rabbit anti bag6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti bag6
    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and <t>BAG6</t> binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.
    Rabbit Anti Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase"

    Article Title: VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

    Journal: BMC Biology

    doi: 10.1186/1741-7007-12-39

    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and BAG6 binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.
    Figure Legend Snippet: VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and BAG6 binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.

    Techniques Used: Inhibition, Binding Assay, Expressing, Immunoprecipitation, Mutagenesis, Luciferase

    TRC subunits were identified by mass spectrometry in Flag-FAF1 immunoprecipitates
    Figure Legend Snippet: TRC subunits were identified by mass spectrometry in Flag-FAF1 immunoprecipitates

    Techniques Used: Mass Spectrometry

    TRC complex subunits slightly accumulate in endogenous VAPB immunoprecipitates upon proteasome inhibition
    Figure Legend Snippet: TRC complex subunits slightly accumulate in endogenous VAPB immunoprecipitates upon proteasome inhibition

    Techniques Used:

    ASNA1 interacts with VAPB via a FFAT-like motif similar to FAF1. (A) Alignment of the FFAT-like motifs in human ASNA1 and FAF1. (B) Alignment of the FFAT-like motif of ASNA1 across species showing that it is highly conserved. (C) A point mutation in the FFAT-like motif of ASNA1 (F15A) abolishes its interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from U2OS cells using anti-Flag beads. (D) Indirect immunofluorescence of VAPB and Flag-ASNA1 WT. U2OS cells were transfected with Flag-ASNA1 WT for 24 hr. Flag-ASNA1 WT (red) is co-localized with VAPB (green) in a peri-nuclear area (enlarged window) suggesting an ER pattern. Scale bar is 10 μm. (E) ASNA1 interaction with FAF1 is strongly stimulated upon proteasome inhibition with MG132 and depends on the UBA domain. WT Flag-FAF1 and the indicated mutants were immunoprecipitated from U2OS cells. (F) G46R and G46A point mutations in ASNA1 abolish its interaction with BAG6 and strongly reduce its interaction with FAF1 and ubiquitin, most noticeably after MG132 treatment, but do not affect the interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from SH-SY5Y cells. DAPI, 4',6-diamidino-2-phenylindole; IP, immunoprecipitate; WT, wild type.
    Figure Legend Snippet: ASNA1 interacts with VAPB via a FFAT-like motif similar to FAF1. (A) Alignment of the FFAT-like motifs in human ASNA1 and FAF1. (B) Alignment of the FFAT-like motif of ASNA1 across species showing that it is highly conserved. (C) A point mutation in the FFAT-like motif of ASNA1 (F15A) abolishes its interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from U2OS cells using anti-Flag beads. (D) Indirect immunofluorescence of VAPB and Flag-ASNA1 WT. U2OS cells were transfected with Flag-ASNA1 WT for 24 hr. Flag-ASNA1 WT (red) is co-localized with VAPB (green) in a peri-nuclear area (enlarged window) suggesting an ER pattern. Scale bar is 10 μm. (E) ASNA1 interaction with FAF1 is strongly stimulated upon proteasome inhibition with MG132 and depends on the UBA domain. WT Flag-FAF1 and the indicated mutants were immunoprecipitated from U2OS cells. (F) G46R and G46A point mutations in ASNA1 abolish its interaction with BAG6 and strongly reduce its interaction with FAF1 and ubiquitin, most noticeably after MG132 treatment, but do not affect the interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from SH-SY5Y cells. DAPI, 4',6-diamidino-2-phenylindole; IP, immunoprecipitate; WT, wild type.

    Techniques Used: Mutagenesis, Immunoprecipitation, Immunofluorescence, Transfection, Inhibition

    rabbit anti bag6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti bag6
    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and <t>BAG6</t> binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.
    Rabbit Anti Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase"

    Article Title: VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

    Journal: BMC Biology

    doi: 10.1186/1741-7007-12-39

    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and BAG6 binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.
    Figure Legend Snippet: VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and BAG6 binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.

