vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf
    BPC-157 promoted the expression <t>of</t> <t>VEGF-a</t> in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.
    Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Body protective compound-157 enhances alkali-burn wound healing in vivo and promotes proliferation, migration, and angiogenesis in vitro"

    Article Title: Body protective compound-157 enhances alkali-burn wound healing in vivo and promotes proliferation, migration, and angiogenesis in vitro

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/DDDT.S82030

    BPC-157 promoted the expression of VEGF-a in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: BPC-157 promoted the expression of VEGF-a in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Standard Deviation

    vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf
    BPC-157 promoted the expression <t>of</t> <t>VEGF-a</t> in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.
    Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Body protective compound-157 enhances alkali-burn wound healing in vivo and promotes proliferation, migration, and angiogenesis in vitro"

    Article Title: Body protective compound-157 enhances alkali-burn wound healing in vivo and promotes proliferation, migration, and angiogenesis in vitro

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/DDDT.S82030

    BPC-157 promoted the expression of VEGF-a in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: BPC-157 promoted the expression of VEGF-a in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Standard Deviation

    vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf
    αPD-L1 inhibits the migration and angiogenesis of TNBC promoted by TAM/M2. (A) Schematic diagram of the RAW264.7 cell CM preparation. (B and C) MDA-MB-231 and Hs578T cell proliferation and apoptosis were examined using MTT assay and flow cytometry when the cells were treated with CM. (D and E) MDA-MB-231 and Hs578T cell migration was assessed using wound healing and Transwell assays when the cells were treated with CM (magnification, ×200). (F) HUVEC microtubule formation capacity was assessed using the endothelial tube formation assay when the cells were treated with CM. (G) <t>VEGF,</t> <t>MMP2</t> and MMP9 protein expression levels in MDA-MB-231 and Hs578T cells treated with CM were examined using western blot analysis. * P<0.05, ** P<0.01 and *** P<0.001. ns, not significant; αPD-L1, programmed death-ligand 1 inhibitor; TAM/M2, tumor-associated macrophages/M2-type; CM,conditional media; VEGF, vascular endothelial growth factor.
    Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "PD-L1 mediates triple-negative breast cancer evolution via the regulation of TAM/M2 polarization"

    Article Title: PD-L1 mediates triple-negative breast cancer evolution via the regulation of TAM/M2 polarization

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2022.5440

    αPD-L1 inhibits the migration and angiogenesis of TNBC promoted by TAM/M2. (A) Schematic diagram of the RAW264.7 cell CM preparation. (B and C) MDA-MB-231 and Hs578T cell proliferation and apoptosis were examined using MTT assay and flow cytometry when the cells were treated with CM. (D and E) MDA-MB-231 and Hs578T cell migration was assessed using wound healing and Transwell assays when the cells were treated with CM (magnification, ×200). (F) HUVEC microtubule formation capacity was assessed using the endothelial tube formation assay when the cells were treated with CM. (G) VEGF, MMP2 and MMP9 protein expression levels in MDA-MB-231 and Hs578T cells treated with CM were examined using western blot analysis. * P<0.05, ** P<0.01 and *** P<0.001. ns, not significant; αPD-L1, programmed death-ligand 1 inhibitor; TAM/M2, tumor-associated macrophages/M2-type; CM,conditional media; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: αPD-L1 inhibits the migration and angiogenesis of TNBC promoted by TAM/M2. (A) Schematic diagram of the RAW264.7 cell CM preparation. (B and C) MDA-MB-231 and Hs578T cell proliferation and apoptosis were examined using MTT assay and flow cytometry when the cells were treated with CM. (D and E) MDA-MB-231 and Hs578T cell migration was assessed using wound healing and Transwell assays when the cells were treated with CM (magnification, ×200). (F) HUVEC microtubule formation capacity was assessed using the endothelial tube formation assay when the cells were treated with CM. (G) VEGF, MMP2 and MMP9 protein expression levels in MDA-MB-231 and Hs578T cells treated with CM were examined using western blot analysis. * P<0.05, ** P<0.01 and *** P<0.001. ns, not significant; αPD-L1, programmed death-ligand 1 inhibitor; TAM/M2, tumor-associated macrophages/M2-type; CM,conditional media; VEGF, vascular endothelial growth factor.

    Techniques Used: Migration, MTT Assay, Flow Cytometry, Endothelial Tube Formation Assay, Expressing, Western Blot

    αPD-L1 reverses TNBC malignant evolution in the co-culture of TNBC cells and TAMs. (A and B) MDA-MB-231 and Hs578T cell migration capacity was assessed using wound healing and Transwell assays when the cells were treated with or without αPD-L1 in a co-culture system (magnification, ×200). (C) Protein expression levels of VEGF, MMP2 and MMP9 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (D) Protein expression levels of the EMT markers, E-cadherin, ZO-1, N-cadherin, vimentin, Slug and Twist, in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (E) Protein expression levels of the stemness markers, CD44, Oct4, Nanog, Bmi1 and Sox2 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system were determined using western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; TAM/M2, tumor-associated macrophages/M2-type; VEGF, vascular endothelial growth factor; EMT, epithelial-mesenchymal transition; ZO-1, zonula occludens-1.
    Figure Legend Snippet: αPD-L1 reverses TNBC malignant evolution in the co-culture of TNBC cells and TAMs. (A and B) MDA-MB-231 and Hs578T cell migration capacity was assessed using wound healing and Transwell assays when the cells were treated with or without αPD-L1 in a co-culture system (magnification, ×200). (C) Protein expression levels of VEGF, MMP2 and MMP9 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (D) Protein expression levels of the EMT markers, E-cadherin, ZO-1, N-cadherin, vimentin, Slug and Twist, in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (E) Protein expression levels of the stemness markers, CD44, Oct4, Nanog, Bmi1 and Sox2 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system were determined using western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; TAM/M2, tumor-associated macrophages/M2-type; VEGF, vascular endothelial growth factor; EMT, epithelial-mesenchymal transition; ZO-1, zonula occludens-1.

    Techniques Used: Co-Culture Assay, Migration, Expressing, Western Blot

    αPD-L1 inhibits lung metastasis in vivo . (A) Schematic diagram of the animal assays performed. (B and C) Tumor volume in the subcutaneous cell-transplanted mice was recorded over time throughout the animal assay. (D and H) The number of lung metastatic nodules was determined. (E) Lung metastases in mice that were subcutaneously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (F and G) Body weight of subcutaneous and intravenous cell-transplanted mice was recorded over time throughout the animal assay. (I) Lung metastases in mice that were intravenously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (J and K) CD206 protein expression levels in lung and tumor tissues were assessed using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). (L-O) Protein expression levels of E-cadherin, vimentin, VEGF and CD44 in tumors tissues were determined using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). * P<0.05. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: αPD-L1 inhibits lung metastasis in vivo . (A) Schematic diagram of the animal assays performed. (B and C) Tumor volume in the subcutaneous cell-transplanted mice was recorded over time throughout the animal assay. (D and H) The number of lung metastatic nodules was determined. (E) Lung metastases in mice that were subcutaneously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (F and G) Body weight of subcutaneous and intravenous cell-transplanted mice was recorded over time throughout the animal assay. (I) Lung metastases in mice that were intravenously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (J and K) CD206 protein expression levels in lung and tumor tissues were assessed using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). (L-O) Protein expression levels of E-cadherin, vimentin, VEGF and CD44 in tumors tissues were determined using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). * P<0.05. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; VEGF, vascular endothelial growth factor.

