anti immunoglobulin g igg  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti immunoglobulin g igg
    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
    Anti Immunoglobulin G Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Ubiquitin-specific peptidase 28 enhances STAT3 signaling and promotes cell growth in non-small-cell lung cancer"

    Article Title: Ubiquitin-specific peptidase 28 enhances STAT3 signaling and promotes cell growth in non-small-cell lung cancer

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S194917

    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using anti-IgG or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
    Figure Legend Snippet: USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using anti-IgG or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.

    Techniques Used: Transfection, Co-Immunoprecipitation Assay, Western Blot, Plasmid Preparation, Immunoprecipitation

    anti immunoglobulin g igg  (Cell Signaling Technology Inc)


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  • 98

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    Cell Signaling Technology Inc anti immunoglobulin g igg
    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
    Anti Immunoglobulin G Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Ubiquitin-specific peptidase 28 enhances STAT3 signaling and promotes cell growth in non-small-cell lung cancer"

    Article Title: Ubiquitin-specific peptidase 28 enhances STAT3 signaling and promotes cell growth in non-small-cell lung cancer

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S194917

    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using anti-IgG or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
    Figure Legend Snippet: USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using anti-IgG or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.

    Techniques Used: Transfection, Co-Immunoprecipitation Assay, Western Blot, Plasmid Preparation, Immunoprecipitation

    goat anti mouse igg h l alexa fluor 647 conjugated  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc goat anti mouse igg h l alexa fluor 647 conjugated
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    goat anti mouse igg hrp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc goat anti mouse igg hrp
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    anti goat igg  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti goat igg
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    goat anti mouse igg hrp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc goat anti mouse igg hrp
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    goat anti mouse igg hrp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc goat anti mouse igg hrp
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    polyclonal goat igg  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal goat igg
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    anti phospho specific akt igg  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho specific akt igg
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    secondary anti igg hrp ab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc secondary anti igg hrp ab
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    anti goat igg  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti goat igg
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    Cell Signaling Technology Inc anti immunoglobulin g igg
    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using <t>anti-IgG</t> or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.
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    USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using anti-IgG or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.

    Journal: OncoTargets and therapy

    Article Title: Ubiquitin-specific peptidase 28 enhances STAT3 signaling and promotes cell growth in non-small-cell lung cancer

    doi: 10.2147/OTT.S194917

    Figure Lengend Snippet: USP28 interacts with STAT3 and decreases its polyubiquitination level. Notes: ( A , B ) Myc-USP28 or Flag-STAT3 plasmids were transfected into HEK293T for 36 hours, and then whole cell lysates were prepared for reciprocal Co-IP and immunoblotting against specific antibodies as indicated. ( C ) H1299 cells were lysed for Co-IP by using anti-STAT3 antibody, followed by immunoblotting against USP28 and STAT3. ( D ) HEK293T cells were transfected with Myc-USP28 or EV along with HA-Ub plasmids for 36 hours. Cell lysates were then prepared for IP using anti-IgG or anti-STAT3 antibody, followed by immunoblotting against STAT3 and Ub-K48 (using K48-linkage specific polyubiquitin antibody). In addition, whole cell lysates were also prepared for immunoblotting against STAT3, Myc and GAPDH. Abbreviations: EV, empty vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HA-Ub, HA-ubiquitin; IB, immunoblotting; IP, immunoprecipitation; n.s., non-sense; STAT3, signal transducer and activator of transcription 3; USP, ubiquitin-specific protease.

    Article Snippet: Anti-p-STAT3, anti-STAT3, anti-immunoglobulin G (IgG), and anti-K48-linkage specific polyubiquitin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Transfection, Co-Immunoprecipitation Assay, Western Blot, Plasmid Preparation, Immunoprecipitation