Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: C-Terminus of E1A Binding Protein 1 Stimulates Malignant Phenotype in Human Hepatocellular Carcinoma

doi: 10.12659/MSM.920114

Figure Lengend Snippet: Expression levels of CTBP1 and the clinicopathological characteristics of patients with HCC.

Article Snippet: The Western blotting process was performed as previously described [ ] and the PVDF membranes were incubated with the following primary antibodies at 4˚C overnight: rabbit anti-human CTBP1 (cat. no. 8684, Cell Signaling Technology), anti-human CTBP2 (cat. no. 13256, Cell Signaling Technology), anti-human phospho-Akt at Thr308 site (cat. no. 13038, Cell Signaling Technology), anti-human Akt (cat. no. 2920, Cell Signaling Technology), anti-human phospho-phosphatidylinositol-3-kinase p110α (cat. no. 4255, Cell Signaling Technology), and mouse anti-human β-actin (cat. no. ab 8227, Abcam).

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: C-Terminus of E1A Binding Protein 1 Stimulates Malignant Phenotype in Human Hepatocellular Carcinoma

doi: 10.12659/MSM.920114

Figure Lengend Snippet: CTBP1 was highly expressed in HCC tissues. ( A ) High CTBP1 expression in HCC tissues was observed via IHC. ( B ) The protein of CTBP1 was highly expressed in HCC tissues. ** P<0.01 vs. hepatic tissues. ( C ) Positive CTBP1 expression predicted a shorter survival time. HCC – hepatocellular carcinoma; CTBP – C-terminus of the E1A binding proteins.

Article Snippet: The Western blotting process was performed as previously described [ ] and the PVDF membranes were incubated with the following primary antibodies at 4˚C overnight: rabbit anti-human CTBP1 (cat. no. 8684, Cell Signaling Technology), anti-human CTBP2 (cat. no. 13256, Cell Signaling Technology), anti-human phospho-Akt at Thr308 site (cat. no. 13038, Cell Signaling Technology), anti-human Akt (cat. no. 2920, Cell Signaling Technology), anti-human phospho-phosphatidylinositol-3-kinase p110α (cat. no. 4255, Cell Signaling Technology), and mouse anti-human β-actin (cat. no. ab 8227, Abcam).

Techniques: Expressing, Binding Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: C-Terminus of E1A Binding Protein 1 Stimulates Malignant Phenotype in Human Hepatocellular Carcinoma

doi: 10.12659/MSM.920114

Figure Lengend Snippet: CTBP1 promoted the malignancy of hepatic astrocytes. ( A ) Modifications in activation of the PI3K/Akt pathway. ( B ) Growth curve of the LX-2 cells as determined via the CCK-8. ( C ) Colony formation activity in 2D monolayer cultures. ( D ) The in vitro invasive activity of LX-2 cells was detected by the Transwell chamber method. ( E ) The in vitro migratory activity of LX-2 cells was investigated using the cell scratch assay. ** P<0.01 vs. the vector group. CTBP – C-terminus of the E1A binding proteins.

Article Snippet: The Western blotting process was performed as previously described [ ] and the PVDF membranes were incubated with the following primary antibodies at 4˚C overnight: rabbit anti-human CTBP1 (cat. no. 8684, Cell Signaling Technology), anti-human CTBP2 (cat. no. 13256, Cell Signaling Technology), anti-human phospho-Akt at Thr308 site (cat. no. 13038, Cell Signaling Technology), anti-human Akt (cat. no. 2920, Cell Signaling Technology), anti-human phospho-phosphatidylinositol-3-kinase p110α (cat. no. 4255, Cell Signaling Technology), and mouse anti-human β-actin (cat. no. ab 8227, Abcam).

Techniques: Activation Assay, CCK-8 Assay, Activity Assay, In Vitro, Wound Healing Assay, Plasmid Preparation, Binding Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: C-Terminus of E1A Binding Protein 1 Stimulates Malignant Phenotype in Human Hepatocellular Carcinoma

doi: 10.12659/MSM.920114

Figure Lengend Snippet: CTBP1 silencing reduced the migratory activity of HCC cells. ( A ) The consequences of CTBP1 loss on the PI3K/Akt signaling in Hep3B cells. ( B ) The growth rate of Hep3B cells was assessed via CCK-8 assay. ( C ) The colony formation activity of Hep3B cells was evaluated using 2D culture. ( D ) The in vitro invasive activity of Hep3B cells. ( E ) The in vitro migratory activity of Hep3B cells. ** P<0.01, vs. the scramble group.

Article Snippet: The Western blotting process was performed as previously described [ ] and the PVDF membranes were incubated with the following primary antibodies at 4˚C overnight: rabbit anti-human CTBP1 (cat. no. 8684, Cell Signaling Technology), anti-human CTBP2 (cat. no. 13256, Cell Signaling Technology), anti-human phospho-Akt at Thr308 site (cat. no. 13038, Cell Signaling Technology), anti-human Akt (cat. no. 2920, Cell Signaling Technology), anti-human phospho-phosphatidylinositol-3-kinase p110α (cat. no. 4255, Cell Signaling Technology), and mouse anti-human β-actin (cat. no. ab 8227, Abcam).

Techniques: Activity Assay, CCK-8 Assay, In Vitro