Journal: International Journal of Nanomedicine

Article Title: An MRI-visible non-viral vector for targeted Bcl-2 siRNA delivery to neuroblastoma

doi: 10.2147/IJN.S32900

Figure Lengend Snippet: T 2 -weighted imaging of SK-N-SH cells. ( A ) T 2 -weighted imaging of SK-N-SH cells after 2-hour incubations with scAb GD2 -PEG-g-PEI-SPION, PEG-g-PEI-SPION, and scAb GD2 -PEG-g-PEI-SPION/GD2 at Fe concentrations of 5, 10, 20 and 40 μg/mL in DMEM. Untreated cells were used as the control group. Cells were scanned with a 1.5T MRI scanner. ( B ) shows the normalized MR intensity of different polymers at various Fe concentrations (n = 3). Abbreviations: PEG-g-PEI-SPION, polyethylene glycol-grafted polyethylenimine superparamagnetic iron oxide nanoparticle; DMEM, Dulbecco’s modified eagle medium; MRI, magnetic resonance imaging; MR, magnetic resonance. with either nontargeting or targeting/GD2 polyplexes. The results are consistent with results obtained through RT-PCR and indicate that targeting polyplexes have a stronger suppressive effect on the Bcl-2 gene.

Article Snippet: We used SignalSilence Bcl-2 siRNA (Cell Signaling Technology, Inc, Danvers, MA, USA) to specifically inhibit Bcl-2 expression and promote tumor apoptosis.

Techniques: Imaging, Modification, Magnetic Resonance Imaging, Reverse Transcription Polymerase Chain Reaction

Journal: International Journal of Nanomedicine

Article Title: An MRI-visible non-viral vector for targeted Bcl-2 siRNA delivery to neuroblastoma

doi: 10.2147/IJN.S32900

Figure Lengend Snippet: Efficiencies of PEG-g-PEI-SPION/siRNA, scAb GD2 -PEG-g-PEI-SPION/ siRNA, and scAb GD2 -PEG-g-PEI-SPION/siRNA with free GD2 antibody in suppressing Bcl-2 gene expression in SK-N-SH cells (N/P = 10). ( A ) Suppression of Bcl-2 mRNA levels as quantified by real-time RT-PCR (n = 3), When compared to scAb GD2 -PEG-g-PEI-SPION/siRNA (targeting), * P < 0.01 for control, nontargeting and targeting/GD2 polyplexes. ( B ) Suppression of the protein expression of the Bcl-2 gene was evaluated by Western blot analysis. Abbreviations: PEG-g-PEI-SPION, polyethylene glycol-grafted polyethylenimine superparamagnetic iron oxide nanoparticle; siRNA, small interfering ribonucleic acid; RT-PCR, reverse transcription polymerase chain reaction; P , probability level.

Article Snippet: We used SignalSilence Bcl-2 siRNA (Cell Signaling Technology, Inc, Danvers, MA, USA) to specifically inhibit Bcl-2 expression and promote tumor apoptosis.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: International Journal of Nanomedicine

Article Title: An MRI-visible non-viral vector for targeted Bcl-2 siRNA delivery to neuroblastoma

doi: 10.2147/IJN.S32900

Figure Lengend Snippet: Immunohistological characteristics of SK-N-SH tumors (sections were immunostained with Bcl-2 monoclonal antibodies). The brown stains indicated Bcl-2 protein. ( A and B ) PBS control, ( C and D ) nontargeting polyplex therapy, ( E and F ) targeting polyplex therapy. The black arrow mark apoptotic cell with condensation or nuclei fragmentation. Abbreviation: PBS, phosphate buffered saline.

Article Snippet: We used SignalSilence Bcl-2 siRNA (Cell Signaling Technology, Inc, Danvers, MA, USA) to specifically inhibit Bcl-2 expression and promote tumor apoptosis.

Techniques:

Journal:

Article Title: Unlike Esophageal Squamous Cells, Barrett's Epithelial Cells Resist Apoptosis by Activating the Nuclear Factor-?B Pathway

doi: 10.1158/0008-5472.CAN-08-3703

Figure Lengend Snippet: Representative Western blots demonstrating expression of NF-κB, Bcl-2, cIAP-1, XIAP, and survivin following irradiation with 50-400 J/m2 UV-B in BAR-T, NES-G2T, and NES-B3T cell lines. Note the expression of these survival proteins increases in BAR-T cells, but decreases in the NES cell lines in response to UV-B. Note that low-dose UV-B irradiation (50 J/m2) virtually abolishes the expression of NF-κB and XIAP in the NES-B3T cells, but not in the NES-G2T cells.

Article Snippet: After 24 hours, the cells were transfected using the SignalSilence® NF-κB p65 or Bcl-2 siRNA (Cell Signaling Technology), per the manufacturer's instructions.

Techniques: Western Blot, Expressing, Irradiation

Journal:

Article Title: Unlike Esophageal Squamous Cells, Barrett's Epithelial Cells Resist Apoptosis by Activating the Nuclear Factor-?B Pathway

doi: 10.1158/0008-5472.CAN-08-3703

Figure Lengend Snippet: (A) Western blots for Bcl-2 expression following siRNA infection. Note that Bcl-2 protein expression is inhibited following infection with Bcl-2 siRNA at baseline and after treatment with 400 J/m2. (B) Results of UV-B irradiation and Bcl-2 siRNA on apoptosis as determined by Annexin V staining; siRNA against lamin A/C served as a control. Note that inhibition of Bcl-2 causes a small, but statistically significant increase in apoptosis in response to low dose UV-B irradiation. The bar graphs depict the mean + SEM of at least 3 individual experiments. (&, p<0.01 compared to non-irradiated corresponding control; *, p<0.001 compared to non-irradiated corresponding controls)

Article Snippet: After 24 hours, the cells were transfected using the SignalSilence® NF-κB p65 or Bcl-2 siRNA (Cell Signaling Technology), per the manufacturer's instructions.

Techniques: Western Blot, Expressing, Infection, Irradiation, Staining, Inhibition