mouse anti gfp monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti gfp monoclonal antibody
    (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence <t>of</t> <t>PBS</t> (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with <t>GFP</t> tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.
    Mouse Anti Gfp Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti gfp monoclonal antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti gfp monoclonal antibody - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Retigeric Acid B Attenuates the Virulence of Candida albicans via Inhibiting Adenylyl Cyclase Activity Targeted by Enhanced Farnesol Production"

    Article Title: Retigeric Acid B Attenuates the Virulence of Candida albicans via Inhibiting Adenylyl Cyclase Activity Targeted by Enhanced Farnesol Production

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0041624

    (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence of PBS (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with GFP tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.
    Figure Legend Snippet: (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence of PBS (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with GFP tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.

    Techniques Used: Infection, Negative Control, Positive Control, Fluorescence

    mouse anti gfp monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti gfp monoclonal antibody
    (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence <t>of</t> <t>PBS</t> (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with <t>GFP</t> tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.
    Mouse Anti Gfp Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti gfp monoclonal antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti gfp monoclonal antibody - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Retigeric Acid B Attenuates the Virulence of Candida albicans via Inhibiting Adenylyl Cyclase Activity Targeted by Enhanced Farnesol Production"

    Article Title: Retigeric Acid B Attenuates the Virulence of Candida albicans via Inhibiting Adenylyl Cyclase Activity Targeted by Enhanced Farnesol Production

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0041624

    (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence of PBS (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with GFP tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.
    Figure Legend Snippet: (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence of PBS (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with GFP tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.

    Techniques Used: Infection, Negative Control, Positive Control, Fluorescence

    gfp 4b10 mouse mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gfp 4b10 mouse mab
    List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.
    Gfp 4b10 Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp 4b10 mouse mab/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp 4b10 mouse mab - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells"

    Article Title: Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells

    Journal: Science Advances

    doi: 10.1126/sciadv.abm7375

    List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.
    Figure Legend Snippet: List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.

    Techniques Used: Software, Nuclear Magnetic Resonance, Labeling, Produced, Purification, Recombinant, Plasmid Preparation, Transfection, Western Blot, Expressing, Stable Transfection, Binding Assay, Double Knockout, Microscopy, High Content Screening

    mouse monoclonal anti gfp antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal anti gfp antibodies
    Mouse Monoclonal Anti Gfp Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti gfp antibodies/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    mouse monoclonal anti gfp antibodies - by Bioz Stars, 2023-01
    94/100 stars

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    mouse anti gfp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti gfp
    A. Macrophages of stage 13 w 1118 and bfc ko embryos were stained with anti-Crq (magenta) and anti-Bfc <t>(green)</t> <t>antibodies.</t> Scale bar = 20 μm. B. Representative fluorescence pictures of the <t>Bfc-GFP</t> expression in S2 cells; B’-B”‘. Anti-Bfc (green) stained S2 cells (B’) ; macrophages (showed Mp for short in figure) isolated from w 1118 larvae (B”) and bfc ko larvae (B”‘) ; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm. C. Yeast two-hybrid assays to detect the interaction of Bfc (Bait) with Srp (Prey). Different concentrations of the labeled yeast transformants were grown on SD-Trp-Leu-His plates. D . Crude protein extracts from blank S2 cells, transiently transfected S2 cells expressing Bfc-Flag, and HA-Srp or HA-Srp alone were immunoprecipitated with anti-Flag magnetic beads. WB detection was performed using anti-Flag and anti-HA antibodies. E-E’. Anti-Srp (green) stained control S2 cells as well as bfc RNAi-treated S2 cells; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm.
    Mouse Anti Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti gfp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti gfp - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "bfc , a novel serpent co-factor for the expression of croquemort , regulates efferocytosis in Drosophila melanogaster"

    Article Title: bfc , a novel serpent co-factor for the expression of croquemort , regulates efferocytosis in Drosophila melanogaster

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1009947

    A. Macrophages of stage 13 w 1118 and bfc ko embryos were stained with anti-Crq (magenta) and anti-Bfc (green) antibodies. Scale bar = 20 μm. B. Representative fluorescence pictures of the Bfc-GFP expression in S2 cells; B’-B”‘. Anti-Bfc (green) stained S2 cells (B’) ; macrophages (showed Mp for short in figure) isolated from w 1118 larvae (B”) and bfc ko larvae (B”‘) ; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm. C. Yeast two-hybrid assays to detect the interaction of Bfc (Bait) with Srp (Prey). Different concentrations of the labeled yeast transformants were grown on SD-Trp-Leu-His plates. D . Crude protein extracts from blank S2 cells, transiently transfected S2 cells expressing Bfc-Flag, and HA-Srp or HA-Srp alone were immunoprecipitated with anti-Flag magnetic beads. WB detection was performed using anti-Flag and anti-HA antibodies. E-E’. Anti-Srp (green) stained control S2 cells as well as bfc RNAi-treated S2 cells; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm.
    Figure Legend Snippet: A. Macrophages of stage 13 w 1118 and bfc ko embryos were stained with anti-Crq (magenta) and anti-Bfc (green) antibodies. Scale bar = 20 μm. B. Representative fluorescence pictures of the Bfc-GFP expression in S2 cells; B’-B”‘. Anti-Bfc (green) stained S2 cells (B’) ; macrophages (showed Mp for short in figure) isolated from w 1118 larvae (B”) and bfc ko larvae (B”‘) ; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm. C. Yeast two-hybrid assays to detect the interaction of Bfc (Bait) with Srp (Prey). Different concentrations of the labeled yeast transformants were grown on SD-Trp-Leu-His plates. D . Crude protein extracts from blank S2 cells, transiently transfected S2 cells expressing Bfc-Flag, and HA-Srp or HA-Srp alone were immunoprecipitated with anti-Flag magnetic beads. WB detection was performed using anti-Flag and anti-HA antibodies. E-E’. Anti-Srp (green) stained control S2 cells as well as bfc RNAi-treated S2 cells; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm.

    Techniques Used: Staining, Fluorescence, Expressing, Isolation, Labeling, Transfection, Immunoprecipitation, Magnetic Beads

    gfp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gfp
    <t>SLIT2</t> is downregulated in breast cancer cells. (A) The expression of SLIT2 in normal tissue and tumor tissue of most cancers. (B) The expression of SLIT2 in normal tissue and tumor tissue of BRCA. (C) Kaplan–Meier survival curves for SLIT2 in BRCA. (D) IHC staining of SLIT2 in tumor or peri-tumor of BRCA patients. BRCA, breast cancer; IHC, immunohistochemistry. ** p < 0.01,*** p < 0.001, **** p < 0.0001.
    Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Enhancer RNA SLIT2 Inhibits Bone Metastasis of Breast Cancer Through Regulating P38 MAPK/c-Fos Signaling Pathway"

    Article Title: Enhancer RNA SLIT2 Inhibits Bone Metastasis of Breast Cancer Through Regulating P38 MAPK/c-Fos Signaling Pathway

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2021.743840

    SLIT2 is downregulated in breast cancer cells. (A) The expression of SLIT2 in normal tissue and tumor tissue of most cancers. (B) The expression of SLIT2 in normal tissue and tumor tissue of BRCA. (C) Kaplan–Meier survival curves for SLIT2 in BRCA. (D) IHC staining of SLIT2 in tumor or peri-tumor of BRCA patients. BRCA, breast cancer; IHC, immunohistochemistry. ** p < 0.01,*** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: SLIT2 is downregulated in breast cancer cells. (A) The expression of SLIT2 in normal tissue and tumor tissue of most cancers. (B) The expression of SLIT2 in normal tissue and tumor tissue of BRCA. (C) Kaplan–Meier survival curves for SLIT2 in BRCA. (D) IHC staining of SLIT2 in tumor or peri-tumor of BRCA patients. BRCA, breast cancer; IHC, immunohistochemistry. ** p < 0.01,*** p < 0.001, **** p < 0.0001.

    Techniques Used: Expressing, Immunohistochemistry

    SLIT2 inhibited the proliferation and migration of breast cancer cells. (A) The efficacy of SLIT2 knockdown or SLIT2 overexpression was examined by qRT-PCR and immunoblotting (B) . (C) Cell proliferation assay was performed by CCK8 in SLIT2 knockdown or SLIT2 overexpression cells. (D, E) Cell cycle assay in SLIT2 knockdown or SLIT2 overexpression cells. (F, G) Cell migration assay was performed by transwell in SLIT2 knockdown or SLIT2 overexpression cells. (H, I) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown or SLIT2 overexpression cells. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 and **** p < 0.0001. CCK8, Cell Counting Kit-8.
    Figure Legend Snippet: SLIT2 inhibited the proliferation and migration of breast cancer cells. (A) The efficacy of SLIT2 knockdown or SLIT2 overexpression was examined by qRT-PCR and immunoblotting (B) . (C) Cell proliferation assay was performed by CCK8 in SLIT2 knockdown or SLIT2 overexpression cells. (D, E) Cell cycle assay in SLIT2 knockdown or SLIT2 overexpression cells. (F, G) Cell migration assay was performed by transwell in SLIT2 knockdown or SLIT2 overexpression cells. (H, I) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown or SLIT2 overexpression cells. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 and **** p < 0.0001. CCK8, Cell Counting Kit-8.

    Techniques Used: Migration, Over Expression, Quantitative RT-PCR, Western Blot, Proliferation Assay, Cell Cycle Assay, Cell Migration Assay, Invasion Assay, Standard Deviation, Cell Counting

    SLIT2 regulates breast cancer proliferation and migration through MAPK/FOS signaling pathway. (A) Co-immunoprecipitation (IP) of SLIT2 interacts with MAPK in SLIT2-expressing MCF7 cells. (B) Western blotting of the phospho-MAPK/total-MAPK and phospho-c-Fos/total-c-Fos from three independent experiments in SLIT2 knockdown or SLIT2 overexpression cells with or without p38 MAPK inhibitor SB202190 (10 μM). (C) Cell cycle assay of in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (D) Cell migration assay was performed by transwell in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (E) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown with or without MAPK inhibitor SB202190. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). * p < 0.05, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: SLIT2 regulates breast cancer proliferation and migration through MAPK/FOS signaling pathway. (A) Co-immunoprecipitation (IP) of SLIT2 interacts with MAPK in SLIT2-expressing MCF7 cells. (B) Western blotting of the phospho-MAPK/total-MAPK and phospho-c-Fos/total-c-Fos from three independent experiments in SLIT2 knockdown or SLIT2 overexpression cells with or without p38 MAPK inhibitor SB202190 (10 μM). (C) Cell cycle assay of in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (D) Cell migration assay was performed by transwell in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (E) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown with or without MAPK inhibitor SB202190. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). * p < 0.05, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: Migration, Immunoprecipitation, Expressing, Western Blot, Over Expression, Cell Cycle Assay, Cell Migration Assay, Invasion Assay, Standard Deviation

    gfp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gfp
    Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp - by Bioz Stars, 2023-01
    94/100 stars

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    monoclonal mouse antibody against gfp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal mouse antibody against gfp
    Track of implanted cells labeled with <t>GFP</t> signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining <t>using</t> <t>monoclonal</t> antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.
    Monoclonal Mouse Antibody Against Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse antibody against gfp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse antibody against gfp - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Unfavorable Contribution of a Tissue-Engineering Cartilage Graft to Osteochondral Defect Repair in Young Rabbits"

    Article Title: Unfavorable Contribution of a Tissue-Engineering Cartilage Graft to Osteochondral Defect Repair in Young Rabbits

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2020.595518

    Track of implanted cells labeled with GFP signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining using monoclonal antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.
    Figure Legend Snippet: Track of implanted cells labeled with GFP signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining using monoclonal antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.

