glutaminase 1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc glutaminase 1
    Cellular effects on differences of substrate preference on dTMP. ( A ) Intracellular dNTP pools with 5′ nucleotidases with different substrate preferences. CdN: cytosolic 5′-nucleotidase; NT5M: human mitochondrial 5′-nucleotidase (signal-sequence deleted). ( B ) Cell viability assay. Cells were plated 24 h in a 6-well plate at 5 × 10 5 cells/well, then transfected with 1.25 µg of mRNA for 24 h. For quantification, the amount of formazan dye formed was measured at 450 nm. ( C ) ECAR and OCR assay for comparing 5′-nucleotidase substrate preferences in MDA-MB-231. ( D ) Western blot analysis for representative proteins related to cellular function. Hexokinase (HK1/2) in glycolysis; α-ketoglutarate dehydrogenase (OGDH) in important branching position of TCA cycle; glutamate dehydrogenase (GLUD) and glutaminase <t>(GLS1)</t> in glutaminolysis; thymidylate synthase (TYMS) and thymidine kinase (TK1) in essential entry position of dTTP synthesis; cleaved poly ADP-ribose polymerase (PARP) and phosphorylated H2AX (γH2AX) as apoptosis marker. Original blots are presented in (Figure Raw data).
    Glutaminase 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutaminase 1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glutaminase 1 - by Bioz Stars, 2023-02
    86/100 stars

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    1) Product Images from "dTMP imbalance through thymidylate 5′-phosphohydrolase activity induces apoptosis in triple-negative breast cancers"

    Article Title: dTMP imbalance through thymidylate 5′-phosphohydrolase activity induces apoptosis in triple-negative breast cancers

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-24706-4

    Cellular effects on differences of substrate preference on dTMP. ( A ) Intracellular dNTP pools with 5′ nucleotidases with different substrate preferences. CdN: cytosolic 5′-nucleotidase; NT5M: human mitochondrial 5′-nucleotidase (signal-sequence deleted). ( B ) Cell viability assay. Cells were plated 24 h in a 6-well plate at 5 × 10 5 cells/well, then transfected with 1.25 µg of mRNA for 24 h. For quantification, the amount of formazan dye formed was measured at 450 nm. ( C ) ECAR and OCR assay for comparing 5′-nucleotidase substrate preferences in MDA-MB-231. ( D ) Western blot analysis for representative proteins related to cellular function. Hexokinase (HK1/2) in glycolysis; α-ketoglutarate dehydrogenase (OGDH) in important branching position of TCA cycle; glutamate dehydrogenase (GLUD) and glutaminase (GLS1) in glutaminolysis; thymidylate synthase (TYMS) and thymidine kinase (TK1) in essential entry position of dTTP synthesis; cleaved poly ADP-ribose polymerase (PARP) and phosphorylated H2AX (γH2AX) as apoptosis marker. Original blots are presented in (Figure Raw data).
    Figure Legend Snippet: Cellular effects on differences of substrate preference on dTMP. ( A ) Intracellular dNTP pools with 5′ nucleotidases with different substrate preferences. CdN: cytosolic 5′-nucleotidase; NT5M: human mitochondrial 5′-nucleotidase (signal-sequence deleted). ( B ) Cell viability assay. Cells were plated 24 h in a 6-well plate at 5 × 10 5 cells/well, then transfected with 1.25 µg of mRNA for 24 h. For quantification, the amount of formazan dye formed was measured at 450 nm. ( C ) ECAR and OCR assay for comparing 5′-nucleotidase substrate preferences in MDA-MB-231. ( D ) Western blot analysis for representative proteins related to cellular function. Hexokinase (HK1/2) in glycolysis; α-ketoglutarate dehydrogenase (OGDH) in important branching position of TCA cycle; glutamate dehydrogenase (GLUD) and glutaminase (GLS1) in glutaminolysis; thymidylate synthase (TYMS) and thymidine kinase (TK1) in essential entry position of dTTP synthesis; cleaved poly ADP-ribose polymerase (PARP) and phosphorylated H2AX (γH2AX) as apoptosis marker. Original blots are presented in (Figure Raw data).

    Techniques Used: Sequencing, Viability Assay, Transfection, Western Blot, Cell Function Assay, Marker

    glutaminase 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 86

    Structured Review

    Cell Signaling Technology Inc glutaminase 1
    Glutaminase 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutaminase 1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glutaminase 1 - by Bioz Stars, 2023-02
    86/100 stars

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