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Beijing Solarbio Science β glycerophosphate
β Glycerophosphate, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β glycerophosphate/product/Beijing Solarbio Science
Average 90 stars, based on 4 article reviews
Price from $9.99 to $1999.99
β glycerophosphate - by Bioz Stars, 2020-09
90/100 stars

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Related Articles

Centrifugation:

Article Title: Synthesis of Chalcone Derivatives: Inducing Apoptosis of HepG2 Cells via Regulating Reactive Oxygen Species and Mitochondrial Pathway
Article Snippet: .. The Expression of Apoptotic Protein in Hepg2 Cells HepG2 cells (3 × 105 cells/well) were seeded into a six-well plate overnight for adherence, and then treated with a14 (0, 30, 40, 50, 60, and 70 µM) for 48 h. After the cells were collected by centrifugation, they were lysed with the radio immunoprecipitation assay (RIPA) buffer (high) containing 50 mM Tris (pH = 7.4), 150 mM NaCl, 1% TritonX-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM Na3 VO4 , and 0.5 ug/ml leupeptin (Beijing Solarbio Science & Technology Co., Ltd.).The extract was then centrifuged at 12,000 rpm for 30 min and the total protein concentration was quantified using the BCA kit (Beijing Solarbio Science & Technology Co., Ltd.). .. The protein extracts were mixed with the SDS-PAGE protein loading buffer (Beijing Solarbio Science & Technology Co., Ltd.) and then boiled in 100°C water bath for 10 min.

Radio Immunoprecipitation:

Article Title: Synthesis of Chalcone Derivatives: Inducing Apoptosis of HepG2 Cells via Regulating Reactive Oxygen Species and Mitochondrial Pathway
Article Snippet: .. The Expression of Apoptotic Protein in Hepg2 Cells HepG2 cells (3 × 105 cells/well) were seeded into a six-well plate overnight for adherence, and then treated with a14 (0, 30, 40, 50, 60, and 70 µM) for 48 h. After the cells were collected by centrifugation, they were lysed with the radio immunoprecipitation assay (RIPA) buffer (high) containing 50 mM Tris (pH = 7.4), 150 mM NaCl, 1% TritonX-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM Na3 VO4 , and 0.5 ug/ml leupeptin (Beijing Solarbio Science & Technology Co., Ltd.).The extract was then centrifuged at 12,000 rpm for 30 min and the total protein concentration was quantified using the BCA kit (Beijing Solarbio Science & Technology Co., Ltd.). .. The protein extracts were mixed with the SDS-PAGE protein loading buffer (Beijing Solarbio Science & Technology Co., Ltd.) and then boiled in 100°C water bath for 10 min.

Protein Concentration:

Article Title: Synthesis of Chalcone Derivatives: Inducing Apoptosis of HepG2 Cells via Regulating Reactive Oxygen Species and Mitochondrial Pathway
Article Snippet: .. The Expression of Apoptotic Protein in Hepg2 Cells HepG2 cells (3 × 105 cells/well) were seeded into a six-well plate overnight for adherence, and then treated with a14 (0, 30, 40, 50, 60, and 70 µM) for 48 h. After the cells were collected by centrifugation, they were lysed with the radio immunoprecipitation assay (RIPA) buffer (high) containing 50 mM Tris (pH = 7.4), 150 mM NaCl, 1% TritonX-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM Na3 VO4 , and 0.5 ug/ml leupeptin (Beijing Solarbio Science & Technology Co., Ltd.).The extract was then centrifuged at 12,000 rpm for 30 min and the total protein concentration was quantified using the BCA kit (Beijing Solarbio Science & Technology Co., Ltd.). .. The protein extracts were mixed with the SDS-PAGE protein loading buffer (Beijing Solarbio Science & Technology Co., Ltd.) and then boiled in 100°C water bath for 10 min.

Cell Culture:

Article Title: Downregulated microRNA-23b promotes BMP9-mediated osteogenesis in C2C12 myoblast cells by targeting Runx2
Article Snippet: .. Briefly, cells were cultured in the presence of ascorbic acid (50 µ g/ml; Beijing Solarbio Science and Technology Co., Ltd., Beijing, China) and β-glycerophosphate (10 mmol/l; Beijing Solarbio Science and Technology Co., Ltd.). .. Cells were fixed with 0.05% (v/v) glutaraldehyde (Chongqing Chuandong Chemical Group Co., Ltd., Chongqing, China) at room temperature for 10 min.

Expressing:

Article Title: Synthesis of Chalcone Derivatives: Inducing Apoptosis of HepG2 Cells via Regulating Reactive Oxygen Species and Mitochondrial Pathway
Article Snippet: .. The Expression of Apoptotic Protein in Hepg2 Cells HepG2 cells (3 × 105 cells/well) were seeded into a six-well plate overnight for adherence, and then treated with a14 (0, 30, 40, 50, 60, and 70 µM) for 48 h. After the cells were collected by centrifugation, they were lysed with the radio immunoprecipitation assay (RIPA) buffer (high) containing 50 mM Tris (pH = 7.4), 150 mM NaCl, 1% TritonX-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM Na3 VO4 , and 0.5 ug/ml leupeptin (Beijing Solarbio Science & Technology Co., Ltd.).The extract was then centrifuged at 12,000 rpm for 30 min and the total protein concentration was quantified using the BCA kit (Beijing Solarbio Science & Technology Co., Ltd.). .. The protein extracts were mixed with the SDS-PAGE protein loading buffer (Beijing Solarbio Science & Technology Co., Ltd.) and then boiled in 100°C water bath for 10 min.

BIA-KA:

Article Title: Synthesis of Chalcone Derivatives: Inducing Apoptosis of HepG2 Cells via Regulating Reactive Oxygen Species and Mitochondrial Pathway
Article Snippet: .. The Expression of Apoptotic Protein in Hepg2 Cells HepG2 cells (3 × 105 cells/well) were seeded into a six-well plate overnight for adherence, and then treated with a14 (0, 30, 40, 50, 60, and 70 µM) for 48 h. After the cells were collected by centrifugation, they were lysed with the radio immunoprecipitation assay (RIPA) buffer (high) containing 50 mM Tris (pH = 7.4), 150 mM NaCl, 1% TritonX-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM Na3 VO4 , and 0.5 ug/ml leupeptin (Beijing Solarbio Science & Technology Co., Ltd.).The extract was then centrifuged at 12,000 rpm for 30 min and the total protein concentration was quantified using the BCA kit (Beijing Solarbio Science & Technology Co., Ltd.). .. The protein extracts were mixed with the SDS-PAGE protein loading buffer (Beijing Solarbio Science & Technology Co., Ltd.) and then boiled in 100°C water bath for 10 min.

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    Beijing Solarbio Science β glycerophosphate
    β Glycerophosphate, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Beijing Solarbio Science
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2020-09
    90/100 stars
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