aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc aco2
    Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    aco2 - by Bioz Stars, 2023-01
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    aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc aco2
    Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aco2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aco2 - by Bioz Stars, 2023-01
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    aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc aco2
    a Conditioned media from WT and ATG7-KO HeLa stably expressing mCherry-Parkin treated with A/O for 24 h were collected. Extracellular vesicles (EVs) were isolated by differential ultracentrifugation and immunoblotted for mitochondria and small EV markers. b Control or muscle specific ATG7-KO ( Atg7 f/f ;Ckmm-cre ) mice were subjected to 3 consecutive days of exhaustive exercise. Serum EVs or TA muscle tissue were then harvested. Representative immunoblots and quantification of the mitochondria markers <t>(ACO2</t> and SDHA) from serum EVs are shown. Mean of n = 4 mice per group ±SEM. c Nanoparticle tracking analysis of EVs from WT and ATG7-KO cells untreated or treated with A/O for 24 h. d Conditioned media from 24 h A/O treated ATG7-KO HeLa stably expressing mCherry-Parkin were filtered (0.22 µm cutoff) before isolation of EVs by differential ultracentrifugation and immunoblotting. e Proteinase K protection assay of EVs isolated by differential ultracentrifugation from 24 h A/O treated ATG7-KO HeLa stably expressing mCherry-Parkin. f EVs from 24 h A/O treated ATG7-KO cells were separated by a 5–40% bottom-up iodixanol density flotation gradient and subjected to immunoblotting. *Non-specific bands.
    Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aco2/product/Cell Signaling Technology Inc
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    1) Product Images from "A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery"

    Article Title: A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery

    Journal: Nature Communications

    doi: 10.1038/s41467-022-31213-7

    a Conditioned media from WT and ATG7-KO HeLa stably expressing mCherry-Parkin treated with A/O for 24 h were collected. Extracellular vesicles (EVs) were isolated by differential ultracentrifugation and immunoblotted for mitochondria and small EV markers. b Control or muscle specific ATG7-KO ( Atg7 f/f ;Ckmm-cre ) mice were subjected to 3 consecutive days of exhaustive exercise. Serum EVs or TA muscle tissue were then harvested. Representative immunoblots and quantification of the mitochondria markers (ACO2 and SDHA) from serum EVs are shown. Mean of n = 4 mice per group ±SEM. c Nanoparticle tracking analysis of EVs from WT and ATG7-KO cells untreated or treated with A/O for 24 h. d Conditioned media from 24 h A/O treated ATG7-KO HeLa stably expressing mCherry-Parkin were filtered (0.22 µm cutoff) before isolation of EVs by differential ultracentrifugation and immunoblotting. e Proteinase K protection assay of EVs isolated by differential ultracentrifugation from 24 h A/O treated ATG7-KO HeLa stably expressing mCherry-Parkin. f EVs from 24 h A/O treated ATG7-KO cells were separated by a 5–40% bottom-up iodixanol density flotation gradient and subjected to immunoblotting. *Non-specific bands.
    Figure Legend Snippet: a Conditioned media from WT and ATG7-KO HeLa stably expressing mCherry-Parkin treated with A/O for 24 h were collected. Extracellular vesicles (EVs) were isolated by differential ultracentrifugation and immunoblotted for mitochondria and small EV markers. b Control or muscle specific ATG7-KO ( Atg7 f/f ;Ckmm-cre ) mice were subjected to 3 consecutive days of exhaustive exercise. Serum EVs or TA muscle tissue were then harvested. Representative immunoblots and quantification of the mitochondria markers (ACO2 and SDHA) from serum EVs are shown. Mean of n = 4 mice per group ±SEM. c Nanoparticle tracking analysis of EVs from WT and ATG7-KO cells untreated or treated with A/O for 24 h. d Conditioned media from 24 h A/O treated ATG7-KO HeLa stably expressing mCherry-Parkin were filtered (0.22 µm cutoff) before isolation of EVs by differential ultracentrifugation and immunoblotting. e Proteinase K protection assay of EVs isolated by differential ultracentrifugation from 24 h A/O treated ATG7-KO HeLa stably expressing mCherry-Parkin. f EVs from 24 h A/O treated ATG7-KO cells were separated by a 5–40% bottom-up iodixanol density flotation gradient and subjected to immunoblotting. *Non-specific bands.

