cell viability dye 7 aad  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell viability dye 7 aad
    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.
    Cell Viability Dye 7 Aad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell viability dye 7 aad/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell viability dye 7 aad - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "T Cells from Programmed Death-1 Deficient Mice Respond Poorly to Mycobacterium tuberculosis Infection"

    Article Title: T Cells from Programmed Death-1 Deficient Mice Respond Poorly to Mycobacterium tuberculosis Infection

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0019864

    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.
    Figure Legend Snippet: Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.

    Techniques Used: Isolation, Staining, Marker, Flow Cytometry, Expressing

    cell viability dye 7 aad  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 94

    Structured Review

    Cell Signaling Technology Inc cell viability dye 7 aad
    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.
    Cell Viability Dye 7 Aad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell viability dye 7 aad/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell viability dye 7 aad - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "T Cells from Programmed Death-1 Deficient Mice Respond Poorly to Mycobacterium tuberculosis Infection"

    Article Title: T Cells from Programmed Death-1 Deficient Mice Respond Poorly to Mycobacterium tuberculosis Infection

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0019864

    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.
    Figure Legend Snippet: Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.

    Techniques Used: Isolation, Staining, Marker, Flow Cytometry, Expressing

    annexin v  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc annexin v
    Annexin V, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v - by Bioz Stars, 2023-02
    94/100 stars

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    7aad staining  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 7aad staining
    Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin <t>V/7AAD</t> analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).
    7aad Staining, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7aad staining/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7aad staining - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Oxidative Stress-Dependent Synergistic Antiproliferation, Apoptosis, and DNA Damage of Ultraviolet-C and Coral-Derived Sinularin Combined Treatment for Oral Cancer Cells"

    Article Title: Oxidative Stress-Dependent Synergistic Antiproliferation, Apoptosis, and DNA Damage of Ultraviolet-C and Coral-Derived Sinularin Combined Treatment for Oral Cancer Cells

    Journal: Cancers

    doi: 10.3390/cancers13102450

    Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin V/7AAD analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).
    Figure Legend Snippet: Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin V/7AAD analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).

    Techniques Used:

    7 aminoactinomycin d  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 7 aminoactinomycin d
    7 Aminoactinomycin D, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7 aminoactinomycin d/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    7 aminoactinomycin d - by Bioz Stars, 2023-02
    94/100 stars

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    live cell gsh staining solution  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc live cell gsh staining solution
    Live Cell Gsh Staining Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/live cell gsh staining solution/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    live cell gsh staining solution - by Bioz Stars, 2023-02
    94/100 stars

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    Cell Signaling Technology Inc cell viability dye 7 aad
    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.
    Cell Viability Dye 7 Aad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell viability dye 7 aad/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell viability dye 7 aad - by Bioz Stars, 2023-02
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    94
    Cell Signaling Technology Inc annexin v
    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.
    Annexin V, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    annexin v - by Bioz Stars, 2023-02
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    Cell Signaling Technology Inc 7aad staining
    Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin <t>V/7AAD</t> analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).
    7aad Staining, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7aad staining/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7aad staining - by Bioz Stars, 2023-02
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    Cell Signaling Technology Inc 7 aminoactinomycin d
    Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin <t>V/7AAD</t> analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).
    7 Aminoactinomycin D, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7 aminoactinomycin d/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    7 aminoactinomycin d - by Bioz Stars, 2023-02
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    Cell Signaling Technology Inc live cell gsh staining solution
    Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin <t>V/7AAD</t> analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).
    Live Cell Gsh Staining Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/live cell gsh staining solution/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    live cell gsh staining solution - by Bioz Stars, 2023-02
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    Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.

    Journal: PLoS ONE

    Article Title: T Cells from Programmed Death-1 Deficient Mice Respond Poorly to Mycobacterium tuberculosis Infection

    doi: 10.1371/journal.pone.0019864

    Figure Lengend Snippet: Macrophages were isolated from the peritoneum of WT and PD-1 −/− mice. Peritoneal macrophages were stained with surface markers anti-CD11b and anti-CD11c and surface stained cells were intracellularly stained for the LC3-B autophagy marker. Cell viability dye 7-AAD was added before acquisition of cells by flow cytometry. Peritoneal macrophage cells were gated for CD11b and CD11c to analyze the expression of LC3-B and 7-AAD. Data shown here are representative of two experiments.

    Article Snippet: LC3-B staining was performed according to the manufacturer's protocol (Cell Signalling, USA) and cell viability dye (7-AAD) was added to the LC3-B stained cells 15 minutes before analyzing the cells by flow cytometry.

    Techniques: Isolation, Staining, Marker, Flow Cytometry, Expressing

    Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin V/7AAD analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).

    Journal: Cancers

    Article Title: Oxidative Stress-Dependent Synergistic Antiproliferation, Apoptosis, and DNA Damage of Ultraviolet-C and Coral-Derived Sinularin Combined Treatment for Oral Cancer Cells

    doi: 10.3390/cancers13102450

    Figure Lengend Snippet: Annexin V-detected apoptosis assays of UVC and/or sinularin treatments. Following the NAC preincubation (5 mM for 1 h) or not, the cells were arranged into four kinds of treatments: the control (0.1% DMSO in medium), UVC, sinularin, and UVC/sinularin for 48 h. The UVC and/or sinularin conditions were 12 J/m 2 , 2 μM and 10 J/m 2 , 3 μM for oral cancer cells (Ca9-22, CAL 27), and 12 J/m 2 , 3 μM for normal cells (HGF-1) for 48 h, respectively. ( A , B ) Patterns and quantifications for annexin V/7AAD analysis. The apoptosis (%) is annexin V-positive (%). For multi-comparisons, the treatments marked without repeated characters (a to e) differ significantly ( p < 0.05). The data were plotted as the mean ± SD ( n = 3).

    Article Snippet: Its matched Cell Signaling Technology antibody was marked with Alexa Fluor 488 (1:10,000 dilution) and 7AAD staining (5 μg/mL) for the flow cytometry and FlowJo analysis.

    Techniques: