anti cd96  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cd96
    KEGG analysis and qPCR showed that T cell receptor signaling pathway and CD112R and <t>CD96</t> are associated with AS. (a) Top 20 significantly enriched pathways of 642 DEGs. (b) CD112R and CD96 were significantly downregulated in AS patients through bioinformatics analysis. (c) qPCR validation of the expression of CD112R and CD96 between patients with AS and the healthy controls ( ∗∗ P < 0.01, ∗∗∗ P < 0.001).
    Anti Cd96, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd96/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd96 - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis"

    Article Title: CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

    Journal: BioMed Research International

    doi: 10.1155/2022/3946754

    KEGG analysis and qPCR showed that T cell receptor signaling pathway and CD112R and CD96 are associated with AS. (a) Top 20 significantly enriched pathways of 642 DEGs. (b) CD112R and CD96 were significantly downregulated in AS patients through bioinformatics analysis. (c) qPCR validation of the expression of CD112R and CD96 between patients with AS and the healthy controls ( ∗∗ P < 0.01, ∗∗∗ P < 0.001).
    Figure Legend Snippet: KEGG analysis and qPCR showed that T cell receptor signaling pathway and CD112R and CD96 are associated with AS. (a) Top 20 significantly enriched pathways of 642 DEGs. (b) CD112R and CD96 were significantly downregulated in AS patients through bioinformatics analysis. (c) qPCR validation of the expression of CD112R and CD96 between patients with AS and the healthy controls ( ∗∗ P < 0.01, ∗∗∗ P < 0.001).

    Techniques Used: Expressing

    Flow cytometry. (a) Circling gate scheme of flow cytometry analysis. Gating lymphocytes according to the size and granularity (FSC and SSC). Circle the live immune cells “CD45 live” according to CD45 and 7AAD after obtaining single cells. In the gate of “CD45 live,” NK cells (CD3 − CD56 + ) and conventional CD3 T cells (CD3 + CD56 − ) were sorted out. CD3 T cell (CD3 + CD56 − ) gate was divided into CD4 T and CD8 T cell subsets according to the expression of CD4 and CD8. (b) Histogram of the expression of CD112R and CD96 between samples and isotype. Compared to the isotype, the expression of CD112R increased slightly, but that of CD96 increased significantly. (c) Frequencies of each lymphocyte subset expressing CD96. The frequencies of CD96-positive CD45 live cells, CD3 T cells, and CD4 T cells in AS patients were lower than those in healthy controls, but no significant differences were observed on CD8 T and NK − cells. (d) Comparison of the fluorescence intensities of CD96 expressed on lymphocyte subsets. The fluorescence intensities of CD96 expressed on CD45 live cells, CD3 T cells, and CD4 + T cells were lower in AS patients than in healthy controls, but no significant differences were observed on CD8 T and NK cells ( ∗ P < 0.05, ∗∗ P < 0.01).
    Figure Legend Snippet: Flow cytometry. (a) Circling gate scheme of flow cytometry analysis. Gating lymphocytes according to the size and granularity (FSC and SSC). Circle the live immune cells “CD45 live” according to CD45 and 7AAD after obtaining single cells. In the gate of “CD45 live,” NK cells (CD3 − CD56 + ) and conventional CD3 T cells (CD3 + CD56 − ) were sorted out. CD3 T cell (CD3 + CD56 − ) gate was divided into CD4 T and CD8 T cell subsets according to the expression of CD4 and CD8. (b) Histogram of the expression of CD112R and CD96 between samples and isotype. Compared to the isotype, the expression of CD112R increased slightly, but that of CD96 increased significantly. (c) Frequencies of each lymphocyte subset expressing CD96. The frequencies of CD96-positive CD45 live cells, CD3 T cells, and CD4 T cells in AS patients were lower than those in healthy controls, but no significant differences were observed on CD8 T and NK − cells. (d) Comparison of the fluorescence intensities of CD96 expressed on lymphocyte subsets. The fluorescence intensities of CD96 expressed on CD45 live cells, CD3 T cells, and CD4 + T cells were lower in AS patients than in healthy controls, but no significant differences were observed on CD8 T and NK cells ( ∗ P < 0.05, ∗∗ P < 0.01).

    Techniques Used: Flow Cytometry, Expressing, Fluorescence

    CD96 knockdown promoted the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the shCD96 group was significantly inhibited compared to shNC group. (b) Western blotting showed that the level of p-Erk/Erk was significantly increased in the shCD96 group compared to the shNC group, but there was no significant difference observed in p-Akt/Akt. (c) Compared to the shNC group, the levels of TNF- α , IL-6, IL-17A, IFN- γ , IL-23, and IFN- γ were significantly increased in the cell culture supernatants of the shCD96 group ( ∗ P < 0.05, ∗∗ P < 0.01).
    Figure Legend Snippet: CD96 knockdown promoted the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the shCD96 group was significantly inhibited compared to shNC group. (b) Western blotting showed that the level of p-Erk/Erk was significantly increased in the shCD96 group compared to the shNC group, but there was no significant difference observed in p-Akt/Akt. (c) Compared to the shNC group, the levels of TNF- α , IL-6, IL-17A, IFN- γ , IL-23, and IFN- γ were significantly increased in the cell culture supernatants of the shCD96 group ( ∗ P < 0.05, ∗∗ P < 0.01).

    Techniques Used: Expressing, Western Blot, Cell Culture

    CD96 overexpression alleviated the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the CD96 group was significantly increased compared to the vector group. (b) Western blotting showed that the level of p-Erk/Erk was significantly downregulated in the CD96 group compared to vector group, but no significant difference was detected in p-Akt/Akt. (c) Compared to the vector group, the levels of TNF- α , IL-6, IFN- γ , IL-17A, and IL-23 were significantly decreased in the cell culture supernatants of the CD96 group ( ∗ P < 0.05).
    Figure Legend Snippet: CD96 overexpression alleviated the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the CD96 group was significantly increased compared to the vector group. (b) Western blotting showed that the level of p-Erk/Erk was significantly downregulated in the CD96 group compared to vector group, but no significant difference was detected in p-Akt/Akt. (c) Compared to the vector group, the levels of TNF- α , IL-6, IFN- γ , IL-17A, and IL-23 were significantly decreased in the cell culture supernatants of the CD96 group ( ∗ P < 0.05).

    Techniques Used: Over Expression, Expressing, Plasmid Preparation, Western Blot, Cell Culture

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    Cell Signaling Technology Inc anti cd96
    KEGG analysis and qPCR showed that T cell receptor signaling pathway and CD112R and <t>CD96</t> are associated with AS. (a) Top 20 significantly enriched pathways of 642 DEGs. (b) CD112R and CD96 were significantly downregulated in AS patients through bioinformatics analysis. (c) qPCR validation of the expression of CD112R and CD96 between patients with AS and the healthy controls ( ∗∗ P < 0.01, ∗∗∗ P < 0.001).
    Anti Cd96, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd96/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd96 - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

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    KEGG analysis and qPCR showed that T cell receptor signaling pathway and CD112R and CD96 are associated with AS. (a) Top 20 significantly enriched pathways of 642 DEGs. (b) CD112R and CD96 were significantly downregulated in AS patients through bioinformatics analysis. (c) qPCR validation of the expression of CD112R and CD96 between patients with AS and the healthy controls ( ∗∗ P < 0.01, ∗∗∗ P < 0.001).

    Journal: BioMed Research International

    Article Title: CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

    doi: 10.1155/2022/3946754

    Figure Lengend Snippet: KEGG analysis and qPCR showed that T cell receptor signaling pathway and CD112R and CD96 are associated with AS. (a) Top 20 significantly enriched pathways of 642 DEGs. (b) CD112R and CD96 were significantly downregulated in AS patients through bioinformatics analysis. (c) qPCR validation of the expression of CD112R and CD96 between patients with AS and the healthy controls ( ∗∗ P < 0.01, ∗∗∗ P < 0.001).

    Article Snippet: The protein levels were detected using anti-CD96, anti-p-Akt, anti-Akt, anti-p-ERK, anti-ERK, and anti-GAPDH Abs and horseradish peroxidase- (HRP-) conjugated anti-mouse/rabbit IgG (Cell Signaling Technology, Beverly, MA, USA).

    Techniques: Expressing

    Flow cytometry. (a) Circling gate scheme of flow cytometry analysis. Gating lymphocytes according to the size and granularity (FSC and SSC). Circle the live immune cells “CD45 live” according to CD45 and 7AAD after obtaining single cells. In the gate of “CD45 live,” NK cells (CD3 − CD56 + ) and conventional CD3 T cells (CD3 + CD56 − ) were sorted out. CD3 T cell (CD3 + CD56 − ) gate was divided into CD4 T and CD8 T cell subsets according to the expression of CD4 and CD8. (b) Histogram of the expression of CD112R and CD96 between samples and isotype. Compared to the isotype, the expression of CD112R increased slightly, but that of CD96 increased significantly. (c) Frequencies of each lymphocyte subset expressing CD96. The frequencies of CD96-positive CD45 live cells, CD3 T cells, and CD4 T cells in AS patients were lower than those in healthy controls, but no significant differences were observed on CD8 T and NK − cells. (d) Comparison of the fluorescence intensities of CD96 expressed on lymphocyte subsets. The fluorescence intensities of CD96 expressed on CD45 live cells, CD3 T cells, and CD4 + T cells were lower in AS patients than in healthy controls, but no significant differences were observed on CD8 T and NK cells ( ∗ P < 0.05, ∗∗ P < 0.01).

    Journal: BioMed Research International

    Article Title: CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

    doi: 10.1155/2022/3946754

    Figure Lengend Snippet: Flow cytometry. (a) Circling gate scheme of flow cytometry analysis. Gating lymphocytes according to the size and granularity (FSC and SSC). Circle the live immune cells “CD45 live” according to CD45 and 7AAD after obtaining single cells. In the gate of “CD45 live,” NK cells (CD3 − CD56 + ) and conventional CD3 T cells (CD3 + CD56 − ) were sorted out. CD3 T cell (CD3 + CD56 − ) gate was divided into CD4 T and CD8 T cell subsets according to the expression of CD4 and CD8. (b) Histogram of the expression of CD112R and CD96 between samples and isotype. Compared to the isotype, the expression of CD112R increased slightly, but that of CD96 increased significantly. (c) Frequencies of each lymphocyte subset expressing CD96. The frequencies of CD96-positive CD45 live cells, CD3 T cells, and CD4 T cells in AS patients were lower than those in healthy controls, but no significant differences were observed on CD8 T and NK − cells. (d) Comparison of the fluorescence intensities of CD96 expressed on lymphocyte subsets. The fluorescence intensities of CD96 expressed on CD45 live cells, CD3 T cells, and CD4 + T cells were lower in AS patients than in healthy controls, but no significant differences were observed on CD8 T and NK cells ( ∗ P < 0.05, ∗∗ P < 0.01).

    Article Snippet: The protein levels were detected using anti-CD96, anti-p-Akt, anti-Akt, anti-p-ERK, anti-ERK, and anti-GAPDH Abs and horseradish peroxidase- (HRP-) conjugated anti-mouse/rabbit IgG (Cell Signaling Technology, Beverly, MA, USA).

    Techniques: Flow Cytometry, Expressing, Fluorescence

    CD96 knockdown promoted the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the shCD96 group was significantly inhibited compared to shNC group. (b) Western blotting showed that the level of p-Erk/Erk was significantly increased in the shCD96 group compared to the shNC group, but there was no significant difference observed in p-Akt/Akt. (c) Compared to the shNC group, the levels of TNF- α , IL-6, IL-17A, IFN- γ , IL-23, and IFN- γ were significantly increased in the cell culture supernatants of the shCD96 group ( ∗ P < 0.05, ∗∗ P < 0.01).

    Journal: BioMed Research International

    Article Title: CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

    doi: 10.1155/2022/3946754

    Figure Lengend Snippet: CD96 knockdown promoted the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the shCD96 group was significantly inhibited compared to shNC group. (b) Western blotting showed that the level of p-Erk/Erk was significantly increased in the shCD96 group compared to the shNC group, but there was no significant difference observed in p-Akt/Akt. (c) Compared to the shNC group, the levels of TNF- α , IL-6, IL-17A, IFN- γ , IL-23, and IFN- γ were significantly increased in the cell culture supernatants of the shCD96 group ( ∗ P < 0.05, ∗∗ P < 0.01).

    Article Snippet: The protein levels were detected using anti-CD96, anti-p-Akt, anti-Akt, anti-p-ERK, anti-ERK, and anti-GAPDH Abs and horseradish peroxidase- (HRP-) conjugated anti-mouse/rabbit IgG (Cell Signaling Technology, Beverly, MA, USA).

    Techniques: Expressing, Western Blot, Cell Culture

    CD96 overexpression alleviated the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the CD96 group was significantly increased compared to the vector group. (b) Western blotting showed that the level of p-Erk/Erk was significantly downregulated in the CD96 group compared to vector group, but no significant difference was detected in p-Akt/Akt. (c) Compared to the vector group, the levels of TNF- α , IL-6, IFN- γ , IL-17A, and IL-23 were significantly decreased in the cell culture supernatants of the CD96 group ( ∗ P < 0.05).

    Journal: BioMed Research International

    Article Title: CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

    doi: 10.1155/2022/3946754

    Figure Lengend Snippet: CD96 overexpression alleviated the inflammatory response of CD4 T cells (three independent replicates were performed). (a) qPCR showed that the expression of CD96 in the CD96 group was significantly increased compared to the vector group. (b) Western blotting showed that the level of p-Erk/Erk was significantly downregulated in the CD96 group compared to vector group, but no significant difference was detected in p-Akt/Akt. (c) Compared to the vector group, the levels of TNF- α , IL-6, IFN- γ , IL-17A, and IL-23 were significantly decreased in the cell culture supernatants of the CD96 group ( ∗ P < 0.05).

    Article Snippet: The protein levels were detected using anti-CD96, anti-p-Akt, anti-Akt, anti-p-ERK, anti-ERK, and anti-GAPDH Abs and horseradish peroxidase- (HRP-) conjugated anti-mouse/rabbit IgG (Cell Signaling Technology, Beverly, MA, USA).

    Techniques: Over Expression, Expressing, Plasmid Preparation, Western Blot, Cell Culture