    Techniques Used: Stable Transfection, Expressing, Isolation, Western Blot

    aconitate hydratase  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc aconitate hydratase
    The protein changes of mitochondria of hippocampus after 28 days of tail suspension in rats.
    Aconitate Hydratase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    aconitate hydratase - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity"

    Article Title: The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0265108

    The protein changes of mitochondria of hippocampus after 28 days of tail suspension in rats.
    Figure Legend Snippet: The protein changes of mitochondria of hippocampus after 28 days of tail suspension in rats.

    Techniques Used: Binding Assay

    (A) Protein levels of ACO2, DLST and CS were determined by immunoblotting. (B) The relative expressions of ACO2, DLST and CS were represented by the intensity ratio between the protein and the loading control (VDAC) in each lane. n = 4, error bars indicated standard deviations. *p<0.05; **p<0.01 compared with each control group.
    Figure Legend Snippet: (A) Protein levels of ACO2, DLST and CS were determined by immunoblotting. (B) The relative expressions of ACO2, DLST and CS were represented by the intensity ratio between the protein and the loading control (VDAC) in each lane. n = 4, error bars indicated standard deviations. *p<0.05; **p<0.01 compared with each control group.

    Techniques Used: Western Blot

    rabbit polyclonal anti aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti aco2

    Rabbit Polyclonal Anti Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    94/100 stars

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    1) Product Images from "Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver"

    Article Title: Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver

    Journal: iScience

    doi: 10.1016/j.isci.2022.103996


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Protease Inhibitor, Electron Microscopy, Activity Assay, Staining, Software

    aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc aco2

    Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aco2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    aco2 - by Bioz Stars, 2023-01
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    1) Product Images from "Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver"

    Article Title: Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver

    Journal: iScience

    doi: 10.1016/j.isci.2022.103996


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Protease Inhibitor, Electron Microscopy, Activity Assay, Staining, Software

    anti aco2 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti aco2 antibody
    Suggested molecular signaling events contributing to rFlaA:Betv1-mediated activation of BMDMs. Stimulation of BMDM with aggregated rFlaA:Betv1 results in both TLR5-dependent and -independent uptake, leading to the activation of both MyD88− and MAPK-mediated signaling cascades. rFlaA:Betv1-induced pro-inflammatory IL-6 and TNF-α secretion were shown to be MyD88− and MAPK-dependent, likely promoting the induction of IFN-γ-producing Th1 cells in BMDM:TC co-cultures. Western blot analysis confirmed rFlaA:Betv1 to trigger STAT3 activation, increasing the expression of HIF-1a, which is likely to be responsible for the observed IL-1β secretion. Metabolically, we found a MyD88-dependent phosphorylation of the mTOR target protein p70 S6 kinase, which together with HIF-1a likely mediated the observed shift towards enhanced glycolysis with increased levels of the glucose transporter Glut-1 and PFKFB3, and the induction of the Warburg effect while being paralleled by a decreased expression of mitochondrial proteins such as <t>ACO2</t> and reduced oxygen consumption rates, suggesting a disrupted Krebs cycle in these cells. Finally, TLR5, MyD88, and MAPK activation were shown to be involved in the rFlaA:Betv1-induced production of the anti-inflammatory cytokine IL-10 that likely caused the observed suppression of rBet v 1-induced Th1 and Th2 responses in BMDM:TC co-cultures.
    Anti Aco2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94/100 stars

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    1) Product Images from "The Flagellin:Allergen Fusion Protein rFlaA:Betv1 Induces a MyD88− and MAPK-Dependent Activation of Glucose Metabolism in Macrophages"

    Article Title: The Flagellin:Allergen Fusion Protein rFlaA:Betv1 Induces a MyD88− and MAPK-Dependent Activation of Glucose Metabolism in Macrophages

    Journal: Cells

    doi: 10.3390/cells10102614

    Suggested molecular signaling events contributing to rFlaA:Betv1-mediated activation of BMDMs. Stimulation of BMDM with aggregated rFlaA:Betv1 results in both TLR5-dependent and -independent uptake, leading to the activation of both MyD88− and MAPK-mediated signaling cascades. rFlaA:Betv1-induced pro-inflammatory IL-6 and TNF-α secretion were shown to be MyD88− and MAPK-dependent, likely promoting the induction of IFN-γ-producing Th1 cells in BMDM:TC co-cultures. Western blot analysis confirmed rFlaA:Betv1 to trigger STAT3 activation, increasing the expression of HIF-1a, which is likely to be responsible for the observed IL-1β secretion. Metabolically, we found a MyD88-dependent phosphorylation of the mTOR target protein p70 S6 kinase, which together with HIF-1a likely mediated the observed shift towards enhanced glycolysis with increased levels of the glucose transporter Glut-1 and PFKFB3, and the induction of the Warburg effect while being paralleled by a decreased expression of mitochondrial proteins such as ACO2 and reduced oxygen consumption rates, suggesting a disrupted Krebs cycle in these cells. Finally, TLR5, MyD88, and MAPK activation were shown to be involved in the rFlaA:Betv1-induced production of the anti-inflammatory cytokine IL-10 that likely caused the observed suppression of rBet v 1-induced Th1 and Th2 responses in BMDM:TC co-cultures.
    Figure Legend Snippet: Suggested molecular signaling events contributing to rFlaA:Betv1-mediated activation of BMDMs. Stimulation of BMDM with aggregated rFlaA:Betv1 results in both TLR5-dependent and -independent uptake, leading to the activation of both MyD88− and MAPK-mediated signaling cascades. rFlaA:Betv1-induced pro-inflammatory IL-6 and TNF-α secretion were shown to be MyD88− and MAPK-dependent, likely promoting the induction of IFN-γ-producing Th1 cells in BMDM:TC co-cultures. Western blot analysis confirmed rFlaA:Betv1 to trigger STAT3 activation, increasing the expression of HIF-1a, which is likely to be responsible for the observed IL-1β secretion. Metabolically, we found a MyD88-dependent phosphorylation of the mTOR target protein p70 S6 kinase, which together with HIF-1a likely mediated the observed shift towards enhanced glycolysis with increased levels of the glucose transporter Glut-1 and PFKFB3, and the induction of the Warburg effect while being paralleled by a decreased expression of mitochondrial proteins such as ACO2 and reduced oxygen consumption rates, suggesting a disrupted Krebs cycle in these cells. Finally, TLR5, MyD88, and MAPK activation were shown to be involved in the rFlaA:Betv1-induced production of the anti-inflammatory cytokine IL-10 that likely caused the observed suppression of rBet v 1-induced Th1 and Th2 responses in BMDM:TC co-cultures.

    Techniques Used: Activation Assay, Western Blot, Expressing, Metabolic Labelling

    antibodies aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies aco2
    (A) CRISPR-Cas9 mediated stable targeting of <t>ACO2</t> gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.
    Antibodies Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies aco2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    antibodies aco2 - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone"

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    Journal: Cancer research

    doi: 10.1158/0008-5472.CAN-20-1708

    (A) CRISPR-Cas9 mediated stable targeting of ACO2 gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.
    Figure Legend Snippet: (A) CRISPR-Cas9 mediated stable targeting of ACO2 gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.

    Techniques Used: CRISPR, Western Blot, Clone Assay

    (A) C4-2-WT and C4-2-ACO2-KD cells (n=5, each group) were used to measure oxygen consumption rates (OCR) sequentially before and after the addition of oligomycin (2 μM), FCCP (1 μM), and antimycin A (1 μM) by XF24 Extracellular Flux Analyzer. Data are shown in mean ± S.D. Each time point is statistically significant. **** P<0.000001, two-way ANOVA with Sidak’s multiple comparisons test.
    Figure Legend Snippet: (A) C4-2-WT and C4-2-ACO2-KD cells (n=5, each group) were used to measure oxygen consumption rates (OCR) sequentially before and after the addition of oligomycin (2 μM), FCCP (1 μM), and antimycin A (1 μM) by XF24 Extracellular Flux Analyzer. Data are shown in mean ± S.D. Each time point is statistically significant. **** P<0.000001, two-way ANOVA with Sidak’s multiple comparisons test.

    Techniques Used:

    (A) V5-tagged-ACO2 adenovirus were transduced to C4-2 cells fractionated into cytosolic (Cyto), mitochondrial (Mito), and nuclear (Nuc) compartments followed by immunoblotting with cytosolic, mitochondrial, and nuclear proteins β-tubulin, Tom20 and lamin A/C respectively.
    Figure Legend Snippet: (A) V5-tagged-ACO2 adenovirus were transduced to C4-2 cells fractionated into cytosolic (Cyto), mitochondrial (Mito), and nuclear (Nuc) compartments followed by immunoblotting with cytosolic, mitochondrial, and nuclear proteins β-tubulin, Tom20 and lamin A/C respectively.

    Techniques Used: Western Blot

    (A) PC3 cells were transfected with myc-ACO2-WT or myc-ACO2-K258R for 48 hours (n=4, each group). Immunofluorescence staining was performed with anti-myc (green) while being counterstained with mitotracker (red) and DAPI (blue) followed by confocal microscopy. Scale bars, 10 μm.
    Figure Legend Snippet: (A) PC3 cells were transfected with myc-ACO2-WT or myc-ACO2-K258R for 48 hours (n=4, each group). Immunofluorescence staining was performed with anti-myc (green) while being counterstained with mitotracker (red) and DAPI (blue) followed by confocal microscopy. Scale bars, 10 μm.

    Techniques Used: Transfection, Immunofluorescence, Staining, Confocal Microscopy

    (A) HEK293T cells were transduced with WT-SIRT3-Flag or HA-SIRT3-H248Y plasmid and immunoblotted for ACO2, HA, Flag, Actin, and Ac-K in input depicting WT-SIRT3-Flag or HA-SIRT3-H248Y expression. Transfected cell lysates were used for endogenous ACO2 and IgG (control) immunoprecipitation, followed by immunoblotting for ACO2 and Ac-K.
    Figure Legend Snippet: (A) HEK293T cells were transduced with WT-SIRT3-Flag or HA-SIRT3-H248Y plasmid and immunoblotted for ACO2, HA, Flag, Actin, and Ac-K in input depicting WT-SIRT3-Flag or HA-SIRT3-H248Y expression. Transfected cell lysates were used for endogenous ACO2 and IgG (control) immunoprecipitation, followed by immunoblotting for ACO2 and Ac-K.

    Techniques Used: Transduction, Plasmid Preparation, Expressing, Transfection, Immunoprecipitation, Western Blot

    antibodies aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies aco2
    (A) CRISPR-Cas9 mediated stable targeting of <t>ACO2</t> gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.
    Antibodies Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies aco2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies aco2 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone"

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    Journal: Cancer research

    doi: 10.1158/0008-5472.CAN-20-1708

    (A) CRISPR-Cas9 mediated stable targeting of ACO2 gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.
    Figure Legend Snippet: (A) CRISPR-Cas9 mediated stable targeting of ACO2 gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.

    Techniques Used: CRISPR, Western Blot, Clone Assay

    (A) C4-2-WT and C4-2-ACO2-KD cells (n=5, each group) were used to measure oxygen consumption rates (OCR) sequentially before and after the addition of oligomycin (2 μM), FCCP (1 μM), and antimycin A (1 μM) by XF24 Extracellular Flux Analyzer. Data are shown in mean ± S.D. Each time point is statistically significant. **** P<0.000001, two-way ANOVA with Sidak’s multiple comparisons test.
    Figure Legend Snippet: (A) C4-2-WT and C4-2-ACO2-KD cells (n=5, each group) were used to measure oxygen consumption rates (OCR) sequentially before and after the addition of oligomycin (2 μM), FCCP (1 μM), and antimycin A (1 μM) by XF24 Extracellular Flux Analyzer. Data are shown in mean ± S.D. Each time point is statistically significant. **** P<0.000001, two-way ANOVA with Sidak’s multiple comparisons test.

    Techniques Used:

    (A) V5-tagged-ACO2 adenovirus were transduced to C4-2 cells fractionated into cytosolic (Cyto), mitochondrial (Mito), and nuclear (Nuc) compartments followed by immunoblotting with cytosolic, mitochondrial, and nuclear proteins β-tubulin, Tom20 and lamin A/C respectively.
    Figure Legend Snippet: (A) V5-tagged-ACO2 adenovirus were transduced to C4-2 cells fractionated into cytosolic (Cyto), mitochondrial (Mito), and nuclear (Nuc) compartments followed by immunoblotting with cytosolic, mitochondrial, and nuclear proteins β-tubulin, Tom20 and lamin A/C respectively.

