α tetralone  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Name:
    alpha Tetralone
    Description:

    Catalog Number:
    87661
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore α tetralone
    alpha Tetralone

    https://www.bioz.com/result/α tetralone/product/Millipore
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    α tetralone - by Bioz Stars, 2020-09
    91/100 stars

    Images

    1) Product Images from "Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum"

    Article Title: Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063828

    Binding interaction of α-tetralone in the substrate binding site of PyAeADHII/NADPH predicted by AutoDock. a) Binding mode 1, α-tetralone was positioned on top of the nicotinamide ring as a stacking interaction, and the oxygen atom formed a H-bonding interaction with the side chain of residue Asn-39 (3.5 Å). b) α-tetralone again forms stacking interaction with nicotinamide ring, but the oxygen atom was orientated towards residue Arg-88 forming a H-bonding interaction (3.0 Å). PyAeADHII is shown as a cartoon (green) and residues in the substrate binding site are shown as sticks (carbon colored in yellow, nitrogen in blue, oxygen in red). NADPH (carbons are in grey) and the substrate α-tetralone (carbon are in orange) are shown as sticks.
    Figure Legend Snippet: Binding interaction of α-tetralone in the substrate binding site of PyAeADHII/NADPH predicted by AutoDock. a) Binding mode 1, α-tetralone was positioned on top of the nicotinamide ring as a stacking interaction, and the oxygen atom formed a H-bonding interaction with the side chain of residue Asn-39 (3.5 Å). b) α-tetralone again forms stacking interaction with nicotinamide ring, but the oxygen atom was orientated towards residue Arg-88 forming a H-bonding interaction (3.0 Å). PyAeADHII is shown as a cartoon (green) and residues in the substrate binding site are shown as sticks (carbon colored in yellow, nitrogen in blue, oxygen in red). NADPH (carbons are in grey) and the substrate α-tetralone (carbon are in orange) are shown as sticks.

    Techniques Used: Binding Assay

    Inhibition of PyAeADHII at 70°C by flunarizine dihydrochloride. The assay was performed with 260 nM PyAeADHII in 50 mM NaPO4 pH 7.5, 3 mM α-tetralone, 0.3 mM NADPH in the presence of 80–1200 µM flunarizine dihydrochloride in DMSO/isopropanol. a) Absorbance of NADPH (340 nm) was measured over time at different flunarizine concentrations. b) The rate of NADPH conversion (Δa.u. = absorbance unit change/minute) versus flunarizine concentration.
    Figure Legend Snippet: Inhibition of PyAeADHII at 70°C by flunarizine dihydrochloride. The assay was performed with 260 nM PyAeADHII in 50 mM NaPO4 pH 7.5, 3 mM α-tetralone, 0.3 mM NADPH in the presence of 80–1200 µM flunarizine dihydrochloride in DMSO/isopropanol. a) Absorbance of NADPH (340 nm) was measured over time at different flunarizine concentrations. b) The rate of NADPH conversion (Δa.u. = absorbance unit change/minute) versus flunarizine concentration.

    Techniques Used: Inhibition, Concentration Assay

    2) Product Images from "Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum"

    Article Title: Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063828

    Binding interaction of α-tetralone in the substrate binding site of PyAeADHII/NADPH predicted by AutoDock. a) Binding mode 1, α-tetralone was positioned on top of the nicotinamide ring as a stacking interaction, and the oxygen atom formed a H-bonding interaction with the side chain of residue Asn-39 (3.5 Å). b) α-tetralone again forms stacking interaction with nicotinamide ring, but the oxygen atom was orientated towards residue Arg-88 forming a H-bonding interaction (3.0 Å). PyAeADHII is shown as a cartoon (green) and residues in the substrate binding site are shown as sticks (carbon colored in yellow, nitrogen in blue, oxygen in red). NADPH (carbons are in grey) and the substrate α-tetralone (carbon are in orange) are shown as sticks.
    Figure Legend Snippet: Binding interaction of α-tetralone in the substrate binding site of PyAeADHII/NADPH predicted by AutoDock. a) Binding mode 1, α-tetralone was positioned on top of the nicotinamide ring as a stacking interaction, and the oxygen atom formed a H-bonding interaction with the side chain of residue Asn-39 (3.5 Å). b) α-tetralone again forms stacking interaction with nicotinamide ring, but the oxygen atom was orientated towards residue Arg-88 forming a H-bonding interaction (3.0 Å). PyAeADHII is shown as a cartoon (green) and residues in the substrate binding site are shown as sticks (carbon colored in yellow, nitrogen in blue, oxygen in red). NADPH (carbons are in grey) and the substrate α-tetralone (carbon are in orange) are shown as sticks.

    Techniques Used: Binding Assay

    Inhibition of PyAeADHII at 70°C by flunarizine dihydrochloride. The assay was performed with 260 nM PyAeADHII in 50 mM NaPO4 pH 7.5, 3 mM α-tetralone, 0.3 mM NADPH in the presence of 80–1200 µM flunarizine dihydrochloride in DMSO/isopropanol. a) Absorbance of NADPH (340 nm) was measured over time at different flunarizine concentrations. b) The rate of NADPH conversion (Δa.u. = absorbance unit change/minute) versus flunarizine concentration.
    Figure Legend Snippet: Inhibition of PyAeADHII at 70°C by flunarizine dihydrochloride. The assay was performed with 260 nM PyAeADHII in 50 mM NaPO4 pH 7.5, 3 mM α-tetralone, 0.3 mM NADPH in the presence of 80–1200 µM flunarizine dihydrochloride in DMSO/isopropanol. a) Absorbance of NADPH (340 nm) was measured over time at different flunarizine concentrations. b) The rate of NADPH conversion (Δa.u. = absorbance unit change/minute) versus flunarizine concentration.

    Techniques Used: Inhibition, Concentration Assay

    Related Articles

    Concentration Assay:

    Article Title: Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum
    Article Snippet: .. The standard assay for the reduction reaction of α-tetralone was performed by adding 2.6 μM final concentration of the enzyme to a preheated assay mixture containing 3 mM substrate (α-tetralone; Sigma-Aldrich #T19003, Lot S28914 or from Tokyo Chemical Industry #T0134), 0.3 mM NADPH (Sigma-Aldrich #N5130) in 50 mM sodium phosphate (pH 7.5) . ..

    Centrifugation:

    Article Title: Alkylated Piperazines and Piperazine-Azole Hybrids as Antifungal Agents
    Article Snippet: .. Cells were harvested by centrifugation (5000 rpm) for 10 min at room temperature, and cell pellets were saponified at 80 °C for 2 h with 3 mL of MeOH, 2 mL of pyrogallol dissolved in MeOH (0.5%, w/v) (CAS 87-66-1, Sigma-Aldrich, St. Louis, MO), and 2 mL of KOH (60%, w/v). ..

    Article Title: New Application of Neomycin B–Bisbenzimidazole Hybrids as Antifungal Agents
    Article Snippet: .. The cells were harvested by centrifugation (5000 rpm) for 10 min at room temperature, and the cell pellets were saponified at 80 °C for 2 h with 3 mL of MeOH, 2 mL of pyrogallol dissolved in MeOH (0.5%, wt/v) (CAS # 87-66-1, Sigma-Aldrich Chemical Co., St. Louis, Mo.), and 2 mL of potassium hydroxide (60%, wt/v). ..

    other:

    Article Title: New potent and selective cytochrome P450 2B6 (CYP2B6) inhibitors based on three-dimensional quantitative structure-activity relationship (3D-QSAR) analysis
    Article Snippet: 1-naphthol and 4-methoxybenzaldehyde were purchased from Sigma Chemical Co. (St Louis, MO, USA). γ -Heptalactone, γ -dodecanolactone, γ -caprolactone, ɛ -caprolactone, δ -hexalactone and α -tetralone were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Activity Assay:

    Article Title: Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum
    Article Snippet: .. Interestingly, we found that some samples of α-tetralone purchased from Sigma-Aldrich and Tokyo Chemical Industry appeared to contain small amounts of contaminating compounds (as determined by mass spectrometry) that effectively inhibited PyAeADHII activity. .. These contaminants needed to be removed by HPLC in order to isolate pure α-tetralone.

    Mass Spectrometry:

    Article Title: Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum
    Article Snippet: .. Interestingly, we found that some samples of α-tetralone purchased from Sigma-Aldrich and Tokyo Chemical Industry appeared to contain small amounts of contaminating compounds (as determined by mass spectrometry) that effectively inhibited PyAeADHII activity. .. These contaminants needed to be removed by HPLC in order to isolate pure α-tetralone.