    Techniques Used: Inhibition, Binding Assay, Expressing, Immunoprecipitation, Mutagenesis, Luciferase

    TRC subunits were identified by mass spectrometry in Flag-FAF1 immunoprecipitates
    Figure Legend Snippet: TRC subunits were identified by mass spectrometry in Flag-FAF1 immunoprecipitates

    Techniques Used: Mass Spectrometry

    TRC complex subunits slightly accumulate in endogenous VAPB immunoprecipitates upon proteasome inhibition
    Figure Legend Snippet: TRC complex subunits slightly accumulate in endogenous VAPB immunoprecipitates upon proteasome inhibition

    Techniques Used:

    ASNA1 interacts with VAPB via a FFAT-like motif similar to FAF1. (A) Alignment of the FFAT-like motifs in human ASNA1 and FAF1. (B) Alignment of the FFAT-like motif of ASNA1 across species showing that it is highly conserved. (C) A point mutation in the FFAT-like motif of ASNA1 (F15A) abolishes its interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from U2OS cells using anti-Flag beads. (D) Indirect immunofluorescence of VAPB and Flag-ASNA1 WT. U2OS cells were transfected with Flag-ASNA1 WT for 24 hr. Flag-ASNA1 WT (red) is co-localized with VAPB (green) in a peri-nuclear area (enlarged window) suggesting an ER pattern. Scale bar is 10 μm. (E) ASNA1 interaction with FAF1 is strongly stimulated upon proteasome inhibition with MG132 and depends on the UBA domain. WT Flag-FAF1 and the indicated mutants were immunoprecipitated from U2OS cells. (F) G46R and G46A point mutations in ASNA1 abolish its interaction with BAG6 and strongly reduce its interaction with FAF1 and ubiquitin, most noticeably after MG132 treatment, but do not affect the interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from SH-SY5Y cells. DAPI, 4',6-diamidino-2-phenylindole; IP, immunoprecipitate; WT, wild type.
    Figure Legend Snippet: ASNA1 interacts with VAPB via a FFAT-like motif similar to FAF1. (A) Alignment of the FFAT-like motifs in human ASNA1 and FAF1. (B) Alignment of the FFAT-like motif of ASNA1 across species showing that it is highly conserved. (C) A point mutation in the FFAT-like motif of ASNA1 (F15A) abolishes its interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from U2OS cells using anti-Flag beads. (D) Indirect immunofluorescence of VAPB and Flag-ASNA1 WT. U2OS cells were transfected with Flag-ASNA1 WT for 24 hr. Flag-ASNA1 WT (red) is co-localized with VAPB (green) in a peri-nuclear area (enlarged window) suggesting an ER pattern. Scale bar is 10 μm. (E) ASNA1 interaction with FAF1 is strongly stimulated upon proteasome inhibition with MG132 and depends on the UBA domain. WT Flag-FAF1 and the indicated mutants were immunoprecipitated from U2OS cells. (F) G46R and G46A point mutations in ASNA1 abolish its interaction with BAG6 and strongly reduce its interaction with FAF1 and ubiquitin, most noticeably after MG132 treatment, but do not affect the interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from SH-SY5Y cells. DAPI, 4',6-diamidino-2-phenylindole; IP, immunoprecipitate; WT, wild type.

    Techniques Used: Mutagenesis, Immunoprecipitation, Immunofluorescence, Transfection, Inhibition

    bag6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bag6
    Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bag6/product/Cell Signaling Technology Inc
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    anti bag6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti bag6
    The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, <t>BAG6,</t> VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).
    Anti Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bag6/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bag6 - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Interaction profiling of RNA-binding ubiquitin ligases reveals a link between posttranscriptional regulation and the ubiquitin system"

    Article Title: Interaction profiling of RNA-binding ubiquitin ligases reveals a link between posttranscriptional regulation and the ubiquitin system

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16695-6

    The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, BAG6, VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).
    Figure Legend Snippet: The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, BAG6, VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).

    Techniques Used: Western Blot, Immunoprecipitation, Plasmid Preparation

    anti bag6 ab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti bag6 ab
    Anti Bag6 Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bag 6 8523 antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bag 6 8523 antibodies
    Bag 6 8523 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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  • 94
    Cell Signaling Technology Inc rabbit anti bag6
    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and <t>BAG6</t> binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.
    Rabbit Anti Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc bag6
    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and <t>BAG6</t> binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.
    Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti bag6
    The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, <t>BAG6,</t> VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).
    Anti Bag6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti bag6 ab
    The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, <t>BAG6,</t> VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).
    Anti Bag6 Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc bag 6 8523 antibodies
    The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, <t>BAG6,</t> VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).
    Bag 6 8523 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and BAG6 binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.

    Journal: BMC Biology

    Article Title: VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

    doi: 10.1186/1741-7007-12-39

    Figure Lengend Snippet: VAPB interaction with FAF1 and p97 is stimulated upon proteasome inhibition. (A) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to Flag-VAPB, while ASNA1 binding remains largely unchanged. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were grown in the presence of 100 ng/ml tetracycline for 24 hr or left untreated as a control. Flag-VAPB was immunoprecipitated using anti-Flag beads. (B) Proteasome inhibition enhances ubiquitin, p97 and FAF1 binding to endogenous VAPB, while ASNA1 binding was only slightly increased. Endogenous VAPB was immunoprecipitated from U2OS cells using anti-VAPB antibodies cross-linked to Protein A-beads. Uncoupled beads were used as control. (C, D) The binding of ubiquitinated proteins to VAPB is largely mediated by FAF1. U2OS cells were treated with the indicated siRNA oligos. Flag-VAPB (C) or endogenous VAPB (D) were immunoprecipitated as described above. Depletion of FAF1 strongly reduces the binding of ubiquitinated proteins to Flag- or endogenous VAPB. (E) The K87D M89D double mutant (KM-DD) of VAPB is defective in p97, FAF1, ASNA1 and ubiquitin binding. Flag-VAPB full-length WT, KM-DD and truncated, C-terminal half (C-Ter) and N-terminal half (MSP), were immunoprecipitated from U2OS cells. The MSP domain of VAPB is sufficient to interact with p97, FAF1 and ASNA1. KM-DD as well as the truncation lacking the MSP domain (C-Ter) are defective in binding poly-ubiquitinated proteins and seem to interact preferentially with oligo-ubiquitinated proteins. (F) The binding of ubiquitinated proteins to VAPB is reduced in cells treated with ASNA1 siRNA. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were treated with the indicated siRNA oligos. Depletion of ASNA1 reduces ubiquitin and BAG6 binding, but not FAF1 binding, to Flag-VAPB. C-Ter, C-terminal half; IP, immunoprecipitate; KM-DD, K87D M89D double mutant; Luc, luciferase.

    Article Snippet: The following antibodies were used: mouse anti-Flag M2 (Sigma, A8592), mouse anti-ubiquitin FK2 (Enzo, PW8810), rabbit anti-ubiquitin (Dako, Ely, UK; Z0458), mouse anti-p97 (Fitzgerald, North Acton, USA; 10R-P104A), rabbit anti-VAPA (Epitomics, Burlingame, USA; S1706), mouse anti-FAF1 (Abnova, Taipei City, Taiwan; H00011124-A01), rabbit anti-FAF1 (courtesy of Millipore, Billerica, USA), rabbit anti-GAPDH (Cell Signaling, 2118), rabbit anti-RAB3GAP1 (Proteintech, Manchester, UK; 21663-1-AP), rabbit anti-RAB3GAP2 (Abgent, Maidenhead, UK; AP9635B), rabbit anti-WDR44 (Bethyl, Montgomery, USA; A301-441A), mouse anti-ASNA1 (Abnova, H00000439-M03), rabbit anti-Syntaxin 1A (GeneTex, Irvine, USA; GTX113559), rabbit anti-BAG6 (Cell Signaling, 8523S), mouse anti-RPN2 (Abnova, H00006185-B01), rabbit anti-CD147 (Abcam, Cambridge, UK; ab108317), Alexa Fluor® 488 goat anti-rabbit (Invitrogen, A11008) and Alexa Fluor® 594 chicken anti-mouse (Invitrogen, A21201).

    Techniques: Inhibition, Binding Assay, Expressing, Immunoprecipitation, Mutagenesis, Luciferase

    TRC subunits were identified by mass spectrometry in Flag-FAF1 immunoprecipitates

    Journal: BMC Biology

    Article Title: VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

    doi: 10.1186/1741-7007-12-39

    Figure Lengend Snippet: TRC subunits were identified by mass spectrometry in Flag-FAF1 immunoprecipitates

    Article Snippet: The following antibodies were used: mouse anti-Flag M2 (Sigma, A8592), mouse anti-ubiquitin FK2 (Enzo, PW8810), rabbit anti-ubiquitin (Dako, Ely, UK; Z0458), mouse anti-p97 (Fitzgerald, North Acton, USA; 10R-P104A), rabbit anti-VAPA (Epitomics, Burlingame, USA; S1706), mouse anti-FAF1 (Abnova, Taipei City, Taiwan; H00011124-A01), rabbit anti-FAF1 (courtesy of Millipore, Billerica, USA), rabbit anti-GAPDH (Cell Signaling, 2118), rabbit anti-RAB3GAP1 (Proteintech, Manchester, UK; 21663-1-AP), rabbit anti-RAB3GAP2 (Abgent, Maidenhead, UK; AP9635B), rabbit anti-WDR44 (Bethyl, Montgomery, USA; A301-441A), mouse anti-ASNA1 (Abnova, H00000439-M03), rabbit anti-Syntaxin 1A (GeneTex, Irvine, USA; GTX113559), rabbit anti-BAG6 (Cell Signaling, 8523S), mouse anti-RPN2 (Abnova, H00006185-B01), rabbit anti-CD147 (Abcam, Cambridge, UK; ab108317), Alexa Fluor® 488 goat anti-rabbit (Invitrogen, A11008) and Alexa Fluor® 594 chicken anti-mouse (Invitrogen, A21201).