    Techniques Used: Western Blot

    (A) PC3 cells were transfected with myc-ACO2-WT or myc-ACO2-K258R for 48 hours (n=4, each group). Immunofluorescence staining was performed with anti-myc (green) while being counterstained with mitotracker (red) and DAPI (blue) followed by confocal microscopy. Scale bars, 10 μm.
    Figure Legend Snippet: (A) PC3 cells were transfected with myc-ACO2-WT or myc-ACO2-K258R for 48 hours (n=4, each group). Immunofluorescence staining was performed with anti-myc (green) while being counterstained with mitotracker (red) and DAPI (blue) followed by confocal microscopy. Scale bars, 10 μm.

    Techniques Used: Transfection, Immunofluorescence, Staining, Confocal Microscopy

    (A) HEK293T cells were transduced with WT-SIRT3-Flag or HA-SIRT3-H248Y plasmid and immunoblotted for ACO2, HA, Flag, Actin, and Ac-K in input depicting WT-SIRT3-Flag or HA-SIRT3-H248Y expression. Transfected cell lysates were used for endogenous ACO2 and IgG (control) immunoprecipitation, followed by immunoblotting for ACO2 and Ac-K.
    Figure Legend Snippet: (A) HEK293T cells were transduced with WT-SIRT3-Flag or HA-SIRT3-H248Y plasmid and immunoblotted for ACO2, HA, Flag, Actin, and Ac-K in input depicting WT-SIRT3-Flag or HA-SIRT3-H248Y expression. Transfected cell lysates were used for endogenous ACO2 and IgG (control) immunoprecipitation, followed by immunoblotting for ACO2 and Ac-K.

    Techniques Used: Transduction, Plasmid Preparation, Expressing, Transfection, Immunoprecipitation, Western Blot

    aco2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc aco2
    Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aco2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aco2 - by Bioz Stars, 2023-01
    94/100 stars

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    6571s rrid ab 2797630  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 6571s rrid ab 2797630
    Key Resources Table
    6571s Rrid Ab 2797630, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6571s rrid ab 2797630/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6571s rrid ab 2797630 - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Maintaining iron homeostasis is the key role of lysosomal acidity for cell proliferation"

    Article Title: Maintaining iron homeostasis is the key role of lysosomal acidity for cell proliferation

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2020.01.003

    Key Resources Table
    Figure Legend Snippet: Key Resources Table

    Techniques Used: Cell Viability Assay, Bicinchoninic Acid Protein Assay, Sample Prep, Flow Cytometry, Recombinant, Magnetic Beads, Labeling, SYBR Green Assay, CRISPR, Software, Microscopy, Real-time Polymerase Chain Reaction, Imaging

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    Cell Signaling Technology Inc aco2
    Aco2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aco2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    Suggested molecular signaling events contributing to rFlaA:Betv1-mediated activation of BMDMs. Stimulation of BMDM with aggregated rFlaA:Betv1 results in both TLR5-dependent and -independent uptake, leading to the activation of both MyD88− and MAPK-mediated signaling cascades. rFlaA:Betv1-induced pro-inflammatory IL-6 and TNF-α secretion were shown to be MyD88− and MAPK-dependent, likely promoting the induction of IFN-γ-producing Th1 cells in BMDM:TC co-cultures. Western blot analysis confirmed rFlaA:Betv1 to trigger STAT3 activation, increasing the expression of HIF-1a, which is likely to be responsible for the observed IL-1β secretion. Metabolically, we found a MyD88-dependent phosphorylation of the mTOR target protein p70 S6 kinase, which together with HIF-1a likely mediated the observed shift towards enhanced glycolysis with increased levels of the glucose transporter Glut-1 and PFKFB3, and the induction of the Warburg effect while being paralleled by a decreased expression of mitochondrial proteins such as <t>ACO2</t> and reduced oxygen consumption rates, suggesting a disrupted Krebs cycle in these cells. Finally, TLR5, MyD88, and MAPK activation were shown to be involved in the rFlaA:Betv1-induced production of the anti-inflammatory cytokine IL-10 that likely caused the observed suppression of rBet v 1-induced Th1 and Th2 responses in BMDM:TC co-cultures.
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    (A) CRISPR-Cas9 mediated stable targeting of <t>ACO2</t> gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.
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    Image Search Results


    The protein changes of mitochondria of hippocampus after 28 days of tail suspension in rats.

