c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 9
    Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 9
    Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 9/product/Cell Signaling Technology Inc
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    anti cleaved caspase9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cleaved caspase9
    Mfn2 siRNA inhibited endoplasmic reticulum- (ER-) mitochondria-dependent apoptosis. (a) Cell apoptosis was evaluated by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. (b) HL-1 cells under TM stimulated for 24 hours. Mfn siRNA inhibited the Bax/Bcl-2 ratio and inhibited caspase3 and <t>caspase9</t> activation (c). Data are mean ± SEM, n = 4 independent experiments, ∗ p < 0.05, # p < 0.01, ANOVA with Bonferroni posttest.
    Anti Cleaved Caspase9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Hyperglycemia Induces Endoplasmic Reticulum Stress in Atrial Cardiomyocytes, and Mitofusin-2 Downregulation Prevents Mitochondrial Dysfunction and Subsequent Cell Death"

    Article Title: Hyperglycemia Induces Endoplasmic Reticulum Stress in Atrial Cardiomyocytes, and Mitofusin-2 Downregulation Prevents Mitochondrial Dysfunction and Subsequent Cell Death

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2020/6569728

    Mfn2 siRNA inhibited endoplasmic reticulum- (ER-) mitochondria-dependent apoptosis. (a) Cell apoptosis was evaluated by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. (b) HL-1 cells under TM stimulated for 24 hours. Mfn siRNA inhibited the Bax/Bcl-2 ratio and inhibited caspase3 and caspase9 activation (c). Data are mean ± SEM, n = 4 independent experiments, ∗ p < 0.05, # p < 0.01, ANOVA with Bonferroni posttest.
    Figure Legend Snippet: Mfn2 siRNA inhibited endoplasmic reticulum- (ER-) mitochondria-dependent apoptosis. (a) Cell apoptosis was evaluated by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. (b) HL-1 cells under TM stimulated for 24 hours. Mfn siRNA inhibited the Bax/Bcl-2 ratio and inhibited caspase3 and caspase9 activation (c). Data are mean ± SEM, n = 4 independent experiments, ∗ p < 0.05, # p < 0.01, ANOVA with Bonferroni posttest.

    Techniques Used: Flow Cytometry, Staining, Activation Assay

    anti cleaved caspase 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cleaved caspase 9
    Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 <t>and</t> <t>cleaved</t> <t>caspase-9.</t> *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis
    Anti Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Maresin-1 Inhibits Oxidative Stress and Inflammation and Promotes Apoptosis in a Mouse Model of Caerulein-Induced Acute Pancreatitis"

    Article Title: Maresin-1 Inhibits Oxidative Stress and Inflammation and Promotes Apoptosis in a Mouse Model of Caerulein-Induced Acute Pancreatitis

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.917380

    Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 and cleaved caspase-9. *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis
    Figure Legend Snippet: Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 and cleaved caspase-9. *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis

    Techniques Used: Expressing, Western Blot

    rabbit polyclonal anti cleaved caspase 9 asp353  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti cleaved caspase 9 asp353
    The specific SOD1 inhibition activates mitochondria-dependent apoptosis of cancer cells. (a, b) RT-qPCR (a) and western blotting (b) examination of p53 mRNA levels and protein content in 50 (RT-qPCR) or 0-100 μ M (western blotting) LD100-treated HeLa, DU145, and RWPE-1 cells. (c) The protein levels of p21 and p19 in HeLa and DU145 cells are evaluated by western blotting analysis, after the cells are incubated with varied concentrations of LD100 for 24 h. β -Actin is used as an internal control (b, c). (d) The protein levels of proto- <t>and</t> <t>cleaved</t> <t>caspase-9</t> and -3 in HeLa cells are evaluated by western blotting analysis, after HeLa cells are incubated with varied concentrations of LD100 for 24 h. (e) The time dependence of the protein levels of proto- and cleaved caspase-9 is evaluated by western blotting analysis in 50 μ M LD100-treated HeLa cells. (f, h) HeLa cells are incubated with varied concentrations of LD100 for 24 h prior to western blotting evaluation of Bcl-2 (f) and cleaved PARP1 (h) protein levels. (g) Rh123 fluorescence that is positively correlated with mitochondrial membrane potential is measured by flow cytometry in HeLa, DU145, and RWPE-1 cells after incubated with varied concentrations of LD100 for 24 h. (i) DNA fragmentation in HeLa cells is assayed using agarose gel electrophoresis after incubated with varied concentrations of LD100 for 24 h. (j) The peroxynitrite content in HeLa cells is presented by DHR123 fluorescence measured with flow cytometry after incubation for 24 h, respectively, with LD100 (0, 50, and 100 μ M) and L-NMMA (0, 1 mM), a cell membrane-permeable competitive NOS inhibitor. (k) After incubation for 24 h, respectively, with LD100 (0, 100 μ M) and L-NMMA (1 mM L-NMMA), flow cytometric analysis of HeLa cell apoptosis is carried out with Annexin V and propidium iodide staining. To obtain more significant results, 100 μ M LD100 was used here. The relative intensity of protein bands (means ± SD) in the western blotting is quantified by using the ImageJ software and normalized through the negative control, respectively (b–g). Representative results from three independent experiments are shown (b–g and k). Data are mean of triplicate samples ± SD (ns: not statistically significant; ∗ P < 0.05; unpaired Student's t -test), and all error bars are SD.
    Rabbit Polyclonal Anti Cleaved Caspase 9 Asp353, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network"

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2019/9706792

    The specific SOD1 inhibition activates mitochondria-dependent apoptosis of cancer cells. (a, b) RT-qPCR (a) and western blotting (b) examination of p53 mRNA levels and protein content in 50 (RT-qPCR) or 0-100 μ M (western blotting) LD100-treated HeLa, DU145, and RWPE-1 cells. (c) The protein levels of p21 and p19 in HeLa and DU145 cells are evaluated by western blotting analysis, after the cells are incubated with varied concentrations of LD100 for 24 h. β -Actin is used as an internal control (b, c). (d) The protein levels of proto- and cleaved caspase-9 and -3 in HeLa cells are evaluated by western blotting analysis, after HeLa cells are incubated with varied concentrations of LD100 for 24 h. (e) The time dependence of the protein levels of proto- and cleaved caspase-9 is evaluated by western blotting analysis in 50 μ M LD100-treated HeLa cells. (f, h) HeLa cells are incubated with varied concentrations of LD100 for 24 h prior to western blotting evaluation of Bcl-2 (f) and cleaved PARP1 (h) protein levels. (g) Rh123 fluorescence that is positively correlated with mitochondrial membrane potential is measured by flow cytometry in HeLa, DU145, and RWPE-1 cells after incubated with varied concentrations of LD100 for 24 h. (i) DNA fragmentation in HeLa cells is assayed using agarose gel electrophoresis after incubated with varied concentrations of LD100 for 24 h. (j) The peroxynitrite content in HeLa cells is presented by DHR123 fluorescence measured with flow cytometry after incubation for 24 h, respectively, with LD100 (0, 50, and 100 μ M) and L-NMMA (0, 1 mM), a cell membrane-permeable competitive NOS inhibitor. (k) After incubation for 24 h, respectively, with LD100 (0, 100 μ M) and L-NMMA (1 mM L-NMMA), flow cytometric analysis of HeLa cell apoptosis is carried out with Annexin V and propidium iodide staining. To obtain more significant results, 100 μ M LD100 was used here. The relative intensity of protein bands (means ± SD) in the western blotting is quantified by using the ImageJ software and normalized through the negative control, respectively (b–g). Representative results from three independent experiments are shown (b–g and k). Data are mean of triplicate samples ± SD (ns: not statistically significant; ∗ P < 0.05; unpaired Student's t -test), and all error bars are SD.
    Figure Legend Snippet: The specific SOD1 inhibition activates mitochondria-dependent apoptosis of cancer cells. (a, b) RT-qPCR (a) and western blotting (b) examination of p53 mRNA levels and protein content in 50 (RT-qPCR) or 0-100 μ M (western blotting) LD100-treated HeLa, DU145, and RWPE-1 cells. (c) The protein levels of p21 and p19 in HeLa and DU145 cells are evaluated by western blotting analysis, after the cells are incubated with varied concentrations of LD100 for 24 h. β -Actin is used as an internal control (b, c). (d) The protein levels of proto- and cleaved caspase-9 and -3 in HeLa cells are evaluated by western blotting analysis, after HeLa cells are incubated with varied concentrations of LD100 for 24 h. (e) The time dependence of the protein levels of proto- and cleaved caspase-9 is evaluated by western blotting analysis in 50 μ M LD100-treated HeLa cells. (f, h) HeLa cells are incubated with varied concentrations of LD100 for 24 h prior to western blotting evaluation of Bcl-2 (f) and cleaved PARP1 (h) protein levels. (g) Rh123 fluorescence that is positively correlated with mitochondrial membrane potential is measured by flow cytometry in HeLa, DU145, and RWPE-1 cells after incubated with varied concentrations of LD100 for 24 h. (i) DNA fragmentation in HeLa cells is assayed using agarose gel electrophoresis after incubated with varied concentrations of LD100 for 24 h. (j) The peroxynitrite content in HeLa cells is presented by DHR123 fluorescence measured with flow cytometry after incubation for 24 h, respectively, with LD100 (0, 50, and 100 μ M) and L-NMMA (0, 1 mM), a cell membrane-permeable competitive NOS inhibitor. (k) After incubation for 24 h, respectively, with LD100 (0, 100 μ M) and L-NMMA (1 mM L-NMMA), flow cytometric analysis of HeLa cell apoptosis is carried out with Annexin V and propidium iodide staining. To obtain more significant results, 100 μ M LD100 was used here. The relative intensity of protein bands (means ± SD) in the western blotting is quantified by using the ImageJ software and normalized through the negative control, respectively (b–g). Representative results from three independent experiments are shown (b–g and k). Data are mean of triplicate samples ± SD (ns: not statistically significant; ∗ P < 0.05; unpaired Student's t -test), and all error bars are SD.

    Techniques Used: Inhibition, Quantitative RT-PCR, Western Blot, Incubation, Fluorescence, Flow Cytometry, Agarose Gel Electrophoresis, Staining, Software, Negative Control

    cleaved caspase 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 9
    Changes of protein and mRNA expression levels of caspase-3, <t>caspase-9,</t> and PARP in the liver at 21 and 42 days of experiment. (a) The western blot assay of cleaved <t>caspase-3,</t> <t>cleaved</t> <t>caspase-9,</t> and cleaved PARP. (b) The relative protein expression levels of cleaved caspase-3, cleaved caspase-9, and cleaved PARP. (c) The melting curve analysis of caspase-3, caspase-9, and PARP. (d) The relative mRNA expression levels of caspase-3, caspase-9, and PARP. Data are presented as the means ± standard deviation ( n = 8). ∗ p < 0.05 and ∗∗ p < 0.01, compared with the control group.
    Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver"

    Article Title: Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2020/1359164

    Changes of protein and mRNA expression levels of caspase-3, caspase-9, and PARP in the liver at 21 and 42 days of experiment. (a) The western blot assay of cleaved caspase-3, cleaved caspase-9, and cleaved PARP. (b) The relative protein expression levels of cleaved caspase-3, cleaved caspase-9, and cleaved PARP. (c) The melting curve analysis of caspase-3, caspase-9, and PARP. (d) The relative mRNA expression levels of caspase-3, caspase-9, and PARP. Data are presented as the means ± standard deviation ( n = 8). ∗ p < 0.05 and ∗∗ p < 0.01, compared with the control group.
    Figure Legend Snippet: Changes of protein and mRNA expression levels of caspase-3, caspase-9, and PARP in the liver at 21 and 42 days of experiment. (a) The western blot assay of cleaved caspase-3, cleaved caspase-9, and cleaved PARP. (b) The relative protein expression levels of cleaved caspase-3, cleaved caspase-9, and cleaved PARP. (c) The melting curve analysis of caspase-3, caspase-9, and PARP. (d) The relative mRNA expression levels of caspase-3, caspase-9, and PARP. Data are presented as the means ± standard deviation ( n = 8). ∗ p < 0.05 and ∗∗ p < 0.01, compared with the control group.

    Techniques Used: Expressing, Western Blot, Standard Deviation

    caspase 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 9
    Western blot analysis of cell lysates from pLXSN-flagHPV20E6- and pLXSN-NIKS cells after transfection with siRNAp53 (10 nM) or siRNA-NK (negative control siRNA, 10 nM) and incubation for 16 h with or without MG132 (10 µm). MG132 treatment induced activation of apaf-1, caspase-8, <t>caspase-9</t> and caspase-3, as well as the autophagy marker LC3BII. Histograms represent HPV20E6 and wtp53 protein levels normalized against β-actin.
    Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    caspase 9 - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Differential Regulation of Cutaneous Oncoprotein HPVE6 by wtp53, Mutant p53R248W and ΔNp63α is HPV Type Dependent"

    Article Title: Differential Regulation of Cutaneous Oncoprotein HPVE6 by wtp53, Mutant p53R248W and ΔNp63α is HPV Type Dependent

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0035540

    Western blot analysis of cell lysates from pLXSN-flagHPV20E6- and pLXSN-NIKS cells after transfection with siRNAp53 (10 nM) or siRNA-NK (negative control siRNA, 10 nM) and incubation for 16 h with or without MG132 (10 µm). MG132 treatment induced activation of apaf-1, caspase-8, caspase-9 and caspase-3, as well as the autophagy marker LC3BII. Histograms represent HPV20E6 and wtp53 protein levels normalized against β-actin.
    Figure Legend Snippet: Western blot analysis of cell lysates from pLXSN-flagHPV20E6- and pLXSN-NIKS cells after transfection with siRNAp53 (10 nM) or siRNA-NK (negative control siRNA, 10 nM) and incubation for 16 h with or without MG132 (10 µm). MG132 treatment induced activation of apaf-1, caspase-8, caspase-9 and caspase-3, as well as the autophagy marker LC3BII. Histograms represent HPV20E6 and wtp53 protein levels normalized against β-actin.

    Techniques Used: Western Blot, Transfection, Negative Control, Incubation, Activation Assay, Marker

    cleaved caspase 9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 9
    TGFβ-induced <t>caspase-9</t> cleavage requires both Par6 and TβRI activation in NMuMG 3D structures. 12-day old 3D structures of the indicated NMuMG-derived cell lines were treated with control media (DMSO alone), TGFβ1 (TGFβ), the TβRI/Smad2 inhibitor SB-431542 (SB) or TGFβ + SB for 48 hours, fixed and immunostained for zonula occludens-1 (ZO-1, green) <t>and</t> <t>cleaved</t> <t>caspase-9</t> <t>(CC9,</t> red). Dapi was used to visualize the nuclei. A . Confocal images show representative structures for each given cell line and treatment. Scale bar = 20 μm. Arrow = apoptotic nuclei. B . Average percentage of apoptotic structures was calculated per each cell type and treatment, with n = 3 for biological replicates. Two-way ANOVA for all cell lines and treatments was significant ( p < 0.001). Bonferroni post test compared differences between control and treatment for each cell line, ** p < 0.001.
    Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells"

    Article Title: Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells

    Journal: Cancer Cell International

    doi: 10.1186/1475-2867-14-19

    TGFβ-induced caspase-9 cleavage requires both Par6 and TβRI activation in NMuMG 3D structures. 12-day old 3D structures of the indicated NMuMG-derived cell lines were treated with control media (DMSO alone), TGFβ1 (TGFβ), the TβRI/Smad2 inhibitor SB-431542 (SB) or TGFβ + SB for 48 hours, fixed and immunostained for zonula occludens-1 (ZO-1, green) and cleaved caspase-9 (CC9, red). Dapi was used to visualize the nuclei. A . Confocal images show representative structures for each given cell line and treatment. Scale bar = 20 μm. Arrow = apoptotic nuclei. B . Average percentage of apoptotic structures was calculated per each cell type and treatment, with n = 3 for biological replicates. Two-way ANOVA for all cell lines and treatments was significant ( p < 0.001). Bonferroni post test compared differences between control and treatment for each cell line, ** p < 0.001.
    Figure Legend Snippet: TGFβ-induced caspase-9 cleavage requires both Par6 and TβRI activation in NMuMG 3D structures. 12-day old 3D structures of the indicated NMuMG-derived cell lines were treated with control media (DMSO alone), TGFβ1 (TGFβ), the TβRI/Smad2 inhibitor SB-431542 (SB) or TGFβ + SB for 48 hours, fixed and immunostained for zonula occludens-1 (ZO-1, green) and cleaved caspase-9 (CC9, red). Dapi was used to visualize the nuclei. A . Confocal images show representative structures for each given cell line and treatment. Scale bar = 20 μm. Arrow = apoptotic nuclei. B . Average percentage of apoptotic structures was calculated per each cell type and treatment, with n = 3 for biological replicates. Two-way ANOVA for all cell lines and treatments was significant ( p < 0.001). Bonferroni post test compared differences between control and treatment for each cell line, ** p < 0.001.

    Techniques Used: Activation Assay, Derivative Assay

    caspase 9 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 9 antibody
    DNAJB6(S) inhibits p18 Bax cleavage and <t>caspase-9</t> activation in MPP + -treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µ M MPP + for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β -Actin was used as a loading control and COX-II was used for mitochondrial marker.
    Caspase 9 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss"

    Article Title: The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2017/7982389

    DNAJB6(S) inhibits p18 Bax cleavage and caspase-9 activation in MPP + -treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µ M MPP + for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β -Actin was used as a loading control and COX-II was used for mitochondrial marker.
    Figure Legend Snippet: DNAJB6(S) inhibits p18 Bax cleavage and caspase-9 activation in MPP + -treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µ M MPP + for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β -Actin was used as a loading control and COX-II was used for mitochondrial marker.

    Techniques Used: Activation Assay, Stable Transfection, Transfection, Expressing, Western Blot, Marker

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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc cleaved caspase 9
    Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti cleaved caspase9
    Mfn2 siRNA inhibited endoplasmic reticulum- (ER-) mitochondria-dependent apoptosis. (a) Cell apoptosis was evaluated by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. (b) HL-1 cells under TM stimulated for 24 hours. Mfn siRNA inhibited the Bax/Bcl-2 ratio and inhibited caspase3 and <t>caspase9</t> activation (c). Data are mean ± SEM, n = 4 independent experiments, ∗ p < 0.05, # p < 0.01, ANOVA with Bonferroni posttest.
    Anti Cleaved Caspase9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase9/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti cleaved caspase 9
    Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 <t>and</t> <t>cleaved</t> <t>caspase-9.</t> *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis
    Anti Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit polyclonal anti cleaved caspase 9 asp353
    The specific SOD1 inhibition activates mitochondria-dependent apoptosis of cancer cells. (a, b) RT-qPCR (a) and western blotting (b) examination of p53 mRNA levels and protein content in 50 (RT-qPCR) or 0-100 μ M (western blotting) LD100-treated HeLa, DU145, and RWPE-1 cells. (c) The protein levels of p21 and p19 in HeLa and DU145 cells are evaluated by western blotting analysis, after the cells are incubated with varied concentrations of LD100 for 24 h. β -Actin is used as an internal control (b, c). (d) The protein levels of proto- <t>and</t> <t>cleaved</t> <t>caspase-9</t> and -3 in HeLa cells are evaluated by western blotting analysis, after HeLa cells are incubated with varied concentrations of LD100 for 24 h. (e) The time dependence of the protein levels of proto- and cleaved caspase-9 is evaluated by western blotting analysis in 50 μ M LD100-treated HeLa cells. (f, h) HeLa cells are incubated with varied concentrations of LD100 for 24 h prior to western blotting evaluation of Bcl-2 (f) and cleaved PARP1 (h) protein levels. (g) Rh123 fluorescence that is positively correlated with mitochondrial membrane potential is measured by flow cytometry in HeLa, DU145, and RWPE-1 cells after incubated with varied concentrations of LD100 for 24 h. (i) DNA fragmentation in HeLa cells is assayed using agarose gel electrophoresis after incubated with varied concentrations of LD100 for 24 h. (j) The peroxynitrite content in HeLa cells is presented by DHR123 fluorescence measured with flow cytometry after incubation for 24 h, respectively, with LD100 (0, 50, and 100 μ M) and L-NMMA (0, 1 mM), a cell membrane-permeable competitive NOS inhibitor. (k) After incubation for 24 h, respectively, with LD100 (0, 100 μ M) and L-NMMA (1 mM L-NMMA), flow cytometric analysis of HeLa cell apoptosis is carried out with Annexin V and propidium iodide staining. To obtain more significant results, 100 μ M LD100 was used here. The relative intensity of protein bands (means ± SD) in the western blotting is quantified by using the ImageJ software and normalized through the negative control, respectively (b–g). Representative results from three independent experiments are shown (b–g and k). Data are mean of triplicate samples ± SD (ns: not statistically significant; ∗ P < 0.05; unpaired Student's t -test), and all error bars are SD.
    Rabbit Polyclonal Anti Cleaved Caspase 9 Asp353, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc caspase 9
    Western blot analysis of cell lysates from pLXSN-flagHPV20E6- and pLXSN-NIKS cells after transfection with siRNAp53 (10 nM) or siRNA-NK (negative control siRNA, 10 nM) and incubation for 16 h with or without MG132 (10 µm). MG132 treatment induced activation of apaf-1, caspase-8, <t>caspase-9</t> and caspase-3, as well as the autophagy marker LC3BII. Histograms represent HPV20E6 and wtp53 protein levels normalized against β-actin.
    Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc caspase 9 antibody
    DNAJB6(S) inhibits p18 Bax cleavage and <t>caspase-9</t> activation in MPP + -treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µ M MPP + for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β -Actin was used as a loading control and COX-II was used for mitochondrial marker.
    Caspase 9 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mfn2 siRNA inhibited endoplasmic reticulum- (ER-) mitochondria-dependent apoptosis. (a) Cell apoptosis was evaluated by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. (b) HL-1 cells under TM stimulated for 24 hours. Mfn siRNA inhibited the Bax/Bcl-2 ratio and inhibited caspase3 and caspase9 activation (c). Data are mean ± SEM, n = 4 independent experiments, ∗ p < 0.05, # p < 0.01, ANOVA with Bonferroni posttest.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Hyperglycemia Induces Endoplasmic Reticulum Stress in Atrial Cardiomyocytes, and Mitofusin-2 Downregulation Prevents Mitochondrial Dysfunction and Subsequent Cell Death

    doi: 10.1155/2020/6569728

    Figure Lengend Snippet: Mfn2 siRNA inhibited endoplasmic reticulum- (ER-) mitochondria-dependent apoptosis. (a) Cell apoptosis was evaluated by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. (b) HL-1 cells under TM stimulated for 24 hours. Mfn siRNA inhibited the Bax/Bcl-2 ratio and inhibited caspase3 and caspase9 activation (c). Data are mean ± SEM, n = 4 independent experiments, ∗ p < 0.05, # p < 0.01, ANOVA with Bonferroni posttest.

    Article Snippet: Proteins (60 μ g) were separated by SDS-PAGE and transferred to PVDF membranes, blocked with TBST containing 5% nonfat milk and incubated overnight at 4°C with the following antibodies: anti-CHOP (#2895), anti-caspase9 (#9504S), anti-caspase3 (#9662S), anti-cleaved-caspase9 (#9509S), anti-cleaved-caspase3 (Asp175) (#9661S), and anti-MCU (D2Z3B) (#14997S) from Cell Signaling Technology; anti-GRP78 (ab21685), anti-Mfn2 (ab56889), anti-VDAC1 (ab15895), anti-Bax (ab32503), anti-Bcl-2 (ab59348), and anti-Mn-SOD (ab13533) from Abcam; and anti- β -actin antibody as a loading control from Proteintech.

    Techniques: Flow Cytometry, Staining, Activation Assay

    Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 and cleaved caspase-9. *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Maresin-1 Inhibits Oxidative Stress and Inflammation and Promotes Apoptosis in a Mouse Model of Caerulein-Induced Acute Pancreatitis

    doi: 10.12659/MSM.917380

    Figure Lengend Snippet: Mice with caerulein-induced acute pancreatitis (AP) treated with maresin-1 (MaR1) showed increased expression of apoptosis-associated proteins. Western blot shows the expression levels of Bcl-2, Bax, cleaved caspase-3 and cleaved caspase-9. *** P<0.001 vs. control; ## P<0.01, ### P<0.001 vs. AP. MaR1 – maresin-1; AP – acute pancreatitis

    Article Snippet: The primary antibodies were obtained from Cell Signaling Technology (Boston, MA, USA) and included anti-Bcl-2 (3498S), anti-Bax (14796S), anti-cleaved caspase-3 (9664S), anti-cleaved caspase-9 (9509T), anti-TLR4 (14358S), anti-MyD88 (4283S), anti-phospho-NF-κB p65 (3033S), anti-NF-κB p65 (8242S), and anti-GAPDH (5174S).

    Techniques: Expressing, Western Blot

    The specific SOD1 inhibition activates mitochondria-dependent apoptosis of cancer cells. (a, b) RT-qPCR (a) and western blotting (b) examination of p53 mRNA levels and protein content in 50 (RT-qPCR) or 0-100 μ M (western blotting) LD100-treated HeLa, DU145, and RWPE-1 cells. (c) The protein levels of p21 and p19 in HeLa and DU145 cells are evaluated by western blotting analysis, after the cells are incubated with varied concentrations of LD100 for 24 h. β -Actin is used as an internal control (b, c). (d) The protein levels of proto- and cleaved caspase-9 and -3 in HeLa cells are evaluated by western blotting analysis, after HeLa cells are incubated with varied concentrations of LD100 for 24 h. (e) The time dependence of the protein levels of proto- and cleaved caspase-9 is evaluated by western blotting analysis in 50 μ M LD100-treated HeLa cells. (f, h) HeLa cells are incubated with varied concentrations of LD100 for 24 h prior to western blotting evaluation of Bcl-2 (f) and cleaved PARP1 (h) protein levels. (g) Rh123 fluorescence that is positively correlated with mitochondrial membrane potential is measured by flow cytometry in HeLa, DU145, and RWPE-1 cells after incubated with varied concentrations of LD100 for 24 h. (i) DNA fragmentation in HeLa cells is assayed using agarose gel electrophoresis after incubated with varied concentrations of LD100 for 24 h. (j) The peroxynitrite content in HeLa cells is presented by DHR123 fluorescence measured with flow cytometry after incubation for 24 h, respectively, with LD100 (0, 50, and 100 μ M) and L-NMMA (0, 1 mM), a cell membrane-permeable competitive NOS inhibitor. (k) After incubation for 24 h, respectively, with LD100 (0, 100 μ M) and L-NMMA (1 mM L-NMMA), flow cytometric analysis of HeLa cell apoptosis is carried out with Annexin V and propidium iodide staining. To obtain more significant results, 100 μ M LD100 was used here. The relative intensity of protein bands (means ± SD) in the western blotting is quantified by using the ImageJ software and normalized through the negative control, respectively (b–g). Representative results from three independent experiments are shown (b–g and k). Data are mean of triplicate samples ± SD (ns: not statistically significant; ∗ P < 0.05; unpaired Student's t -test), and all error bars are SD.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network

    doi: 10.1155/2019/9706792

    Figure Lengend Snippet: The specific SOD1 inhibition activates mitochondria-dependent apoptosis of cancer cells. (a, b) RT-qPCR (a) and western blotting (b) examination of p53 mRNA levels and protein content in 50 (RT-qPCR) or 0-100 μ M (western blotting) LD100-treated HeLa, DU145, and RWPE-1 cells. (c) The protein levels of p21 and p19 in HeLa and DU145 cells are evaluated by western blotting analysis, after the cells are incubated with varied concentrations of LD100 for 24 h. β -Actin is used as an internal control (b, c). (d) The protein levels of proto- and cleaved caspase-9 and -3 in HeLa cells are evaluated by western blotting analysis, after HeLa cells are incubated with varied concentrations of LD100 for 24 h. (e) The time dependence of the protein levels of proto- and cleaved caspase-9 is evaluated by western blotting analysis in 50 μ M LD100-treated HeLa cells. (f, h) HeLa cells are incubated with varied concentrations of LD100 for 24 h prior to western blotting evaluation of Bcl-2 (f) and cleaved PARP1 (h) protein levels. (g) Rh123 fluorescence that is positively correlated with mitochondrial membrane potential is measured by flow cytometry in HeLa, DU145, and RWPE-1 cells after incubated with varied concentrations of LD100 for 24 h. (i) DNA fragmentation in HeLa cells is assayed using agarose gel electrophoresis after incubated with varied concentrations of LD100 for 24 h. (j) The peroxynitrite content in HeLa cells is presented by DHR123 fluorescence measured with flow cytometry after incubation for 24 h, respectively, with LD100 (0, 50, and 100 μ M) and L-NMMA (0, 1 mM), a cell membrane-permeable competitive NOS inhibitor. (k) After incubation for 24 h, respectively, with LD100 (0, 100 μ M) and L-NMMA (1 mM L-NMMA), flow cytometric analysis of HeLa cell apoptosis is carried out with Annexin V and propidium iodide staining. To obtain more significant results, 100 μ M LD100 was used here. The relative intensity of protein bands (means ± SD) in the western blotting is quantified by using the ImageJ software and normalized through the negative control, respectively (b–g). Representative results from three independent experiments are shown (b–g and k). Data are mean of triplicate samples ± SD (ns: not statistically significant; ∗ P < 0.05; unpaired Student's t -test), and all error bars are SD.