    Techniques: Mass Spectrometry

    TRC complex subunits slightly accumulate in endogenous VAPB immunoprecipitates upon proteasome inhibition

    Journal: BMC Biology

    Article Title: VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

    doi: 10.1186/1741-7007-12-39

    Figure Lengend Snippet: TRC complex subunits slightly accumulate in endogenous VAPB immunoprecipitates upon proteasome inhibition

    Article Snippet: The following antibodies were used: mouse anti-Flag M2 (Sigma, A8592), mouse anti-ubiquitin FK2 (Enzo, PW8810), rabbit anti-ubiquitin (Dako, Ely, UK; Z0458), mouse anti-p97 (Fitzgerald, North Acton, USA; 10R-P104A), rabbit anti-VAPA (Epitomics, Burlingame, USA; S1706), mouse anti-FAF1 (Abnova, Taipei City, Taiwan; H00011124-A01), rabbit anti-FAF1 (courtesy of Millipore, Billerica, USA), rabbit anti-GAPDH (Cell Signaling, 2118), rabbit anti-RAB3GAP1 (Proteintech, Manchester, UK; 21663-1-AP), rabbit anti-RAB3GAP2 (Abgent, Maidenhead, UK; AP9635B), rabbit anti-WDR44 (Bethyl, Montgomery, USA; A301-441A), mouse anti-ASNA1 (Abnova, H00000439-M03), rabbit anti-Syntaxin 1A (GeneTex, Irvine, USA; GTX113559), rabbit anti-BAG6 (Cell Signaling, 8523S), mouse anti-RPN2 (Abnova, H00006185-B01), rabbit anti-CD147 (Abcam, Cambridge, UK; ab108317), Alexa Fluor® 488 goat anti-rabbit (Invitrogen, A11008) and Alexa Fluor® 594 chicken anti-mouse (Invitrogen, A21201).

    Techniques:

    ASNA1 interacts with VAPB via a FFAT-like motif similar to FAF1. (A) Alignment of the FFAT-like motifs in human ASNA1 and FAF1. (B) Alignment of the FFAT-like motif of ASNA1 across species showing that it is highly conserved. (C) A point mutation in the FFAT-like motif of ASNA1 (F15A) abolishes its interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from U2OS cells using anti-Flag beads. (D) Indirect immunofluorescence of VAPB and Flag-ASNA1 WT. U2OS cells were transfected with Flag-ASNA1 WT for 24 hr. Flag-ASNA1 WT (red) is co-localized with VAPB (green) in a peri-nuclear area (enlarged window) suggesting an ER pattern. Scale bar is 10 μm. (E) ASNA1 interaction with FAF1 is strongly stimulated upon proteasome inhibition with MG132 and depends on the UBA domain. WT Flag-FAF1 and the indicated mutants were immunoprecipitated from U2OS cells. (F) G46R and G46A point mutations in ASNA1 abolish its interaction with BAG6 and strongly reduce its interaction with FAF1 and ubiquitin, most noticeably after MG132 treatment, but do not affect the interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from SH-SY5Y cells. DAPI, 4',6-diamidino-2-phenylindole; IP, immunoprecipitate; WT, wild type.

    Journal: BMC Biology

    Article Title: VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase

    doi: 10.1186/1741-7007-12-39

    Figure Lengend Snippet: ASNA1 interacts with VAPB via a FFAT-like motif similar to FAF1. (A) Alignment of the FFAT-like motifs in human ASNA1 and FAF1. (B) Alignment of the FFAT-like motif of ASNA1 across species showing that it is highly conserved. (C) A point mutation in the FFAT-like motif of ASNA1 (F15A) abolishes its interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from U2OS cells using anti-Flag beads. (D) Indirect immunofluorescence of VAPB and Flag-ASNA1 WT. U2OS cells were transfected with Flag-ASNA1 WT for 24 hr. Flag-ASNA1 WT (red) is co-localized with VAPB (green) in a peri-nuclear area (enlarged window) suggesting an ER pattern. Scale bar is 10 μm. (E) ASNA1 interaction with FAF1 is strongly stimulated upon proteasome inhibition with MG132 and depends on the UBA domain. WT Flag-FAF1 and the indicated mutants were immunoprecipitated from U2OS cells. (F) G46R and G46A point mutations in ASNA1 abolish its interaction with BAG6 and strongly reduce its interaction with FAF1 and ubiquitin, most noticeably after MG132 treatment, but do not affect the interaction with VAPB. WT and mutant Flag-ASNA1 were immunoprecipitated from SH-SY5Y cells. DAPI, 4',6-diamidino-2-phenylindole; IP, immunoprecipitate; WT, wild type.

    Article Snippet: The following antibodies were used: mouse anti-Flag M2 (Sigma, A8592), mouse anti-ubiquitin FK2 (Enzo, PW8810), rabbit anti-ubiquitin (Dako, Ely, UK; Z0458), mouse anti-p97 (Fitzgerald, North Acton, USA; 10R-P104A), rabbit anti-VAPA (Epitomics, Burlingame, USA; S1706), mouse anti-FAF1 (Abnova, Taipei City, Taiwan; H00011124-A01), rabbit anti-FAF1 (courtesy of Millipore, Billerica, USA), rabbit anti-GAPDH (Cell Signaling, 2118), rabbit anti-RAB3GAP1 (Proteintech, Manchester, UK; 21663-1-AP), rabbit anti-RAB3GAP2 (Abgent, Maidenhead, UK; AP9635B), rabbit anti-WDR44 (Bethyl, Montgomery, USA; A301-441A), mouse anti-ASNA1 (Abnova, H00000439-M03), rabbit anti-Syntaxin 1A (GeneTex, Irvine, USA; GTX113559), rabbit anti-BAG6 (Cell Signaling, 8523S), mouse anti-RPN2 (Abnova, H00006185-B01), rabbit anti-CD147 (Abcam, Cambridge, UK; ab108317), Alexa Fluor® 488 goat anti-rabbit (Invitrogen, A11008) and Alexa Fluor® 594 chicken anti-mouse (Invitrogen, A21201).

    Techniques: Mutagenesis, Immunoprecipitation, Immunofluorescence, Transfection, Inhibition

    The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, BAG6, VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).

    Journal: Scientific Reports

    Article Title: Interaction profiling of RNA-binding ubiquitin ligases reveals a link between posttranscriptional regulation and the ubiquitin system

    doi: 10.1038/s41598-017-16695-6

    Figure Lengend Snippet: The RBULs link posttranscriptional processes to the ubiquitin system. ( a ) The BP and MF GO terms of the interactors of the six RBULs were grouped into the categories “RNA” (blue), “Ubiquitin” (turquoise), and “Other” (rose). The distribution of the categories among the interactomes is shown. ( b ) A summary of GO terms for MEX3B interaction partners is shown. ( c , d ) Validation of RBUL interaction partners by pulldowns and Western blot. ( c ) Endogenous PRPF19 was pulled down with a PRPF19-specific antibody from HEK293T cells. Experiments omitting the antibody served as control. Western blot analysis was performed with antibodies specific against BAG2, BAG6, VCP, and HSPA1A, as well as against PRPF19 itself to validate the immunoprecipitation (IP). Left: Cropped images of input and IP samples (replicate 1). Images of full membranes and different exposure times for all antibodies and replicates are presented in Supplementary Figure . Right: Quantifications of the PRPF19-specific IPs normalized to control of three independent biological replicates are shown in a dot plot, including mean and standard error (s.e.m.). ( d ) GFP (empty vector, EV) and GFP-MEX3B were expressed in HEK293T cells and pulled down with a GFP-specific antibody. Western blot analysis was performed using specific antibodies against POLR1A, POLR3A, VCP, and HSPA1A, as well as GFP. Left: Cropped images of input and AP samples (replicate 1). Images of full membranes and different exposure times for all antibodies are presented in Supplementary Figure . Right: Quantification of the APs normalized to EV are shown as in ( c ).

    Article Snippet: The following antibodies were used: anti-GFP (B-2 clone; Santa Cruz; sc-9996), anti-HSP70 (Enzo Life Sciences; N15F2-5), anti-BAG6 (Cell Signaling Technology, 8523), anti-VCP (Cell Signaling Technology; 2649), anti-BAG2 (Sigma Alrich; HPA018862), anti-PRPF19 (Abcam; ab27692), anti-POLR1A (Santa Cruz; sc-48385), anti-POLR3A (D5Y2D; Cell Signaling Technology, 12825).

    Techniques: Western Blot, Immunoprecipitation, Plasmid Preparation