    Journal: PLoS ONE

    Article Title: The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity

    doi: 10.1371/journal.pone.0265108

    Figure Lengend Snippet: The protein changes of mitochondria of hippocampus after 28 days of tail suspension in rats.

    Article Snippet: The primary antibodies used were aconitate hydratase (ACO2, 6571), dihydrolipoamide S-succinyltransferase (DLST, 11954), citrate synthase (CS, 14309), and VDAC (4661), and all of the antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA.

    Techniques: Binding Assay

    (A) Protein levels of ACO2, DLST and CS were determined by immunoblotting. (B) The relative expressions of ACO2, DLST and CS were represented by the intensity ratio between the protein and the loading control (VDAC) in each lane. n = 4, error bars indicated standard deviations. *p<0.05; **p<0.01 compared with each control group.

    Journal: PLoS ONE

    Article Title: The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity

    doi: 10.1371/journal.pone.0265108

    Figure Lengend Snippet: (A) Protein levels of ACO2, DLST and CS were determined by immunoblotting. (B) The relative expressions of ACO2, DLST and CS were represented by the intensity ratio between the protein and the loading control (VDAC) in each lane. n = 4, error bars indicated standard deviations. *p<0.05; **p<0.01 compared with each control group.

    Article Snippet: The primary antibodies used were aconitate hydratase (ACO2, 6571), dihydrolipoamide S-succinyltransferase (DLST, 11954), citrate synthase (CS, 14309), and VDAC (4661), and all of the antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA.

    Techniques: Western Blot

    Journal: iScience

    Article Title: Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver

    doi: 10.1016/j.isci.2022.103996

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-ACO2 , Cell Signaling Technology , Cat# 6571; RRID: AB_2890191.

    Techniques: Recombinant, Western Blot, Protease Inhibitor, Electron Microscopy, Activity Assay, Staining, Software

    Suggested molecular signaling events contributing to rFlaA:Betv1-mediated activation of BMDMs. Stimulation of BMDM with aggregated rFlaA:Betv1 results in both TLR5-dependent and -independent uptake, leading to the activation of both MyD88− and MAPK-mediated signaling cascades. rFlaA:Betv1-induced pro-inflammatory IL-6 and TNF-α secretion were shown to be MyD88− and MAPK-dependent, likely promoting the induction of IFN-γ-producing Th1 cells in BMDM:TC co-cultures. Western blot analysis confirmed rFlaA:Betv1 to trigger STAT3 activation, increasing the expression of HIF-1a, which is likely to be responsible for the observed IL-1β secretion. Metabolically, we found a MyD88-dependent phosphorylation of the mTOR target protein p70 S6 kinase, which together with HIF-1a likely mediated the observed shift towards enhanced glycolysis with increased levels of the glucose transporter Glut-1 and PFKFB3, and the induction of the Warburg effect while being paralleled by a decreased expression of mitochondrial proteins such as ACO2 and reduced oxygen consumption rates, suggesting a disrupted Krebs cycle in these cells. Finally, TLR5, MyD88, and MAPK activation were shown to be involved in the rFlaA:Betv1-induced production of the anti-inflammatory cytokine IL-10 that likely caused the observed suppression of rBet v 1-induced Th1 and Th2 responses in BMDM:TC co-cultures.

