Journal: Aging (Albany NY)

Article Title: Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats

doi: 10.18632/aging.101627

Figure Lengend Snippet: TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca 2+ -dependent kinases CDK5 and CaMKII. ( A ) Western blots of total Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. ( B-F ) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. ( G ) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.

Article Snippet: p-CaMKII , Phosphorylated CaMKII at Thr286 , 3361 , pAb , 1:1000 for WB , Cell Signaling Danvers, MA, USA.

Techniques: Activity Assay, Western Blot

Journal: Aging (Albany NY)

Article Title: Methanolic extract of Tamarix Gallica attenuates hyperhomocysteinemia induced AD-like pathology and cognitive impairments in rats

doi: 10.18632/aging.101627

Figure Lengend Snippet: Antibodies employed in this study.

Article Snippet: p-CaMKII , Phosphorylated CaMKII at Thr286 , 3361 , pAb , 1:1000 for WB , Cell Signaling Danvers, MA, USA.

Techniques: