thr286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc thr286
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 1 article reviews
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    1) Product Images from "Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats"

    Article Title: Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.101627

    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Figure Legend Snippet: TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.

    Techniques Used: Activity Assay, Western Blot

    Antibodies employed in this study.
    Figure Legend Snippet: Antibodies employed in this study.

    Techniques Used:

    thr286  (Cell Signaling Technology Inc)


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  • 88

    Structured Review

    Cell Signaling Technology Inc thr286
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88/100 stars

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    1) Product Images from "Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats"

    Article Title: Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.101627

    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Figure Legend Snippet: TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.

    Techniques Used: Activity Assay, Western Blot

    Antibodies employed in this study.
    Figure Legend Snippet: Antibodies employed in this study.

    Techniques Used:

    thr286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc thr286
    Antibodies employed in this study
    Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer’s Disease-Like Pathology and Cognitive Impairments"

    Article Title: Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer’s Disease-Like Pathology and Cognitive Impairments

    Journal: Journal of Alzheimer's Disease

    doi: 10.3233/JAD-180091

    Antibodies employed in this study
    Figure Legend Snippet: Antibodies employed in this study

    Techniques Used: Methylation

    Treatment with MO modulated tau related phosphatase and kinases. A) Western blotting of total protein phosphatase 2A (PP2Ac), methylated PP2Ac at Leu309 (M-L309), total glucose synthase kinase 3β (GSK3β), phosphorylated GSK3β at Ser9 (P-S9), total cyclin dependent kinase 5 (CDK5), activators of CDK5, p35 and p25, the total Ca 2 + /calmodulin-dependent protein kinase (CaMKII) and the phosphorylated CaMKII at Thr286 (P-CaMKII) from hippocampal lysates. B-J) Quantitative analysis of the blots, total PP2A, GSK3β, CDK5, p35, p25, and CaMKII were normalized to β-actin and phosphorylated GSK3β and CaMKII and methylated PP2Ac were normalized to their total proteins. The data were expressed as mean±SD ( n = 6). # p < 0.05, # # p < 0.01, # # # p < 0.001, # # # # p < 0.0001 versus control; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus Hcy.
    Figure Legend Snippet: Treatment with MO modulated tau related phosphatase and kinases. A) Western blotting of total protein phosphatase 2A (PP2Ac), methylated PP2Ac at Leu309 (M-L309), total glucose synthase kinase 3β (GSK3β), phosphorylated GSK3β at Ser9 (P-S9), total cyclin dependent kinase 5 (CDK5), activators of CDK5, p35 and p25, the total Ca 2 + /calmodulin-dependent protein kinase (CaMKII) and the phosphorylated CaMKII at Thr286 (P-CaMKII) from hippocampal lysates. B-J) Quantitative analysis of the blots, total PP2A, GSK3β, CDK5, p35, p25, and CaMKII were normalized to β-actin and phosphorylated GSK3β and CaMKII and methylated PP2Ac were normalized to their total proteins. The data were expressed as mean±SD ( n = 6). # p < 0.05, # # p < 0.01, # # # p < 0.001, # # # # p < 0.0001 versus control; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus Hcy.

    Techniques Used: Western Blot, Methylation

    p camk ii thr286 primary antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p camk ii thr286 primary antibodies
    P Camk Ii Thr286 Primary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thr 286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc thr 286
    Thr 286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thr 286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc thr 286
    Thr 286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thr286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc thr286
    Antibodies employed in the study
    Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Spatial training preserves associative memory capacity with augmentation of dendrite ramification and spine generation in Tg2576 mice"

    Article Title: Spatial training preserves associative memory capacity with augmentation of dendrite ramification and spine generation in Tg2576 mice

    Journal: Scientific Reports

    doi: 10.1038/srep09488

    Antibodies employed in the study
    Figure Legend Snippet: Antibodies employed in the study

    Techniques Used:

    anti phospho camkii thr286 287  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho camkii thr286 287
    Anti Phospho Camkii Thr286 287, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphorylated camkii thr286 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated camkii thr286 antibody
    Phosphorylated Camkii Thr286 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p camkiiα  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p camkiiα
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    thr286 camkii alpha ubi  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc thr286 camkii alpha ubi
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    Cell Signaling Technology Inc thr286
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thr286/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc p camk ii thr286 primary antibodies
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    P Camk Ii Thr286 Primary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p camk ii thr286 primary antibodies/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc thr 286
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Thr 286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thr 286/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti phospho camkii thr286 287
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Anti Phospho Camkii Thr286 287, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho camkii thr286 287/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc phosphorylated camkii thr286 antibody
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Phosphorylated Camkii Thr286 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    P Camkiiα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc thr286 camkii alpha ubi
    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at <t>Thr286</t> (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.
    Thr286 Camkii Alpha Ubi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.

    Journal: Aging (Albany NY)

    Article Title: Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats

    doi: 10.18632/aging.101627

    Figure Lengend Snippet: TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.

    Article Snippet: p-CaMKII , Phosphorylated CaMKII at Thr286 , 3361 , pAb , 1:1000 for WB , Cell Signaling Danvers, MA, USA.

    Techniques: Activity Assay, Western Blot

    Antibodies employed in this study.

    Journal: Aging (Albany NY)

    Article Title: Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats

    doi: 10.18632/aging.101627

    Figure Lengend Snippet: Antibodies employed in this study.

    Article Snippet: p-CaMKII , Phosphorylated CaMKII at Thr286 , 3361 , pAb , 1:1000 for WB , Cell Signaling Danvers, MA, USA.

    Techniques: