hdac1 6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hdac1 6
    Hdac1 6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hdac1 6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hdac1 6
    Hdac1 6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10e2 5356 rrid ab 10612242  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 10e2 5356 rrid ab 10612242
    10e2 5356 Rrid Ab 10612242, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse mab10e2 hdac1 5356s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse mab10e2 hdac1 5356s
    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and <t>HDAC1</t> (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
    Mouse Mab10e2 Hdac1 5356s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells"

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

    Journal: bioRxiv

    doi: 10.1101/619080

    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
    Figure Legend Snippet: (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.

    Techniques Used: Western Blot, Methylation, Stable Transfection, Expressing

    anti hdac1 5356 mouse monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti hdac1 5356s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti hdac1 5356s
    Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and <t>Hdac1,</t> in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.
    Anti Hdac1 5356s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Identification of early indicators of altered metabolism in normal development using a rodent model system"

    Article Title: Identification of early indicators of altered metabolism in normal development using a rodent model system

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.031815

    Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and Hdac1, in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.
    Figure Legend Snippet: Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and Hdac1, in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.

    Techniques Used: Western Blot, Co-Immunoprecipitation Assay

    Possible mechanism by which embryonic growth is coordinated and correlated with the maternal environment. Elevated Dnmt1 expression might be increasing methylation in the promoters of genes, resulting in suppression of the transcription of genes required for normal growth. In addition, the formation of the Dnmt1 repressive complex with Rb, E2f1 and Hdac1 might slow down cell cycle progression. These processes, probably working together, could be responsible for the growth reduction observed in the birth weight of LBW pups.
    Figure Legend Snippet: Possible mechanism by which embryonic growth is coordinated and correlated with the maternal environment. Elevated Dnmt1 expression might be increasing methylation in the promoters of genes, resulting in suppression of the transcription of genes required for normal growth. In addition, the formation of the Dnmt1 repressive complex with Rb, E2f1 and Hdac1 might slow down cell cycle progression. These processes, probably working together, could be responsible for the growth reduction observed in the birth weight of LBW pups.

    Techniques Used: Expressing, Methylation

    anti hdac1 5356 mouse monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of <t>HDAC1</t> and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells"

    Article Title: CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

    Journal: Journal of Clinical Biochemistry and Nutrition

    doi: 10.3164/jcbn.16-109

    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Figure Legend Snippet: Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.
    Figure Legend Snippet: Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.

    Techniques Used: Binding Assay, Negative Control, Western Blot

    Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.
    Figure Legend Snippet: Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.

    Techniques Used: Expressing

    hdac1 5356  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hdac1 5356
    Hdac1 5356, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and <t>HDAC1</t> (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
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    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and <t>HDAC1</t> (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and <t>Hdac1,</t> in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.
    Anti Hdac1 5356s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc hdac1 5356
    Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and <t>Hdac1,</t> in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.
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    Image Search Results


    (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.

    Journal: bioRxiv

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

    doi: 10.1101/619080

    Figure Lengend Snippet: (A) Western blot analysis of PRC2 core complex subunits (SUZ12, RBAP48, EED and EZH2), EZHIP and HDAC1 (loading control) on U2OS nuclear extracts WT, EED -/- and EZHIP -/- . (B) Western blot analysis of H3K27 methylation: H3K27me1, H3K27me2, H3K27me3, H3K27ac and, H3 and H4 (loading controls). (C) Scheme representing EZHIP mutants (left panel) stably reintroduced in U2OS EZHIP -/- line. The red lambda indicates the epitope recognized by antibody detecting EZHIP protein. Western blot analysis (right panel) of cell lines expressing EZHIP FL or mutants probed with antibodies indicated on the left. (D) Mean-Difference plot showing average log2 counts per million (logCPM) versus log2 fold-change (logFC) expression between U2OS WT and EED -/- or U2OS WT and EZHIP -/- (right). Right corner: number of genes significantly differentially expressed (FDR<0.05) corresponding to the dots colored in red. n=2.

    Article Snippet: Polyclonal Rabbit one against H3 from Cell Signaling Technology (9715 ) ; H3K9me2 (ab1220) and H3K27Ac (ab 4729) were purchased from Abcam; H3K27me1 mouse mAb C0321 from Active Motif; H3K27me3 Rabbit mAb C36B11 (9733), H3K4me3 Rabbit mAb C42D8 (9751), Rabbit mAb D7C6X (14129), Rabbit mAb H2AK119ub (8240S) and mouse mAb10E2 HDAC1 (5356S) from Ozyme (Cell Signaling Technology). hEZHIP (HPA006128) and mAb Flag-M2 (F1804) purchased from SIGMA; Anti-Germ cell-specific Rabbit Polyclonal DPP3A/Stella (19878) and TRA98 (Ab82527) Rat monoclonal one from Abcam.

    Techniques: Western Blot, Methylation, Stable Transfection, Expressing

    Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and Hdac1, in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.

    Journal: Disease Models & Mechanisms

    Article Title: Identification of early indicators of altered metabolism in normal development using a rodent model system

    doi: 10.1242/dmm.031815

    Figure Lengend Snippet: Regulation of the cell cycle is different in ABW and LBW pups. (A) Western blot analyses of the skeletal muscle of members of the Dnmt1 repressor complex, E2f1, Rb and Hdac1, in ABW and LBW pups at day 1. Gapdh was used as a loading control. (B) Co-IP analysis of the Dnmt1 repressor complex using anti-Dnmt1 antibody, and western blot analysis of the members of the complex E2f1, Rb, Hdac1, in ABW and LBW pups at day 1. Co-IP using IgG was used as a control; 5% of the total extract was used as input. (C) Western blot analysis of skeletal muscle of 1-day-old ABW and LBW pups for phospho Rb (ser 608, ser 780 and ser 795). Gapdh was used as a loading control. (D) Western blot analysis of cyclin D1 and cyclin-dependent kinases Cdk4 and Cdk6, and the G1 phase cell cycle inhibitors P16 and P21, using skeletal muscle of ABW and LBW pups at day 1. Gapdh was used as a loading control.

    Article Snippet: Antibodies used were as follows: anti-Dnmt1 (ab92453), anti-Cdk4 (ab131197), anti-Hdac1 (ab7028), anti-cyclin D1 (134175), anti-Glut4 (ab33780), anti-ph-Akt (ab81283), anti-Dnmt3a (ab13888), anti-Dnmt3b ab13604), anti-Gcg (ab92517), anti-ph-IRS1 (ab1194), anti-Akt1/2/3 (ab126811), anti-Pparγ (ab45036) and anti-Rb (ab6075), purchased from Abcam, USA; anti-Pparγ (sc7273) and anti-Rxrα (sc 553), purchased from Santa Cruz Biotechnology, USA; anti-ph-Rb ser780 (RA2133264) and anti-ph-Rb ser 795 (QL2133271), from Thermo Fisher Scientific; anti-Gapdh (2118L), anti-ph-Rb ser 608 (2181s), anti-ph-Akt (4060L), anti-Irs-1 (3407S), anti-Hdac1 (5356s) and anti-P21 (2947s), from Cell Signaling Technology, USA; anti-E2f1 (2651877), from Millipore, USA; anti-AdipoQ (A6354), from Sigma-Aldrich; anti-P16 (10883-1-AP), from Protein Tech, USA; and anti-insulin, from Dako Cytomation, Copenhagen, Denmark.

    Techniques: Western Blot, Co-Immunoprecipitation Assay

    Possible mechanism by which embryonic growth is coordinated and correlated with the maternal environment. Elevated Dnmt1 expression might be increasing methylation in the promoters of genes, resulting in suppression of the transcription of genes required for normal growth. In addition, the formation of the Dnmt1 repressive complex with Rb, E2f1 and Hdac1 might slow down cell cycle progression. These processes, probably working together, could be responsible for the growth reduction observed in the birth weight of LBW pups.

    Journal: Disease Models & Mechanisms

    Article Title: Identification of early indicators of altered metabolism in normal development using a rodent model system

    doi: 10.1242/dmm.031815

    Figure Lengend Snippet: Possible mechanism by which embryonic growth is coordinated and correlated with the maternal environment. Elevated Dnmt1 expression might be increasing methylation in the promoters of genes, resulting in suppression of the transcription of genes required for normal growth. In addition, the formation of the Dnmt1 repressive complex with Rb, E2f1 and Hdac1 might slow down cell cycle progression. These processes, probably working together, could be responsible for the growth reduction observed in the birth weight of LBW pups.

    Article Snippet: Antibodies used were as follows: anti-Dnmt1 (ab92453), anti-Cdk4 (ab131197), anti-Hdac1 (ab7028), anti-cyclin D1 (134175), anti-Glut4 (ab33780), anti-ph-Akt (ab81283), anti-Dnmt3a (ab13888), anti-Dnmt3b ab13604), anti-Gcg (ab92517), anti-ph-IRS1 (ab1194), anti-Akt1/2/3 (ab126811), anti-Pparγ (ab45036) and anti-Rb (ab6075), purchased from Abcam, USA; anti-Pparγ (sc7273) and anti-Rxrα (sc 553), purchased from Santa Cruz Biotechnology, USA; anti-ph-Rb ser780 (RA2133264) and anti-ph-Rb ser 795 (QL2133271), from Thermo Fisher Scientific; anti-Gapdh (2118L), anti-ph-Rb ser 608 (2181s), anti-ph-Akt (4060L), anti-Irs-1 (3407S), anti-Hdac1 (5356s) and anti-P21 (2947s), from Cell Signaling Technology, USA; anti-E2f1 (2651877), from Millipore, USA; anti-AdipoQ (A6354), from Sigma-Aldrich; anti-P16 (10883-1-AP), from Protein Tech, USA; and anti-insulin, from Dako Cytomation, Copenhagen, Denmark.

    Techniques: Expressing, Methylation