mouse anti human monoclonal antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human monoclonal antibodies
    Mouse Anti Human Monoclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human monoclonal antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human monoclonal antibodies
    Mouse Anti Human Monoclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal anti human insulin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal anti human insulin
    Mouse Monoclonal Anti Human Insulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human iκb  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human iκb
    Mouse Anti Human Iκb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human iκbα  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human iκbα
    Mouse Anti Human Iκbα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human α sma monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human α sma monoclonal antibody
    The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) <t>α</t> <t>-SMA</t> level in LX-2. The levels of <t>α</t> <t>-SMA</t> protein were determined by Western blot using anti- <t>α</t> <t>-SMA</t> antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).
    Mouse Anti Human α Sma Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human α sma monoclonal antibody/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
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    mouse anti human α sma monoclonal antibody - by Bioz Stars, 2023-02
    95/100 stars

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    1) Product Images from "Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways"

    Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2012/960128

    The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).
    Figure Legend Snippet: The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

    Techniques Used: Inhibition, Western Blot, Negative Control

    The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).
    Figure Legend Snippet: The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

    Techniques Used: Inhibition, Western Blot, Negative Control

    mouse anti human rps3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human rps3
    Primers Used in qPCR
    Mouse Anti Human Rps3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )"

    Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

    Journal: Oncology Research

    doi: 10.3727/096504018X15220594629967

    Primers Used in qPCR
    Figure Legend Snippet: Primers Used in qPCR

    Techniques Used: Sequencing

    Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)
    Figure Legend Snippet: Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)

    Techniques Used: Isolation, Sequencing

    BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.
    Figure Legend Snippet: BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.

    Techniques Used: Isolation, Negative Control

    BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.
    Figure Legend Snippet: BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.

    Techniques Used: Expressing

    GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.
    Figure Legend Snippet: GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.

    Techniques Used: Expressing, Quantitative RT-PCR

    mouse anti human ccnd3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human ccnd3
    A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and <t>CCND3</t> protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.
    Mouse Anti Human Ccnd3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression"

    Article Title: Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0038875

    A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and CCND3 protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.
    Figure Legend Snippet: A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and CCND3 protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.

    Techniques Used: Real-time Polymerase Chain Reaction, Transfection, Western Blot

    mouse anti human  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human
    Mouse Anti Human, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human monoclonal antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human monoclonal antibodies
    Mouse Anti Human Monoclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human monoclonal antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human monoclonal antibodies
    Mouse Anti Human Monoclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse anti human α sma monoclonal antibody
    The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) <t>α</t> <t>-SMA</t> level in LX-2. The levels of <t>α</t> <t>-SMA</t> protein were determined by Western blot using anti- <t>α</t> <t>-SMA</t> antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).
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    Primers Used in qPCR
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    A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and <t>CCND3</t> protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.
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    A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and <t>CCND3</t> protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.
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    Image Search Results


    The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

    doi: 10.1155/2012/960128

    Figure Lengend Snippet: The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

    Article Snippet: Smad4 polyclonal antibody, p-p38 monoclonal antibody, p38, p-MEK and MEK monoclonal antibody were purchased from Cell Signaling Technology (Beverly, MA, USA); mouse anti-human α -SMA monoclonal antibody and Trizol reagent were purchased from Sigma (St Louis, MO, USA).

    Techniques: Inhibition, Western Blot, Negative Control

    The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

    doi: 10.1155/2012/960128

    Figure Lengend Snippet: The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

    Article Snippet: Smad4 polyclonal antibody, p-p38 monoclonal antibody, p38, p-MEK and MEK monoclonal antibody were purchased from Cell Signaling Technology (Beverly, MA, USA); mouse anti-human α -SMA monoclonal antibody and Trizol reagent were purchased from Sigma (St Louis, MO, USA).

    Techniques: Inhibition, Western Blot, Negative Control

    Primers Used in qPCR

    Journal: Oncology Research

    Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

    doi: 10.3727/096504018X15220594629967

    Figure Lengend Snippet: Primers Used in qPCR

    Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

    Techniques: Sequencing

    Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)

    Journal: Oncology Research

    Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

    doi: 10.3727/096504018X15220594629967

    Figure Lengend Snippet: Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)

    Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

    Techniques: Isolation, Sequencing

    BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.

    Journal: Oncology Research

    Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

    doi: 10.3727/096504018X15220594629967

    Figure Lengend Snippet: BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.

    Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

    Techniques: Isolation, Negative Control

    BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.

    Journal: Oncology Research

    Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

    doi: 10.3727/096504018X15220594629967

    Figure Lengend Snippet: BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.

    Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

    Techniques: Expressing

    GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.

    Journal: Oncology Research

    Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

    doi: 10.3727/096504018X15220594629967

    Figure Lengend Snippet: GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.

    Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and CCND3 protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.

    Journal: PLoS ONE

    Article Title: Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

    doi: 10.1371/journal.pone.0038875

    Figure Lengend Snippet: A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and CCND3 protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.

    Article Snippet: The antibodies used included goat anti-human ActRIIA (R&D, Minneapolis, USA), rabbit anti-human CCND1 (Abcam, Cambridge, UK), mouse anti-human CCND3 (Cell Signaling Technology, Beverly, MA, USA), mouse anti-human Actin (Cell Signaling Technology, Beverly, MA, USA), and mouse anti-human GAPDH (Ambion, Austin, Texas, USA).

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Western Blot