mouse anti cleaved caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti cleaved caspase 7
    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, <t>caspase-7</t> (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.
    Mouse Anti Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti cleaved caspase 7/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    mouse anti cleaved caspase 7 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Targeted Mutation of the Mouse Grp94 Gene Disrupts Development and Perturbs Endoplasmic Reticulum Stress Signaling"

    Article Title: Targeted Mutation of the Mouse Grp94 Gene Disrupts Development and Perturbs Endoplasmic Reticulum Stress Signaling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010852

    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, caspase-7 (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.
    Figure Legend Snippet: (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, caspase-7 (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.

    Techniques Used: Western Blot

    mouse anti cleaved caspase 7  (Cell Signaling Technology Inc)


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  • 94

    Structured Review

    Cell Signaling Technology Inc mouse anti cleaved caspase 7
    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, <t>caspase-7</t> (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.
    Mouse Anti Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti cleaved caspase 7/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "Targeted Mutation of the Mouse Grp94 Gene Disrupts Development and Perturbs Endoplasmic Reticulum Stress Signaling"

    Article Title: Targeted Mutation of the Mouse Grp94 Gene Disrupts Development and Perturbs Endoplasmic Reticulum Stress Signaling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010852

    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, caspase-7 (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.
    Figure Legend Snippet: (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, caspase-7 (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.

    Techniques Used: Western Blot

    cleaved caspase 7  (Cell Signaling Technology Inc)


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  • 94

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    Cell Signaling Technology Inc cleaved caspase 7
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7/product/Cell Signaling Technology Inc
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    cleaved caspase 7  (Cell Signaling Technology Inc)


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  • 94

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    Cell Signaling Technology Inc cleaved caspase 7
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    cleaved caspase 7  (Cell Signaling Technology Inc)


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  • 94

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    Cell Signaling Technology Inc cleaved caspase 7
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7/product/Cell Signaling Technology Inc
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    ranibizumab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ranibizumab
    Demographic characteristics of all treatment‐naïve CRVO eyes commencing <t> ranibizumab </t> or aflibercept treatment 2014–2019.
    Ranibizumab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "12‐month outcomes of ranibizumab versus aflibercept for macular oedema in central retinal vein occlusion: data from the FRB! registry"

    Article Title: 12‐month outcomes of ranibizumab versus aflibercept for macular oedema in central retinal vein occlusion: data from the FRB! registry

    Journal: Acta Ophthalmologica

    doi: 10.1111/aos.15014

    Demographic characteristics of all treatment‐naïve CRVO eyes commencing  ranibizumab  or aflibercept treatment 2014–2019.
    Figure Legend Snippet: Demographic characteristics of all treatment‐naïve CRVO eyes commencing ranibizumab or aflibercept treatment 2014–2019.

    Techniques Used:

    12‐month outcomes in all eyes and stratified by anti‐VEGF agent received. Significant p‐values comparing  ranibizumab  and aflibercept are highlighted in bold.
    Figure Legend Snippet: 12‐month outcomes in all eyes and stratified by anti‐VEGF agent received. Significant p‐values comparing ranibizumab and aflibercept are highlighted in bold.

    Techniques Used:

    cleaved caspase 7 asp198 d6h1 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7 asp198 d6h1 rabbit mab
    List of antibodies used.
    Cleaved Caspase 7 Asp198 D6h1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7 asp198 d6h1 rabbit mab/product/Cell Signaling Technology Inc
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    1) Product Images from "PRKAR1B-AS2 Long Noncoding RNA Promotes Tumorigenesis, Survival, and Chemoresistance via the PI3K/AKT/mTOR Pathway"

    Article Title: PRKAR1B-AS2 Long Noncoding RNA Promotes Tumorigenesis, Survival, and Chemoresistance via the PI3K/AKT/mTOR Pathway

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22041882

    List of antibodies used.
    Figure Legend Snippet: List of antibodies used.

    Techniques Used:

    cleaved caspase 7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7/product/Cell Signaling Technology Inc
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    cleaved caspase 7 asp198  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7 asp198
    Cleaved Caspase 7 Asp198, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7 asp198/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    cleaved caspase 7 asp198  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7 asp198
    Primary antibodies and dilutions used for In-Cell Western assays
    Cleaved Caspase 7 Asp198, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7 asp198/product/Cell Signaling Technology Inc
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    1) Product Images from "Human proximal tubular cells can form calcium phosphate deposits in osteogenic culture: role of cell death and osteoblast-like transdifferentiation"

    Article Title: Human proximal tubular cells can form calcium phosphate deposits in osteogenic culture: role of cell death and osteoblast-like transdifferentiation

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-019-0138-x

    Primary antibodies and dilutions used for In-Cell Western assays
    Figure Legend Snippet: Primary antibodies and dilutions used for In-Cell Western assays

    Techniques Used: In-Cell ELISA

    cleaved caspase 7 asp198  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 7 asp198

    Cleaved Caspase 7 Asp198, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7 asp198/product/Cell Signaling Technology Inc
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    1) Product Images from "HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties"

    Article Title: HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties

    Journal: eLife

    doi: 10.7554/eLife.33947


    Figure Legend Snippet:

    Techniques Used: Clone Assay, Variant Assay, shRNA, Sequencing, Amplification, Transfection, Construct, Flow Cytometry, Western Blot, Mutagenesis, Recombinant, Plasmid Preparation, Blocking Assay, Chromatin Immunoprecipitation, Magnetic Beads, Luciferase, SYBR Green Assay, Binding Assay, Software

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    Cell Signaling Technology Inc mouse anti cleaved caspase 7
    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, <t>caspase-7</t> (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.
    Mouse Anti Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti cleaved caspase 7/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc cleaved caspase 7
    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, <t>caspase-7</t> (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.
    Cleaved Caspase 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc ranibizumab
    Demographic characteristics of all treatment‐naïve CRVO eyes commencing <t> ranibizumab </t> or aflibercept treatment 2014–2019.
    Ranibizumab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ranibizumab/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc cleaved caspase 7 asp198 d6h1 rabbit mab
    List of antibodies used.
    Cleaved Caspase 7 Asp198 D6h1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7 asp198 d6h1 rabbit mab/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc cleaved caspase 7 asp198
    List of antibodies used.
    Cleaved Caspase 7 Asp198, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 7 asp198/product/Cell Signaling Technology Inc
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    Image Search Results


    (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, caspase-7 (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.

