Journal: Journal of Ginseng Research

Article Title: Korean Red Ginseng and Korean black ginseng extracts, JP5 and BG1, prevent hepatic oxidative stress and inflammation induced by environmental heat stress

doi: 10.1016/j.jgr.2018.12.005

Figure Lengend Snippet: Expression of inflammatory proteins in liver tissue and HepG2 cells. (A) Expression of proteins related to inflammation, including p-NF-κB, p-JNK, JNK, and TNF-α analyzed by Western blot. (B and D) Expression of proteins related to the NLRP3 inflammasome and apoptosis, including NLRP3, caspase-1, IL-1β, and BCL2, in (B) liver tissue and (D) HepG2 cells. (C)The effects of Rg5 and heat stress on expression of inflammatory factors p-NF-κB and TNF-α were confirmed in HepG2 cells. GAPDH was used as a loading control. TNF-α, tumor necrosis factor-alpha; RT, room temperature; HS, heat stress.

Article Snippet: Antibodies against p-NF-κB, TNF-α, NLRP3, caspase-1, IL-1β, B-cell lymphoma 2 (BCL2), and JNK were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Expressing, Western Blot