Journal: Annals of Translational Medicine

Article Title: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

doi: 10.21037/atm-21-5898

Figure Lengend Snippet: NGR2 increased hypoxia in the colon mucosa with an increased ratio of serum VEGFA165/VEGFA121 and increased protein expression of Rap1GAP and TSP1. (A) The protein expression levels of VEGFR2, VEGFR2 (p1175), VEGFR2 (p1214), Rap1GAP, TSP1, and HIF-1α in the colon mucosa; (B,C) the serum contents of VEGFA165 and VEGFA121 and the ratio of VEGFA165/VEGFA121; (D,E) the concentration of intracellular pyruvate and lactic acid in the colonic mucosa. The data are means ± SEM of 3 independent experiments performed in triplicate. One-way ANOVA and Student’s t-test (**, P<0.001 vs. control).

Article Snippet: Human VEGFA165 protein (#8065), LY294002 (PI3K inhibitor, #9901), anti-PI3K antibody (#4249), phospho-PI3K (#4228), Akt (#4691), phospho-Akt (#13038 and #4060) antibodies, goat anti-mouse antibody, and goat anti-rabbit antibody were from Cell Signaling Technology (USA).

Techniques: Expressing, Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

doi: 10.21037/atm-21-5898

Figure Lengend Snippet: NGR2 inhibited the cell proliferation and tube formation of pHUVECs induced by VEGFA165. (A,B) The cell viability and proliferation of pHUVECs; (C,D) the tube formation of pHUVECs (100×). The data are means ± SEM of 3 independent experiments performed in triplicate. One-way ANOVA and Student’s t-test (*P<0.05, **P<0.001 vs. control).

Article Snippet: Human VEGFA165 protein (#8065), LY294002 (PI3K inhibitor, #9901), anti-PI3K antibody (#4249), phospho-PI3K (#4228), Akt (#4691), phospho-Akt (#13038 and #4060) antibodies, goat anti-mouse antibody, and goat anti-rabbit antibody were from Cell Signaling Technology (USA).

Techniques:

Journal: Annals of Translational Medicine

Article Title: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

doi: 10.21037/atm-21-5898

Figure Lengend Snippet: Overexpression of Rap1GAP and NGR2 inhibited the cell proliferation of pHUVECs. (A) The gene expression of Rap1GAP in pHUVECs was up-regulated by Rap1GAPover plasmid; (B) the protein expression of Rap1GAP, TSP1, PI3K, phospho-PI3K, Akt, and phospho-Akt in pHUVECs treated with VEGFA165, NGR2, and Rap1GAPover plasmid; (C-F) the cell viability, cell proliferation, and concentration of intracellular pyruvate and lactic acid in pHUVECs treated with VEGFA165, NGR2, and Rap1GAPover plasmid; (G) the expression of Rap1GAP in pHUVECs was down-regulated by Rap1GAPsi plasmid; The data are means ± SEM of 3 independent experiments performed in triplicate. One-way ANOVA and Student’s t-test (*P<0.05, **P<0.001 vs. control).

Article Snippet: Human VEGFA165 protein (#8065), LY294002 (PI3K inhibitor, #9901), anti-PI3K antibody (#4249), phospho-PI3K (#4228), Akt (#4691), phospho-Akt (#13038 and #4060) antibodies, goat anti-mouse antibody, and goat anti-rabbit antibody were from Cell Signaling Technology (USA).

Techniques: Over Expression, Expressing, Plasmid Preparation, Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

doi: 10.21037/atm-21-5898

Figure Lengend Snippet: Silencing Rap1GAP promoted the cell proliferation of pHUVECs. (A) The protein expression of Rap1GAP, TSP1, PI3K, phospho-PI3K, Akt, and phospho-Akt in pHUVECs treated with VEGFA165, NGR2, and Rap1GAPsi plasmid; (B-E) the cell viability, cell proliferation, and concentration of intracellular pyruvate and lactic acid in pHUVECs treated with VEGFA165, NGR2, and Rap1GAPsi plasmid. The data are means ± SEM of 3 independent experiments performed in triplicate. One-way ANOVA and Student’s t-test (*P<0.05, **P<0.001 vs. control).

Article Snippet: Human VEGFA165 protein (#8065), LY294002 (PI3K inhibitor, #9901), anti-PI3K antibody (#4249), phospho-PI3K (#4228), Akt (#4691), phospho-Akt (#13038 and #4060) antibodies, goat anti-mouse antibody, and goat anti-rabbit antibody were from Cell Signaling Technology (USA).

Techniques: Expressing, Plasmid Preparation, Concentration Assay