rabbit monoclonal anti frizzled 6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti frizzled 6
    Western blot protein expression analysis of FZD4 (60 kDa), <t>FZD6</t> (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.
    Rabbit Monoclonal Anti Frizzled 6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti frizzled 6/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti frizzled 6 - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells"

    Article Title: Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2017/8379256

    Western blot protein expression analysis of FZD4 (60 kDa), FZD6 (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.
    Figure Legend Snippet: Western blot protein expression analysis of FZD4 (60 kDa), FZD6 (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.

    Techniques Used: Western Blot, Expressing, Incubation, Activity Assay

    rabbit monoclonal anti frizzled 6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti frizzled 6
    Western blot protein expression analysis of FZD4 (60 kDa), <t>FZD6</t> (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.
    Rabbit Monoclonal Anti Frizzled 6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti frizzled 6/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti frizzled 6 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells"

    Article Title: Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

    Journal: Mediators of Inflammation

    doi: 10.1155/2017/8379256

    Western blot protein expression analysis of FZD4 (60 kDa), FZD6 (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.
    Figure Legend Snippet: Western blot protein expression analysis of FZD4 (60 kDa), FZD6 (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.

    Techniques Used: Western Blot, Expressing, Incubation, Activity Assay

    rabbit anti tgfβ r2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti tgfβ r2
    Effects of PNS on gene expression in the hippocampus: impact on TGF-β1 pathway and oxidative stress machinery. Effects induced by PNS exposure on (A,B) TGF-β1, (C,D) <t>TGFβ-R2,</t> (E,F) iNOS, (G,H) NOX1, and (I,J) NOX2 mRNAs expression measured by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH/β-actin (HSKP) mRNA, as endogenous controls. As a negative control, a reaction in absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed at least in triplicate. Data are shown as mean ± S.E.M. * p < 0.05.
    Rabbit Anti Tgfβ R2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tgfβ r2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti tgfβ r2 - by Bioz Stars, 2023-02
    86/100 stars

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    1) Product Images from "Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway"

    Article Title: Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2022.1075746

    Effects of PNS on gene expression in the hippocampus: impact on TGF-β1 pathway and oxidative stress machinery. Effects induced by PNS exposure on (A,B) TGF-β1, (C,D) TGFβ-R2, (E,F) iNOS, (G,H) NOX1, and (I,J) NOX2 mRNAs expression measured by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH/β-actin (HSKP) mRNA, as endogenous controls. As a negative control, a reaction in absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed at least in triplicate. Data are shown as mean ± S.E.M. * p < 0.05.
    Figure Legend Snippet: Effects of PNS on gene expression in the hippocampus: impact on TGF-β1 pathway and oxidative stress machinery. Effects induced by PNS exposure on (A,B) TGF-β1, (C,D) TGFβ-R2, (E,F) iNOS, (G,H) NOX1, and (I,J) NOX2 mRNAs expression measured by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH/β-actin (HSKP) mRNA, as endogenous controls. As a negative control, a reaction in absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed at least in triplicate. Data are shown as mean ± S.E.M. * p < 0.05.

    Techniques Used: Expressing, Quantitative RT-PCR, Negative Control

    Role of TGF-β1 pathway in the mechanisms of vulnerability and resilience to PNS exposure. Effects induced by PNS exposure on TGF-β1 levels and of its receptor TGFβ-R2 in total protein extracts from hippocampus of CTRL and PNS rats evaluated by Western Blot analysis. (A) Representative immunoblot and histogram of TGF-β1 (44 kDa) in CTRL and PNS male adolescent rats (* p < 0.05 vs. PNS V). (B) Representative immunoblot and histogram of TGF-β1 (44 kDa) in CTRL and PNS female adolescent rats (* p < 0.05 vs. CTRL; * p < 0.05 vs. PNS V). (C) Representative immunoblot and histogram of TGFβ-R2 (65 kDa) in CTRL and PNS male adolescent rats (** p < 0.01 vs. CTRL; *** p < 0.001 vs. PNS V). (D) Representative immunoblot and histogram of TGFβ-R2 (65 kDa) in CTRL and PNS female adolescent rats (* p < 0.05 vs. CTRL; * p < 0.05 vs. PNS V). TGF-β1 and TGFβ-R2 densitometric values were normalized against actin used as internal control. Plasmatic levels of TGF-β1 (ng/ml) in male (E) (** p < 0.01 vs. CTRL) and female (F) . All data are shown as mean ± SEM of CTRL male n = 10; PNS V male n = 9; PNS R male n = 5; CTRL female n = 10; PNS V female n = 5; PNS R female n = 4.
    Figure Legend Snippet: Role of TGF-β1 pathway in the mechanisms of vulnerability and resilience to PNS exposure. Effects induced by PNS exposure on TGF-β1 levels and of its receptor TGFβ-R2 in total protein extracts from hippocampus of CTRL and PNS rats evaluated by Western Blot analysis. (A) Representative immunoblot and histogram of TGF-β1 (44 kDa) in CTRL and PNS male adolescent rats (* p < 0.05 vs. PNS V). (B) Representative immunoblot and histogram of TGF-β1 (44 kDa) in CTRL and PNS female adolescent rats (* p < 0.05 vs. CTRL; * p < 0.05 vs. PNS V). (C) Representative immunoblot and histogram of TGFβ-R2 (65 kDa) in CTRL and PNS male adolescent rats (** p < 0.01 vs. CTRL; *** p < 0.001 vs. PNS V). (D) Representative immunoblot and histogram of TGFβ-R2 (65 kDa) in CTRL and PNS female adolescent rats (* p < 0.05 vs. CTRL; * p < 0.05 vs. PNS V). TGF-β1 and TGFβ-R2 densitometric values were normalized against actin used as internal control. Plasmatic levels of TGF-β1 (ng/ml) in male (E) (** p < 0.01 vs. CTRL) and female (F) . All data are shown as mean ± SEM of CTRL male n = 10; PNS V male n = 9; PNS R male n = 5; CTRL female n = 10; PNS V female n = 5; PNS R female n = 4.

    Techniques Used: Western Blot

    anti phospholacetyllcoa carboxylase  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospholacetyllcoa carboxylase
    Anti Phospholacetyllcoa Carboxylase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospholacetyllcoa carboxylase/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti phospholacetyllcoa carboxylase - by Bioz Stars, 2023-02
    99/100 stars

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    anti p acc  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p acc
    Anti P Acc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p acc/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti p acc - by Bioz Stars, 2023-02
    86/100 stars

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    phospho acetyl coa carboxylase ser79 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho acetyl coa carboxylase ser79 antibody
    Phospho Acetyl Coa Carboxylase Ser79 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho acetyl coa carboxylase ser79 antibody/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    phospho acetyl coa carboxylase ser79 antibody - by Bioz Stars, 2023-02
    99/100 stars

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    anti β catenin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti β catenin
    Anti β Catenin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti β catenin/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti β catenin - by Bioz Stars, 2023-02
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    rabbit anti phospho acetyl coa carboxylase ser79  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti phospho acetyl coa carboxylase ser79
    Rabbit Anti Phospho Acetyl Coa Carboxylase Ser79, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho acetyl coa carboxylase ser79/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
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    rabbit anti phospho acetyl coa carboxylase ser79 - by Bioz Stars, 2023-02
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    di methylated h3k4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc di methylated h3k4
    (A‐C), Immunohistochemistry staining for lysine‐specific demethylase 1 (LSD1) in mice testes from vehicle control (A), busulfan (B), and NCL1 3.0 mg/kg (C) groups. (D‐H), Terminal deoxy nucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining for apoptosis in mice testes from vehicle control (D), busulfan (E), NCL1 1.0 mg/kg (F), and NCL1 3.0 mg/kg (G) groups. Arrowheads indicate TUNEL‐positive cells. Scale bars indicate 100 µm. (H), Percentage of seminiferous tubules presenting with TUNEL‐positive cells. Mean ± standard deviation (SD); ** P < .01 compared to control group. (I), Western blot analyses following vehicle control, busulfan, or NCL1 3.0 mg/kg treatment using anti‐LSD1, anti‐androgen receptor (AR), anti‐connexin 43 (Cx43), anti‐histone <t>H3</t> <t>lysine</t> <t>4</t> mono/dimethyl (H3K4me1/me2), and anti‐cleaved caspase (CCas) 3/7/8/9 antibodies. (n = 3 in each group). (J‐M), Immunohistochemistry staining for Cx43 in mice testes from vehicle control (J) and NCL1 3.0 mg/kg (K) groups. Arrowheads indicate Leydig cell nodules. Scale bars indicate 40 µm. (L), Area of Leydig cell nodules. (M), Relative intensity of Cx43. Mean ± standard deviation (SD); ** P < .01, statistically significant. NCL1 (N‐[(1S)‐3‐[3‐(trans‐2‐aminocyclopropyl)phenoxy]‐1‐(benzylcarbamoyl)propyl] benzamide
    Di Methylated H3k4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/di methylated h3k4/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    di methylated h3k4 - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "Selective lysine‐specific demethylase 1 inhibitor, NCL1, could cause testicular toxicity via the regulation of apoptosis"

    Article Title: Selective lysine‐specific demethylase 1 inhibitor, NCL1, could cause testicular toxicity via the regulation of apoptosis

    Journal: Andrology

    doi: 10.1111/andr.12846

    (A‐C), Immunohistochemistry staining for lysine‐specific demethylase 1 (LSD1) in mice testes from vehicle control (A), busulfan (B), and NCL1 3.0 mg/kg (C) groups. (D‐H), Terminal deoxy nucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining for apoptosis in mice testes from vehicle control (D), busulfan (E), NCL1 1.0 mg/kg (F), and NCL1 3.0 mg/kg (G) groups. Arrowheads indicate TUNEL‐positive cells. Scale bars indicate 100 µm. (H), Percentage of seminiferous tubules presenting with TUNEL‐positive cells. Mean ± standard deviation (SD); ** P < .01 compared to control group. (I), Western blot analyses following vehicle control, busulfan, or NCL1 3.0 mg/kg treatment using anti‐LSD1, anti‐androgen receptor (AR), anti‐connexin 43 (Cx43), anti‐histone H3 lysine 4 mono/dimethyl (H3K4me1/me2), and anti‐cleaved caspase (CCas) 3/7/8/9 antibodies. (n = 3 in each group). (J‐M), Immunohistochemistry staining for Cx43 in mice testes from vehicle control (J) and NCL1 3.0 mg/kg (K) groups. Arrowheads indicate Leydig cell nodules. Scale bars indicate 40 µm. (L), Area of Leydig cell nodules. (M), Relative intensity of Cx43. Mean ± standard deviation (SD); ** P < .01, statistically significant. NCL1 (N‐[(1S)‐3‐[3‐(trans‐2‐aminocyclopropyl)phenoxy]‐1‐(benzylcarbamoyl)propyl] benzamide
    Figure Legend Snippet: (A‐C), Immunohistochemistry staining for lysine‐specific demethylase 1 (LSD1) in mice testes from vehicle control (A), busulfan (B), and NCL1 3.0 mg/kg (C) groups. (D‐H), Terminal deoxy nucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining for apoptosis in mice testes from vehicle control (D), busulfan (E), NCL1 1.0 mg/kg (F), and NCL1 3.0 mg/kg (G) groups. Arrowheads indicate TUNEL‐positive cells. Scale bars indicate 100 µm. (H), Percentage of seminiferous tubules presenting with TUNEL‐positive cells. Mean ± standard deviation (SD); ** P < .01 compared to control group. (I), Western blot analyses following vehicle control, busulfan, or NCL1 3.0 mg/kg treatment using anti‐LSD1, anti‐androgen receptor (AR), anti‐connexin 43 (Cx43), anti‐histone H3 lysine 4 mono/dimethyl (H3K4me1/me2), and anti‐cleaved caspase (CCas) 3/7/8/9 antibodies. (n = 3 in each group). (J‐M), Immunohistochemistry staining for Cx43 in mice testes from vehicle control (J) and NCL1 3.0 mg/kg (K) groups. Arrowheads indicate Leydig cell nodules. Scale bars indicate 40 µm. (L), Area of Leydig cell nodules. (M), Relative intensity of Cx43. Mean ± standard deviation (SD); ** P < .01, statistically significant. NCL1 (N‐[(1S)‐3‐[3‐(trans‐2‐aminocyclopropyl)phenoxy]‐1‐(benzylcarbamoyl)propyl] benzamide

    Techniques Used: Immunohistochemistry, Staining, End Labeling, TUNEL Assay, Standard Deviation, Western Blot

    pacc ser 79  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pacc ser 79
    Pacc Ser 79, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacc ser 79/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    pacc ser 79 - by Bioz Stars, 2023-02
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    anti phospho acc  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho acc
    Anti Phospho Acc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho acc/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti phospho acc - by Bioz Stars, 2023-02
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