    Journal: PLoS ONE

    Article Title: Targeted Mutation of the Mouse Grp94 Gene Disrupts Development and Perturbs Endoplasmic Reticulum Stress Signaling

    doi: 10.1371/journal.pone.0010852

    Figure Lengend Snippet: (A) Representative Western blot results on effect of GRP94 depletion on cell proliferation and apoptosis. Grp94 +/+ and −/− ESCs were treated with 300 nM Tg for the indicated time (hr). The whole cell lysates were subjected to Western blot with antibodies against PCNA, caspase-7 (C-7) and β-actin. Both pro C-7 (the uncleaved form) and cleaved C-7 (active form) were detected by the anti-C-7 antibody. The cleaved C-7 protein level was quantitated and normalized against β-actin. The level of cleaved C-7 in Grp94 +/+ ESCs treated with Tg for 4 hr was set as 1. (B) The same as in (A) with 1.5 µg/ml Tunicamycin (Tu) treated cells. The Western blots were performed with antibodies against cleaved caspase-3 (C-3) and β-actin. The cleaved C-3 protein level was quantitated and normalized against β-actin. The level of cleaved C-3 in Grp94 +/+ ESCs without Tu treatment was set as 1. These experiments were repeated two times.

    Article Snippet: The primary antibodies used included the following: mouse anti-KDEL(10C3) (1∶1000), rat anti-GRP94 (1∶2500), rabbit anti-calnexin (1∶2000), and rabbit anti-calreticulin (1∶2000) from Stressgen; goat anti-GRP78(C20) (1∶5000), rabbit anti-PERK (H-300) (1∶500), rabbit anti-ATF4 (CREB-2) (1∶1000), goat anti-HSP70 (K-20) (1∶1000), mouse anti-PCNA (PC10) (1∶1000), mouse anti-CHOP (1∶1000), goat anti-EDEM (1∶500) and rabbit anti-XBP1 (1∶500) from Santa Cruz Biotechnology; rabbit anti-phospho-PERK (Thr980) (1∶500), mouse anti-cleaved-caspase-7 (Asp198), rabbit anti-phospho-eIF2α (1∶1000), rabbit anti- eIF2α (1∶1000) and rabbit anti-cleaved caspase-3 (Asp175) (1∶500) from Cell Signaling; rabbit anti-PDI (1∶2000) from Assay Designs; mouse anti-β-actin (1∶5000) from Sigma-Aldrich; and rabbit anti-p62 (SQSTM1) (1∶1000) from BIOMOL.

    Techniques: Western Blot

    Demographic characteristics of all treatment‐naïve CRVO eyes commencing  ranibizumab  or aflibercept treatment 2014–2019.

    Journal: Acta Ophthalmologica

    Article Title: 12‐month outcomes of ranibizumab versus aflibercept for macular oedema in central retinal vein occlusion: data from the FRB! registry

    doi: 10.1111/aos.15014

    Figure Lengend Snippet: Demographic characteristics of all treatment‐naïve CRVO eyes commencing ranibizumab or aflibercept treatment 2014–2019.

    Article Snippet: Baseline VA (SD) was somewhat better in aflibercept‐ versus ranibizumab‐treated eyes (42.5 ± 25.5 letters versus 36.9 ± 26 letters; p = 0.07) with similar CST (614 (240) μm versus 616 (234) μm: p = 0.95).

    Techniques:

    12‐month outcomes in all eyes and stratified by anti‐VEGF agent received. Significant p‐values comparing  ranibizumab  and aflibercept are highlighted in bold.

    Journal: Acta Ophthalmologica

    Article Title: 12‐month outcomes of ranibizumab versus aflibercept for macular oedema in central retinal vein occlusion: data from the FRB! registry

    doi: 10.1111/aos.15014

    Figure Lengend Snippet: 12‐month outcomes in all eyes and stratified by anti‐VEGF agent received. Significant p‐values comparing ranibizumab and aflibercept are highlighted in bold.

    Article Snippet: Baseline VA (SD) was somewhat better in aflibercept‐ versus ranibizumab‐treated eyes (42.5 ± 25.5 letters versus 36.9 ± 26 letters; p = 0.07) with similar CST (614 (240) μm versus 616 (234) μm: p = 0.95).

    Techniques:

    List of antibodies used.

    Journal: International Journal of Molecular Sciences

    Article Title: PRKAR1B-AS2 Long Noncoding RNA Promotes Tumorigenesis, Survival, and Chemoresistance via the PI3K/AKT/mTOR Pathway

    doi: 10.3390/ijms22041882

    Figure Lengend Snippet: List of antibodies used.

    Article Snippet: Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb , Cell Signaling Technology, Danvers, MA, USA (8438) , 1:400 , Anti-rabbit IgG, HRP-linked Antibody , Cell Signaling Technology, Danvers, MA, USA (7074) , 1:2000.

    Techniques: