primary antibodies against ptbc1d4 ser318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies against ptbc1d4 ser318
    Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), <t>pTBC1D4</t> <t>Ser318</t> ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).
    Primary Antibodies Against Ptbc1d4 Ser318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against ptbc1d4 ser318/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against ptbc1d4 ser318 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle"

    Article Title: Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle

    Journal: Diabetes

    doi: 10.2337/db21-0855

    Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).
    Figure Legend Snippet: Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).

    Techniques Used: Western Blot

    Insulin-stimulated regulation of the TBC1D1-TBC1D4 complex in skeletal muscle from healthy, lean individuals and obese individuals with type 2 diabetes. IP of TBC1D1 from basal and insulin-stimulated human skeletal muscle in healthy, lean individuals and obese individuals with type 2 diabetes, followed by Western blot of p-TBC1D4 Ser318 ( A ), p-TBC1D4 Thr642 ( B ), TBC1D1 total ( C ) and TBC1D4 ( D ). E : Ratio of TBC1D4 co-IP to TBC1D1 IP. Representative Western blot of IP data. Bars are means and lines represent individual responses ( n = 10). Data were analyzed by two-way repeated-measures ANOVA within group. ANOVA significance is displayed with text on each graph. Šidák multiple comparisons test was used as post hoc test. **** P < 0.0001, ** P < 0.01, and * P < 0.05.
    Figure Legend Snippet: Insulin-stimulated regulation of the TBC1D1-TBC1D4 complex in skeletal muscle from healthy, lean individuals and obese individuals with type 2 diabetes. IP of TBC1D1 from basal and insulin-stimulated human skeletal muscle in healthy, lean individuals and obese individuals with type 2 diabetes, followed by Western blot of p-TBC1D4 Ser318 ( A ), p-TBC1D4 Thr642 ( B ), TBC1D1 total ( C ) and TBC1D4 ( D ). E : Ratio of TBC1D4 co-IP to TBC1D1 IP. Representative Western blot of IP data. Bars are means and lines represent individual responses ( n = 10). Data were analyzed by two-way repeated-measures ANOVA within group. ANOVA significance is displayed with text on each graph. Šidák multiple comparisons test was used as post hoc test. **** P < 0.0001, ** P < 0.01, and * P < 0.05.

    Techniques Used: Western Blot, Co-Immunoprecipitation Assay

    Intact insulin- and exercise-mediated phosphorylation of TBC1D4 in complex with TBC1D1. IP of TBC1D1 from basal ( B ) and insulin (I)-stimulated human skeletal muscle, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), and pTBC1D4 Thr642 ( C ). IP of TBC1D1 from rested (R) and acutely exercised ( E ) human skeletal muscle, followed by Western blot of TBC1D4 total ( D ), pTBC1D4 Ser318 ( E ), and pTBC1D4 Thr642 ( F ). G : Representative Western blot of IP data. Input refers to muscle lysate prior to IP. Dots and lines represent individual responses ( n = 11 in A – C and n = 8 D-F). Data were analyzed by paired Student t test. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05.
    Figure Legend Snippet: Intact insulin- and exercise-mediated phosphorylation of TBC1D4 in complex with TBC1D1. IP of TBC1D1 from basal ( B ) and insulin (I)-stimulated human skeletal muscle, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), and pTBC1D4 Thr642 ( C ). IP of TBC1D1 from rested (R) and acutely exercised ( E ) human skeletal muscle, followed by Western blot of TBC1D4 total ( D ), pTBC1D4 Ser318 ( E ), and pTBC1D4 Thr642 ( F ). G : Representative Western blot of IP data. Input refers to muscle lysate prior to IP. Dots and lines represent individual responses ( n = 11 in A – C and n = 8 D-F). Data were analyzed by paired Student t test. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05.

    Techniques Used: Western Blot

    primary antibodies against ptbc1d4 ser318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies against ptbc1d4 ser318
    Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), <t>pTBC1D4</t> <t>Ser318</t> ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).
    Primary Antibodies Against Ptbc1d4 Ser318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against ptbc1d4 ser318/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against ptbc1d4 ser318 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle"

    Article Title: Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle

    Journal: Diabetes

    doi: 10.2337/db21-0855

    Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).
    Figure Legend Snippet: Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).

    Techniques Used: Western Blot

    Insulin-stimulated regulation of the TBC1D1-TBC1D4 complex in skeletal muscle from healthy, lean individuals and obese individuals with type 2 diabetes. IP of TBC1D1 from basal and insulin-stimulated human skeletal muscle in healthy, lean individuals and obese individuals with type 2 diabetes, followed by Western blot of p-TBC1D4 Ser318 ( A ), p-TBC1D4 Thr642 ( B ), TBC1D1 total ( C ) and TBC1D4 ( D ). E : Ratio of TBC1D4 co-IP to TBC1D1 IP. Representative Western blot of IP data. Bars are means and lines represent individual responses ( n = 10). Data were analyzed by two-way repeated-measures ANOVA within group. ANOVA significance is displayed with text on each graph. Šidák multiple comparisons test was used as post hoc test. **** P < 0.0001, ** P < 0.01, and * P < 0.05.
    Figure Legend Snippet: Insulin-stimulated regulation of the TBC1D1-TBC1D4 complex in skeletal muscle from healthy, lean individuals and obese individuals with type 2 diabetes. IP of TBC1D1 from basal and insulin-stimulated human skeletal muscle in healthy, lean individuals and obese individuals with type 2 diabetes, followed by Western blot of p-TBC1D4 Ser318 ( A ), p-TBC1D4 Thr642 ( B ), TBC1D1 total ( C ) and TBC1D4 ( D ). E : Ratio of TBC1D4 co-IP to TBC1D1 IP. Representative Western blot of IP data. Bars are means and lines represent individual responses ( n = 10). Data were analyzed by two-way repeated-measures ANOVA within group. ANOVA significance is displayed with text on each graph. Šidák multiple comparisons test was used as post hoc test. **** P < 0.0001, ** P < 0.01, and * P < 0.05.

    Techniques Used: Western Blot, Co-Immunoprecipitation Assay

    Intact insulin- and exercise-mediated phosphorylation of TBC1D4 in complex with TBC1D1. IP of TBC1D1 from basal ( B ) and insulin (I)-stimulated human skeletal muscle, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), and pTBC1D4 Thr642 ( C ). IP of TBC1D1 from rested (R) and acutely exercised ( E ) human skeletal muscle, followed by Western blot of TBC1D4 total ( D ), pTBC1D4 Ser318 ( E ), and pTBC1D4 Thr642 ( F ). G : Representative Western blot of IP data. Input refers to muscle lysate prior to IP. Dots and lines represent individual responses ( n = 11 in A – C and n = 8 D-F). Data were analyzed by paired Student t test. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05.
    Figure Legend Snippet: Intact insulin- and exercise-mediated phosphorylation of TBC1D4 in complex with TBC1D1. IP of TBC1D1 from basal ( B ) and insulin (I)-stimulated human skeletal muscle, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), and pTBC1D4 Thr642 ( C ). IP of TBC1D1 from rested (R) and acutely exercised ( E ) human skeletal muscle, followed by Western blot of TBC1D4 total ( D ), pTBC1D4 Ser318 ( E ), and pTBC1D4 Thr642 ( F ). G : Representative Western blot of IP data. Input refers to muscle lysate prior to IP. Dots and lines represent individual responses ( n = 11 in A – C and n = 8 D-F). Data were analyzed by paired Student t test. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05.

    Techniques Used: Western Blot

    anti p as160  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p as160
    The RT‐qPCR primer sequences (Mouse)
    Anti P As160, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94/100 stars

    Images

    1) Product Images from "Berberine regulates mesangial cell proliferation and cell cycle to attenuate diabetic nephropathy through the PI3K/Akt/AS160/GLUT1 signalling pathway"

    Article Title: Berberine regulates mesangial cell proliferation and cell cycle to attenuate diabetic nephropathy through the PI3K/Akt/AS160/GLUT1 signalling pathway

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.17167

    The RT‐qPCR primer sequences (Mouse)
    Figure Legend Snippet: The RT‐qPCR primer sequences (Mouse)

    Techniques Used:

