cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd63
    Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    cd63 - by Bioz Stars, 2023-01
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    cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd63
    Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    rabbit monoclonal anti cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti cd63
    ( A ) Schematic representation of EV preparation and experimental workflow for miRNA profiling and EV characterization. ( B ) Purified EVs from healthy control (HC) and pulmonary arterial hypertension (PAH) patients were subjected to flow cytometry to detect expression levels of exosome-specific marker <t>CD63.</t> Results represent marker count/50,000 events and are given as mean ± S.E.M. ( n = 10 per group), comparing PAH versus HC. ( C ) Representative immunoblot of <t>CD63</t> from EVs from HC and PAH patients. ( D ) Representative transmission electron micrographs of purified EVs from HC and PAH patients with uranyl acetate negative staining (scale bar 200–1000 nm) ( n = 5 per group; 10–20 images were taken per sample).
    Rabbit Monoclonal Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti cd63 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "MircoRNA in Extracellular Vesicles from Patients with Pulmonary Arterial Hypertension Alters Endothelial Angiogenic Response"

    Article Title: MircoRNA in Extracellular Vesicles from Patients with Pulmonary Arterial Hypertension Alters Endothelial Angiogenic Response

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms231911964

    ( A ) Schematic representation of EV preparation and experimental workflow for miRNA profiling and EV characterization. ( B ) Purified EVs from healthy control (HC) and pulmonary arterial hypertension (PAH) patients were subjected to flow cytometry to detect expression levels of exosome-specific marker CD63. Results represent marker count/50,000 events and are given as mean ± S.E.M. ( n = 10 per group), comparing PAH versus HC. ( C ) Representative immunoblot of CD63 from EVs from HC and PAH patients. ( D ) Representative transmission electron micrographs of purified EVs from HC and PAH patients with uranyl acetate negative staining (scale bar 200–1000 nm) ( n = 5 per group; 10–20 images were taken per sample).
    Figure Legend Snippet: ( A ) Schematic representation of EV preparation and experimental workflow for miRNA profiling and EV characterization. ( B ) Purified EVs from healthy control (HC) and pulmonary arterial hypertension (PAH) patients were subjected to flow cytometry to detect expression levels of exosome-specific marker CD63. Results represent marker count/50,000 events and are given as mean ± S.E.M. ( n = 10 per group), comparing PAH versus HC. ( C ) Representative immunoblot of CD63 from EVs from HC and PAH patients. ( D ) Representative transmission electron micrographs of purified EVs from HC and PAH patients with uranyl acetate negative staining (scale bar 200–1000 nm) ( n = 5 per group; 10–20 images were taken per sample).

    Techniques Used: Purification, Flow Cytometry, Expressing, Marker, Western Blot, Transmission Assay, Negative Staining

    mouse anti vimentin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti vimentin
    The presence of iCAFs in the stroma of 3D vesical models induces the proliferation and infiltration of urothelial cells. ( A ) Representative fluorescent images of the HFs-derived and iCAFs-derived models stained <t>for</t> <t>Ki67</t> (green). Nuclei were counterstained with Hoechst (blue). ( B ) Corresponding quantification in relative fluorescence units (RFU) of the Ki67 signal. N = 4 independent biological replicates. ( C ) Representative fluorescent images of the HFs-derived and iCAFs-derived models of laminin-332. ( D ) Representative fluorescent images of <t>vimentin-</t> and epithelium-specific cytokeratin marker AE1/AE3 in the HFs-derived and iCAFs-derived constructs. The images show infiltration of epithelial cells within the stroma in the iCAFs-derived stroma. E = epithelium; S = stroma. The data are represented as mean ± SEM. **** p < 0.0001.
    Mouse Anti Vimentin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti vimentin/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    mouse anti vimentin - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT Transition"

    Article Title: Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT Transition

    Journal: Cancers

    doi: 10.3390/cancers14153810

    The presence of iCAFs in the stroma of 3D vesical models induces the proliferation and infiltration of urothelial cells. ( A ) Representative fluorescent images of the HFs-derived and iCAFs-derived models stained for Ki67 (green). Nuclei were counterstained with Hoechst (blue). ( B ) Corresponding quantification in relative fluorescence units (RFU) of the Ki67 signal. N = 4 independent biological replicates. ( C ) Representative fluorescent images of the HFs-derived and iCAFs-derived models of laminin-332. ( D ) Representative fluorescent images of vimentin- and epithelium-specific cytokeratin marker AE1/AE3 in the HFs-derived and iCAFs-derived constructs. The images show infiltration of epithelial cells within the stroma in the iCAFs-derived stroma. E = epithelium; S = stroma. The data are represented as mean ± SEM. **** p < 0.0001.
    Figure Legend Snippet: The presence of iCAFs in the stroma of 3D vesical models induces the proliferation and infiltration of urothelial cells. ( A ) Representative fluorescent images of the HFs-derived and iCAFs-derived models stained for Ki67 (green). Nuclei were counterstained with Hoechst (blue). ( B ) Corresponding quantification in relative fluorescence units (RFU) of the Ki67 signal. N = 4 independent biological replicates. ( C ) Representative fluorescent images of the HFs-derived and iCAFs-derived models of laminin-332. ( D ) Representative fluorescent images of vimentin- and epithelium-specific cytokeratin marker AE1/AE3 in the HFs-derived and iCAFs-derived constructs. The images show infiltration of epithelial cells within the stroma in the iCAFs-derived stroma. E = epithelium; S = stroma. The data are represented as mean ± SEM. **** p < 0.0001.

    Techniques Used: Derivative Assay, Staining, Fluorescence, Marker, Construct

    rabbit anti cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti cd63
    Characterization of MSC-EVs. A – B Flow cytometry results showed 73.4% and 74.5% of EVs were positive for <t>CD63</t> A and CD81 (B). C Particle size analysis of the EVs showing the distribution of EVs with different sizes. Around 75% of the particles had the size of exosomes, i.e., 30–200 nm. D Morphologies of two typical EVs taken by transmission electron microscopy (indicated by arrows). E. Western blotting showing the expression of EVs’ markers (CD9, CD63, Hsp70 and ALIX) in two batches of exosomes, EVs-1 and EVs-2, with lower concentration
    Rabbit Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cd63 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axis"

    Article Title: Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axis

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-022-03100-x

    Characterization of MSC-EVs. A – B Flow cytometry results showed 73.4% and 74.5% of EVs were positive for CD63 A and CD81 (B). C Particle size analysis of the EVs showing the distribution of EVs with different sizes. Around 75% of the particles had the size of exosomes, i.e., 30–200 nm. D Morphologies of two typical EVs taken by transmission electron microscopy (indicated by arrows). E. Western blotting showing the expression of EVs’ markers (CD9, CD63, Hsp70 and ALIX) in two batches of exosomes, EVs-1 and EVs-2, with lower concentration
    Figure Legend Snippet: Characterization of MSC-EVs. A – B Flow cytometry results showed 73.4% and 74.5% of EVs were positive for CD63 A and CD81 (B). C Particle size analysis of the EVs showing the distribution of EVs with different sizes. Around 75% of the particles had the size of exosomes, i.e., 30–200 nm. D Morphologies of two typical EVs taken by transmission electron microscopy (indicated by arrows). E. Western blotting showing the expression of EVs’ markers (CD9, CD63, Hsp70 and ALIX) in two batches of exosomes, EVs-1 and EVs-2, with lower concentration

    Techniques Used: Flow Cytometry, Particle Size Analysis, Transmission Assay, Electron Microscopy, Western Blot, Expressing, Concentration Assay

    anti cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cd63
    Inhibition of NKG2D expression by exosomal MICA/B. (A) IMR-32 cells were treated with 2 μM MLN8237 or 0.5 μM DOX for 72 h. No treatment was used as a control, and exosomes were isolated by ultracentrifugation. Western blot was used to evaluate <t>CD63</t> and TSG101 levels in whole cell lysates of the cells or isolated exosomes from supernatants. (B) Representative EM image of exosomes and showing the typical cup-shaped morphology. Scale bar, 100 nm. (C) Analysis of particle size distribution and concentration in exosomes. (D) IMR-32 cells were treated with 2 μM MLN8237 for 72 h, no treatment was used as a control. The expression of MICA/B in IMR-32 cells and exosomes were determined by flow cytometry, and exosomes were pretreated by coated latex microbeads. (E) Normal human peripheral blood NK cells were used as a control. Exosomes isolated from untreated IMR-32 cells, and MLN8237-treated cells were co-cultured with NK cells for 24 h. Co-cultures were spiked with the MICA/B antibody, and NKG2D expression was detected by flow cytometry.
    Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd63 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells"

    Article Title: Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2022.829404

    Inhibition of NKG2D expression by exosomal MICA/B. (A) IMR-32 cells were treated with 2 μM MLN8237 or 0.5 μM DOX for 72 h. No treatment was used as a control, and exosomes were isolated by ultracentrifugation. Western blot was used to evaluate CD63 and TSG101 levels in whole cell lysates of the cells or isolated exosomes from supernatants. (B) Representative EM image of exosomes and showing the typical cup-shaped morphology. Scale bar, 100 nm. (C) Analysis of particle size distribution and concentration in exosomes. (D) IMR-32 cells were treated with 2 μM MLN8237 for 72 h, no treatment was used as a control. The expression of MICA/B in IMR-32 cells and exosomes were determined by flow cytometry, and exosomes were pretreated by coated latex microbeads. (E) Normal human peripheral blood NK cells were used as a control. Exosomes isolated from untreated IMR-32 cells, and MLN8237-treated cells were co-cultured with NK cells for 24 h. Co-cultures were spiked with the MICA/B antibody, and NKG2D expression was detected by flow cytometry.
    Figure Legend Snippet: Inhibition of NKG2D expression by exosomal MICA/B. (A) IMR-32 cells were treated with 2 μM MLN8237 or 0.5 μM DOX for 72 h. No treatment was used as a control, and exosomes were isolated by ultracentrifugation. Western blot was used to evaluate CD63 and TSG101 levels in whole cell lysates of the cells or isolated exosomes from supernatants. (B) Representative EM image of exosomes and showing the typical cup-shaped morphology. Scale bar, 100 nm. (C) Analysis of particle size distribution and concentration in exosomes. (D) IMR-32 cells were treated with 2 μM MLN8237 for 72 h, no treatment was used as a control. The expression of MICA/B in IMR-32 cells and exosomes were determined by flow cytometry, and exosomes were pretreated by coated latex microbeads. (E) Normal human peripheral blood NK cells were used as a control. Exosomes isolated from untreated IMR-32 cells, and MLN8237-treated cells were co-cultured with NK cells for 24 h. Co-cultures were spiked with the MICA/B antibody, and NKG2D expression was detected by flow cytometry.

    Techniques Used: Inhibition, Expressing, Isolation, Western Blot, Concentration Assay, Flow Cytometry, Cell Culture

    cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd63
    Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd63 - by Bioz Stars, 2023-01
    94/100 stars

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    cd63  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd63
    Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd63 - by Bioz Stars, 2023-01
    94/100 stars

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    cd63 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd63 antibody
    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, <t>CD63</t> and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes
    Cd63 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63 antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd63 antibody - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome"

    Article Title: Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome

    Journal: Reproductive Biology and Endocrinology : RB&E

    doi: 10.1186/s12958-021-00749-6

    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes
    Figure Legend Snippet: Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes

    Techniques Used: Western Blot, Isolation

    cd63 d4i1x rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd63 d4i1x rabbit mab
    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, <t>CD63</t> and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes
    Cd63 D4i1x Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63 d4i1x rabbit mab/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd63 d4i1x rabbit mab - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome"

    Article Title: Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome

    Journal: Reproductive Biology and Endocrinology : RB&E

    doi: 10.1186/s12958-021-00749-6

    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes
    Figure Legend Snippet: Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes

    Techniques Used: Western Blot, Isolation

    rabbit anti cd63  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc rabbit anti cd63
    Characteristic of MSC-derived EVs. (A) Representative TEM images of MSC-derived EVs. Scale bar, 100 nm. (B) NTA analysis of MSC-derived EVs. (C) Representative 3-dimensional confocal images showing microglia (Iba1, green) engulfing MSC-derived EVs (red). Scale bar, 10 μm. (D) Immunoblot (D) and quantitative analysis (E) of CD9, CD29, <t>CD63,</t> CD105, CD73, IL-10, GAPDH in MSCs, MSC-derived EVs, and MSC-derived EVs with IL-10 knockdown. Data are presented as the mean ± standard deviation (*** p < 0.001).
    Rabbit Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cd63/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    rabbit anti cd63 - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Mesenchymal-Stem-Cell–Derived Extracellular Vesicles Mitigate Trained Immunity in the Brain"

    Article Title: Mesenchymal-Stem-Cell–Derived Extracellular Vesicles Mitigate Trained Immunity in the Brain

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2020.599058

    Characteristic of MSC-derived EVs. (A) Representative TEM images of MSC-derived EVs. Scale bar, 100 nm. (B) NTA analysis of MSC-derived EVs. (C) Representative 3-dimensional confocal images showing microglia (Iba1, green) engulfing MSC-derived EVs (red). Scale bar, 10 μm. (D) Immunoblot (D) and quantitative analysis (E) of CD9, CD29, CD63, CD105, CD73, IL-10, GAPDH in MSCs, MSC-derived EVs, and MSC-derived EVs with IL-10 knockdown. Data are presented as the mean ± standard deviation (*** p < 0.001).
    Figure Legend Snippet: Characteristic of MSC-derived EVs. (A) Representative TEM images of MSC-derived EVs. Scale bar, 100 nm. (B) NTA analysis of MSC-derived EVs. (C) Representative 3-dimensional confocal images showing microglia (Iba1, green) engulfing MSC-derived EVs (red). Scale bar, 10 μm. (D) Immunoblot (D) and quantitative analysis (E) of CD9, CD29, CD63, CD105, CD73, IL-10, GAPDH in MSCs, MSC-derived EVs, and MSC-derived EVs with IL-10 knockdown. Data are presented as the mean ± standard deviation (*** p < 0.001).

    Techniques Used: Derivative Assay, Western Blot, Standard Deviation

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    Cell Signaling Technology Inc cd63
    Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63/product/Cell Signaling Technology Inc
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    cd63 - by Bioz Stars, 2023-01
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    Cell Signaling Technology Inc rabbit monoclonal anti cd63
    ( A ) Schematic representation of EV preparation and experimental workflow for miRNA profiling and EV characterization. ( B ) Purified EVs from healthy control (HC) and pulmonary arterial hypertension (PAH) patients were subjected to flow cytometry to detect expression levels of exosome-specific marker <t>CD63.</t> Results represent marker count/50,000 events and are given as mean ± S.E.M. ( n = 10 per group), comparing PAH versus HC. ( C ) Representative immunoblot of <t>CD63</t> from EVs from HC and PAH patients. ( D ) Representative transmission electron micrographs of purified EVs from HC and PAH patients with uranyl acetate negative staining (scale bar 200–1000 nm) ( n = 5 per group; 10–20 images were taken per sample).
    Rabbit Monoclonal Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cd63/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc mouse anti vimentin
    The presence of iCAFs in the stroma of 3D vesical models induces the proliferation and infiltration of urothelial cells. ( A ) Representative fluorescent images of the HFs-derived and iCAFs-derived models stained <t>for</t> <t>Ki67</t> (green). Nuclei were counterstained with Hoechst (blue). ( B ) Corresponding quantification in relative fluorescence units (RFU) of the Ki67 signal. N = 4 independent biological replicates. ( C ) Representative fluorescent images of the HFs-derived and iCAFs-derived models of laminin-332. ( D ) Representative fluorescent images of <t>vimentin-</t> and epithelium-specific cytokeratin marker AE1/AE3 in the HFs-derived and iCAFs-derived constructs. The images show infiltration of epithelial cells within the stroma in the iCAFs-derived stroma. E = epithelium; S = stroma. The data are represented as mean ± SEM. **** p < 0.0001.
    Mouse Anti Vimentin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti vimentin/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc rabbit anti cd63
    Characterization of MSC-EVs. A – B Flow cytometry results showed 73.4% and 74.5% of EVs were positive for <t>CD63</t> A and CD81 (B). C Particle size analysis of the EVs showing the distribution of EVs with different sizes. Around 75% of the particles had the size of exosomes, i.e., 30–200 nm. D Morphologies of two typical EVs taken by transmission electron microscopy (indicated by arrows). E. Western blotting showing the expression of EVs’ markers (CD9, CD63, Hsp70 and ALIX) in two batches of exosomes, EVs-1 and EVs-2, with lower concentration
    Rabbit Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cd63/product/Cell Signaling Technology Inc
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    Inhibition of NKG2D expression by exosomal MICA/B. (A) IMR-32 cells were treated with 2 μM MLN8237 or 0.5 μM DOX for 72 h. No treatment was used as a control, and exosomes were isolated by ultracentrifugation. Western blot was used to evaluate <t>CD63</t> and TSG101 levels in whole cell lysates of the cells or isolated exosomes from supernatants. (B) Representative EM image of exosomes and showing the typical cup-shaped morphology. Scale bar, 100 nm. (C) Analysis of particle size distribution and concentration in exosomes. (D) IMR-32 cells were treated with 2 μM MLN8237 for 72 h, no treatment was used as a control. The expression of MICA/B in IMR-32 cells and exosomes were determined by flow cytometry, and exosomes were pretreated by coated latex microbeads. (E) Normal human peripheral blood NK cells were used as a control. Exosomes isolated from untreated IMR-32 cells, and MLN8237-treated cells were co-cultured with NK cells for 24 h. Co-cultures were spiked with the MICA/B antibody, and NKG2D expression was detected by flow cytometry.
    Anti Cd63, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd63/product/Cell Signaling Technology Inc
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    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, <t>CD63</t> and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes
    Cd63 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63 antibody/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc cd63 d4i1x rabbit mab
    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, <t>CD63</t> and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes
    Cd63 D4i1x Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd63 d4i1x rabbit mab/product/Cell Signaling Technology Inc
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    Image Search Results


    ( A ) Schematic representation of EV preparation and experimental workflow for miRNA profiling and EV characterization. ( B ) Purified EVs from healthy control (HC) and pulmonary arterial hypertension (PAH) patients were subjected to flow cytometry to detect expression levels of exosome-specific marker CD63. Results represent marker count/50,000 events and are given as mean ± S.E.M. ( n = 10 per group), comparing PAH versus HC. ( C ) Representative immunoblot of CD63 from EVs from HC and PAH patients. ( D ) Representative transmission electron micrographs of purified EVs from HC and PAH patients with uranyl acetate negative staining (scale bar 200–1000 nm) ( n = 5 per group; 10–20 images were taken per sample).

    Journal: International Journal of Molecular Sciences

    Article Title: MircoRNA in Extracellular Vesicles from Patients with Pulmonary Arterial Hypertension Alters Endothelial Angiogenic Response

    doi: 10.3390/ijms231911964

    Figure Lengend Snippet: ( A ) Schematic representation of EV preparation and experimental workflow for miRNA profiling and EV characterization. ( B ) Purified EVs from healthy control (HC) and pulmonary arterial hypertension (PAH) patients were subjected to flow cytometry to detect expression levels of exosome-specific marker CD63. Results represent marker count/50,000 events and are given as mean ± S.E.M. ( n = 10 per group), comparing PAH versus HC. ( C ) Representative immunoblot of CD63 from EVs from HC and PAH patients. ( D ) Representative transmission electron micrographs of purified EVs from HC and PAH patients with uranyl acetate negative staining (scale bar 200–1000 nm) ( n = 5 per group; 10–20 images were taken per sample).

    Article Snippet: For immunoblot analysis, the following antibodies were used: rabbit monoclonal anti-CD63 (D4I1X; Cell Signaling, Frankfurt am Main, Germany), rabbit monoclonal anti- NF-κB p65 (D14E12; Cell Signaling), rabbit monoclonal anti-PTEN (D4.3; Cell Signaling), rabbit monoclonal anti-TAK1 (D94D7; Cell Signaling), goat polyclonal anti-human VEGF-165 (VEGF-A; Cell Signaling), mouse monoclonal anti–β-actin (Sigma Aldrich, St. Louis, MI, USA), and secondary anti-mouse or anti-rabbit fluorescently labeled antibody (LI-COR).

    Techniques: Purification, Flow Cytometry, Expressing, Marker, Western Blot, Transmission Assay, Negative Staining

    The presence of iCAFs in the stroma of 3D vesical models induces the proliferation and infiltration of urothelial cells. ( A ) Representative fluorescent images of the HFs-derived and iCAFs-derived models stained for Ki67 (green). Nuclei were counterstained with Hoechst (blue). ( B ) Corresponding quantification in relative fluorescence units (RFU) of the Ki67 signal. N = 4 independent biological replicates. ( C ) Representative fluorescent images of the HFs-derived and iCAFs-derived models of laminin-332. ( D ) Representative fluorescent images of vimentin- and epithelium-specific cytokeratin marker AE1/AE3 in the HFs-derived and iCAFs-derived constructs. The images show infiltration of epithelial cells within the stroma in the iCAFs-derived stroma. E = epithelium; S = stroma. The data are represented as mean ± SEM. **** p < 0.0001.

    Journal: Cancers

    Article Title: Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT Transition

    doi: 10.3390/cancers14153810

    Figure Lengend Snippet: The presence of iCAFs in the stroma of 3D vesical models induces the proliferation and infiltration of urothelial cells. ( A ) Representative fluorescent images of the HFs-derived and iCAFs-derived models stained for Ki67 (green). Nuclei were counterstained with Hoechst (blue). ( B ) Corresponding quantification in relative fluorescence units (RFU) of the Ki67 signal. N = 4 independent biological replicates. ( C ) Representative fluorescent images of the HFs-derived and iCAFs-derived models of laminin-332. ( D ) Representative fluorescent images of vimentin- and epithelium-specific cytokeratin marker AE1/AE3 in the HFs-derived and iCAFs-derived constructs. The images show infiltration of epithelial cells within the stroma in the iCAFs-derived stroma. E = epithelium; S = stroma. The data are represented as mean ± SEM. **** p < 0.0001.

    Article Snippet: Primary antibodies used were as follows: Rabbit anti-YAP1 (#14074, Cell signaling Technology, Whitby, ON, Canada); Mouse anti-vimentin (#550513), Mouse anti-E-Cadherin (#610181) and Mouse anti-Ki67 (#556003) were from BD BIOSCIENCES (Franklin Lakes, NJ, USA); Rabbit anti-ZEB1 (#ABD53), Rabbit anti-total Fibronectin (#F3648), Mouse anti-laminin-V (#MAB19562), and Mouse pan-anti-Cytokeratin AE1/AE3 (#MAB3412) were from MilliporeSigma (Billerica, MA, USA); Rabbit anti-vimentin (#ab45939), Rabbit anti-MMP9 (#ab76003) were from Abcam (San Francisco, CA, USA).

    Techniques: Derivative Assay, Staining, Fluorescence, Marker, Construct

    Characterization of MSC-EVs. A – B Flow cytometry results showed 73.4% and 74.5% of EVs were positive for CD63 A and CD81 (B). C Particle size analysis of the EVs showing the distribution of EVs with different sizes. Around 75% of the particles had the size of exosomes, i.e., 30–200 nm. D Morphologies of two typical EVs taken by transmission electron microscopy (indicated by arrows). E. Western blotting showing the expression of EVs’ markers (CD9, CD63, Hsp70 and ALIX) in two batches of exosomes, EVs-1 and EVs-2, with lower concentration

    Journal: Stem Cell Research & Therapy

    Article Title: Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axis

    doi: 10.1186/s13287-022-03100-x

    Figure Lengend Snippet: Characterization of MSC-EVs. A – B Flow cytometry results showed 73.4% and 74.5% of EVs were positive for CD63 A and CD81 (B). C Particle size analysis of the EVs showing the distribution of EVs with different sizes. Around 75% of the particles had the size of exosomes, i.e., 30–200 nm. D Morphologies of two typical EVs taken by transmission electron microscopy (indicated by arrows). E. Western blotting showing the expression of EVs’ markers (CD9, CD63, Hsp70 and ALIX) in two batches of exosomes, EVs-1 and EVs-2, with lower concentration

    Article Snippet: The primary antibodies used included mouse anti-IL-1β (1:1000, CST, #12,242), mouse anti-IκBα (1:1000, CST, #4814), rabbit anti-Phospho-IκBα (Ser32) (1:1000, CST, #5209), mouse anti-NF-κB p65 (1:1000, CST, #8242), rabbit anti-CD9(1:1000, CST, #13403S), rabbit anti-CD63 (1:1000, CST, #55051S), rabbit anti-Hsp70 (1:1000, CST, #4873S) and rabbit anti-ALIX (1:1000, CST, #92880S).

    Techniques: Flow Cytometry, Particle Size Analysis, Transmission Assay, Electron Microscopy, Western Blot, Expressing, Concentration Assay

    Inhibition of NKG2D expression by exosomal MICA/B. (A) IMR-32 cells were treated with 2 μM MLN8237 or 0.5 μM DOX for 72 h. No treatment was used as a control, and exosomes were isolated by ultracentrifugation. Western blot was used to evaluate CD63 and TSG101 levels in whole cell lysates of the cells or isolated exosomes from supernatants. (B) Representative EM image of exosomes and showing the typical cup-shaped morphology. Scale bar, 100 nm. (C) Analysis of particle size distribution and concentration in exosomes. (D) IMR-32 cells were treated with 2 μM MLN8237 for 72 h, no treatment was used as a control. The expression of MICA/B in IMR-32 cells and exosomes were determined by flow cytometry, and exosomes were pretreated by coated latex microbeads. (E) Normal human peripheral blood NK cells were used as a control. Exosomes isolated from untreated IMR-32 cells, and MLN8237-treated cells were co-cultured with NK cells for 24 h. Co-cultures were spiked with the MICA/B antibody, and NKG2D expression was detected by flow cytometry.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells

    doi: 10.3389/fcell.2022.829404

    Figure Lengend Snippet: Inhibition of NKG2D expression by exosomal MICA/B. (A) IMR-32 cells were treated with 2 μM MLN8237 or 0.5 μM DOX for 72 h. No treatment was used as a control, and exosomes were isolated by ultracentrifugation. Western blot was used to evaluate CD63 and TSG101 levels in whole cell lysates of the cells or isolated exosomes from supernatants. (B) Representative EM image of exosomes and showing the typical cup-shaped morphology. Scale bar, 100 nm. (C) Analysis of particle size distribution and concentration in exosomes. (D) IMR-32 cells were treated with 2 μM MLN8237 for 72 h, no treatment was used as a control. The expression of MICA/B in IMR-32 cells and exosomes were determined by flow cytometry, and exosomes were pretreated by coated latex microbeads. (E) Normal human peripheral blood NK cells were used as a control. Exosomes isolated from untreated IMR-32 cells, and MLN8237-treated cells were co-cultured with NK cells for 24 h. Co-cultures were spiked with the MICA/B antibody, and NKG2D expression was detected by flow cytometry.

    Article Snippet: For western blot, 20 µg of each protein was electrophoresed and then transferred to a polyvinylidene fluoride membrane, which was incubated with Tris-buffered saline containing Tween-20 plus 5% skim milk powder for 1 h. Protein bands were isolated according to the molecular weight marker (26616, Thermo Fisher Scientific, Waltham, MA, United States) and incubated with the following primary antibodies: anti-CD63 (1:1000, 55051S, Cell Signaling Technology, Danvers, MA, United States), tumor susceptibility gene 101 (TSG101, 1:1000, 28405S, Cell Signaling Technology), disintegrin and metalloproteinase domain-containing 10 (ADAM10, 1:1000, GTX104940, GeneTex, Irvine, CA, United States), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:1000, GTX100118, GeneTex).

    Techniques: Inhibition, Expressing, Isolation, Western Blot, Concentration Assay, Flow Cytometry, Cell Culture

    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes

    Journal: Reproductive Biology and Endocrinology : RB&E

    Article Title: Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome

    doi: 10.1186/s12958-021-00749-6

    Figure Lengend Snippet: Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes

    Article Snippet: CD9 antibody: CD9 (D8O1A) Rabbit mAb (#13174, Cell signaling technology); CD63 antibody: CD63 (D4I1X) Rabbit mAb (#55051, Cell signaling technology); TSG101 (ab125011, abcam); Calnexin (#2433, Cell signaling technology).

    Techniques: Western Blot, Isolation

    Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes

    Journal: Reproductive Biology and Endocrinology : RB&E

    Article Title: Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome

    doi: 10.1186/s12958-021-00749-6

    Figure Lengend Snippet: Quality control of extracted follicular fluid exosomes. ( a ) Electron microscopic images of extracted exosomes revealed cup-shaped structures with a diameter of about 30–150 nm. Scale bar: 100 nm. ( b ) Western blotting revealed CD9, CD63 and TSG101 proteins in exosome samples. ( c ) Nanoparticle tracking analysis (NTA) revealed the diameter of isolated extracellular vesicles (EVs) is consistent with that of exosomes

    Article Snippet: CD9 antibody: CD9 (D8O1A) Rabbit mAb (#13174, Cell signaling technology); CD63 antibody: CD63 (D4I1X) Rabbit mAb (#55051, Cell signaling technology); TSG101 (ab125011, abcam); Calnexin (#2433, Cell signaling technology).

    Techniques: Western Blot, Isolation