mtz  (ATCC)


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  • 91
    Name:
    CT26 WT
    Description:

    Catalog Number:
    crl-2638
    Price:
    None
    Applications:
    The cell line can be used with CT26.CL25 (ATCC CRL-2639) as a model for testing immunotherapy protocols and in studies on the host immune response.
    Host:
    Mus musculus, mouse
    Cell Type:
    fibroblast
    Buy from Supplier


    Structured Review

    ATCC mtz
    F. <t>nucleatum</t> ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole <t>(726+MTZ.),</t> metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    https://www.bioz.com/result/mtz/product/ATCC
    Average 91 stars, based on 199 article reviews
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    mtz - by Bioz Stars, 2020-09
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    Images

    1) Product Images from "Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression"

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    Journal: Nature Communications

    doi: 10.1038/s41467-020-16967-2

    F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.
    Figure Legend Snippet: F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    Techniques Used: Injection, Mouse Assay, One-tailed Test, MANN-WHITNEY, Fluorescence, Expressing

    2) Product Images from "Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System"

    Article Title: Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2020.00400

    The bloodstream is an efficient route of oral F. nucleatum for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p
    Figure Legend Snippet: The bloodstream is an efficient route of oral F. nucleatum for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p

    Techniques Used: Mouse Assay, Staining, Labeling, Binding Assay, Mutagenesis, Negative Control, In Vivo, Tumor Implantation

    Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p
    Figure Legend Snippet: Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p

    Techniques Used: Stable Transfection, Transfection, Luciferase, Mouse Assay

    Efficiency of CRC colonization by hematogenous fusobacteria is dose-dependent. (A) Relative fusobacterial gDNA abundance (2 −Δ Ct ), and proportion CRC colonization (B) in tumor samples from MC38 transplanted mice intravenously inoculated daily three times with 5 × 10 3 -1 × 10 4 , 5 × 10 4 -1 × 10 5 or with 5 × 10 6 -1 × 10 7 F. nucleatum JAoral001. Each symbol represents one mouse. Bar show median. * p
    Figure Legend Snippet: Efficiency of CRC colonization by hematogenous fusobacteria is dose-dependent. (A) Relative fusobacterial gDNA abundance (2 −Δ Ct ), and proportion CRC colonization (B) in tumor samples from MC38 transplanted mice intravenously inoculated daily three times with 5 × 10 3 -1 × 10 4 , 5 × 10 4 -1 × 10 5 or with 5 × 10 6 -1 × 10 7 F. nucleatum JAoral001. Each symbol represents one mouse. Bar show median. * p

    Techniques Used: Mouse Assay

    3) Product Images from "Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System"

    Article Title: Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2020.00400

    Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p
    Figure Legend Snippet: Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p

    Techniques Used: Stable Transfection, Transfection, Luciferase, Mouse Assay

    4) Product Images from "Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression"

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    Journal: Nature Communications

    doi: 10.1038/s41467-020-16967-2

    F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p
    Figure Legend Snippet: F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p

    Techniques Used: Injection, Fluorescence, Binding Assay, Labeling, Staining, One-tailed Test, Mouse Assay, MANN-WHITNEY, Mutagenesis

    5) Product Images from "Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression"

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    Journal: Nature Communications

    doi: 10.1038/s41467-020-16967-2

    F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.
    Figure Legend Snippet: F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    Techniques Used: Injection, Mouse Assay, One-tailed Test, MANN-WHITNEY, Fluorescence, Expressing

    F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p
    Figure Legend Snippet: F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p

    Techniques Used: Injection, Fluorescence, Binding Assay, Labeling, Staining, One-tailed Test, Mouse Assay, MANN-WHITNEY, Mutagenesis