    Effect of BBR on regulating the PI3K/Akt/AS160/GLUT1 pathway. Protein was extracted from cultured GMCs. GAPDH was used as internal controls. Intensity of PI3K, AKT, p‐AKT, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 was quantified, normalized to internal control and expressed as mean ± SEM. (A,B) The levels of PI3K, Akt, p‐Akt, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 were detected in each group; (C) RT‐qPCR analyses were performed to detect the mRNA expression of PI3K, Akt, AS160 and GLUT1 in each group. Relative expression of PI3K, Akt, AS160 and GLUT1. Data are expressed as Mean ± SEM, n = 3. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HG group. HG: high glucose; BBR: HG + BBR (60 μmol/L); LY294002 (HG + LY294002); Metformin: HG + metformin (5 mmol/L)
    Figure Legend Snippet: Effect of BBR on regulating the PI3K/Akt/AS160/GLUT1 pathway. Protein was extracted from cultured GMCs. GAPDH was used as internal controls. Intensity of PI3K, AKT, p‐AKT, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 was quantified, normalized to internal control and expressed as mean ± SEM. (A,B) The levels of PI3K, Akt, p‐Akt, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 were detected in each group; (C) RT‐qPCR analyses were performed to detect the mRNA expression of PI3K, Akt, AS160 and GLUT1 in each group. Relative expression of PI3K, Akt, AS160 and GLUT1. Data are expressed as Mean ± SEM, n = 3. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HG group. HG: high glucose; BBR: HG + BBR (60 μmol/L); LY294002 (HG + LY294002); Metformin: HG + metformin (5 mmol/L)

    Techniques Used: Cell Culture, Quantitative RT-PCR, Expressing

    anti pas160 s318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti pas160 s318
    Anti Pas160 S318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pas160 s318/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    anti pas160 s318 - by Bioz Stars, 2023-02
    94/100 stars

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    as160ser318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc as160ser318
    As160ser318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/as160ser318/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    rabbit polyclonal anti phosphorylated as160 ser 318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti phosphorylated as160 ser 318
    Rabbit Polyclonal Anti Phosphorylated As160 Ser 318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phospho as160 8619 antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho as160 8619 antibodies
    Anti Phospho As160 8619 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    de upstate anti tbc1d4 ser318 phosphorylation cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc de upstate anti tbc1d4 ser318 phosphorylation cell signaling technology
    De Upstate Anti Tbc1d4 Ser318 Phosphorylation Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/de upstate anti tbc1d4 ser318 phosphorylation cell signaling technology/product/Cell Signaling Technology Inc
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    de upstate anti tbc1d4 ser318 phosphorylation cell signaling technology - by Bioz Stars, 2023-02
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    phospho as160 ser318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho as160 ser318
    (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated <t>AS160</t> (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.
    Phospho As160 Ser318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho as160 ser318/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    phospho as160 ser318 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Lysosome Positioning Influences mTORC2 and AKT Signaling"

    Article Title: Lysosome Positioning Influences mTORC2 and AKT Signaling

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2019.05.009

    (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated AS160 (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.
    Figure Legend Snippet: (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated AS160 (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.

    Techniques Used: Transfection, Incubation, SDS Page, Western Blot, Serum Depletion

    Key Resources Table
    Figure Legend Snippet: Key Resources Table

    Techniques Used: Recombinant, Electron Microscopy, Protease Inhibitor, Software, Modification, Transfection, Magnetic Beads

    cell signaling merck millipore cell signaling cell signaling cell signaling 8619  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signaling merck millipore cell signaling cell signaling cell signaling 8619
    Cell Signaling Merck Millipore Cell Signaling Cell Signaling Cell Signaling 8619, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell signaling merck millipore cell signaling cell signaling cell signaling 8619/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    cell signaling merck millipore cell signaling cell signaling cell signaling 8619 - by Bioz Stars, 2023-02
    94/100 stars

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    tbc1d4 phosphorylation at ser318  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tbc1d4 phosphorylation at ser318
    (a–c) Muscle Akt pSer308: Akt2 protein (a), and Akt pThr473: Akt2 protein (b), TBC1D1 pSer237: TBC1D1 protein (c), TBC1D1 pSer700: TBC1D1 protein (d), <t>TBC1D4</t> pSer318: TBC1D4 protein (e), TBC1D4 pSer588: TBC1D4 protein (f) and TBC1D4 pThr642: TBC1D4 protein (g) in the short‐term study. Data are means ± SEM . Black bars shows results for the metformin trial. White bars shows results for the placebo trial. Representative immunoblots are shown in figure (h). ∗ p < .05 and ∗∗∗ p < .001 versus “Pre”; ††† p < .001 versus preceding value
    Tbc1d4 Phosphorylation At Ser318, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Metformin does not compromise energy status in human skeletal muscle at rest or during acute exercise: A randomised, crossover trial"

    Article Title: Metformin does not compromise energy status in human skeletal muscle at rest or during acute exercise: A randomised, crossover trial

    Journal: Physiological Reports

    doi: 10.14814/phy2.14307

    (a–c) Muscle Akt pSer308: Akt2 protein (a), and Akt pThr473: Akt2 protein (b), TBC1D1 pSer237: TBC1D1 protein (c), TBC1D1 pSer700: TBC1D1 protein (d), TBC1D4 pSer318: TBC1D4 protein (e), TBC1D4 pSer588: TBC1D4 protein (f) and TBC1D4 pThr642: TBC1D4 protein (g) in the short‐term study. Data are means ± SEM . Black bars shows results for the metformin trial. White bars shows results for the placebo trial. Representative immunoblots are shown in figure (h). ∗ p < .05 and ∗∗∗ p < .001 versus “Pre”; ††† p < .001 versus preceding value
    Figure Legend Snippet: (a–c) Muscle Akt pSer308: Akt2 protein (a), and Akt pThr473: Akt2 protein (b), TBC1D1 pSer237: TBC1D1 protein (c), TBC1D1 pSer700: TBC1D1 protein (d), TBC1D4 pSer318: TBC1D4 protein (e), TBC1D4 pSer588: TBC1D4 protein (f) and TBC1D4 pThr642: TBC1D4 protein (g) in the short‐term study. Data are means ± SEM . Black bars shows results for the metformin trial. White bars shows results for the placebo trial. Representative immunoblots are shown in figure (h). ∗ p < .05 and ∗∗∗ p < .001 versus “Pre”; ††† p < .001 versus preceding value

    Techniques Used: Western Blot

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    Cell Signaling Technology Inc primary antibodies against ptbc1d4 ser318
    Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), <t>pTBC1D4</t> <t>Ser318</t> ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).
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    Cell Signaling Technology Inc phospho as160 ser318
    (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated <t>AS160</t> (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.
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    (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated <t>AS160</t> (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.
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    Cell Signaling Technology Inc tbc1d4 phosphorylation at ser318
    (a–c) Muscle Akt pSer308: Akt2 protein (a), and Akt pThr473: Akt2 protein (b), TBC1D1 pSer237: TBC1D1 protein (c), TBC1D1 pSer700: TBC1D1 protein (d), <t>TBC1D4</t> pSer318: TBC1D4 protein (e), TBC1D4 pSer588: TBC1D4 protein (f) and TBC1D4 pThr642: TBC1D4 protein (g) in the short‐term study. Data are means ± SEM . Black bars shows results for the metformin trial. White bars shows results for the placebo trial. Representative immunoblots are shown in figure (h). ∗ p < .05 and ∗∗∗ p < .001 versus “Pre”; ††† p < .001 versus preceding value
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    Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).

    Journal: Diabetes

    Article Title: Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle

    doi: 10.2337/db21-0855

    Figure Lengend Snippet: Insulin-stimulated phosphorylation of the TBC1D1-TBC1D4 complex after exercise. IP of TBC1D1 from rested ( R ) and prior exercised ( E ) human skeletal muscle at basal ( B ) and in response to insulin ( I ) stimulation, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), pTBC1D4 Thr642 ( C ), and pTBC1D4 Ser704 ( D ). E : Representative Western blot of IP data. Data were analyzed by repeated-measures ANOVA. ANOVA significance is displayed with text on each graph. Values for each individual are marked with a unique asterisk and connected with lines within each leg ( n = 7).

    Article Snippet: Primary antibodies against pTBC1D4-Ser318 (8619), pTBC1D4-Thr642 (8881), pTBC1D1-Thr596 (6927S), pAkt-Thr308 (9275), pAkt-Ser473 (4271L), Akt2 (3063), pAMPK-Thr172 (2531), and GAPDH (2118) were from Cell Signaling Technology.

    Techniques: Western Blot

    Insulin-stimulated regulation of the TBC1D1-TBC1D4 complex in skeletal muscle from healthy, lean individuals and obese individuals with type 2 diabetes. IP of TBC1D1 from basal and insulin-stimulated human skeletal muscle in healthy, lean individuals and obese individuals with type 2 diabetes, followed by Western blot of p-TBC1D4 Ser318 ( A ), p-TBC1D4 Thr642 ( B ), TBC1D1 total ( C ) and TBC1D4 ( D ). E : Ratio of TBC1D4 co-IP to TBC1D1 IP. Representative Western blot of IP data. Bars are means and lines represent individual responses ( n = 10). Data were analyzed by two-way repeated-measures ANOVA within group. ANOVA significance is displayed with text on each graph. Šidák multiple comparisons test was used as post hoc test. **** P < 0.0001, ** P < 0.01, and * P < 0.05.

    Journal: Diabetes

    Article Title: Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle

    doi: 10.2337/db21-0855

    Figure Lengend Snippet: Insulin-stimulated regulation of the TBC1D1-TBC1D4 complex in skeletal muscle from healthy, lean individuals and obese individuals with type 2 diabetes. IP of TBC1D1 from basal and insulin-stimulated human skeletal muscle in healthy, lean individuals and obese individuals with type 2 diabetes, followed by Western blot of p-TBC1D4 Ser318 ( A ), p-TBC1D4 Thr642 ( B ), TBC1D1 total ( C ) and TBC1D4 ( D ). E : Ratio of TBC1D4 co-IP to TBC1D1 IP. Representative Western blot of IP data. Bars are means and lines represent individual responses ( n = 10). Data were analyzed by two-way repeated-measures ANOVA within group. ANOVA significance is displayed with text on each graph. Šidák multiple comparisons test was used as post hoc test. **** P < 0.0001, ** P < 0.01, and * P < 0.05.

    Article Snippet: Primary antibodies against pTBC1D4-Ser318 (8619), pTBC1D4-Thr642 (8881), pTBC1D1-Thr596 (6927S), pAkt-Thr308 (9275), pAkt-Ser473 (4271L), Akt2 (3063), pAMPK-Thr172 (2531), and GAPDH (2118) were from Cell Signaling Technology.

    Techniques: Western Blot, Co-Immunoprecipitation Assay

    Intact insulin- and exercise-mediated phosphorylation of TBC1D4 in complex with TBC1D1. IP of TBC1D1 from basal ( B ) and insulin (I)-stimulated human skeletal muscle, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), and pTBC1D4 Thr642 ( C ). IP of TBC1D1 from rested (R) and acutely exercised ( E ) human skeletal muscle, followed by Western blot of TBC1D4 total ( D ), pTBC1D4 Ser318 ( E ), and pTBC1D4 Thr642 ( F ). G : Representative Western blot of IP data. Input refers to muscle lysate prior to IP. Dots and lines represent individual responses ( n = 11 in A – C and n = 8 D-F). Data were analyzed by paired Student t test. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05.

    Journal: Diabetes

    Article Title: Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle

    doi: 10.2337/db21-0855

    Figure Lengend Snippet: Intact insulin- and exercise-mediated phosphorylation of TBC1D4 in complex with TBC1D1. IP of TBC1D1 from basal ( B ) and insulin (I)-stimulated human skeletal muscle, followed by Western blot of TBC1D4 total ( A ), pTBC1D4 Ser318 ( B ), and pTBC1D4 Thr642 ( C ). IP of TBC1D1 from rested (R) and acutely exercised ( E ) human skeletal muscle, followed by Western blot of TBC1D4 total ( D ), pTBC1D4 Ser318 ( E ), and pTBC1D4 Thr642 ( F ). G : Representative Western blot of IP data. Input refers to muscle lysate prior to IP. Dots and lines represent individual responses ( n = 11 in A – C and n = 8 D-F). Data were analyzed by paired Student t test. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05.

    Article Snippet: Primary antibodies against pTBC1D4-Ser318 (8619), pTBC1D4-Thr642 (8881), pTBC1D1-Thr596 (6927S), pAkt-Thr308 (9275), pAkt-Ser473 (4271L), Akt2 (3063), pAMPK-Thr172 (2531), and GAPDH (2118) were from Cell Signaling Technology.

    Techniques: Western Blot

    The RT‐qPCR primer sequences (Mouse)

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Berberine regulates mesangial cell proliferation and cell cycle to attenuate diabetic nephropathy through the PI3K/Akt/AS160/GLUT1 signalling pathway

    doi: 10.1111/jcmm.17167

    Figure Lengend Snippet: The RT‐qPCR primer sequences (Mouse)

    Article Snippet: Anti‐PI3K‐p85 (#4292), anti‐Akt (4691S), anti‐p‐Akt (#4060), anti‐AS160 (#2670), anti‐p‐AS160 (#8619) and anti‐GAPDH (#5174) antibodies were obtained from Cell Signaling Biotechnology.

    Techniques:

    Effect of BBR on regulating the PI3K/Akt/AS160/GLUT1 pathway. Protein was extracted from cultured GMCs. GAPDH was used as internal controls. Intensity of PI3K, AKT, p‐AKT, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 was quantified, normalized to internal control and expressed as mean ± SEM. (A,B) The levels of PI3K, Akt, p‐Akt, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 were detected in each group; (C) RT‐qPCR analyses were performed to detect the mRNA expression of PI3K, Akt, AS160 and GLUT1 in each group. Relative expression of PI3K, Akt, AS160 and GLUT1. Data are expressed as Mean ± SEM, n = 3. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HG group. HG: high glucose; BBR: HG + BBR (60 μmol/L); LY294002 (HG + LY294002); Metformin: HG + metformin (5 mmol/L)

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Berberine regulates mesangial cell proliferation and cell cycle to attenuate diabetic nephropathy through the PI3K/Akt/AS160/GLUT1 signalling pathway

    doi: 10.1111/jcmm.17167

    Figure Lengend Snippet: Effect of BBR on regulating the PI3K/Akt/AS160/GLUT1 pathway. Protein was extracted from cultured GMCs. GAPDH was used as internal controls. Intensity of PI3K, AKT, p‐AKT, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 was quantified, normalized to internal control and expressed as mean ± SEM. (A,B) The levels of PI3K, Akt, p‐Akt, AS160, p‐AS160, Total GLUT1 and m‐GLUT1 were detected in each group; (C) RT‐qPCR analyses were performed to detect the mRNA expression of PI3K, Akt, AS160 and GLUT1 in each group. Relative expression of PI3K, Akt, AS160 and GLUT1. Data are expressed as Mean ± SEM, n = 3. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HG group. HG: high glucose; BBR: HG + BBR (60 μmol/L); LY294002 (HG + LY294002); Metformin: HG + metformin (5 mmol/L)

    Article Snippet: Anti‐PI3K‐p85 (#4292), anti‐Akt (4691S), anti‐p‐Akt (#4060), anti‐AS160 (#2670), anti‐p‐AS160 (#8619) and anti‐GAPDH (#5174) antibodies were obtained from Cell Signaling Biotechnology.

    Techniques: Cell Culture, Quantitative RT-PCR, Expressing

    (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated AS160 (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.

    Journal: Molecular cell

    Article Title: Lysosome Positioning Influences mTORC2 and AKT Signaling

    doi: 10.1016/j.molcel.2019.05.009

    Figure Lengend Snippet: (A) WT, myrlysin-KO, myrlysin-rescue (myrlysin-KO transfected with myrlysin cDNA), diaskedin-KO, lyspersin-KO and KIF1B-KIF5B-KO HeLa cells were incubated in medium depleted of both amino acids and serum for 30 min, or serum for 1 h, and then refed with the corresponding nutrients for the indicated periods. Cell lysates were subjected to SDS-PAGE and immunoblotting with antibodies to phosphorylated TSC2 (p-TSC2), and total TSC2 (t-TSC2). Arrowheads indicate the specific p-TSC2 bands. (B) Quantification of the ratio of p-TSC2 to t-TSC2 upon combined amino-acid and serum depletion-refeeding. (C) Quantification of the ratio of p-TSC2 to t-TSC2 upon serum depletion-refeeding. The line graphs for WT and myrlysin-KO cells are the same shown in Fig. 3D. In B and C, values are the mean ± SEM from three independent experiments such as that shown in A. *p<0.01, **p<0.001, ***p<0.0001 (WT vs. myrlysin KO), two-way ANOVA with Tukey’s multiple comparisons test. (D) WT and myrlysin-KO HeLa cells were subjected to serum starvation and refeeding. Cell lysates were analyzed as in A for phosphorylated p-TSC2, t-TSC2, phosphorylated FoxO3 (p-FoxO3), total FoxO3 (t-FoxO3), phosphorylated AS160 (p-AS160), total AS160 (t-AS160), phosphorylated GSK-3β (p- GSK-3β), total GSK-β3 (p-GSK-3β), S473-phosphorylated AKT [p-AKT (S473)] and total AKT (t-AKT). In A and E, the positions of molecular mass markers (in kDa) are indicated on the left.

