Antibody Against Jarid1d Kdm5d, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Human Sex Matters: Y-Linked Lysine Demethylase 5D Drives Accelerated Male Craniofacial Osteogenic Differentiation"
Article Title: Human Sex Matters: Y-Linked Lysine Demethylase 5D Drives Accelerated Male Craniofacial Osteogenic Differentiation
Figure Legend Snippet: Hierarchical clustering of differentially expressed genes revealed a sexually dimorphic osteogenic regulator profile between male and female NCSCs undergoing differentiation. ( A,B ) Cluster of upregulated genes in male NCSCs comprising autosomal osteogenic regulator CYP7B1 as well as the Y-linked genes KDM5D and USP9Y. ( C,D ). Female NCSCs showed a cluster of upregulated genes including the pro-osteogenic regulators KDM6A and XIST as well as the negative regulator TXLNG.
Figure Legend Snippet: ( A,B ) Ribosomal Protein S4 Y-Linked 1 (RPS4Y1) and KDM5D (depicted in cyan, arrows) were most significantly downregulated in female NCSCs after 14 days and 30 of differentiation compared their male counterparts. ( C ) Differentially expressed genes between male and female NCSCs belonged, among others, to the osteogenic-related KEGG pathways and GO terms (depicted in orange). * padj < 0.05 was considered significant.
Figure Legend Snippet: Knockdown of KDM5 significantly impaired the osteogenic differentiation capacity of male NCSCs. ( A ) Schematic view of the siRNA design. ( B ) qPCR analysis validating the knockdown of KDM5D. ( C ) Validation of the knockdown of KDM5D at the protein level via Western Blot. ( C – E ) Knockdown of KDM5D strongly inhibited the capability of male NCSCs to deposit calcium after 30 days of culture on collagen type I fibers compared to untreated and GFP-transduced cells. ( D–F ) Quantification of Alizarin Red S confirmed the significant inhibition of osteogenic differentiation in male NCSCs after knockdown of KDM5D. Mann–Whitney test, * p < 0.05 was considered significant.
Techniques Used: Western Blot, Inhibition, MANN-WHITNEY
Figure Legend Snippet: Pharmacological inhibition of KDM5D significantly reduced the capability of male NCSCs to undergo osteogenic differentiation. ( A,B ) Exposure of male NCSCs to increasing concentrations (1 µM–100 µM) of KDOAM-25 resulted in a significant reduction in calcium deposition after 30 days of differentiation compared to untreated control. ( C ) Quantification of Alizarin Red S confirmed the significant inhibition of male osteogenic differentiation upon inhibition of KDM5D by KDOAM-25-treatment. Mann–Whitney test, * p < 0.05 was considered significant. n.d.: not detectable.
Techniques Used: Inhibition, MANN-WHITNEY