anti 5hmc antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti 5hmc antibody
    Anti 5hmc Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti 5hmc antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    anti 5hmc antibody - by Bioz Stars, 2023-01
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    anti 5hmc antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti 5hmc antibody
    Anti 5hmc Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti 5hmc antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    anti 5hmc antibody - by Bioz Stars, 2023-01
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    anti 5hmc antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti 5hmc antibody
    a , Western blot analysis of GSDME expression in PANC-1, AsPC-1, BxPC-3, HL60, K562, BT549, MDA-MB-231, OPM2 and RPMI-8266 cells. b , ChIP–qPCR analysis performed with <t>anti-5hMC</t> and primers specific for GSDME in PANC-1, AsPC-1 and BxPC-3 cells. c , d , BxPC-3 cells transfected with SGCTR or GSDME -SGs (2 × 10 6 cells) were subcutaneously injected into mice. Tumours were sized ( c , n = 6), photographed and weighed ( d , n = 5). e , f , BxPC-3 cells transfected with SGCTR, GSDME -SGs or GSDME -SG/Flag- GSDME (2.5 × 10 5 cells) were orthotopically injected into the pancreas of mice. Tumours were photographed (left) and weighed (right) ( n = 6). Normal pancreas served as the control. Scale bars, 1 cm. In a and b , n = 3 biological independent experiments. P values were determined by one-way ANOVA Bonferroni’s test ( b – f ). The data represent the mean ± s.d.
    Anti 5hmc Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti 5hmc antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti 5hmc antibody - by Bioz Stars, 2023-01
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    1) Product Images from "Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1–mucin pathway"

    Article Title: Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1–mucin pathway

    Journal: Nature Cell Biology

    doi: 10.1038/s41556-022-00857-4

    a , Western blot analysis of GSDME expression in PANC-1, AsPC-1, BxPC-3, HL60, K562, BT549, MDA-MB-231, OPM2 and RPMI-8266 cells. b , ChIP–qPCR analysis performed with anti-5hMC and primers specific for GSDME in PANC-1, AsPC-1 and BxPC-3 cells. c , d , BxPC-3 cells transfected with SGCTR or GSDME -SGs (2 × 10 6 cells) were subcutaneously injected into mice. Tumours were sized ( c , n = 6), photographed and weighed ( d , n = 5). e , f , BxPC-3 cells transfected with SGCTR, GSDME -SGs or GSDME -SG/Flag- GSDME (2.5 × 10 5 cells) were orthotopically injected into the pancreas of mice. Tumours were photographed (left) and weighed (right) ( n = 6). Normal pancreas served as the control. Scale bars, 1 cm. In a and b , n = 3 biological independent experiments. P values were determined by one-way ANOVA Bonferroni’s test ( b – f ). The data represent the mean ± s.d.
    Figure Legend Snippet: a , Western blot analysis of GSDME expression in PANC-1, AsPC-1, BxPC-3, HL60, K562, BT549, MDA-MB-231, OPM2 and RPMI-8266 cells. b , ChIP–qPCR analysis performed with anti-5hMC and primers specific for GSDME in PANC-1, AsPC-1 and BxPC-3 cells. c , d , BxPC-3 cells transfected with SGCTR or GSDME -SGs (2 × 10 6 cells) were subcutaneously injected into mice. Tumours were sized ( c , n = 6), photographed and weighed ( d , n = 5). e , f , BxPC-3 cells transfected with SGCTR, GSDME -SGs or GSDME -SG/Flag- GSDME (2.5 × 10 5 cells) were orthotopically injected into the pancreas of mice. Tumours were photographed (left) and weighed (right) ( n = 6). Normal pancreas served as the control. Scale bars, 1 cm. In a and b , n = 3 biological independent experiments. P values were determined by one-way ANOVA Bonferroni’s test ( b – f ). The data represent the mean ± s.d.

    Techniques Used: Western Blot, Expressing, Transfection, Injection

    anti 5 hydoxymethyl cytosine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti 5 hydoxymethyl cytosine
    Anti 5 Hydoxymethyl Cytosine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti 5 hydoxymethyl cytosine/product/Cell Signaling Technology Inc
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    anti 5 hydoxymethyl cytosine - by Bioz Stars, 2023-01
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    mouse monoclonal anti 5 hydroxymethylcytosine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal anti 5 hydroxymethylcytosine
    KEY RESOURCE TABLE
    Mouse Monoclonal Anti 5 Hydroxymethylcytosine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti 5 hydroxymethylcytosine/product/Cell Signaling Technology Inc
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    1) Product Images from "MYC regulation of D2HGDH and L2HGDH influences the epigenome and epitranscriptome"

    Article Title: MYC regulation of D2HGDH and L2HGDH influences the epigenome and epitranscriptome

    Journal: Cell chemical biology

    doi: 10.1016/j.chembiol.2020.02.002

    KEY RESOURCE TABLE
    Figure Legend Snippet: KEY RESOURCE TABLE

    Techniques Used: Recombinant, Western Blot, Stripping, Activity Assay, Methylation, DNA Purification, Luciferase, Enzymatic Assay, Cell Isolation, shRNA, Plasmid Preparation, Software

    mouse monoclonal anti 5 hydroxymethylcytosine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal anti 5 hydroxymethylcytosine
    KEY RESOURCES TABLE
    Mouse Monoclonal Anti 5 Hydroxymethylcytosine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti 5 hydroxymethylcytosine/product/Cell Signaling Technology Inc
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    mouse monoclonal anti 5 hydroxymethylcytosine - by Bioz Stars, 2023-01
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    1) Product Images from "Maternal Inactivity Programs Skeletal Muscle Dysfunction in Offspring Mice by Attenuating Apelin Signaling and Mitochondrial Biogenesis"

    Article Title: Maternal Inactivity Programs Skeletal Muscle Dysfunction in Offspring Mice by Attenuating Apelin Signaling and Mitochondrial Biogenesis

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.108461

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Software, Imaging

    5 hmc mouse monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 5 hmc mouse monoclonal antibody
    5 Hmc Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ma 51660  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ma 51660
    Ma 51660, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ma 51660/product/Cell Signaling Technology Inc
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    5 hydroxymethylcytosine antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 5 hydroxymethylcytosine antibody
    Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) <t>5hmC</t> and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.
    5 Hydroxymethylcytosine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 hydroxymethylcytosine antibody/product/Cell Signaling Technology Inc
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    5 hydroxymethylcytosine antibody - by Bioz Stars, 2023-01
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    1) Product Images from "Butyrate inhibits indices of colorectal carcinogenesis via enhancing α-ketoglutarate-dependent DNA demethylation of mismatch repair genes"

    Article Title: Butyrate inhibits indices of colorectal carcinogenesis via enhancing α-ketoglutarate-dependent DNA demethylation of mismatch repair genes

    Journal: Molecular nutrition & food research

    doi: 10.1002/mnfr.201700932

    Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) 5hmC and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.
    Figure Legend Snippet: Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) 5hmC and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.

    Techniques Used: Transfection, Plasmid Preparation, Translocation Assay

    Cells were treated with 0 (□) or 4 mM sodium butyrate (NaB, ■) for 4 d. (A) Protein contents of acetylated histone H3 (Ac-H3), acetylated histone H4 (Ac-H4) and Ten-eleven translocation 3 (TET3). (B) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). 5hmC and 5mC modifications in the promoter of MSH2 (C) and MLH1 (D). Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.
    Figure Legend Snippet: Cells were treated with 0 (□) or 4 mM sodium butyrate (NaB, ■) for 4 d. (A) Protein contents of acetylated histone H3 (Ac-H3), acetylated histone H4 (Ac-H4) and Ten-eleven translocation 3 (TET3). (B) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). 5hmC and 5mC modifications in the promoter of MSH2 (C) and MLH1 (D). Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.

    Techniques Used: Translocation Assay

    anti myc mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti myc mab
    Anti Myc Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti 5hmc antibody
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    Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) <t>5hmC</t> and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.
    5 Hydroxymethylcytosine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) <t>5hmC</t> and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.
    Anti Myc Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    KEY RESOURCE TABLE

    Journal: Cell chemical biology

    Article Title: MYC regulation of D2HGDH and L2HGDH influences the epigenome and epitranscriptome

    doi: 10.1016/j.chembiol.2020.02.002

    Figure Lengend Snippet: KEY RESOURCE TABLE

    Article Snippet: Mouse monoclonal anti-5-hydroxymethylcytosine (Clone HMC31) , Cell Signaling Technology , Cat#51660, RRID:AB_2799398.

    Techniques: Recombinant, Western Blot, Stripping, Activity Assay, Methylation, DNA Purification, Luciferase, Enzymatic Assay, Cell Isolation, shRNA, Plasmid Preparation, Software

    Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) 5hmC and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.

    Journal: Molecular nutrition & food research

    Article Title: Butyrate inhibits indices of colorectal carcinogenesis via enhancing α-ketoglutarate-dependent DNA demethylation of mismatch repair genes

    doi: 10.1002/mnfr.201700932

    Figure Lengend Snippet: Caco-2 cells were transfected with isocitrate dehydrogenase 1 (IDH1) knock-down (KD) plasmid or IDH1 wild-type (WT) plasmid to downregulate (−) or over-express (+) IDH1. IDH1 KD cells then were treated with 0 (−) or 4 mM sodium butyrate (NaB, +), or 4 mM NaB supplemented with 0 (−) or 4 mM dimethyl-α-ketoglutarate (a membrane permeable α-ketoglutarate, α-KG, +). (A) Protein contents of IDH1 and cleaved PARP. (B) Protein contents of ten-eleven translocation 3 (TET3). (C) Protein contents of acetylated histone H3 (Ac-H3) and acetylated histone H4 (Ac-H4). (D) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). (E) 5hmC and (F) 5mC modifications in the promoter of MSH2 and MLH1. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.

    Article Snippet: Then, 1/50 5-hydroxymethylcytosine antibody (5hmC, Cell Signaling Technology), 5-methylcytosine antibody (5mC, Cell Signaling Technology), or normal rabbit IgG (Cell Signaling Technology) was added to denatured DNA.

    Techniques: Transfection, Plasmid Preparation, Translocation Assay

    Cells were treated with 0 (□) or 4 mM sodium butyrate (NaB, ■) for 4 d. (A) Protein contents of acetylated histone H3 (Ac-H3), acetylated histone H4 (Ac-H4) and Ten-eleven translocation 3 (TET3). (B) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). 5hmC and 5mC modifications in the promoter of MSH2 (C) and MLH1 (D). Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.

    Journal: Molecular nutrition & food research

    Article Title: Butyrate inhibits indices of colorectal carcinogenesis via enhancing α-ketoglutarate-dependent DNA demethylation of mismatch repair genes

    doi: 10.1002/mnfr.201700932

    Figure Lengend Snippet: Cells were treated with 0 (□) or 4 mM sodium butyrate (NaB, ■) for 4 d. (A) Protein contents of acetylated histone H3 (Ac-H3), acetylated histone H4 (Ac-H4) and Ten-eleven translocation 3 (TET3). (B) Protein contents of MutS homolog 2 (MSH2) and MutL homolog 1 (MLH1). 5hmC and 5mC modifications in the promoter of MSH2 (C) and MLH1 (D). Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.

    Article Snippet: Then, 1/50 5-hydroxymethylcytosine antibody (5hmC, Cell Signaling Technology), 5-methylcytosine antibody (5mC, Cell Signaling Technology), or normal rabbit IgG (Cell Signaling Technology) was added to denatured DNA.

    Techniques: Translocation Assay