Journal: Molecules

Article Title: Combination Antitumor Effect of Sorafenib via Calcium-Dependent Deactivation of Focal Adhesion Kinase Targeting Colorectal Cancer Cells

doi: 10.3390/molecules25225299

Figure Lengend Snippet: Confirmation of the protein expression of phospho-focal adhesion kinase (pFAK) and the sub-signaling pathways following deactivation of the calcium-dependent steroid receptor coactivator (Src). ( A ) Lactate calcium salt (CaLa)-induced increase in the intracellular calcium influx. ( B ) Immunoblot analysis of pFAK and the sub-signaling pathways in CRC cells. ( C – F ) Enzyme-linked immunosorbent assay (ELISA) assay and quantitative analysis for phospho-(p) Src [pTyr 416 ], pFAK [pTyr 397 ], pAkt [pSer 473 ], and p-mammalian target of rapamycin (mTOR) [pSer 2448 ] sensitivity in CRC cells. ** p < 0.001 vs. Control. Results are mean ± SD.

Article Snippet: Quantitative analysis was performed using a human pSrc ELISA kit (Cell Signaling, Danvers, MA, USA) and human pFAK, pAkt, and p-mTOR ELISA kits (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Gentamicin Targets Acid Sphingomyelinase in Cancer: The Case of the Human Gastric Cancer NCI-N87 Cells

doi: 10.3390/ijms20184375

Figure Lengend Snippet: Effect of gentamicin on AKT/p-AKP protein expression. Experiments were performed in control and experimental NCI-N87 cells collected 24 h after 1.5 mM GM treatment. ( a ) Immunoblots of proteins were probed with anti-AKT and p-AKT and visualized by enhanced chemiluminescence (ECL). β-tubulin was used as loading control. ( b ) The area density was evaluated by densitometry scanning and analysis with ImageJ program, the data represent the mean ± SD of three experiments performed in duplicate (significance, * p < 0.001 versus control sample).

Article Snippet: Anti-AKT and phosphor-AKT (p-AKT) were from Cell Signaling Technology—EuroClone (Milano, Italy).

Techniques: Expressing, Western Blot

Journal: Cell Metabolism

Article Title: Integrated Pharmacodynamic Analysis Identifies Two Metabolic Adaption Pathways to Metformin in Breast Cancer

doi: 10.1016/j.cmet.2018.08.021

Figure Lengend Snippet:

Article Snippet: pAKT ser473 residue , Cell Signaling Technologies , RRID: AB_2315049.

Techniques: Software, Imaging