Structured Review

Merck & Co β glycerophosphate
β Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β glycerophosphate/product/Merck & Co
Average 93 stars, based on 12 article reviews
Price from $9.99 to $1999.99
β glycerophosphate - by Bioz Stars, 2020-09
93/100 stars

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Protease Inhibitor:

Article Title: ErbB2 regulates autophagic flux to modulate the proteostasis of APP-CTFs in Alzheimer’s disease
Article Snippet: .. PBS-washed cells were resuspended in a lysis buffer containing 1% Triton X-100, 50 mM Tris⋅HCl (pH 7.5), 150 mM NaCl, 25 μM β-glycerophosphate, 1 mM Na3 VO4 , and protease inhibitor mixture set V (Merck). .. Following the removal of cell debris by centrifugation, clarified lysates were processed for immunoprecipitation, Western blotting, or quantification of protein content.

Article Title: HP1 links centromeric heterochromatin to centromere cohesion in mammals
Article Snippet: .. For the co‐immunoprecipitation, cells were lysed in P150 buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 0.1% NP‐40, or 0.5% Triton X‐100) with 2 mM MgCl2 , 10% glycerol, 1 mM dithiothreitol (DTT), protease inhibitor cocktail (P8340, Sigma), 1 mM PMSF, 0.1 μM okadaic acid (Calbiochem), 10 mM NaF, 20 mM β‐glycerophosphate, and benzonase (Merck). .. After removal of insoluble materials by high‐speed centrifugation, lysates were precleared with GammaBind G Sepharose (17‐0885‐02, GE Healthcare).

Protein Extraction:

Article Title: Evaluation of Follicular Synchronization Caused by Estrogen Administration and Its Reproductive Outcome
Article Snippet: .. Western Blotting Frozen ovarian tissues were thawed in ice-cold protein extraction buffer (EB: 50 mmol/L HEPES (pH 7.5), 100mmol/L NaCl, 10 mmol/L MgCl2, 25 mmol/L β-glycerophosphate (Merck), 1mmol/L Na3VO4, 50 mmol/L NaF, 1mmol/L EDTA, 0.5 mmol/L EGTA; 10 mg/mL each of soybean trypsin inhibitor (Sigma), leupeptin and aprotinin (Amresco); and 1 mmol/L PMSF (Sigma)), then homogenized by the glass dounce homogenizer. ..

other:

Article Title: Comparison of Fecal Coliform Agar and Violet Red Bile Lactose Agar for Fecal Coliform Enumeration in Foods
Article Snippet: Solution B contained calcium lactate pentahydrate (14 g) (Merck) and β-glycerophosphate (1 g) (disodium salt; Merck).

Lysis:

Article Title: ErbB2 regulates autophagic flux to modulate the proteostasis of APP-CTFs in Alzheimer’s disease
Article Snippet: .. PBS-washed cells were resuspended in a lysis buffer containing 1% Triton X-100, 50 mM Tris⋅HCl (pH 7.5), 150 mM NaCl, 25 μM β-glycerophosphate, 1 mM Na3 VO4 , and protease inhibitor mixture set V (Merck). .. Following the removal of cell debris by centrifugation, clarified lysates were processed for immunoprecipitation, Western blotting, or quantification of protein content.

Western Blot:

Article Title: Upregulation of KLHDC4 Predicts a Poor Prognosis in Human Nasopharyngeal Carcinoma
Article Snippet: .. Western blotting Cells were lysed in NETN buffer (20 mM Tris-HCl at pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40) containing 50 mM β-glycerophosphate (14405, Merck), 1 μg/ml pepstatin A (P5318, Sigma-Aldrich) and 10 μM leupeptin (L2884, Sigma-Aldrich). .. The lysate protein concentration was measured using the BCA protein assay kit (Pierce); after normalization to equal amounts, proteins were separated by 12% SDS-PAGE, transferred to PVDF membranes and probed with the indicated primary antibodies.

Article Title: Evaluation of Follicular Synchronization Caused by Estrogen Administration and Its Reproductive Outcome
Article Snippet: .. Western Blotting Frozen ovarian tissues were thawed in ice-cold protein extraction buffer (EB: 50 mmol/L HEPES (pH 7.5), 100mmol/L NaCl, 10 mmol/L MgCl2, 25 mmol/L β-glycerophosphate (Merck), 1mmol/L Na3VO4, 50 mmol/L NaF, 1mmol/L EDTA, 0.5 mmol/L EGTA; 10 mg/mL each of soybean trypsin inhibitor (Sigma), leupeptin and aprotinin (Amresco); and 1 mmol/L PMSF (Sigma)), then homogenized by the glass dounce homogenizer. ..

Chloramphenicol Acetyltransferase Assay:

Article Title: Over-expression of Sox2 in C3H10T1/2 cells inhibits osteoblast differentiation through Wnt and MAPK signalling pathways
Article Snippet: .. The medium was changed to osteogenic differentiation medium the next day, which was supplemented with 10 mM β-glycerophosphate (Cat.No 35675, Merck, USA), 50 μg/ml of L-ascorbic acid (Cat.No A7506, Sigma-Aldrich, Switzerland) and 10-7 M dexamethasone (Cat.No D1756, Sigma-Aldrich, Switzerland). .. Cells were lysed with lysis buffer (Beyotime, P0013B) containing PMSF.

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  • 92
    Merck & Co beta glycerophosphate
    Beta Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/beta glycerophosphate/product/Merck & Co
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    beta glycerophosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Merck & Co phosphatase inhibitor cocktail
    Phosphatase Inhibitor Cocktail, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktail/product/Merck & Co
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktail - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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