    Attachment of F. nucleatum ATCC 23726 to breast cancer is Fap2-mediated and Gal-GalNAc dependent. a Representative images (left panels, scale bar 20 µm) and quantitative analysis (Fn/mm 2 ) (right panel) of binding of Cy3-labeled (white) WT F. nucleatum ATCC 23726 (726) and of its Cy5-labeled (red) Fap2-inactivated isogenic mutant K50, to Hoechst-stained (blue) human breast cancer TMAs (HBre-Duc060CS-01 and BR1006). Results in the right panel are classified by tissue type (normal n = 35, benign n = 7, malignant n = 64) and bacterial strain (K50, 726). Each core is measured twice, once for each of the strains in the appropriate tissue type (as demonstrated in the pictures). When an individual contributed a single core to the experiment, the two observations are shown as circles (●); when she contributed two cores, these were to the normal type and the malignant type, and the four observations are depicted as plus signs (+). The six classes were compared against each other in pairs: the exact two-tailed Wilcoxon test was used for pairs that were from the same core, the exact two-tailed Mann–Whitney test for independent samples. Where the same hypothesis was tested on more than one pair, and the individual pairs were mutually independent, the corresponding p -values were combined by Fisher’s method. Wherever the pairs were not independent, the multiple comparisons were adjusted for using the Holm modification of the Bonferroni correction. **** p
    Figure Legend Snippet: Attachment of F. nucleatum ATCC 23726 to breast cancer is Fap2-mediated and Gal-GalNAc dependent. a Representative images (left panels, scale bar 20 µm) and quantitative analysis (Fn/mm 2 ) (right panel) of binding of Cy3-labeled (white) WT F. nucleatum ATCC 23726 (726) and of its Cy5-labeled (red) Fap2-inactivated isogenic mutant K50, to Hoechst-stained (blue) human breast cancer TMAs (HBre-Duc060CS-01 and BR1006). Results in the right panel are classified by tissue type (normal n = 35, benign n = 7, malignant n = 64) and bacterial strain (K50, 726). Each core is measured twice, once for each of the strains in the appropriate tissue type (as demonstrated in the pictures). When an individual contributed a single core to the experiment, the two observations are shown as circles (●); when she contributed two cores, these were to the normal type and the malignant type, and the four observations are depicted as plus signs (+). The six classes were compared against each other in pairs: the exact two-tailed Wilcoxon test was used for pairs that were from the same core, the exact two-tailed Mann–Whitney test for independent samples. Where the same hypothesis was tested on more than one pair, and the individual pairs were mutually independent, the corresponding p -values were combined by Fisher’s method. Wherever the pairs were not independent, the multiple comparisons were adjusted for using the Holm modification of the Bonferroni correction. **** p

    Techniques Used: Binding Assay, Labeling, Mutagenesis, Staining, Two Tailed Test, MANN-WHITNEY, Modification

    6) Product Images from "Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System"

    Article Title: Colon Cancer-Associated Fusobacterium nucleatum May Originate From the Oral Cavity and Reach Colon Tumors via the Circulatory System

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2020.00400

    The bloodstream is an efficient route of oral F. nucleatum for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p
    Figure Legend Snippet: The bloodstream is an efficient route of oral F. nucleatum for CRC enrichment. (A) MC38 mice CRC cells display high Gal-GalNAc levels. Representative images of MC38 cells and of human adenocarcinoma HT-29 cells unstained (left panels), or stained with FITC-labeled Gal-GalNAc-specific PNA (green, right panels). (B) Hemagglutination demonstrating Fap2-dependent Gal-GalNAc binding by matching oral and CRC isolates O2/T2 with Oral003/CRC003 and JAoral001/JAcrc001 in the absence (left) and in the presence (right) of 25 mM GalNAc. The F. nucleatum ATCC 23726 Fap2 inactivated mutant K50 was used for negative control. Non-hemagglutinated erythrocytes settle in the bottom of the round bottom well. (C) in vivo experimental scheme of the orthotopic rectal MC38-luc mouse CRC model. At day 9 post-tumor implantation mice were randomized to an oral or intravenous inoculation group. Oral inoculations were performed on day 9, 12, and 15. A single intravenous inoculation was performed on day 15. (D,E) CRC colonization by hematogenously or orally administered fusobacteria. (D) Abundance (CFU/gr tissue) and relative fusobacterial gDNA abundance (2 −Δ Ct ) (E) in tumor (T) samples and in adjacent normal (N) colon samples from MC38 transplanted mice inoculated once with 5 × 10 6 -1 × 10 7 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum O2 or Oral003. **** p

    Techniques Used: Mouse Assay, Staining, Labeling, Binding Assay, Mutagenesis, Negative Control, In Vivo, Tumor Implantation

    Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p
    Figure Legend Snippet: Kinetics of F. nucleatum ATCC 23726 enrichment in the CT-26 orthotopic model. (A) Detection of implanted CT-26 cells stably transfected with the luciferase (luc) gene under the mucosa of the distal rectum of C57BL/6 wild-type mice. (B) Relative fusobacterial gDNA abundance (2 −Δ Ct ) in tumor samples and in adjacent normal colon samples from CT26 transplanted BALB/cJ mice inoculated once with 5 × 10 7 -1 × 10 8 intravenously (IV) or three times by oral gavage (Gavage) with 10 ~10 F. nucleatum ATCC 23726. * p

    Techniques Used: Stable Transfection, Transfection, Luciferase, Mouse Assay

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    other:

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    Cell Culture:

    Article Title: Bacterial ghosts as adjuvant to oxaliplatin chemotherapy in colorectal carcinomatosis
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    Article Title: microRNA-451a regulates colorectal cancer proliferation in response to radiation
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    Mouse Assay:

    Article Title: aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant
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    Article Title: Instant immunity through chemically programmable vaccination and covalent self-assembly
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    ATCC × 107 f nucleatum atcc 23726
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    ATCC fusobacterium nucleatum subsp nucleatum vpi 4351 1210
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