rabbit anti ctmp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti ctmp
    Treating differentiated murine C2C12 myotubes with 100 <t>ng/mL</t> <t>TNFα</t> for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, <t>CTMP</t> expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.
    Rabbit Anti Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ctmp/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti ctmp - by Bioz Stars, 2023-02
    91/100 stars

    Images

    1) Product Images from "Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis"

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40553-2

    Treating differentiated murine C2C12 myotubes with 100 ng/mL TNFα for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, CTMP expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.
    Figure Legend Snippet: Treating differentiated murine C2C12 myotubes with 100 ng/mL TNFα for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, CTMP expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.

    Techniques Used: Expressing

    Knocking down CTMP in TNFα-treated differentiated C2C12 myotubes using siRNA rescued the decrease in Akt phosphorylation. Incubating differentiated TNFα-treated C2C12 myotubes with siRNA against CTMP for 73 hours significantly decreased CTMP expression and increased Akt phosphorylation, indicating a direct relationship between CTMP expression and Akt phosphorylation in this muscle atrophy model. Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.05.
    Figure Legend Snippet: Knocking down CTMP in TNFα-treated differentiated C2C12 myotubes using siRNA rescued the decrease in Akt phosphorylation. Incubating differentiated TNFα-treated C2C12 myotubes with siRNA against CTMP for 73 hours significantly decreased CTMP expression and increased Akt phosphorylation, indicating a direct relationship between CTMP expression and Akt phosphorylation in this muscle atrophy model. Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.05.

    Techniques Used: Expressing

    rabbit anti ctmp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti ctmp
    Treating differentiated murine C2C12 myotubes with 100 <t>ng/mL</t> <t>TNFα</t> for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, <t>CTMP</t> expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.
    Rabbit Anti Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ctmp/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti ctmp - by Bioz Stars, 2023-02
    91/100 stars

    Images

    1) Product Images from "Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis"

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40553-2

    Treating differentiated murine C2C12 myotubes with 100 ng/mL TNFα for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, CTMP expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.
    Figure Legend Snippet: Treating differentiated murine C2C12 myotubes with 100 ng/mL TNFα for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, CTMP expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.

    Techniques Used: Expressing

    Knocking down CTMP in TNFα-treated differentiated C2C12 myotubes using siRNA rescued the decrease in Akt phosphorylation. Incubating differentiated TNFα-treated C2C12 myotubes with siRNA against CTMP for 73 hours significantly decreased CTMP expression and increased Akt phosphorylation, indicating a direct relationship between CTMP expression and Akt phosphorylation in this muscle atrophy model. Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.05.
    Figure Legend Snippet: Knocking down CTMP in TNFα-treated differentiated C2C12 myotubes using siRNA rescued the decrease in Akt phosphorylation. Incubating differentiated TNFα-treated C2C12 myotubes with siRNA against CTMP for 73 hours significantly decreased CTMP expression and increased Akt phosphorylation, indicating a direct relationship between CTMP expression and Akt phosphorylation in this muscle atrophy model. Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.05.

    Techniques Used: Expressing

    antibody against ctmp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibody against ctmp
    Univariate and multivariate Cox regression analysis for patients (n=369)
    Antibody Against Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against ctmp/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against ctmp - by Bioz Stars, 2023-02
    91/100 stars

    Images

    1) Product Images from "CTMP, a predictive biomarker for trastuzumab resistance in HER2-enriched breast cancer patient"

    Article Title: CTMP, a predictive biomarker for trastuzumab resistance in HER2-enriched breast cancer patient

    Journal: Oncotarget

    doi: 10.18632/oncotarget.10719

    Univariate and multivariate Cox regression analysis for patients (n=369)
    Figure Legend Snippet: Univariate and multivariate Cox regression analysis for patients (n=369)

    Techniques Used:

    A . CTMP, HER2 and AKT activity were analyzed in SkBR3 and BT-483 by Western blot. B . After 5 days trastuzumab treatment to SkBR3 and BT-483 cells, proliferation was examined using the MTT assay. Ectopic overexpression of different levels of CTMP in SkBR3 cells C . or knockdown of CTMP in BT-483 cells D . was used to modulate trastuzumab response as measured by the MTT assay. E . Western blots were performed to evaluate AKT, ERK and PDK1 and their activation in SkBR3 cells after transfection with GFP or GFP-CTMP. mean ± SEM; *, P < 0.05; ***, P < 0.001; n.s., no significance.
    Figure Legend Snippet: A . CTMP, HER2 and AKT activity were analyzed in SkBR3 and BT-483 by Western blot. B . After 5 days trastuzumab treatment to SkBR3 and BT-483 cells, proliferation was examined using the MTT assay. Ectopic overexpression of different levels of CTMP in SkBR3 cells C . or knockdown of CTMP in BT-483 cells D . was used to modulate trastuzumab response as measured by the MTT assay. E . Western blots were performed to evaluate AKT, ERK and PDK1 and their activation in SkBR3 cells after transfection with GFP or GFP-CTMP. mean ± SEM; *, P < 0.05; ***, P < 0.001; n.s., no significance.

    Techniques Used: Activity Assay, Western Blot, MTT Assay, Over Expression, Activation Assay, Transfection

    Western-blot was performed to determine AKT activity in CTMP-overexpressing SkBR3 (A) and in CTMP-silenced BT-483 cells (B) after trastuzumab treatment for 24 hours. C . CTMP-overexpressing SkBR3 and BT483 were treated with trastuzumab with or without AKT inhibitor IV, rapamycin and PD98058 for 72 hours and cell proliferation ability was determined by the MTT assay.
    Figure Legend Snippet: Western-blot was performed to determine AKT activity in CTMP-overexpressing SkBR3 (A) and in CTMP-silenced BT-483 cells (B) after trastuzumab treatment for 24 hours. C . CTMP-overexpressing SkBR3 and BT483 were treated with trastuzumab with or without AKT inhibitor IV, rapamycin and PD98058 for 72 hours and cell proliferation ability was determined by the MTT assay.

    Techniques Used: Western Blot, Activity Assay, MTT Assay

    A . CTMP, p-AKT and HER2 expression level were examined in HER2-enriched patients by IHC. In each sample, 100 cells were scored from 0 to 3 and the sum was taken as the staining index. B . The expression levels were compared between trastuzumab responders and non-responders by student t test. C . Person's correlation was used to analyze the correlation between CTMP and p473-AKT. PD: progressing disease; NED: non-evidence of disease; mean ± SEM; *, P<0.05.
    Figure Legend Snippet: A . CTMP, p-AKT and HER2 expression level were examined in HER2-enriched patients by IHC. In each sample, 100 cells were scored from 0 to 3 and the sum was taken as the staining index. B . The expression levels were compared between trastuzumab responders and non-responders by student t test. C . Person's correlation was used to analyze the correlation between CTMP and p473-AKT. PD: progressing disease; NED: non-evidence of disease; mean ± SEM; *, P<0.05.

    Techniques Used: Expressing, Staining

    anti ctmp  (Cell Signaling Technology Inc)


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  • 91

    Structured Review

    Cell Signaling Technology Inc anti ctmp
    Anti Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ctmp/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    anti ctmp - by Bioz Stars, 2023-02
    91/100 stars

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    crmp 4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc crmp 4
    Expression of <t>CRMP-4</t> in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.
    Crmp 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crmp 4/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    crmp 4 - by Bioz Stars, 2023-02
    91/100 stars

    Images

    1) Product Images from "Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth"

    Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2016.3588

    Expression of CRMP-4 in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.
    Figure Legend Snippet: Expression of CRMP-4 in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.

    Techniques Used: Expressing, Western Blot, Standard Deviation, Immunohistochemical staining, Staining

    Verification of knockdown CRMP-4 expression by lentivirus. (A) SW480 cells infected with lentivirus. (B) Reverse transcription-polymerase chain reaction and western blot analysis. GAPDH was used as an internal control. CRMP-4, collapsin response mediator protein 4; lv-si-CRMP-4, SW480 cells infected with lv-si-CRMP-4 lentivirus; lv-si-scramble, SW480 cells infected with lv-si-scramble lentivirus.
    Figure Legend Snippet: Verification of knockdown CRMP-4 expression by lentivirus. (A) SW480 cells infected with lentivirus. (B) Reverse transcription-polymerase chain reaction and western blot analysis. GAPDH was used as an internal control. CRMP-4, collapsin response mediator protein 4; lv-si-CRMP-4, SW480 cells infected with lv-si-CRMP-4 lentivirus; lv-si-scramble, SW480 cells infected with lv-si-scramble lentivirus.

    Techniques Used: Expressing, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Downregulation of CRMP-4 inhibits the proliferation of SW480 cells. (A) Viability of SW480 cells were evaluated by MTT assay. (B) Proliferation of SW480 cells were evaluated by BrdU incorporation. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4; OD, optical density.
    Figure Legend Snippet: Downregulation of CRMP-4 inhibits the proliferation of SW480 cells. (A) Viability of SW480 cells were evaluated by MTT assay. (B) Proliferation of SW480 cells were evaluated by BrdU incorporation. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4; OD, optical density.

    Techniques Used: MTT Assay, BrdU Incorporation Assay, Standard Deviation

    Colon tumor sizes in each group. (A) Colon tumor of mice injected with si-scramble cells or si-CRMP-4 cells after 4 weeks of injection. (B) Colon tumor size in each group after lentivirus injection at 1, 2, 3 and 4 weeks, respectively. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4.
    Figure Legend Snippet: Colon tumor sizes in each group. (A) Colon tumor of mice injected with si-scramble cells or si-CRMP-4 cells after 4 weeks of injection. (B) Colon tumor size in each group after lentivirus injection at 1, 2, 3 and 4 weeks, respectively. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4.

    Techniques Used: Injection, Standard Deviation

    ctmp antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ctmp antibody
    Ctmp Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctmp antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    ctmp antibody - by Bioz Stars, 2023-02
    91/100 stars

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    anti ctmp antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ctmp antibody
    ( A <t>)</t> <t>Immunohistochemical</t> <t>CTMP</t> expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.
    Anti Ctmp Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ctmp antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ctmp antibody - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Carboxyl-Terminal Modulator Protein Positively Acts as an Oncogenic Driver in Head and Neck Squamous Cell Carcinoma via Regulating Akt phosphorylation"

    Article Title: Carboxyl-Terminal Modulator Protein Positively Acts as an Oncogenic Driver in Head and Neck Squamous Cell Carcinoma via Regulating Akt phosphorylation

    Journal: Scientific Reports

    doi: 10.1038/srep28503

    ( A ) Immunohistochemical CTMP expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.
    Figure Legend Snippet: ( A ) Immunohistochemical CTMP expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.

    Techniques Used: Immunohistochemical staining, Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ctmp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ctmp
    Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ctmp antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ctmp antibody
    Anti Ctmp Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ctmp antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    anti ctmp antibody - by Bioz Stars, 2023-02
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    ctmp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ctmp
    Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    total c terminal modulator protein ctmp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc total c terminal modulator protein ctmp
    Total C Terminal Modulator Protein Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit anti ctmp
    Treating differentiated murine C2C12 myotubes with 100 <t>ng/mL</t> <t>TNFα</t> for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, <t>CTMP</t> expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.
    Rabbit Anti Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ctmp/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc antibody against ctmp
    Univariate and multivariate Cox regression analysis for patients (n=369)
    Antibody Against Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti ctmp
    Univariate and multivariate Cox regression analysis for patients (n=369)
    Anti Ctmp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ctmp/product/Cell Signaling Technology Inc
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    Expression of <t>CRMP-4</t> in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.
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    Expression of <t>CRMP-4</t> in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.
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    ( A <t>)</t> <t>Immunohistochemical</t> <t>CTMP</t> expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.
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    ( A <t>)</t> <t>Immunohistochemical</t> <t>CTMP</t> expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.
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    ( A <t>)</t> <t>Immunohistochemical</t> <t>CTMP</t> expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.
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    Image Search Results


    Treating differentiated murine C2C12 myotubes with 100 ng/mL TNFα for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, CTMP expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.

    Journal: Scientific Reports

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis

    doi: 10.1038/s41598-019-40553-2

    Figure Lengend Snippet: Treating differentiated murine C2C12 myotubes with 100 ng/mL TNFα for 24 hrs induced similar intracellular signaling pathway changes as observed in late-stage mSOD1 G93A mouse muscle tissue. Akt phosphorylation decreased following TNFα treatment ( A , E ). Meanwhile, PTEN expression was downregulated, suggesting it may not be a key modulator of Akt phosphorylation ( B , E ). However, CTMP expression significantly increased ( C , E ), as seen in the ALS mouse muscle. FOXO1 phosphorylation decreased ( D , E ), allowing it to permit transcription of atrophy genes such as Atrogin-1 , which was significantly upregulated ( F ). Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.01; *** P < 0.001.

    Article Snippet: Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-Akt Ser473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls.

    Techniques: Expressing

    Knocking down CTMP in TNFα-treated differentiated C2C12 myotubes using siRNA rescued the decrease in Akt phosphorylation. Incubating differentiated TNFα-treated C2C12 myotubes with siRNA against CTMP for 73 hours significantly decreased CTMP expression and increased Akt phosphorylation, indicating a direct relationship between CTMP expression and Akt phosphorylation in this muscle atrophy model. Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.05.

    Journal: Scientific Reports

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis

    doi: 10.1038/s41598-019-40553-2

    Figure Lengend Snippet: Knocking down CTMP in TNFα-treated differentiated C2C12 myotubes using siRNA rescued the decrease in Akt phosphorylation. Incubating differentiated TNFα-treated C2C12 myotubes with siRNA against CTMP for 73 hours significantly decreased CTMP expression and increased Akt phosphorylation, indicating a direct relationship between CTMP expression and Akt phosphorylation in this muscle atrophy model. Blot images are cropped from different membranes used for data collection and analysis (See Supplemental Figures). * P < 0.05.

    Article Snippet: Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-Akt Ser473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls.

    Techniques: Expressing

    Univariate and multivariate Cox regression analysis for patients (n=369)

    Journal: Oncotarget

    Article Title: CTMP, a predictive biomarker for trastuzumab resistance in HER2-enriched breast cancer patient

    doi: 10.18632/oncotarget.10719

    Figure Lengend Snippet: Univariate and multivariate Cox regression analysis for patients (n=369)

    Article Snippet: After blocking, the slides were incubated with primary antibody against CTMP (Cell signaling), AKT-p473 (Cell signaling) and HER2 followed by biotin-conjugated secondary antibody, polymer-HRP and diaminobenzidine tetrahydroxychloride (DAB) solution.

    Techniques:

    A . CTMP, HER2 and AKT activity were analyzed in SkBR3 and BT-483 by Western blot. B . After 5 days trastuzumab treatment to SkBR3 and BT-483 cells, proliferation was examined using the MTT assay. Ectopic overexpression of different levels of CTMP in SkBR3 cells C . or knockdown of CTMP in BT-483 cells D . was used to modulate trastuzumab response as measured by the MTT assay. E . Western blots were performed to evaluate AKT, ERK and PDK1 and their activation in SkBR3 cells after transfection with GFP or GFP-CTMP. mean ± SEM; *, P < 0.05; ***, P < 0.001; n.s., no significance.

    Journal: Oncotarget

    Article Title: CTMP, a predictive biomarker for trastuzumab resistance in HER2-enriched breast cancer patient

    doi: 10.18632/oncotarget.10719

    Figure Lengend Snippet: A . CTMP, HER2 and AKT activity were analyzed in SkBR3 and BT-483 by Western blot. B . After 5 days trastuzumab treatment to SkBR3 and BT-483 cells, proliferation was examined using the MTT assay. Ectopic overexpression of different levels of CTMP in SkBR3 cells C . or knockdown of CTMP in BT-483 cells D . was used to modulate trastuzumab response as measured by the MTT assay. E . Western blots were performed to evaluate AKT, ERK and PDK1 and their activation in SkBR3 cells after transfection with GFP or GFP-CTMP. mean ± SEM; *, P < 0.05; ***, P < 0.001; n.s., no significance.

    Article Snippet: After blocking, the slides were incubated with primary antibody against CTMP (Cell signaling), AKT-p473 (Cell signaling) and HER2 followed by biotin-conjugated secondary antibody, polymer-HRP and diaminobenzidine tetrahydroxychloride (DAB) solution.

    Techniques: Activity Assay, Western Blot, MTT Assay, Over Expression, Activation Assay, Transfection

    Western-blot was performed to determine AKT activity in CTMP-overexpressing SkBR3 (A) and in CTMP-silenced BT-483 cells (B) after trastuzumab treatment for 24 hours. C . CTMP-overexpressing SkBR3 and BT483 were treated with trastuzumab with or without AKT inhibitor IV, rapamycin and PD98058 for 72 hours and cell proliferation ability was determined by the MTT assay.

    Journal: Oncotarget

    Article Title: CTMP, a predictive biomarker for trastuzumab resistance in HER2-enriched breast cancer patient

    doi: 10.18632/oncotarget.10719

    Figure Lengend Snippet: Western-blot was performed to determine AKT activity in CTMP-overexpressing SkBR3 (A) and in CTMP-silenced BT-483 cells (B) after trastuzumab treatment for 24 hours. C . CTMP-overexpressing SkBR3 and BT483 were treated with trastuzumab with or without AKT inhibitor IV, rapamycin and PD98058 for 72 hours and cell proliferation ability was determined by the MTT assay.

    Article Snippet: After blocking, the slides were incubated with primary antibody against CTMP (Cell signaling), AKT-p473 (Cell signaling) and HER2 followed by biotin-conjugated secondary antibody, polymer-HRP and diaminobenzidine tetrahydroxychloride (DAB) solution.

    Techniques: Western Blot, Activity Assay, MTT Assay

    A . CTMP, p-AKT and HER2 expression level were examined in HER2-enriched patients by IHC. In each sample, 100 cells were scored from 0 to 3 and the sum was taken as the staining index. B . The expression levels were compared between trastuzumab responders and non-responders by student t test. C . Person's correlation was used to analyze the correlation between CTMP and p473-AKT. PD: progressing disease; NED: non-evidence of disease; mean ± SEM; *, P<0.05.

    Journal: Oncotarget

    Article Title: CTMP, a predictive biomarker for trastuzumab resistance in HER2-enriched breast cancer patient

    doi: 10.18632/oncotarget.10719

    Figure Lengend Snippet: A . CTMP, p-AKT and HER2 expression level were examined in HER2-enriched patients by IHC. In each sample, 100 cells were scored from 0 to 3 and the sum was taken as the staining index. B . The expression levels were compared between trastuzumab responders and non-responders by student t test. C . Person's correlation was used to analyze the correlation between CTMP and p473-AKT. PD: progressing disease; NED: non-evidence of disease; mean ± SEM; *, P<0.05.

    Article Snippet: After blocking, the slides were incubated with primary antibody against CTMP (Cell signaling), AKT-p473 (Cell signaling) and HER2 followed by biotin-conjugated secondary antibody, polymer-HRP and diaminobenzidine tetrahydroxychloride (DAB) solution.

    Techniques: Expressing, Staining

    Expression of CRMP-4 in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

    doi: 10.3892/etm.2016.3588

    Figure Lengend Snippet: Expression of CRMP-4 in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.

    Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Expressing, Western Blot, Standard Deviation, Immunohistochemical staining, Staining

    Verification of knockdown CRMP-4 expression by lentivirus. (A) SW480 cells infected with lentivirus. (B) Reverse transcription-polymerase chain reaction and western blot analysis. GAPDH was used as an internal control. CRMP-4, collapsin response mediator protein 4; lv-si-CRMP-4, SW480 cells infected with lv-si-CRMP-4 lentivirus; lv-si-scramble, SW480 cells infected with lv-si-scramble lentivirus.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

    doi: 10.3892/etm.2016.3588

    Figure Lengend Snippet: Verification of knockdown CRMP-4 expression by lentivirus. (A) SW480 cells infected with lentivirus. (B) Reverse transcription-polymerase chain reaction and western blot analysis. GAPDH was used as an internal control. CRMP-4, collapsin response mediator protein 4; lv-si-CRMP-4, SW480 cells infected with lv-si-CRMP-4 lentivirus; lv-si-scramble, SW480 cells infected with lv-si-scramble lentivirus.

    Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Expressing, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Downregulation of CRMP-4 inhibits the proliferation of SW480 cells. (A) Viability of SW480 cells were evaluated by MTT assay. (B) Proliferation of SW480 cells were evaluated by BrdU incorporation. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4; OD, optical density.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

    doi: 10.3892/etm.2016.3588

    Figure Lengend Snippet: Downregulation of CRMP-4 inhibits the proliferation of SW480 cells. (A) Viability of SW480 cells were evaluated by MTT assay. (B) Proliferation of SW480 cells were evaluated by BrdU incorporation. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4; OD, optical density.

    Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: MTT Assay, BrdU Incorporation Assay, Standard Deviation

    Colon tumor sizes in each group. (A) Colon tumor of mice injected with si-scramble cells or si-CRMP-4 cells after 4 weeks of injection. (B) Colon tumor size in each group after lentivirus injection at 1, 2, 3 and 4 weeks, respectively. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

    doi: 10.3892/etm.2016.3588

    Figure Lengend Snippet: Colon tumor sizes in each group. (A) Colon tumor of mice injected with si-scramble cells or si-CRMP-4 cells after 4 weeks of injection. (B) Colon tumor size in each group after lentivirus injection at 1, 2, 3 and 4 weeks, respectively. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4.

    Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Injection, Standard Deviation

    ( A ) Immunohistochemical CTMP expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.

    Journal: Scientific Reports

    Article Title: Carboxyl-Terminal Modulator Protein Positively Acts as an Oncogenic Driver in Head and Neck Squamous Cell Carcinoma via Regulating Akt phosphorylation

    doi: 10.1038/srep28503

    Figure Lengend Snippet: ( A ) Immunohistochemical CTMP expression. Representative immunohistochemical images of: (a) no staining intensity, (b) weak staining intensity, (c) moderate staining intensity, and (d) strong staining intensity in tumor samples from 119 HNSCC patients. (a) and (b) were classified as ‘low CTMP expression’, whereas (c) and (d) were classified as ‘high CTMP expression’ (100x magnification). ( B , C ) CTMP expression levels in the total cell lysate acquired from normal (N)–tumor (T) paired clinical specimens were examined through ( B ) RT-PCR and ( C ) Western blot assays.

    Article Snippet: Immunohistochemical (IHC) staining using an anti-CTMP antibody (#4612, 1:50; Cell Signaling Technology Inc., Danvers, MA, USA) was performed using a 3,3′-diaminobenzidine (DAB) peroxidase substrate kit according to the manufacturer’s instructions (Sigma-Aldrich, St. Louis, MO, USA).

    Techniques: Immunohistochemical staining, Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot