caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and <t>caspase</t> <t>7</t> was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. ∗ p < 0.05.
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Kaempferol Protects Cell Damage in In Vitro Ischemia Reperfusion Model in Rat Neuronal PC12 Cells"

    Article Title: Kaempferol Protects Cell Damage in In Vitro Ischemia Reperfusion Model in Rat Neuronal PC12 Cells

    Journal: BioMed Research International

    doi: 10.1155/2020/2461079

    KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and caspase 7 was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. ∗ p < 0.05.
    Figure Legend Snippet: KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and caspase 7 was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. ∗ p < 0.05.

    Techniques Used: TUNEL Assay, Expressing, Western Blot, Real-time Polymerase Chain Reaction

    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and <t>caspase</t> <t>7</t> was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. ∗ p < 0.05.
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Kaempferol Protects Cell Damage in In Vitro Ischemia Reperfusion Model in Rat Neuronal PC12 Cells"

    Article Title: Kaempferol Protects Cell Damage in In Vitro Ischemia Reperfusion Model in Rat Neuronal PC12 Cells

    Journal: BioMed Research International

    doi: 10.1155/2020/2461079

    KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and caspase 7 was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. ∗ p < 0.05.
    Figure Legend Snippet: KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and caspase 7 was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. ∗ p < 0.05.

    Techniques Used: TUNEL Assay, Expressing, Western Blot, Real-time Polymerase Chain Reaction

    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti cleaved caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti cleaved caspase 7
    GSK-3 β inhibition prevents the CLP-induced hepatic apoptosis. (a) Mice were subjected to CLP and treated with either SB216763 (25 mg/kg, i.p.) or vehicle (DMSO). Liver samples were harvested at 20 h after CLP. The caspase-3 activity was measured. Data are shown as mean ± SD. n = 6 per group. # P < 0.01 compared with vehicle-treated group. (b) The expression levels of the cleaved caspase-3 and <t>the</t> <t>cleaved</t> <t>caspase-7</t> were examined by western blotting analysis.
    Rabbit Anti Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "GSK-3 β Inhibition Attenuates CLP-Induced Liver Injury by Reducing Inflammation and Hepatic Cell Apoptosis"

    Article Title: GSK-3 β Inhibition Attenuates CLP-Induced Liver Injury by Reducing Inflammation and Hepatic Cell Apoptosis

    Journal: Mediators of Inflammation

    doi: 10.1155/2014/629507

    GSK-3 β inhibition prevents the CLP-induced hepatic apoptosis. (a) Mice were subjected to CLP and treated with either SB216763 (25 mg/kg, i.p.) or vehicle (DMSO). Liver samples were harvested at 20 h after CLP. The caspase-3 activity was measured. Data are shown as mean ± SD. n = 6 per group. # P < 0.01 compared with vehicle-treated group. (b) The expression levels of the cleaved caspase-3 and the cleaved caspase-7 were examined by western blotting analysis.
    Figure Legend Snippet: GSK-3 β inhibition prevents the CLP-induced hepatic apoptosis. (a) Mice were subjected to CLP and treated with either SB216763 (25 mg/kg, i.p.) or vehicle (DMSO). Liver samples were harvested at 20 h after CLP. The caspase-3 activity was measured. Data are shown as mean ± SD. n = 6 per group. # P < 0.01 compared with vehicle-treated group. (b) The expression levels of the cleaved caspase-3 and the cleaved caspase-7 were examined by western blotting analysis.

    Techniques Used: Inhibition, Activity Assay, Expressing, Western Blot

    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    Differential protein expression in control versus 3xTgAD lipid raft extracts. (a) Proportionately drawn Venn diagram analysis of reliably identified proteins from control lipid rafts (blue line) and 3xTgAD rafts (red line). (b) Total protein loading control for centrifugal fraction 2. A total of 50 mg of fraction 2 protein was loaded and stained with SYPRO Ruby and scanned using a phosphorimager. (c)–(n). Representative western blots from multiple expression analysis experiments for differential presentation of proteins in fraction 2 extracts from control (blue) or 3xTgAD mice (red). Associated with each panel (c)–(n) the associated histograms represent the mean ± s.e. mean of protein expression intensity (measured in ((AU-B)/px 2 )) from at least three separate experiments. * P < .05; ** P < .01; *** P < .001. Protein abbreviations are as follows. FAK: focal adhesion kinase; Pyk2: proline-rich tyrosine kinase 2; GIT-1: GRK interactor-1; Jak2: Janus kinase 2; Crk: v-Crk avian sarcoma virus CT10 oncogene homolog; Fyn: Fyn tyrosine kinase; IRS-1: insulin receptor substrate-1; casp 7: caspase 7; mTOR: mammalian target of rapamycin; Grin2: G protein-regulated inducer of neurite outgrowth 2; IGF-1R: insulin-like growth factor-1 receptor.
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Complex and Multidimensional Lipid Raft Alterations in a Murine Model of Alzheimer's Disease"

    Article Title: Complex and Multidimensional Lipid Raft Alterations in a Murine Model of Alzheimer's Disease

    Journal: International Journal of Alzheimer's Disease

    doi: 10.4061/2010/604792

    Differential protein expression in control versus 3xTgAD lipid raft extracts. (a) Proportionately drawn Venn diagram analysis of reliably identified proteins from control lipid rafts (blue line) and 3xTgAD rafts (red line). (b) Total protein loading control for centrifugal fraction 2. A total of 50 mg of fraction 2 protein was loaded and stained with SYPRO Ruby and scanned using a phosphorimager. (c)–(n). Representative western blots from multiple expression analysis experiments for differential presentation of proteins in fraction 2 extracts from control (blue) or 3xTgAD mice (red). Associated with each panel (c)–(n) the associated histograms represent the mean ± s.e. mean of protein expression intensity (measured in ((AU-B)/px 2 )) from at least three separate experiments. * P < .05; ** P < .01; *** P < .001. Protein abbreviations are as follows. FAK: focal adhesion kinase; Pyk2: proline-rich tyrosine kinase 2; GIT-1: GRK interactor-1; Jak2: Janus kinase 2; Crk: v-Crk avian sarcoma virus CT10 oncogene homolog; Fyn: Fyn tyrosine kinase; IRS-1: insulin receptor substrate-1; casp 7: caspase 7; mTOR: mammalian target of rapamycin; Grin2: G protein-regulated inducer of neurite outgrowth 2; IGF-1R: insulin-like growth factor-1 receptor.
    Figure Legend Snippet: Differential protein expression in control versus 3xTgAD lipid raft extracts. (a) Proportionately drawn Venn diagram analysis of reliably identified proteins from control lipid rafts (blue line) and 3xTgAD rafts (red line). (b) Total protein loading control for centrifugal fraction 2. A total of 50 mg of fraction 2 protein was loaded and stained with SYPRO Ruby and scanned using a phosphorimager. (c)–(n). Representative western blots from multiple expression analysis experiments for differential presentation of proteins in fraction 2 extracts from control (blue) or 3xTgAD mice (red). Associated with each panel (c)–(n) the associated histograms represent the mean ± s.e. mean of protein expression intensity (measured in ((AU-B)/px 2 )) from at least three separate experiments. * P < .05; ** P < .01; *** P < .001. Protein abbreviations are as follows. FAK: focal adhesion kinase; Pyk2: proline-rich tyrosine kinase 2; GIT-1: GRK interactor-1; Jak2: Janus kinase 2; Crk: v-Crk avian sarcoma virus CT10 oncogene homolog; Fyn: Fyn tyrosine kinase; IRS-1: insulin receptor substrate-1; casp 7: caspase 7; mTOR: mammalian target of rapamycin; Grin2: G protein-regulated inducer of neurite outgrowth 2; IGF-1R: insulin-like growth factor-1 receptor.

    Techniques Used: Expressing, Staining, Western Blot

    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc caspase 7
    Effect of buthionine sulfoximine (BSO) and 17β-estradiol (E 2 ) on Bcl-2 family protein expression and mitochondrial function in MCF-7 and MCF-7:2A cells. (a) Western blot analysis for pBcl-2, Bcl-2, Bcl-x L , and Bax protein expression in parental MCF-7 cells and MCF-7:2A cells following 48 h of treatment with ethanol vehicle (Control), 1 nM E 2 , 100 μM BSO, or E 2 + BSO. Equal loading was confirmed by reprobing with an antibody against β-actin. (b) Small interfering RNA (siRNA) knockdown of Bcl-2 partially sensitizes MCF-7:2A cells to E 2 -induced apoptosis. Cells were transfected with 100 nM siRNA-Bcl-2 or siRNA-Con (control) and expression levels of Bcl-2 was determined by immunoblot analysis (top). Annexin V staining (bottom) showing the effects of siRNA-con and siRNA-Bcl-2 on apoptosis induced by estradiol treatment in MCF-7:2A cells. *, p < 0.001. (c) Loss of mitochondrial potential in MCF-7:2A cells was determined by rhodamine 123 (Rh123) retention assay. The percentage of cells retaining Rh123 in each treatment group was compared with untreated control. (d) Cytochrome c release from the mitochondria to the cytosol after treatment with E 2 alone or BSO and E 2 for 48 h was determined as described in Materials and methods. Anti-Cox IV antibody was used as a control to demonstrate that mitochondrial protein fractionation was successfully achieved. (e) Cleavage of <t>caspase</t> <t>7</t> and poly(ADP-ribose) polymerase (PARP) (72 h) was assessed by western blot using specific antibodies. The upper band of caspase 7 represents the full-length protein and the lower band (p20, arrow) represents the cleaved activated product; NS, nonspecific. Full length PARP is approximately 116 kDa; cleaved (active) PARP is 85 kDa (arrow). The results are representative of three independent experiments.
    Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Buthionine sulfoximine sensitizes antihormone-resistant human breast cancer cells to estrogen-induced apoptosis"

    Article Title: Buthionine sulfoximine sensitizes antihormone-resistant human breast cancer cells to estrogen-induced apoptosis

    Journal: Breast Cancer Research : BCR

    doi: 10.1186/bcr2208

    Effect of buthionine sulfoximine (BSO) and 17β-estradiol (E 2 ) on Bcl-2 family protein expression and mitochondrial function in MCF-7 and MCF-7:2A cells. (a) Western blot analysis for pBcl-2, Bcl-2, Bcl-x L , and Bax protein expression in parental MCF-7 cells and MCF-7:2A cells following 48 h of treatment with ethanol vehicle (Control), 1 nM E 2 , 100 μM BSO, or E 2 + BSO. Equal loading was confirmed by reprobing with an antibody against β-actin. (b) Small interfering RNA (siRNA) knockdown of Bcl-2 partially sensitizes MCF-7:2A cells to E 2 -induced apoptosis. Cells were transfected with 100 nM siRNA-Bcl-2 or siRNA-Con (control) and expression levels of Bcl-2 was determined by immunoblot analysis (top). Annexin V staining (bottom) showing the effects of siRNA-con and siRNA-Bcl-2 on apoptosis induced by estradiol treatment in MCF-7:2A cells. *, p < 0.001. (c) Loss of mitochondrial potential in MCF-7:2A cells was determined by rhodamine 123 (Rh123) retention assay. The percentage of cells retaining Rh123 in each treatment group was compared with untreated control. (d) Cytochrome c release from the mitochondria to the cytosol after treatment with E 2 alone or BSO and E 2 for 48 h was determined as described in Materials and methods. Anti-Cox IV antibody was used as a control to demonstrate that mitochondrial protein fractionation was successfully achieved. (e) Cleavage of caspase 7 and poly(ADP-ribose) polymerase (PARP) (72 h) was assessed by western blot using specific antibodies. The upper band of caspase 7 represents the full-length protein and the lower band (p20, arrow) represents the cleaved activated product; NS, nonspecific. Full length PARP is approximately 116 kDa; cleaved (active) PARP is 85 kDa (arrow). The results are representative of three independent experiments.
    Figure Legend Snippet: Effect of buthionine sulfoximine (BSO) and 17β-estradiol (E 2 ) on Bcl-2 family protein expression and mitochondrial function in MCF-7 and MCF-7:2A cells. (a) Western blot analysis for pBcl-2, Bcl-2, Bcl-x L , and Bax protein expression in parental MCF-7 cells and MCF-7:2A cells following 48 h of treatment with ethanol vehicle (Control), 1 nM E 2 , 100 μM BSO, or E 2 + BSO. Equal loading was confirmed by reprobing with an antibody against β-actin. (b) Small interfering RNA (siRNA) knockdown of Bcl-2 partially sensitizes MCF-7:2A cells to E 2 -induced apoptosis. Cells were transfected with 100 nM siRNA-Bcl-2 or siRNA-Con (control) and expression levels of Bcl-2 was determined by immunoblot analysis (top). Annexin V staining (bottom) showing the effects of siRNA-con and siRNA-Bcl-2 on apoptosis induced by estradiol treatment in MCF-7:2A cells. *, p < 0.001. (c) Loss of mitochondrial potential in MCF-7:2A cells was determined by rhodamine 123 (Rh123) retention assay. The percentage of cells retaining Rh123 in each treatment group was compared with untreated control. (d) Cytochrome c release from the mitochondria to the cytosol after treatment with E 2 alone or BSO and E 2 for 48 h was determined as described in Materials and methods. Anti-Cox IV antibody was used as a control to demonstrate that mitochondrial protein fractionation was successfully achieved. (e) Cleavage of caspase 7 and poly(ADP-ribose) polymerase (PARP) (72 h) was assessed by western blot using specific antibodies. The upper band of caspase 7 represents the full-length protein and the lower band (p20, arrow) represents the cleaved activated product; NS, nonspecific. Full length PARP is approximately 116 kDa; cleaved (active) PARP is 85 kDa (arrow). The results are representative of three independent experiments.

    Techniques Used: Expressing, Western Blot, Small Interfering RNA, Transfection, Staining, Fractionation