    Journal: Cells

    Article Title: The Flagellin:Allergen Fusion Protein rFlaA:Betv1 Induces a MyD88− and MAPK-Dependent Activation of Glucose Metabolism in Macrophages

    doi: 10.3390/cells10102614

    Figure Lengend Snippet: Suggested molecular signaling events contributing to rFlaA:Betv1-mediated activation of BMDMs. Stimulation of BMDM with aggregated rFlaA:Betv1 results in both TLR5-dependent and -independent uptake, leading to the activation of both MyD88− and MAPK-mediated signaling cascades. rFlaA:Betv1-induced pro-inflammatory IL-6 and TNF-α secretion were shown to be MyD88− and MAPK-dependent, likely promoting the induction of IFN-γ-producing Th1 cells in BMDM:TC co-cultures. Western blot analysis confirmed rFlaA:Betv1 to trigger STAT3 activation, increasing the expression of HIF-1a, which is likely to be responsible for the observed IL-1β secretion. Metabolically, we found a MyD88-dependent phosphorylation of the mTOR target protein p70 S6 kinase, which together with HIF-1a likely mediated the observed shift towards enhanced glycolysis with increased levels of the glucose transporter Glut-1 and PFKFB3, and the induction of the Warburg effect while being paralleled by a decreased expression of mitochondrial proteins such as ACO2 and reduced oxygen consumption rates, suggesting a disrupted Krebs cycle in these cells. Finally, TLR5, MyD88, and MAPK activation were shown to be involved in the rFlaA:Betv1-induced production of the anti-inflammatory cytokine IL-10 that likely caused the observed suppression of rBet v 1-induced Th1 and Th2 responses in BMDM:TC co-cultures.

    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the following primary antibodies from Cell Signaling Technologies overnight at 4 °C: phospho-MAPK Family Antibody Sampler Kit (#9910), NF-κB Pathway Sampler Kit (#9936), mTOR Substrates Antibody Sampler Kit (#9862), Glycolysis Antibody Sampler Kit (#8337), anti-Phospho-Stat3 (Ser727) antibody (#9134), anti-HIF-1a antibody (#14179), anti-ACO2 antibody (#6571), and loading control anti-histone H3 antibody (#12648, HRP Conjugate) or anti-GAPDH antibody (#8884, HRP Conjugate).

    Techniques: Activation Assay, Western Blot, Expressing, Metabolic Labelling

    (A) CRISPR-Cas9 mediated stable targeting of ACO2 gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.

    Journal: Cancer research

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    doi: 10.1158/0008-5472.CAN-20-1708

    Figure Lengend Snippet: (A) CRISPR-Cas9 mediated stable targeting of ACO2 gene in C4-2 cells using three independent sgRNAs were confirmed by immunoblotting Wild Type (WT) and Knockdown (ACO2-KD) pooled clones. Actin was used as a loading control.

    Article Snippet: Immunohistochemistry Tumors were fixed and stained with primary antibodies ACO2 (Cell Signaling, 6571), Ki67 (Dako, M72K0), Firefly luciferase Antibody (Thermo, MA1-16880), and SIRT3 (Cell Signaling, 2627) followed by washing and incubated with secondary antibody (Thermo).

    Techniques: CRISPR, Western Blot, Clone Assay

    (A) C4-2-WT and C4-2-ACO2-KD cells (n=5, each group) were used to measure oxygen consumption rates (OCR) sequentially before and after the addition of oligomycin (2 μM), FCCP (1 μM), and antimycin A (1 μM) by XF24 Extracellular Flux Analyzer. Data are shown in mean ± S.D. Each time point is statistically significant. **** P<0.000001, two-way ANOVA with Sidak’s multiple comparisons test.

    Journal: Cancer research

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    doi: 10.1158/0008-5472.CAN-20-1708

    Figure Lengend Snippet: (A) C4-2-WT and C4-2-ACO2-KD cells (n=5, each group) were used to measure oxygen consumption rates (OCR) sequentially before and after the addition of oligomycin (2 μM), FCCP (1 μM), and antimycin A (1 μM) by XF24 Extracellular Flux Analyzer. Data are shown in mean ± S.D. Each time point is statistically significant. **** P<0.000001, two-way ANOVA with Sidak’s multiple comparisons test.

    Article Snippet: Immunohistochemistry Tumors were fixed and stained with primary antibodies ACO2 (Cell Signaling, 6571), Ki67 (Dako, M72K0), Firefly luciferase Antibody (Thermo, MA1-16880), and SIRT3 (Cell Signaling, 2627) followed by washing and incubated with secondary antibody (Thermo).

    Techniques:

    (A) V5-tagged-ACO2 adenovirus were transduced to C4-2 cells fractionated into cytosolic (Cyto), mitochondrial (Mito), and nuclear (Nuc) compartments followed by immunoblotting with cytosolic, mitochondrial, and nuclear proteins β-tubulin, Tom20 and lamin A/C respectively.

    Journal: Cancer research

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    doi: 10.1158/0008-5472.CAN-20-1708

    Figure Lengend Snippet: (A) V5-tagged-ACO2 adenovirus were transduced to C4-2 cells fractionated into cytosolic (Cyto), mitochondrial (Mito), and nuclear (Nuc) compartments followed by immunoblotting with cytosolic, mitochondrial, and nuclear proteins β-tubulin, Tom20 and lamin A/C respectively.

    Article Snippet: Immunohistochemistry Tumors were fixed and stained with primary antibodies ACO2 (Cell Signaling, 6571), Ki67 (Dako, M72K0), Firefly luciferase Antibody (Thermo, MA1-16880), and SIRT3 (Cell Signaling, 2627) followed by washing and incubated with secondary antibody (Thermo).

    Techniques: Western Blot

    (A) PC3 cells were transfected with myc-ACO2-WT or myc-ACO2-K258R for 48 hours (n=4, each group). Immunofluorescence staining was performed with anti-myc (green) while being counterstained with mitotracker (red) and DAPI (blue) followed by confocal microscopy. Scale bars, 10 μm.

    Journal: Cancer research

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    doi: 10.1158/0008-5472.CAN-20-1708

    Figure Lengend Snippet: (A) PC3 cells were transfected with myc-ACO2-WT or myc-ACO2-K258R for 48 hours (n=4, each group). Immunofluorescence staining was performed with anti-myc (green) while being counterstained with mitotracker (red) and DAPI (blue) followed by confocal microscopy. Scale bars, 10 μm.

    Article Snippet: Immunohistochemistry Tumors were fixed and stained with primary antibodies ACO2 (Cell Signaling, 6571), Ki67 (Dako, M72K0), Firefly luciferase Antibody (Thermo, MA1-16880), and SIRT3 (Cell Signaling, 2627) followed by washing and incubated with secondary antibody (Thermo).

    Techniques: Transfection, Immunofluorescence, Staining, Confocal Microscopy

    (A) HEK293T cells were transduced with WT-SIRT3-Flag or HA-SIRT3-H248Y plasmid and immunoblotted for ACO2, HA, Flag, Actin, and Ac-K in input depicting WT-SIRT3-Flag or HA-SIRT3-H248Y expression. Transfected cell lysates were used for endogenous ACO2 and IgG (control) immunoprecipitation, followed by immunoblotting for ACO2 and Ac-K.

    Journal: Cancer research

    Article Title: Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone

    doi: 10.1158/0008-5472.CAN-20-1708

    Figure Lengend Snippet: (A) HEK293T cells were transduced with WT-SIRT3-Flag or HA-SIRT3-H248Y plasmid and immunoblotted for ACO2, HA, Flag, Actin, and Ac-K in input depicting WT-SIRT3-Flag or HA-SIRT3-H248Y expression. Transfected cell lysates were used for endogenous ACO2 and IgG (control) immunoprecipitation, followed by immunoblotting for ACO2 and Ac-K.

    Article Snippet: Immunohistochemistry Tumors were fixed and stained with primary antibodies ACO2 (Cell Signaling, 6571), Ki67 (Dako, M72K0), Firefly luciferase Antibody (Thermo, MA1-16880), and SIRT3 (Cell Signaling, 2627) followed by washing and incubated with secondary antibody (Thermo).

    Techniques: Transduction, Plasmid Preparation, Expressing, Transfection, Immunoprecipitation, Western Blot

    Key Resources Table

    Journal: Molecular cell

    Article Title: Maintaining iron homeostasis is the key role of lysosomal acidity for cell proliferation

    doi: 10.1016/j.molcel.2020.01.003

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: ACO2 , Cell Signaling , 6571S RRID:AB_2797630.

    Techniques: Cell Viability Assay, Bicinchoninic Acid Protein Assay, Sample Prep, Flow Cytometry, Recombinant, Magnetic Beads, Labeling, SYBR Green Assay, CRISPR, Software, Microscopy, Real-time Polymerase Chain Reaction, Imaging