Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

doi: 10.1155/2012/960128

Figure Lengend Snippet: The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

Article Snippet: Smad4 polyclonal antibody, p-p38 monoclonal antibody, p38, p-MEK and MEK monoclonal antibody were purchased from Cell Signaling Technology (Beverly, MA, USA); mouse anti-human α -SMA monoclonal antibody and Trizol reagent were purchased from Sigma (St Louis, MO, USA).

Techniques: Inhibition, Western Blot, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

doi: 10.1155/2012/960128

Figure Lengend Snippet: The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

Article Snippet: Smad4 polyclonal antibody, p-p38 monoclonal antibody, p38, p-MEK and MEK monoclonal antibody were purchased from Cell Signaling Technology (Beverly, MA, USA); mouse anti-human α -SMA monoclonal antibody and Trizol reagent were purchased from Sigma (St Louis, MO, USA).

Techniques: Inhibition, Western Blot, Negative Control

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: Primers Used in qPCR

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Sequencing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Isolation, Sequencing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Isolation, Negative Control

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Expressing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

doi: 10.1371/journal.pone.0038875

Figure Lengend Snippet: A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and CCND3 protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.

Article Snippet: The antibodies used included goat anti-human ActRIIA (R&D, Minneapolis, USA), rabbit anti-human CCND1 (Abcam, Cambridge, UK), mouse anti-human CCND3 (Cell Signaling Technology, Beverly, MA, USA), mouse anti-human Actin (Cell Signaling Technology, Beverly, MA, USA), and mouse anti-human GAPDH (Ambion, Austin, Texas, USA).

Techniques: Real-time Polymerase Chain Reaction, Transfection, Western Blot