    Article Snippet: Rabbit polyclonal anti-caspase-9 (Cat# 9502; RRID:AB_2068621) and rabbit polyclonal anti-cleaved caspase-9 (Asp353) (Cat# 9509; RRID:AB_2073476) were purchased from Cell Signaling Technology.

    Techniques: Inhibition, Quantitative RT-PCR, Western Blot, Incubation, Fluorescence, Flow Cytometry, Agarose Gel Electrophoresis, Staining, Software, Negative Control

    Western blot analysis of cell lysates from pLXSN-flagHPV20E6- and pLXSN-NIKS cells after transfection with siRNAp53 (10 nM) or siRNA-NK (negative control siRNA, 10 nM) and incubation for 16 h with or without MG132 (10 µm). MG132 treatment induced activation of apaf-1, caspase-8, caspase-9 and caspase-3, as well as the autophagy marker LC3BII. Histograms represent HPV20E6 and wtp53 protein levels normalized against β-actin.

    Journal: PLoS ONE

    Article Title: Differential Regulation of Cutaneous Oncoprotein HPVE6 by wtp53, Mutant p53R248W and ΔNp63α is HPV Type Dependent

    doi: 10.1371/journal.pone.0035540

    Figure Lengend Snippet: Western blot analysis of cell lysates from pLXSN-flagHPV20E6- and pLXSN-NIKS cells after transfection with siRNAp53 (10 nM) or siRNA-NK (negative control siRNA, 10 nM) and incubation for 16 h with or without MG132 (10 µm). MG132 treatment induced activation of apaf-1, caspase-8, caspase-9 and caspase-3, as well as the autophagy marker LC3BII. Histograms represent HPV20E6 and wtp53 protein levels normalized against β-actin.

    Article Snippet: Primary antibodies against Apaf-1 (cat#4452), caspase-3 (cat#9662), caspase-9 (cat#9509), caspase-8 (cat#9746), LC3B (cat#4108), Bak (cat#3792), Bax (cat#2774), phospho-c-Jun (ser63) (cat#9261) and c-Jun (cat#9165) were purchased from Cell Signaling (Frankfurt am Main, Germany).

    Techniques: Western Blot, Transfection, Negative Control, Incubation, Activation Assay, Marker

    DNAJB6(S) inhibits p18 Bax cleavage and caspase-9 activation in MPP + -treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µ M MPP + for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β -Actin was used as a loading control and COX-II was used for mitochondrial marker.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss

    doi: 10.1155/2017/7982389

    Figure Lengend Snippet: DNAJB6(S) inhibits p18 Bax cleavage and caspase-9 activation in MPP + -treated cells. LN18 cells stably transfected by pMOCK or pDNAJB6(S) were treated with or without 500 µ M MPP + for 48 h. The cells were harvested, and mitochondrial and cytosolic fractions (F) were prepared. The expression levels of p18 Bax and caspase-9 cleavage were analyzed by western blotting. An anti-Bax antibody detected both full-length Bax (20 kDa) and p18 Bax. β -Actin was used as a loading control and COX-II was used for mitochondrial marker.

    Article Snippet: FBS and Mitochondrial Isolation kit are from Thermo Scientific (Waltham, MA), glutamine and penicillin/streptomycin are from Welgene (Kyungsan, Korea), MPP + and β -actin antibody are bought from Sigma-Aldrich (St. Louis, MO, USA), restriction enzymes Bam HI and Hin dIII (NEB) and RNeasy Mini Spin Columns are from Qiagen (Hiden, Germany), Transcriptor First-Strand cDNA Synthesis kit is from Roche Diagnostics (Basel, Switzerland), anti-DNAJB6 antibody (1 : 1000) is from Abnova (Taipei, Taiwan, H00010049-M01), anti-c-myc antibody (1 : 3000) is from Invitrogen (R950-25), anti-Bax antibody (1 : 1,000) is from Cell Signaling (2772), anti-COX-2 antibody (1 : 2000) is from Cell Signaling (4842), and anticleaved caspase-9 antibody (1 : 1,000) is from Cell Signaling (9509).

    Techniques: Activation Assay, Stable Transfection, Transfection, Expressing, Western Blot, Marker