    Article Snippet: Phospho-AS160 (Ser318) , Cell Signaling Technology , 8619.

    Techniques: Transfection, Incubation, SDS Page, Western Blot, Serum Depletion

    Key Resources Table

    Journal: Molecular cell

    Article Title: Lysosome Positioning Influences mTORC2 and AKT Signaling

    doi: 10.1016/j.molcel.2019.05.009

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: Phospho-AS160 (Ser318) , Cell Signaling Technology , 8619.

    Techniques: Recombinant, Electron Microscopy, Protease Inhibitor, Software, Modification, Transfection, Magnetic Beads

    (a–c) Muscle Akt pSer308: Akt2 protein (a), and Akt pThr473: Akt2 protein (b), TBC1D1 pSer237: TBC1D1 protein (c), TBC1D1 pSer700: TBC1D1 protein (d), TBC1D4 pSer318: TBC1D4 protein (e), TBC1D4 pSer588: TBC1D4 protein (f) and TBC1D4 pThr642: TBC1D4 protein (g) in the short‐term study. Data are means ± SEM . Black bars shows results for the metformin trial. White bars shows results for the placebo trial. Representative immunoblots are shown in figure (h). ∗ p < .05 and ∗∗∗ p < .001 versus “Pre”; ††† p < .001 versus preceding value

    Journal: Physiological Reports

    Article Title: Metformin does not compromise energy status in human skeletal muscle at rest or during acute exercise: A randomised, crossover trial

    doi: 10.14814/phy2.14307

    Figure Lengend Snippet: (a–c) Muscle Akt pSer308: Akt2 protein (a), and Akt pThr473: Akt2 protein (b), TBC1D1 pSer237: TBC1D1 protein (c), TBC1D1 pSer700: TBC1D1 protein (d), TBC1D4 pSer318: TBC1D4 protein (e), TBC1D4 pSer588: TBC1D4 protein (f) and TBC1D4 pThr642: TBC1D4 protein (g) in the short‐term study. Data are means ± SEM . Black bars shows results for the metformin trial. White bars shows results for the placebo trial. Representative immunoblots are shown in figure (h). ∗ p < .05 and ∗∗∗ p < .001 versus “Pre”; ††† p < .001 versus preceding value

    Article Snippet: The ACC phosphospecific antibody is raised against a peptide corresponding to the sequence in rat ACC1 containing the Ser79 phosphorylation site, but the antibody also recognized the human ACC1 and ACC2 when phosphorylated most likely at the corresponding Ser80 and Ser221, respectively (Abu‐Elheiga, Almarza‐Ortega, Baldini & Wakil, ); ACC protein content (Dako, Streptavidin/HRP P0397); Akt2 protein content (Cell Signalling Technology Inc, #3063); Akt phosphorylation at Thr308 (Cell Signalling Technology Inc, #9275); Akt phosphorylation at Ser473 (Cell Signalling Technology Inc, #9271); TBC1D1 protein (provided by Prof Hardie DG, University of Dundee, UK); TBC1D1 phosphorylation at Ser237 (Millipore, #2061452); TBC1D1 phosphorylation at Ser700 – (Cell Signalling Technology Inc, #9271); TBC1D4 protein content (Abcam, #24469); TBC1D4 phosphorylation at Ser318 (Cell Signalling Technology Inc, #8619); TBC1D4 phosphorylation at Ser588 (Cell Signalling Technology Inc, #8730); TBC1D4 phosphorylation at Thr642 (Symansis, NZ #3028 P1).

    Techniques: Western Blot