    Techniques Used: In Vivo, Injection, Expressing, Immunohistochemistry

    vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf
    αPD-L1 reverses TNBC malignant evolution in the co-culture of TNBC cells and TAMs. (A and B) MDA-MB-231 and Hs578T cell migration capacity was assessed using wound healing and Transwell assays when the cells were treated with or without αPD-L1 in a co-culture system (magnification, ×200). (C) Protein expression levels of <t>VEGF,</t> MMP2 and MMP9 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (D) Protein expression levels of the EMT markers, E-cadherin, ZO-1, N-cadherin, vimentin, Slug and Twist, in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (E) Protein expression levels of the stemness <t>markers,</t> <t>CD44,</t> Oct4, Nanog, Bmi1 and Sox2 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system were determined using western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; TAM/M2, tumor-associated macrophages/M2-type; VEGF, vascular endothelial growth factor; EMT, epithelial-mesenchymal transition; ZO-1, zonula occludens-1.
    Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "PD-L1 mediates triple-negative breast cancer evolution via the regulation of TAM/M2 polarization"

    Article Title: PD-L1 mediates triple-negative breast cancer evolution via the regulation of TAM/M2 polarization

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2022.5440

    αPD-L1 reverses TNBC malignant evolution in the co-culture of TNBC cells and TAMs. (A and B) MDA-MB-231 and Hs578T cell migration capacity was assessed using wound healing and Transwell assays when the cells were treated with or without αPD-L1 in a co-culture system (magnification, ×200). (C) Protein expression levels of VEGF, MMP2 and MMP9 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (D) Protein expression levels of the EMT markers, E-cadherin, ZO-1, N-cadherin, vimentin, Slug and Twist, in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (E) Protein expression levels of the stemness markers, CD44, Oct4, Nanog, Bmi1 and Sox2 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system were determined using western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; TAM/M2, tumor-associated macrophages/M2-type; VEGF, vascular endothelial growth factor; EMT, epithelial-mesenchymal transition; ZO-1, zonula occludens-1.
    Figure Legend Snippet: αPD-L1 reverses TNBC malignant evolution in the co-culture of TNBC cells and TAMs. (A and B) MDA-MB-231 and Hs578T cell migration capacity was assessed using wound healing and Transwell assays when the cells were treated with or without αPD-L1 in a co-culture system (magnification, ×200). (C) Protein expression levels of VEGF, MMP2 and MMP9 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (D) Protein expression levels of the EMT markers, E-cadherin, ZO-1, N-cadherin, vimentin, Slug and Twist, in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system, were determined using western blot analysis. (E) Protein expression levels of the stemness markers, CD44, Oct4, Nanog, Bmi1 and Sox2 in MDA-MB-231 and Hs578T cells, with or without αPD-L1 in a co-culture system were determined using western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; TAM/M2, tumor-associated macrophages/M2-type; VEGF, vascular endothelial growth factor; EMT, epithelial-mesenchymal transition; ZO-1, zonula occludens-1.

    Techniques Used: Co-Culture Assay, Migration, Expressing, Western Blot

    αPD-L1 inhibits lung metastasis in vivo . (A) Schematic diagram of the animal assays performed. (B and C) Tumor volume in the subcutaneous cell-transplanted mice was recorded over time throughout the animal assay. (D and H) The number of lung metastatic nodules was determined. (E) Lung metastases in mice that were subcutaneously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (F and G) Body weight of subcutaneous and intravenous cell-transplanted mice was recorded over time throughout the animal assay. (I) Lung metastases in mice that were intravenously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (J and K) CD206 protein expression levels in lung and tumor tissues were assessed using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). (L-O) Protein expression levels of E-cadherin, vimentin, VEGF and CD44 in tumors tissues were determined using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). * P<0.05. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: αPD-L1 inhibits lung metastasis in vivo . (A) Schematic diagram of the animal assays performed. (B and C) Tumor volume in the subcutaneous cell-transplanted mice was recorded over time throughout the animal assay. (D and H) The number of lung metastatic nodules was determined. (E) Lung metastases in mice that were subcutaneously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (F and G) Body weight of subcutaneous and intravenous cell-transplanted mice was recorded over time throughout the animal assay. (I) Lung metastases in mice that were intravenously injected with TNBC cells. Lung tissue images, H&E scan images and H&E magnification images (magnification, ×100) are shown, respectively. (J and K) CD206 protein expression levels in lung and tumor tissues were assessed using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). (L-O) Protein expression levels of E-cadherin, vimentin, VEGF and CD44 in tumors tissues were determined using immunohistochemistry (magnification: upper panels, ×100, lower panels, ×200). * P<0.05. αPD-L1, programmed death-ligand 1 inhibitor; TNBC, triple-negative breast cancer; VEGF, vascular endothelial growth factor.

    Techniques Used: In Vivo, Injection, Expressing, Immunohistochemistry

    vascular endothelial growth factor vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vascular endothelial growth factor vegf
    Vascular Endothelial Growth Factor Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf
    Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monoclonal vegf sc 7269 santa cruz  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal vegf sc 7269 santa cruz
    List of primary antibodies used in the study
    Monoclonal Vegf Sc 7269 Santa Cruz, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal vegf sc 7269 santa cruz/product/Cell Signaling Technology Inc
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    1) Product Images from "Clinical significance of PI3K/Akt/mTOR signaling in gastric carcinoma"

    Article Title: Clinical significance of PI3K/Akt/mTOR signaling in gastric carcinoma

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    List of primary antibodies used in the study
    Figure Legend Snippet: List of primary antibodies used in the study

    Techniques Used:

    monoclonal vegf sc 7269 santa cruz  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal vegf sc 7269 santa cruz
    List of primary antibodies used in the study
    Monoclonal Vegf Sc 7269 Santa Cruz, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal vegf sc 7269 santa cruz/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Clinical significance of PI3K/Akt/mTOR signaling in gastric carcinoma"

    Article Title: Clinical significance of PI3K/Akt/mTOR signaling in gastric carcinoma

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    List of primary antibodies used in the study
    Figure Legend Snippet: List of primary antibodies used in the study

    Techniques Used:

    vascular endothelial growth factor vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vascular endothelial growth factor vegf
    (A) Ki-67, TUNEL and CD31 staining in tumors of mice treated with vehicle, OPC31260, Tolvaptan or dDAVP in tissue sections of tumors from studies in and . (B) Quantification of Ki-67, (C) TUNEL stained cells and (D) CD31 stained vessels (n=4 tumors each). (E) Western blot for <t>VEGF</t> and (F) Quantification for VEGF bands by densitometry relative <t>to</t> <t>GAPDH.</t> (G) Western blot for VEGF in Caki-1 cells treated with dDAVP (1nM), Tolvaptan (50μM) and OPC31260 (25μM) *P<0.05, **P<0.01, ***P<0.001 vs Vehicle. All error bars represent SEM. P values by two-tailed unpaired student’s t-test with Welch’s correction and F test in Fig 5B, 5C, 5D, 5F.
    Vascular Endothelial Growth Factor Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Targeting the Vasopressin Type 2 Receptor for Renal Cell Carcinoma Therapy"

    Article Title: Targeting the Vasopressin Type 2 Receptor for Renal Cell Carcinoma Therapy

