α ha immune complexes  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Millipore α ha immune complexes
    Validation of subcellular localization predictions using <t>α-HA</t> immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.
    α Ha Immune Complexes, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α ha immune complexes/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α ha immune complexes - by Bioz Stars, 2020-07
    91/100 stars

    Images

    1) Product Images from "Architecture of the human interactome defines protein communities and disease networks"

    Article Title: Architecture of the human interactome defines protein communities and disease networks

    Journal: Nature

    doi: 10.1038/nature22366

    Validation of subcellular localization predictions using α-HA immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.
    Figure Legend Snippet: Validation of subcellular localization predictions using α-HA immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.

    Techniques Used: Immunofluorescence, Infection, Staining, Confocal Microscopy

    BioPlex network coverage and validation of interactions for a set of poorly studied proteins in BioPlex 2.0 using HCT116 cells a , BioPlex network coverage of selected protein classes. Light shades represent total proteins, while dark shades represent baits targeted for AP-MS. BioPlex 1.0 is depicted in blue shades while BioPlex 2.0 is highlighted in red. b – m , The indicated bait proteins (teal) were expressed in HCT116 cells and α-HA immune complexes analyzed by mass spectrometry. HCIPs were determined using CompPASS-Plus . Interactions observed in both HCT116 and HEK293T cells are indicated with blue edges and nodes. Interactions seen in HEK293T but not HCT116 are shown in grey edges and nodes. b , TMEM111; c , ZNHIT3; d , RMND5A; e , SMTNL2; f , FBXO28; g , C3orf75; h , c9orf41; i , MPP2; j , ZNF219; k , ZNF483; l , WDR37; m , LRCH3.
    Figure Legend Snippet: BioPlex network coverage and validation of interactions for a set of poorly studied proteins in BioPlex 2.0 using HCT116 cells a , BioPlex network coverage of selected protein classes. Light shades represent total proteins, while dark shades represent baits targeted for AP-MS. BioPlex 1.0 is depicted in blue shades while BioPlex 2.0 is highlighted in red. b – m , The indicated bait proteins (teal) were expressed in HCT116 cells and α-HA immune complexes analyzed by mass spectrometry. HCIPs were determined using CompPASS-Plus . Interactions observed in both HCT116 and HEK293T cells are indicated with blue edges and nodes. Interactions seen in HEK293T but not HCT116 are shown in grey edges and nodes. b , TMEM111; c , ZNHIT3; d , RMND5A; e , SMTNL2; f , FBXO28; g , C3orf75; h , c9orf41; i , MPP2; j , ZNF219; k , ZNF483; l , WDR37; m , LRCH3.

    Techniques Used: Mass Spectrometry

    Related Articles

    Affinity Purification:

    Article Title: Architecture of the human interactome defines protein communities and disease networks
    Article Snippet: .. Affinity purification was performed essentially as described previously , but eluted α-HA immune complexes (Sigma-Aldrich, clone HA-7) were analyzed in two ways. .. First, immune complexes for PDLIM7, MAGI1, YAP1, WWC1, NF2, and MPP5 (Replicate 1) were subjected to LC-MS/MS analysis on an LTQ-Velos instrument and HCIPs identified using CompPASS in combination with a false positive background data set derived in MCF10A cells .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore mouse β actin antibody
    Mouse β Actin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse β actin antibody/product/Millipore
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    mouse β actin antibody - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results