    Techniques Used: Labeling, Immunofluorescence, Expressing, In Vitro, Construct, Immunohistochemical staining, Staining, Negative Staining, In Vivo

    mouse anti gfp mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti gfp mab
    Mouse Anti Gfp Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    anti mouse gfp monoclonal antibodies  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti mouse gfp monoclonal antibodies
    Trehalose promoted the production of autophagosomes and suppressed MMT <t>in</t> <t>peritoneal</t> mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of <t>GFP</t> protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).
    Anti Mouse Gfp Monoclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells"

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-71230-4

    Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).
    Figure Legend Snippet: Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Techniques Used: Expressing, Inhibition, Incubation

    Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.
    Figure Legend Snippet: Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.

    Techniques Used: In Vivo, In Vitro, Incubation

    anti mouse gfp monoclonal antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti mouse gfp monoclonal antibody
    Trehalose promoted the production of autophagosomes and suppressed MMT <t>in</t> <t>peritoneal</t> mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of <t>GFP</t> protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).
    Anti Mouse Gfp Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94/100 stars

    Images

    1) Product Images from "Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells"

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-71230-4

    Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).
    Figure Legend Snippet: Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Techniques Used: Expressing, Inhibition, Incubation

    Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.
    Figure Legend Snippet: Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.

    Techniques Used: In Vivo, In Vitro, Incubation

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    Cell Signaling Technology Inc mouse anti gfp monoclonal antibody
    (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence <t>of</t> <t>PBS</t> (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with <t>GFP</t> tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.
    Mouse Anti Gfp Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc gfp 4b10 mouse mab
    List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.
    Gfp 4b10 Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse monoclonal anti gfp antibodies
    List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.
    Mouse Monoclonal Anti Gfp Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse anti gfp
    A. Macrophages of stage 13 w 1118 and bfc ko embryos were stained with anti-Crq (magenta) and anti-Bfc <t>(green)</t> <t>antibodies.</t> Scale bar = 20 μm. B. Representative fluorescence pictures of the <t>Bfc-GFP</t> expression in S2 cells; B’-B”‘. Anti-Bfc (green) stained S2 cells (B’) ; macrophages (showed Mp for short in figure) isolated from w 1118 larvae (B”) and bfc ko larvae (B”‘) ; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm. C. Yeast two-hybrid assays to detect the interaction of Bfc (Bait) with Srp (Prey). Different concentrations of the labeled yeast transformants were grown on SD-Trp-Leu-His plates. D . Crude protein extracts from blank S2 cells, transiently transfected S2 cells expressing Bfc-Flag, and HA-Srp or HA-Srp alone were immunoprecipitated with anti-Flag magnetic beads. WB detection was performed using anti-Flag and anti-HA antibodies. E-E’. Anti-Srp (green) stained control S2 cells as well as bfc RNAi-treated S2 cells; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm.
    Mouse Anti Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc gfp
    <t>SLIT2</t> is downregulated in breast cancer cells. (A) The expression of SLIT2 in normal tissue and tumor tissue of most cancers. (B) The expression of SLIT2 in normal tissue and tumor tissue of BRCA. (C) Kaplan–Meier survival curves for SLIT2 in BRCA. (D) IHC staining of SLIT2 in tumor or peri-tumor of BRCA patients. BRCA, breast cancer; IHC, immunohistochemistry. ** p < 0.01,*** p < 0.001, **** p < 0.0001.
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    Cell Signaling Technology Inc monoclonal mouse antibody against gfp
    Track of implanted cells labeled with <t>GFP</t> signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining <t>using</t> <t>monoclonal</t> antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.
    Monoclonal Mouse Antibody Against Gfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse anti gfp mab
    Track of implanted cells labeled with <t>GFP</t> signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining <t>using</t> <t>monoclonal</t> antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.
    Mouse Anti Gfp Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti mouse gfp monoclonal antibodies
    Trehalose promoted the production of autophagosomes and suppressed MMT <t>in</t> <t>peritoneal</t> mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of <t>GFP</t> protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).
    Anti Mouse Gfp Monoclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti mouse gfp monoclonal antibody
    Trehalose promoted the production of autophagosomes and suppressed MMT <t>in</t> <t>peritoneal</t> mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of <t>GFP</t> protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).
    Anti Mouse Gfp Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence of PBS (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with GFP tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.

    Journal: PLoS ONE

    Article Title: Retigeric Acid B Attenuates the Virulence of Candida albicans via Inhibiting Adenylyl Cyclase Activity Targeted by Enhanced Farnesol Production

    doi: 10.1371/journal.pone.0041624

    Figure Lengend Snippet: (A) Nematodes were infected with C. albicans YEM30 for 2 h and then moved to pathogen-free liquid media in the presence of PBS (negative control), RAB (4 or 16 µg/ml) or 2 µg/ml of FLC (positive control). Each day the worms were monitored and the survival rate was calculated. p <0.001 for 4RAB, 16RAB and FLC treated groups compared to PBS-treated group. (B) Nematodes were infected with the indicated C. albicans strains and then treated by a series of doses of RAB. After 5 days of therapy, the survival ratio in each group was calculated. (C) The nematodes were infected by C. albicans strain CASA1 with GFP tagged CDR1 for 2 h and then moved into pathogen-free liquid media containing drugs or the vehicle for 5 days. And then they were observed in 4× objective magnification. Most nematodes in control group did not demonstrate any movement and developed filaments outside the body. However, the majority of the drug-treated were alive and not invaded by the hyphae (scale bars: 200 µm). Insert shows the images of nematodes captured at 200× magnification (scale bars: 100 µm). The representative worm was selected for confocal microscopic observation. Green fluorescence of hyphae cells and yeast cells was seen within the intestine of the dead nematode under the vehicle treatment. And no C. albicans cells were observed in the live worm challenged by RAB (about 60 nematodes in each group). (D) Enlarged view of the rectangle frame.

    Article Snippet: Subsequently, the fixed cells were washed three times with PBS and incubated with mouse anti-GFP monoclonal antibody (Cell Signaling Technology, Beverly, MA) diluted 1∶200 in PBS with 1% BSA for 1 h at room temperature.

    Techniques: Infection, Negative Control, Positive Control, Fluorescence

    List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.

    Journal: Science Advances

    Article Title: Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells

    doi: 10.1126/sciadv.abm7375

    Figure Lengend Snippet: List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.

    Article Snippet: GFP (4B10) Mouse mAb , Cell Signaling Technologies , Catalog no. 2955S, RRID:AB_1196614.

    Techniques: Software, Nuclear Magnetic Resonance, Labeling, Produced, Purification, Recombinant, Plasmid Preparation, Transfection, Western Blot, Expressing, Stable Transfection, Binding Assay, Double Knockout, Microscopy, High Content Screening

    A. Macrophages of stage 13 w 1118 and bfc ko embryos were stained with anti-Crq (magenta) and anti-Bfc (green) antibodies. Scale bar = 20 μm. B. Representative fluorescence pictures of the Bfc-GFP expression in S2 cells; B’-B”‘. Anti-Bfc (green) stained S2 cells (B’) ; macrophages (showed Mp for short in figure) isolated from w 1118 larvae (B”) and bfc ko larvae (B”‘) ; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm. C. Yeast two-hybrid assays to detect the interaction of Bfc (Bait) with Srp (Prey). Different concentrations of the labeled yeast transformants were grown on SD-Trp-Leu-His plates. D . Crude protein extracts from blank S2 cells, transiently transfected S2 cells expressing Bfc-Flag, and HA-Srp or HA-Srp alone were immunoprecipitated with anti-Flag magnetic beads. WB detection was performed using anti-Flag and anti-HA antibodies. E-E’. Anti-Srp (green) stained control S2 cells as well as bfc RNAi-treated S2 cells; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm.

    Journal: PLoS Genetics

    Article Title: bfc , a novel serpent co-factor for the expression of croquemort , regulates efferocytosis in Drosophila melanogaster

    doi: 10.1371/journal.pgen.1009947

    Figure Lengend Snippet: A. Macrophages of stage 13 w 1118 and bfc ko embryos were stained with anti-Crq (magenta) and anti-Bfc (green) antibodies. Scale bar = 20 μm. B. Representative fluorescence pictures of the Bfc-GFP expression in S2 cells; B’-B”‘. Anti-Bfc (green) stained S2 cells (B’) ; macrophages (showed Mp for short in figure) isolated from w 1118 larvae (B”) and bfc ko larvae (B”‘) ; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm. C. Yeast two-hybrid assays to detect the interaction of Bfc (Bait) with Srp (Prey). Different concentrations of the labeled yeast transformants were grown on SD-Trp-Leu-His plates. D . Crude protein extracts from blank S2 cells, transiently transfected S2 cells expressing Bfc-Flag, and HA-Srp or HA-Srp alone were immunoprecipitated with anti-Flag magnetic beads. WB detection was performed using anti-Flag and anti-HA antibodies. E-E’. Anti-Srp (green) stained control S2 cells as well as bfc RNAi-treated S2 cells; the nuclei were stained using Hoechst 33342 (blue). Scale bar = 5 μm.

    Article Snippet: The antibodies used in this study were rabbit anti-CRQ (1:400), mouse anti-Bfc (1:400), mouse anti-GFP (CST; 1:500), mouse anti-Flag (Sigma; 1:500), rabbit anti-HA (CST; 1:500), and rabbit anti-Dcp1 (CST, 1:500).

    Techniques: Staining, Fluorescence, Expressing, Isolation, Labeling, Transfection, Immunoprecipitation, Magnetic Beads

    SLIT2 is downregulated in breast cancer cells. (A) The expression of SLIT2 in normal tissue and tumor tissue of most cancers. (B) The expression of SLIT2 in normal tissue and tumor tissue of BRCA. (C) Kaplan–Meier survival curves for SLIT2 in BRCA. (D) IHC staining of SLIT2 in tumor or peri-tumor of BRCA patients. BRCA, breast cancer; IHC, immunohistochemistry. ** p < 0.01,*** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: Enhancer RNA SLIT2 Inhibits Bone Metastasis of Breast Cancer Through Regulating P38 MAPK/c-Fos Signaling Pathway

    doi: 10.3389/fonc.2021.743840

    Figure Lengend Snippet: SLIT2 is downregulated in breast cancer cells. (A) The expression of SLIT2 in normal tissue and tumor tissue of most cancers. (B) The expression of SLIT2 in normal tissue and tumor tissue of BRCA. (C) Kaplan–Meier survival curves for SLIT2 in BRCA. (D) IHC staining of SLIT2 in tumor or peri-tumor of BRCA patients. BRCA, breast cancer; IHC, immunohistochemistry. ** p < 0.01,*** p < 0.001, **** p < 0.0001.

    Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline containing 5% non-fat dried milk at room temperature (RT) and incubated overnight at 4°C with the relevant antibodies: SLIT2 (#47600), GFP (#55494), p-P38 MAPK (#4511), T-P38 MAPK (#8690), p-C-Fos (#5348), T-C-Fos (#2250), and β-actin (#4970) (1:1,000, all from Cell Signaling Technology, USA).

    Techniques: Expressing, Immunohistochemistry

    SLIT2 inhibited the proliferation and migration of breast cancer cells. (A) The efficacy of SLIT2 knockdown or SLIT2 overexpression was examined by qRT-PCR and immunoblotting (B) . (C) Cell proliferation assay was performed by CCK8 in SLIT2 knockdown or SLIT2 overexpression cells. (D, E) Cell cycle assay in SLIT2 knockdown or SLIT2 overexpression cells. (F, G) Cell migration assay was performed by transwell in SLIT2 knockdown or SLIT2 overexpression cells. (H, I) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown or SLIT2 overexpression cells. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 and **** p < 0.0001. CCK8, Cell Counting Kit-8.

    Journal: Frontiers in Oncology

    Article Title: Enhancer RNA SLIT2 Inhibits Bone Metastasis of Breast Cancer Through Regulating P38 MAPK/c-Fos Signaling Pathway

    doi: 10.3389/fonc.2021.743840

    Figure Lengend Snippet: SLIT2 inhibited the proliferation and migration of breast cancer cells. (A) The efficacy of SLIT2 knockdown or SLIT2 overexpression was examined by qRT-PCR and immunoblotting (B) . (C) Cell proliferation assay was performed by CCK8 in SLIT2 knockdown or SLIT2 overexpression cells. (D, E) Cell cycle assay in SLIT2 knockdown or SLIT2 overexpression cells. (F, G) Cell migration assay was performed by transwell in SLIT2 knockdown or SLIT2 overexpression cells. (H, I) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown or SLIT2 overexpression cells. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 and **** p < 0.0001. CCK8, Cell Counting Kit-8.

    Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline containing 5% non-fat dried milk at room temperature (RT) and incubated overnight at 4°C with the relevant antibodies: SLIT2 (#47600), GFP (#55494), p-P38 MAPK (#4511), T-P38 MAPK (#8690), p-C-Fos (#5348), T-C-Fos (#2250), and β-actin (#4970) (1:1,000, all from Cell Signaling Technology, USA).

    Techniques: Migration, Over Expression, Quantitative RT-PCR, Western Blot, Proliferation Assay, Cell Cycle Assay, Cell Migration Assay, Invasion Assay, Standard Deviation, Cell Counting

    SLIT2 regulates breast cancer proliferation and migration through MAPK/FOS signaling pathway. (A) Co-immunoprecipitation (IP) of SLIT2 interacts with MAPK in SLIT2-expressing MCF7 cells. (B) Western blotting of the phospho-MAPK/total-MAPK and phospho-c-Fos/total-c-Fos from three independent experiments in SLIT2 knockdown or SLIT2 overexpression cells with or without p38 MAPK inhibitor SB202190 (10 μM). (C) Cell cycle assay of in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (D) Cell migration assay was performed by transwell in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (E) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown with or without MAPK inhibitor SB202190. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). * p < 0.05, *** p < 0.001, and **** p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: Enhancer RNA SLIT2 Inhibits Bone Metastasis of Breast Cancer Through Regulating P38 MAPK/c-Fos Signaling Pathway

    doi: 10.3389/fonc.2021.743840

    Figure Lengend Snippet: SLIT2 regulates breast cancer proliferation and migration through MAPK/FOS signaling pathway. (A) Co-immunoprecipitation (IP) of SLIT2 interacts with MAPK in SLIT2-expressing MCF7 cells. (B) Western blotting of the phospho-MAPK/total-MAPK and phospho-c-Fos/total-c-Fos from three independent experiments in SLIT2 knockdown or SLIT2 overexpression cells with or without p38 MAPK inhibitor SB202190 (10 μM). (C) Cell cycle assay of in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (D) Cell migration assay was performed by transwell in SLIT2 knockdown cells with or without MAPK inhibitor SB202190. (E) Cell invasion assay was performed by transwell with matrigel in SLIT2 knockdown with or without MAPK inhibitor SB202190. Three independent experiments were performed. All data are presented as mean ± standard deviation (SD). Comparisons between the groups were made by one-way analysis of variance (ANOVA). * p < 0.05, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The membranes were blocked for 1 h in Tris-buffered saline containing 5% non-fat dried milk at room temperature (RT) and incubated overnight at 4°C with the relevant antibodies: SLIT2 (#47600), GFP (#55494), p-P38 MAPK (#4511), T-P38 MAPK (#8690), p-C-Fos (#5348), T-C-Fos (#2250), and β-actin (#4970) (1:1,000, all from Cell Signaling Technology, USA).

    Techniques: Migration, Immunoprecipitation, Expressing, Western Blot, Over Expression, Cell Cycle Assay, Cell Migration Assay, Invasion Assay, Standard Deviation

    Track of implanted cells labeled with GFP signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining using monoclonal antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Unfavorable Contribution of a Tissue-Engineering Cartilage Graft to Osteochondral Defect Repair in Young Rabbits

    doi: 10.3389/fcell.2020.595518

    Figure Lengend Snippet: Track of implanted cells labeled with GFP signal. Immunofluorescence of GFP expression in in vitro tissue constructs (Scale bar: 200 μm) from either dECM or TCP expanded IPFSCs (A) , and six-week ( (B) and 15-week (C) osteochondral defects repaired with PLGA mesh alone (PLGA; n = 6 knees), tissue constructs developed from dECM expanded IPFSCs (dECM; n = 8 knees) or TCP expanded cells (TCP; n = 8 knees), or left untreated (Empty; n = 6 knees) (Scale bar: 1 mm). DAPI served as a counterstain. Immunohistochemical staining using monoclonal antibody showed positive staining (Arrows; ▶) for in vitro tissue constructs (D) but negative staining for in vivo resurfacing cartilage from the tissue construct groups (E) . Scale bar: 100 μm. Hematoxylin served as a counterstain.

    Article Snippet: Sections for GFP detection were treated with citrate unmasking solution for 20 min followed by overnight incubation at 4°C with a monoclonal mouse antibody against GFP (4B10, Cell Signaling Technology, Danvers, MA).

    Techniques: Labeling, Immunofluorescence, Expressing, In Vitro, Construct, Immunohistochemical staining, Staining, Negative Staining, In Vivo

    Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Article Snippet: To assess levels of autophagic flux in peritoneal tissue in vivo study, we used LC3-GFP mice and anti-mouse GFP monoclonal antibodies (Cell Signaling, Danvers, MA). α-SMA, mesothein and GFP positive cells were visualized by incubating antibody-stained sections with DAB (DAKO), respectively.

    Techniques: Expressing, Inhibition, Incubation

    Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.

    Article Snippet: To assess levels of autophagic flux in peritoneal tissue in vivo study, we used LC3-GFP mice and anti-mouse GFP monoclonal antibodies (Cell Signaling, Danvers, MA). α-SMA, mesothein and GFP positive cells were visualized by incubating antibody-stained sections with DAB (DAKO), respectively.

    Techniques: In Vivo, In Vitro, Incubation

    Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose promoted the production of autophagosomes and suppressed MMT in peritoneal mesothelial cells. ( a , b ) PMCs were pre-treated with baffilomycin A1 for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 1 h. Trehalose increased the ratio of LC3-II to LC3-I protein expression as compared with vehicle treatment. Data are expressed as mean density of LC3-II bands relative to LC3-I bands ± SEM expression (n = 3 cell preparation/group). ( c ) The inhibition of autophagy by bafilomycin A1 upregulated Snail1 protein expression even under the incubation with trehalose (n = 3 cell preparation/group). Data are expressed as mean ± SEM. ( d , e ) The effects of trehalose on E-cadherin protein expression in PMCs (n = 3 cell preparation/group). Data are expressed as mean density of E-cadherin bands relative to β-actin bands ± SEM. ( f ) The localization of GFP protein in the peritoneum in LC3-GFP mice. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( g ) Numbers of GFP-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Article Snippet: To identify proliferating fibroblasts, peritoneal sections from Col1α 2 -enhanced GFP mice were co-stained with anti-mouse GFP monoclonal antibody (Cell Signaling) and anti-mouse PCNA monoclonal antibody (Abcam), using an M.O.M. kit (Vector laboratories, Burlingame, CA).

    Techniques: Expressing, Inhibition, Incubation

    Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose attenuated collagen producing fibroblast accumulation and proliferation in in vivo and in vitro studies. ( a ) Peritoneal accumulation of proliferating fibroblasts after CG challenges. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of sub-mesothelial GFP + cells (fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( c ) Numbers of sub-mesothelial GFP + PCNA + cells (proliferating fibroblasts), expressed as mean number per HPF (n = 3 mice/group). ( d ) Percentage of sub-mesothelial fibroblasts that are proliferating (n = 3 mice/group). ( e ) NIH3T3 fibroblasts were pre-treated with control medium or trehalose at indicated concentrations for 30 min, and then incubated with TGF-β 1 . BrdU proliferation assays were performed after incubation with TGF-β 1 for 48 h (n = 3 cells preparations/group), and expressed as mean OD value (OD 370–492 ). Data are expressed as means ± SEM.

    Article Snippet: To identify proliferating fibroblasts, peritoneal sections from Col1α 2 -enhanced GFP mice were co-stained with anti-mouse GFP monoclonal antibody (Cell Signaling) and anti-mouse PCNA monoclonal antibody (Abcam), using an M.O.M. kit (Vector laboratories, Burlingame, CA).

    Techniques: In Vivo, In Vitro, Incubation