Structured Review

Fisher Scientific β glycerophosphate
β Glycerophosphate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β glycerophosphate/product/Fisher Scientific
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
β glycerophosphate - by Bioz Stars, 2020-09
92/100 stars

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Modification:

Article Title: Atypical Mesenchymal Stromal Cell Responses to Topographic Modifications of Titanium Biomaterials Indicate Cytoskeletal- and Genetic Plasticity-Based Heterogeneity of Cells
Article Snippet: .. Osteogenic Differentiation Osteogenic differentiation was performed by culturing MSCs in 24-well plates for 7 days with osteogenic inductive medium (OM), comprising Dulbecco's modified Eagle's medium low glucose (Gibco) supplemented with 10% lot selected fetal calf serum (Gibco, UK), 1% antibiotics and 0.1% fungicide (Gibco, UK), 10 nM dexamethasone (water soluble; Sigma-Aldrich, UK), 10 mM β -glycerophosphate (Fisher Scientific, UK), and 50 μ M ascorbate-2-phosphate (Fluka, UK). .. Samples were washed twice with PBS and fixed in 10% formalin for 10-15 min before being stained with a 2% Alizarin Red S (Sigma-Aldrich, UK) solution in deionised water (pH 4.1-4.3) for 10 min.

Lysis:

Article Title: Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer
Article Snippet: .. Immunoblotting Cells were lysed in ice-cold lysis buffer (150 mM NaCl, 20 mM Tris [pH 7.5], 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pryophosphate, 1 mM β-glycerophosphate, 1 mM sodium vanadate, and one tablet of Pierce Protease Inhibitor Tablets, EDTA Free [Fisher Scientific-A32965] per 10 mL). .. Protein content was measured using Pierce BCA Protein Assay Kit (Life Technologies-23227), and 15–50 μg protein was resolved on 8–15% protein gels using SDS-PAGE and transferred onto PVDF membranes (EMD MIllipore-IPVH00010).

Article Title: Where It's at Really Matters: In Situ In Vivo Vascular Endothelial Growth Factor Spatially Correlates with Electron Paramagnetic Resonance pO2 Images in Tumors of Living Mice
Article Snippet: .. VEGF Determination in Tumor Biopsy Biopsies were weighed, homogenized, and transferred into a lysis buffer containing 50 mM Tris pH 7.4, 3 mM EDTA, 20 mM β-glycerophosphate, and proteases inhibitors cocktail (chemicals were from either Fisher Scientific, Pittsburgh, PA, USA, or Sigma Aldrich, St. Louis, MO, USA). .. After three freeze–thaw cycles to break the cell membranes, the homogenates were centrifuged for 5 min at 5,000×g (Eppendorf centrifuge 5,415°C), 4°C and supernatants were collected.

Protease Inhibitor:

Article Title: Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer
Article Snippet: .. Immunoblotting Cells were lysed in ice-cold lysis buffer (150 mM NaCl, 20 mM Tris [pH 7.5], 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pryophosphate, 1 mM β-glycerophosphate, 1 mM sodium vanadate, and one tablet of Pierce Protease Inhibitor Tablets, EDTA Free [Fisher Scientific-A32965] per 10 mL). .. Protein content was measured using Pierce BCA Protein Assay Kit (Life Technologies-23227), and 15–50 μg protein was resolved on 8–15% protein gels using SDS-PAGE and transferred onto PVDF membranes (EMD MIllipore-IPVH00010).

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    Fisher Scientific β glycerophosphate
    β Glycerophosphate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Fisher Scientific
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2020-09
    92/100 stars
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