    Journal: Oncogene

    doi: 10.1038/s41388-019-1059-0

    (A) Ki-67, TUNEL and CD31 staining in tumors of mice treated with vehicle, OPC31260, Tolvaptan or dDAVP in tissue sections of tumors from studies in and . (B) Quantification of Ki-67, (C) TUNEL stained cells and (D) CD31 stained vessels (n=4 tumors each). (E) Western blot for VEGF and (F) Quantification for VEGF bands by densitometry relative to GAPDH. (G) Western blot for VEGF in Caki-1 cells treated with dDAVP (1nM), Tolvaptan (50μM) and OPC31260 (25μM) *P<0.05, **P<0.01, ***P<0.001 vs Vehicle. All error bars represent SEM. P values by two-tailed unpaired student’s t-test with Welch’s correction and F test in Fig 5B, 5C, 5D, 5F.
    Figure Legend Snippet: (A) Ki-67, TUNEL and CD31 staining in tumors of mice treated with vehicle, OPC31260, Tolvaptan or dDAVP in tissue sections of tumors from studies in and . (B) Quantification of Ki-67, (C) TUNEL stained cells and (D) CD31 stained vessels (n=4 tumors each). (E) Western blot for VEGF and (F) Quantification for VEGF bands by densitometry relative to GAPDH. (G) Western blot for VEGF in Caki-1 cells treated with dDAVP (1nM), Tolvaptan (50μM) and OPC31260 (25μM) *P<0.05, **P<0.01, ***P<0.001 vs Vehicle. All error bars represent SEM. P values by two-tailed unpaired student’s t-test with Welch’s correction and F test in Fig 5B, 5C, 5D, 5F.

    Techniques Used: TUNEL Assay, Staining, Western Blot, Two Tailed Test

    vegf  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf
    Cyr61-FAK-PI3K signaling boosts <t>VEGF</t> expression. The effect of Cyr61 on the expression level of VEGF in RF/6 A cell was performed by RT-PCR ( a ), western blot ( b ) and ELISA ( c ). ( d ) RF/6 A cells were treated with an anti-α <t>ν</t> <t>β</t> 3 , anti-α ν β 5 , or anti-α 5 β 1 blocking antibody for 1 h. Following incubation with the inhibitors, the cells were stimulated with Cyr61 (40 ng/mL), and the levels of total (top panel) and secreted (bottom panel) VEGF protein were measured through western blot analysis and ELISA, respectively. ( e ) The cells were pretreated with a FAK inhibitor (PF573228) for 1 h, then stimulated with Cyr61 (40 ng/mL). The total (left panel) and secreted (right panel) MCP-1 protein levels were measured through western blot and ELISA analyses, respectively. ( f ) RF/6 A cells were stimulated as indicated and an immunofluorescence assay was performed 24 h later to detect the activation and translocation of NF-κB p65. ( g ) The effect of Cyr61 on the expression level of MMPs in RF/6 A cell was performed by western blot. Values are the mean ± SEM of at least 3 independent experiments. *P < 0.05 and **P < 0.01 compared between the indicated groups.
    Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway"

    Article Title: Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-14015-6

    Cyr61-FAK-PI3K signaling boosts VEGF expression. The effect of Cyr61 on the expression level of VEGF in RF/6 A cell was performed by RT-PCR ( a ), western blot ( b ) and ELISA ( c ). ( d ) RF/6 A cells were treated with an anti-α ν β 3 , anti-α ν β 5 , or anti-α 5 β 1 blocking antibody for 1 h. Following incubation with the inhibitors, the cells were stimulated with Cyr61 (40 ng/mL), and the levels of total (top panel) and secreted (bottom panel) VEGF protein were measured through western blot analysis and ELISA, respectively. ( e ) The cells were pretreated with a FAK inhibitor (PF573228) for 1 h, then stimulated with Cyr61 (40 ng/mL). The total (left panel) and secreted (right panel) MCP-1 protein levels were measured through western blot and ELISA analyses, respectively. ( f ) RF/6 A cells were stimulated as indicated and an immunofluorescence assay was performed 24 h later to detect the activation and translocation of NF-κB p65. ( g ) The effect of Cyr61 on the expression level of MMPs in RF/6 A cell was performed by western blot. Values are the mean ± SEM of at least 3 independent experiments. *P < 0.05 and **P < 0.01 compared between the indicated groups.
    Figure Legend Snippet: Cyr61-FAK-PI3K signaling boosts VEGF expression. The effect of Cyr61 on the expression level of VEGF in RF/6 A cell was performed by RT-PCR ( a ), western blot ( b ) and ELISA ( c ). ( d ) RF/6 A cells were treated with an anti-α ν β 3 , anti-α ν β 5 , or anti-α 5 β 1 blocking antibody for 1 h. Following incubation with the inhibitors, the cells were stimulated with Cyr61 (40 ng/mL), and the levels of total (top panel) and secreted (bottom panel) VEGF protein were measured through western blot analysis and ELISA, respectively. ( e ) The cells were pretreated with a FAK inhibitor (PF573228) for 1 h, then stimulated with Cyr61 (40 ng/mL). The total (left panel) and secreted (right panel) MCP-1 protein levels were measured through western blot and ELISA analyses, respectively. ( f ) RF/6 A cells were stimulated as indicated and an immunofluorescence assay was performed 24 h later to detect the activation and translocation of NF-κB p65. ( g ) The effect of Cyr61 on the expression level of MMPs in RF/6 A cell was performed by western blot. Values are the mean ± SEM of at least 3 independent experiments. *P < 0.05 and **P < 0.01 compared between the indicated groups.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Blocking Assay, Incubation, Immunofluorescence, Activation Assay, Translocation Assay

    vegf a  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc vegf a
    Vegf A, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc vegf
    BPC-157 promoted the expression <t>of</t> <t>VEGF-a</t> in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.
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    Cell Signaling Technology Inc vascular endothelial growth factor vegf
    BPC-157 promoted the expression <t>of</t> <t>VEGF-a</t> in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.
    Vascular Endothelial Growth Factor Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc monoclonal vegf sc 7269 santa cruz
    List of primary antibodies used in the study
    Monoclonal Vegf Sc 7269 Santa Cruz, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc vegf a
    List of primary antibodies used in the study
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    BPC-157 promoted the expression of VEGF-a in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.

    Journal: Drug Design, Development and Therapy

    Article Title: Body protective compound-157 enhances alkali-burn wound healing in vivo and promotes proliferation, migration, and angiogenesis in vitro

    doi: 10.2147/DDDT.S82030

    Figure Lengend Snippet: BPC-157 promoted the expression of VEGF-a in wounded skin tissues. Notes: ( A ) Western blot analysis of the effect of BPC-157 on VEGF-a protein expression. ( B ) Real-time PCR was used to test the effect of BPC-157 on VEGF-a mRNA expression. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t -test. * P <0.05 significantly different from the control group. Abbreviations: bFGF, basic fibroblast growth factor; BPC-157, body protective compound 157; PCR, polymerase chain reaction; SD, standard deviation; VEGF, vascular endothelial growth factor.

    Article Snippet: After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4°C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Standard Deviation

    List of primary antibodies used in the study

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Clinical significance of PI3K/Akt/mTOR signaling in gastric carcinoma

    doi:

    Figure Lengend Snippet: List of primary antibodies used in the study

    Article Snippet: Source Dilution Monoclonal or polyclonal pAkt 2118-1 Epitomics 1:30 monoclonal pAkt (Ser473) 3787 Cell Signaling 1:40 monoclonal pmTOR (Ser2448) (49F9) 2976 Cell Signaling 1:100 monoclonal PTEN (c-term) 1539-1 Epitomics 1:80 monoclonal p4EBP1 9455 Cell Signaling 1:100 polyclonal pS6 ribosomal protein 2215 Cell Signaling 1:100 polyclonal Phospho-p70 S6 Kinase (Thr389) 9205 Cell Signaling 1:40 polyclonal eIF4E 9742 Cell Signaling 1:80 polyclonal HIF-1alpha (Ab655) 610958 BD Biosciences 1:30 monoclonal VEGF SC-7269 Santa Cruz 1:1000 monoclonal Open in a separate window List of primary antibodies used in the study

    Techniques: