) 150 mM NaCl), and then eluted with 100 μg ml−1 3xFlag peptide (Sigma-Aldrich) dissolved in PBS containing 0.1% Tween 20 (PBST).

Article Title: PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3? and is required for metaphase-anaphase transition
Article Snippet: .. Identification of proteins bound to GST-14-3-3γ Mitotic and interphase HeLa cells were treated with cell lysis buffer (20 mM Tris-Cl (pH 7.5), 150 mM NaCl, 2 mM β-glycerophosphate, 50 mM NaF, 1 mM Na3 VO4 , 2.5 mM sodium pyrophosphate, 2 mM EDTA, 1 mM EGTA and 1% Triton X-100) containing protease inhibitor cocktail (Nacalai, Kyoto, Japan) for 30 min at 4 °C. .. After centrifugation (17,000g , 15 min), each cell extract (50 mg of total protein) was incubated with 100 μg of GST or GST-14-3-3γ for 2 h at 4 °C.

Article Title: Ablation of the p16INK4a tumour suppressor reverses ageing phenotypes of klotho mice
Article Snippet: .. Western blot analysis Tissue lysates were prepared using a homogenizer in lysis buffer (50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 10% glycerol, 0.1% Tween 20 and 10 mM β-glycerophosphate) containing protease inhibitor cocktail (Nacalai tesque) . .. The transferred membranes were immunoblotted directly with following antibodies, and the signals were detected using enhanced chemiluminescence system (GE Healthcare).

Article Title: Diverse distribution of tyrosine receptor kinase B isoforms in rat multiple tissues
Article Snippet: .. Protein lysates were obtained from mashed rat tissues using Cell Lysis Buffer (Cell Signaling Technology) containing 20 mM Tris-HCl (pH7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 and 1 µ g/ml leupeptin supplemented with 0.1% protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan) on ice. .. Protein concentration was determined by using a bicinchoninic acid method (Pierce, Rockford, IL, U.S.A.).

Immunoprecipitation:

Article Title: Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4
Article Snippet: .. Immunoprecipitation and Western Blotting HeLaS3 cells were washed in wash buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT), pelleted, resuspended in 1 ml of lysis buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT, 1% (v/v) NP40 and 0.5% (w/v) sodium deoxycholate, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate) containing a protease inhibitor cocktail (25955-11, Nacalai Tesque) and 1 mM PMSF and lysed for 30 min on ice. .. After centrifugation at 20,000× g for 10 min, the supernatant was collected and incubated for 3 h at 4°C with 50 µl anti-mouse IgG–conjugated Dynabeads (Veritas) that had been pre-incubated with 2 µg mouse anti-FLAG for 3 h at 4°C.

Article Title: PCTK1 Regulates Integrin-Dependent Spindle Orientation via Protein Kinase A Regulatory Subunit KAP0 and Myosin X
Article Snippet: .. For immunoprecipitation of FLAG-KAP0 and β1 integrin, M phase-synchronized cells were lysed with lysis buffer B (50 mM HEPES [pH 7.5], 2 mM EGTA, 2 mM MgCl2 , 12.5 mM β-glycerophosphate, 50 mM NaCl, 10% glycerol, 0.5% NP-40, 10 mM NaF, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 2 μg/ml aprotinin, and 100 μM okadaic acid) and centrifuged at 13,000 rpm at 4°C for 15 min. Anti-GFP antibody-conjugated beads (06083-05; Nacalai) were added to the supernatant, incubated at 4°C for 3 to 4 h, washed with lysis buffer B, and then subjected to immunoblotting. .. For immunoprecipitation of β1 integrin, mouse anti-β1 integrin antibodies (4 μg) were added to the supernatant and incubated at 4°C for 1 h. Protein G-Sepharose beads (GE Healthcare), preincubated with 3% BSA-phosphate-buffered saline (PBS), were added to the mixtures, incubated at 4°C for 2 to 3 h, washed three times with wash buffer (50 mM HEPES [pH 7.5], 2 mM EGTA, 2 mM MgCl2 , 12.5 mM β-glycerophosphate, 150 mM NaCl, 0.5% NP-40, 10 mM NaF, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 2 μg/ml aprotinin, and 100 μM okadaic acid), and then subjected to Western blotting.

Incubation:

Article Title: Characterization of Human Adipose Tissue-Resident Hematopoietic Cell Populations Reveals a Novel Macrophage Subpopulation with CD34 Expression and Mesenchymal Multipotency
Article Snippet: .. For osteogenic differentiation, cells were incubated 21 days in the DMEM containing 10% FBS supplemented with 0.1 mM dexamethasone, 50 mM ascorbate-2-phosphate, and 10 mM β-glycerophosphate (Nacalai Tesque). .. Osteogenic differentiation was confirmed by von Kossa staining.

Article Title: Adipose Injury-Associated Factors Mitigate Hypoxia in Ischemic Tissues through Activation of Adipose-Derived Stem/Progenitor/Stromal Cells and Induction of Angiogenesis
Article Snippet: .. For osteogenic differentiation, cells were incubated for 21 days in Dulbecco's modified Eagle's medium containing 10% FBS supplemented with 0.1 mM of dexamethasone, 50 mm of ascorbate-2-phosphate, and 10 mM of β-glycerophosphate (Nacalai Tesque, Kyoto, Japan). .. Osteogenic differentiation was visualized with von Kossa staining.

Article Title: PCTK1 Regulates Integrin-Dependent Spindle Orientation via Protein Kinase A Regulatory Subunit KAP0 and Myosin X
Article Snippet: .. For immunoprecipitation of FLAG-KAP0 and β1 integrin, M phase-synchronized cells were lysed with lysis buffer B (50 mM HEPES [pH 7.5], 2 mM EGTA, 2 mM MgCl2 , 12.5 mM β-glycerophosphate, 50 mM NaCl, 10% glycerol, 0.5% NP-40, 10 mM NaF, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 2 μg/ml aprotinin, and 100 μM okadaic acid) and centrifuged at 13,000 rpm at 4°C for 15 min. Anti-GFP antibody-conjugated beads (06083-05; Nacalai) were added to the supernatant, incubated at 4°C for 3 to 4 h, washed with lysis buffer B, and then subjected to immunoblotting. .. For immunoprecipitation of β1 integrin, mouse anti-β1 integrin antibodies (4 μg) were added to the supernatant and incubated at 4°C for 1 h. Protein G-Sepharose beads (GE Healthcare), preincubated with 3% BSA-phosphate-buffered saline (PBS), were added to the mixtures, incubated at 4°C for 2 to 3 h, washed three times with wash buffer (50 mM HEPES [pH 7.5], 2 mM EGTA, 2 mM MgCl2 , 12.5 mM β-glycerophosphate, 150 mM NaCl, 0.5% NP-40, 10 mM NaF, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 2 μg/ml aprotinin, and 100 μM okadaic acid), and then subjected to Western blotting.

Lysis:

Article Title: Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4
Article Snippet: .. Immunoprecipitation and Western Blotting HeLaS3 cells were washed in wash buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT), pelleted, resuspended in 1 ml of lysis buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT, 1% (v/v) NP40 and 0.5% (w/v) sodium deoxycholate, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate) containing a protease inhibitor cocktail (25955-11, Nacalai Tesque) and 1 mM PMSF and lysed for 30 min on ice. .. After centrifugation at 20,000× g for 10 min, the supernatant was collected and incubated for 3 h at 4°C with 50 µl anti-mouse IgG–conjugated Dynabeads (Veritas) that had been pre-incubated with 2 µg mouse anti-FLAG for 3 h at 4°C.

Article Title: Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension
Article Snippet: .. One day after transfection, cells were lysed in cell lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM β-glycerophosphate, 50 mM NaF, 1 mM Na3 VO4 , 2.5 mM sodium pyrophosphate, 2 mM EDTA, 1 mM EGTA and 1% Triton X-100) containing protease inhibitor cocktail (Nacalai Tesque). .. Cul3-3xFlag immunocomplexes immobilized on anti-DYKDDDDK-tag antibody beads (Wako Laboratory Chemicals) were washed three times with cell lysis buffer and twice with TBS (50 mM Tris–HCl (pH 7.4) 150 mM NaCl), and then eluted with 100 μg ml−1 3xFlag peptide (Sigma-Aldrich) dissolved in PBS containing 0.1% Tween 20 (PBST).

Article Title: PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3? and is required for metaphase-anaphase transition
Article Snippet: .. Identification of proteins bound to GST-14-3-3γ Mitotic and interphase HeLa cells were treated with cell lysis buffer (20 mM Tris-Cl (pH 7.5), 150 mM NaCl, 2 mM β-glycerophosphate, 50 mM NaF, 1 mM Na3 VO4 , 2.5 mM sodium pyrophosphate, 2 mM EDTA, 1 mM EGTA and 1% Triton X-100) containing protease inhibitor cocktail (Nacalai, Kyoto, Japan) for 30 min at 4 °C. .. After centrifugation (17,000g , 15 min), each cell extract (50 mg of total protein) was incubated with 100 μg of GST or GST-14-3-3γ for 2 h at 4 °C.

Article Title: PCTK1 Regulates Integrin-Dependent Spindle Orientation via Protein Kinase A Regulatory Subunit KAP0 and Myosin X
Article Snippet: .. For immunoprecipitation of FLAG-KAP0 and β1 integrin, M phase-synchronized cells were lysed with lysis buffer B (50 mM HEPES [pH 7.5], 2 mM EGTA, 2 mM MgCl2 , 12.5 mM β-glycerophosphate, 50 mM NaCl, 10% glycerol, 0.5% NP-40, 10 mM NaF, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 2 μg/ml aprotinin, and 100 μM okadaic acid) and centrifuged at 13,000 rpm at 4°C for 15 min. Anti-GFP antibody-conjugated beads (06083-05; Nacalai) were added to the supernatant, incubated at 4°C for 3 to 4 h, washed with lysis buffer B, and then subjected to immunoblotting. .. For immunoprecipitation of β1 integrin, mouse anti-β1 integrin antibodies (4 μg) were added to the supernatant and incubated at 4°C for 1 h. Protein G-Sepharose beads (GE Healthcare), preincubated with 3% BSA-phosphate-buffered saline (PBS), were added to the mixtures, incubated at 4°C for 2 to 3 h, washed three times with wash buffer (50 mM HEPES [pH 7.5], 2 mM EGTA, 2 mM MgCl2 , 12.5 mM β-glycerophosphate, 150 mM NaCl, 0.5% NP-40, 10 mM NaF, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 2 μg/ml aprotinin, and 100 μM okadaic acid), and then subjected to Western blotting.

Article Title: Ablation of the p16INK4a tumour suppressor reverses ageing phenotypes of klotho mice
Article Snippet: .. Western blot analysis Tissue lysates were prepared using a homogenizer in lysis buffer (50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 10% glycerol, 0.1% Tween 20 and 10 mM β-glycerophosphate) containing protease inhibitor cocktail (Nacalai tesque) . .. The transferred membranes were immunoblotted directly with following antibodies, and the signals were detected using enhanced chemiluminescence system (GE Healthcare).

Article Title: Diverse distribution of tyrosine receptor kinase B isoforms in rat multiple tissues
Article Snippet: .. Protein lysates were obtained from mashed rat tissues using Cell Lysis Buffer (Cell Signaling Technology) containing 20 mM Tris-HCl (pH7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 and 1 µ g/ml leupeptin supplemented with 0.1% protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan) on ice. .. Protein concentration was determined by using a bicinchoninic acid method (Pierce, Rockford, IL, U.S.A.).

Modification:

Article Title: Adipose Injury-Associated Factors Mitigate Hypoxia in Ischemic Tissues through Activation of Adipose-Derived Stem/Progenitor/Stromal Cells and Induction of Angiogenesis
Article Snippet: .. For osteogenic differentiation, cells were incubated for 21 days in Dulbecco's modified Eagle's medium containing 10% FBS supplemented with 0.1 mM of dexamethasone, 50 mm of ascorbate-2-phosphate, and 10 mM of β-glycerophosphate (Nacalai Tesque, Kyoto, Japan). .. Osteogenic differentiation was visualized with von Kossa staining.

Western Blot:

Article Title: Canonical Non-Homologous End Joining in Mitosis Induces Genome Instability and Is Suppressed by M-phase-Specific Phosphorylation of XRCC4
Article Snippet: .. Immunoprecipitation and Western Blotting HeLaS3 cells were washed in wash buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT), pelleted, resuspended in 1 ml of lysis buffer (50 mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM DTT, 1% (v/v) NP40 and 0.5% (w/v) sodium deoxycholate, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate) containing a protease inhibitor cocktail (25955-11, Nacalai Tesque) and 1 mM PMSF and lysed for 30 min on ice. .. After centrifugation at 20,000× g for 10 min, the supernatant was collected and incubated for 3 h at 4°C with 50 µl anti-mouse IgG–conjugated Dynabeads (Veritas) that had been pre-incubated with 2 µg mouse anti-FLAG for 3 h at 4°C.

Article Title: Ablation of the p16INK4a tumour suppressor reverses ageing phenotypes of klotho mice
Article Snippet: .. Western blot analysis Tissue lysates were prepared using a homogenizer in lysis buffer (50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 10% glycerol, 0.1% Tween 20 and 10 mM β-glycerophosphate) containing protease inhibitor cocktail (Nacalai tesque) . .. The transferred membranes were immunoblotted directly with following antibodies, and the signals were detected using enhanced chemiluminescence system (GE Healthcare).

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    Nacalai β glycerophosphate
    β Glycerophosphate, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Nacalai
    Average 93 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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