Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Metformin Protects against Spinal Cord Injury and Cell Pyroptosis via AMPK/NLRP3 Inflammasome Pathway

doi: 10.1155/2022/3634908

Figure Lengend Snippet: Metformin activates the AMPK/NLRP3 signaling pathway in spinal cord neurons. (a–c) Representative Western blots and quantification data of the levels of p-AMPK and p-ACC in the spinal cord neurons from each group. All data represented the mean ± SD, ∗∗∗ P < 0.001, n = 3. (d–h) Representative Western blots and quantification data of the expression of NLRP3, ASC, Caspase 1, and IL-1 β in the spinal cord neurons from each group. All data represent the mean ± SD, ∗∗∗ P < 0.001, n = 3. (i, j) Immunofluorescence examination was utilized to recognize the staining power of antibodies focusing on NeuN and p-AMPK. Scale bars represent 40 μ m. ∗∗∗ P < 0.001, n = 3.

Article Snippet: The accompanying antibodies were utilized for recognition in this experiment: antiphosphorylated AMPK (p-AMPK, 1 : 1000, Cell Signaling Technology, USA), anti-AMPK (1 : 1000, Cell Signaling Technology), anti-p-ACC (1 : 1000, Cell Signaling Technology), anti-ACC (1 : 1000, Cell Signaling Technology), anti-NLRP3 (1 : 1000, Cell Signaling Technology), anti-ASC (1 : 1000, Abcam, UK), anti-Caspase 1 (1 : 1000, Abcam), anti-IL-1 β (1 : 1000, Abcam), anti-GAPDH (1 : 1000, Proteintech, USA), HRP Affinipure Goat Anti-Mouse IgG (1 : 1000, Proteintech), and HRP Affinipure Goat Anti-Rabbit IgG (1 : 1000, Proteintech).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Metformin Protects against Spinal Cord Injury and Cell Pyroptosis via AMPK/NLRP3 Inflammasome Pathway

doi: 10.1155/2022/3634908

Figure Lengend Snippet: Metformin activates the AMPK/NLRP3 signaling pathway and inhibits inflammation after SCI. (a–c) Representative Western blots and quantification data of the levels of p-AMPK and p-ACC after SCI from each group. All data represent the mean ± SD, ∗∗∗ P < 0.001, n = 3. (d–h) Representative Western blots and quantification data of the expression of NLRP3, ASC, Caspase 1, and IL-1 β after SCI from each group. All data represent the mean ± SD, ∗∗∗ P < 0.001, n = 3. (i, j) Immunohistochemical detection of the levels of NLRP3 protein in each group of rats at 7 days after SCI. Scale bars represent 100 μ m. ∗∗∗ P < 0.001, n = 3. (k–m) ELISA was used to detect the expression of IL-1 β , IL-6, and TNF- α in the spinal cord tissue homogenate from each group. All data represent the mean ± SD, ∗∗∗ P < 0.001, n = 3.

Article Snippet: The accompanying antibodies were utilized for recognition in this experiment: antiphosphorylated AMPK (p-AMPK, 1 : 1000, Cell Signaling Technology, USA), anti-AMPK (1 : 1000, Cell Signaling Technology), anti-p-ACC (1 : 1000, Cell Signaling Technology), anti-ACC (1 : 1000, Cell Signaling Technology), anti-NLRP3 (1 : 1000, Cell Signaling Technology), anti-ASC (1 : 1000, Abcam, UK), anti-Caspase 1 (1 : 1000, Abcam), anti-IL-1 β (1 : 1000, Abcam), anti-GAPDH (1 : 1000, Proteintech, USA), HRP Affinipure Goat Anti-Mouse IgG (1 : 1000, Proteintech), and HRP Affinipure Goat Anti-Rabbit IgG (1 : 1000, Proteintech).

Techniques: Western Blot, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Keap1-Knockdown Decreases Fasting-Induced Fatty Liver via Altered Lipid Metabolism and Decreased Fatty Acid Mobilization from Adipose Tissue

doi: 10.1371/journal.pone.0079841

Figure Lengend Snippet: Immunoblot analysis of ( A ) Acc-1, Scd-1, and ( B ) total and p-AMPKα, total-AMPKβ1/2, and p-AMPKβ1 in livers of C57BL/6 (WT) and Keap1-KD (KD) mice. *, P<0.05, WT-Fasted compared with WT-Fed mice; #, P<0.05, KD-Fasted compared with KD-Fed mice; §, P<0.05, KD-Fasted compared with WT-Fasted mice. ( C ) p-AMPKα levels were increased in primary hepatocytes isolated from C57BL/6 (WT) and Keap1-KD (KD) mice. &, P<0.05, KD compared with WT mice hepatocytes.

Article Snippet: Primary antibodies were directed against: total-AMPKα, AMPKα phosphorylated at Thr172, total-AMPKβ1/2, AMPKβ1 phosphorylated at Ser108, Acc-1, Scd-1, total-Akt, Akt phosphorylated at Ser473 and GAPDH (Cell Signaling Technology, Danvers, MA); and Glut4 (from Sigma-Aldrich, St. Louis, MO).

Techniques: Western Blot, Isolation