Fitc Conjugated Tubulin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Inhibition of Mast Cell Activation of Radix Paeoniae alba Extraction Identified by TCRP Based and Conventional Cell Function Assay Systems"
Article Title: The Inhibition of Mast Cell Activation of Radix Paeoniae alba Extraction Identified by TCRP Based and Conventional Cell Function Assay Systems
Journal: PLoS ONE
Figure Legend Snippet: (A-B) IgE-mediated TCRP comparison with cell-electrode impedance dynamics induced by adenosine or PMA. RBL-2H3 cells were seeded on the E-plates overnight at a density of 20,000 cells/well, and were then treated with DNP-BSA (IgE-sensitized) and adenosine or PMA (no sensitization). (C) Representative distribution of CD63 stimulated by different antigens in RBL-2H3. RBL-2H3 cells with or without IgE sensitization were stimulated with DNP-BSA, adenosine, and PMA for 30 min. Bar, 5 μm. (D) Adenosine induced microtubule formation whereas PMA induced F-actin disassembly. Treated cells were fixed and processed for staining with phalloidin-rhodamine (red) and antibody to β-tubulin (green). Bar, 5 μm. (E) Adenosine together with PMA induced β-hexosaminidase release. The cells were seeded in 24-well plates overnight, pretreated with PMA or adenosine and subsequently activated with different concentrations of adenosine or PMA. *** P < 0.001 vs. with PMA or adenosine only.
Techniques Used: Staining