jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc jak2
    HPNE cells were transfected with vector, Aurora A, <t>JAK2</t> or both Aurora A and JAK2 as described in Materials and Methods. Cells were harvested 48 h post-transfection, and the resulting lysates were immunoblotted with indicated antibodies (A) , processed for soft agar growth assays (B) or invasion assays (C) as described in Materials and Methods. White, light gray, dark gray and black colors represent vector, Aurora A (Aur), JAK2 and Aurora A + JAK2 (A+J), respectively. For the soft agar (B) , the data are the average +/− SE of three wells per condition. For the invasion (C), the data are the average +/− SE of three independent experiments. The data for (A) and (B) are representative of three independent experiments, respectively.
    Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jak2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jak2 - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Dual Aurora A and JAK2 kinase blockade effectively suppresses malignant transformation"

    Article Title: Dual Aurora A and JAK2 kinase blockade effectively suppresses malignant transformation

    Journal: Oncotarget

    doi:

    HPNE cells were transfected with vector, Aurora A, JAK2 or both Aurora A and JAK2 as described in Materials and Methods. Cells were harvested 48 h post-transfection, and the resulting lysates were immunoblotted with indicated antibodies (A) , processed for soft agar growth assays (B) or invasion assays (C) as described in Materials and Methods. White, light gray, dark gray and black colors represent vector, Aurora A (Aur), JAK2 and Aurora A + JAK2 (A+J), respectively. For the soft agar (B) , the data are the average +/− SE of three wells per condition. For the invasion (C), the data are the average +/− SE of three independent experiments. The data for (A) and (B) are representative of three independent experiments, respectively.
    Figure Legend Snippet: HPNE cells were transfected with vector, Aurora A, JAK2 or both Aurora A and JAK2 as described in Materials and Methods. Cells were harvested 48 h post-transfection, and the resulting lysates were immunoblotted with indicated antibodies (A) , processed for soft agar growth assays (B) or invasion assays (C) as described in Materials and Methods. White, light gray, dark gray and black colors represent vector, Aurora A (Aur), JAK2 and Aurora A + JAK2 (A+J), respectively. For the soft agar (B) , the data are the average +/− SE of three wells per condition. For the invasion (C), the data are the average +/− SE of three independent experiments. The data for (A) and (B) are representative of three independent experiments, respectively.

    Techniques Used: Transfection, Plasmid Preparation

    MDA-MB-468 and HT-29 cells were transfected with NT siRNA, Aurora A siRNA, JAK2 siRNA or siRNAs to both aurora A and JAK2, and processed for Western blotting (A) , soft agar (B) or invasion (C) assays as described under Materials and Methods. For the soft agar (B) , the data are the average +/− SE of three wells per condition. For the invasion (C), the data are the average +/− SE of three independent experiments. The data for (A) and (B) are representative of three independent experiments, respectively.
    Figure Legend Snippet: MDA-MB-468 and HT-29 cells were transfected with NT siRNA, Aurora A siRNA, JAK2 siRNA or siRNAs to both aurora A and JAK2, and processed for Western blotting (A) , soft agar (B) or invasion (C) assays as described under Materials and Methods. For the soft agar (B) , the data are the average +/− SE of three wells per condition. For the invasion (C), the data are the average +/− SE of three independent experiments. The data for (A) and (B) are representative of three independent experiments, respectively.

    Techniques Used: Transfection, Western Blot

    AJI-214 and AJI-100 synthesized as described by us previously and submitted to Reaction Biology Inc. for determination of IC50 values against Aurora A, Aurora B and JAK2 using the HotSpot method as described previously .
    Figure Legend Snippet: AJI-214 and AJI-100 synthesized as described by us previously and submitted to Reaction Biology Inc. for determination of IC50 values against Aurora A, Aurora B and JAK2 using the HotSpot method as described previously .

    Techniques Used: Synthesized