s rbd recombinant protein  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc s rbd recombinant protein
    Graphs show serum <t>anti-S-RBD</t> IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.
    S Rbd Recombinant Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s rbd recombinant protein/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s rbd recombinant protein - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Longitudinal analyses after COVID-19 recovery or prolonged infection reveal unique immunological signatures after repeated vaccinations"

    Article Title: Longitudinal analyses after COVID-19 recovery or prolonged infection reveal unique immunological signatures after repeated vaccinations

    Journal: medRxiv

    doi: 10.1101/2022.08.03.22278353

    Graphs show serum anti-S-RBD IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.
    Figure Legend Snippet: Graphs show serum anti-S-RBD IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.

    Techniques Used: Activity Assay, Infection

    (A) Concentration of anti-S-RBD IgM and IgG in the serum of a healthcare worker (indicated by # in Figure 1) upon infection. (B) Fluctuation of each neutralizing activity from onset to post-vaccination. (C) Representative FACS plots of cell population are shown in panels. The numbers indicate the positive rate of each cell population. Graphs show the absolute number of IgG + S-RBD + Bmems (D) and the frequency of lymphocyte (E), CD19 + CD27 + CD38 high , and IgG + Bmems (F). Each number indicates the mean.
    Figure Legend Snippet: (A) Concentration of anti-S-RBD IgM and IgG in the serum of a healthcare worker (indicated by # in Figure 1) upon infection. (B) Fluctuation of each neutralizing activity from onset to post-vaccination. (C) Representative FACS plots of cell population are shown in panels. The numbers indicate the positive rate of each cell population. Graphs show the absolute number of IgG + S-RBD + Bmems (D) and the frequency of lymphocyte (E), CD19 + CD27 + CD38 high , and IgG + Bmems (F). Each number indicates the mean.

    Techniques Used: Concentration Assay, Infection, Activity Assay

    s rbd recombinant protein  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Structured Review

    Cell Signaling Technology Inc s rbd recombinant protein
    Graphs show serum <t>anti-S-RBD</t> IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.
    S Rbd Recombinant Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s rbd recombinant protein/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s rbd recombinant protein - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Longitudinal analyses after COVID-19 recovery or prolonged infection reveal unique immunological signatures after repeated vaccinations"

    Article Title: Longitudinal analyses after COVID-19 recovery or prolonged infection reveal unique immunological signatures after repeated vaccinations

    Journal: medRxiv

    doi: 10.1101/2022.08.03.22278353

    Graphs show serum anti-S-RBD IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.
    Figure Legend Snippet: Graphs show serum anti-S-RBD IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.

    Techniques Used: Activity Assay, Infection

    (A) Concentration of anti-S-RBD IgM and IgG in the serum of a healthcare worker (indicated by # in Figure 1) upon infection. (B) Fluctuation of each neutralizing activity from onset to post-vaccination. (C) Representative FACS plots of cell population are shown in panels. The numbers indicate the positive rate of each cell population. Graphs show the absolute number of IgG + S-RBD + Bmems (D) and the frequency of lymphocyte (E), CD19 + CD27 + CD38 high , and IgG + Bmems (F). Each number indicates the mean.
    Figure Legend Snippet: (A) Concentration of anti-S-RBD IgM and IgG in the serum of a healthcare worker (indicated by # in Figure 1) upon infection. (B) Fluctuation of each neutralizing activity from onset to post-vaccination. (C) Representative FACS plots of cell population are shown in panels. The numbers indicate the positive rate of each cell population. Graphs show the absolute number of IgG + S-RBD + Bmems (D) and the frequency of lymphocyte (E), CD19 + CD27 + CD38 high , and IgG + Bmems (F). Each number indicates the mean.

    Techniques Used: Concentration Assay, Infection, Activity Assay

    receptor binding domain rbd  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc receptor binding domain rbd
    Study approach and distinct antibody responses to SARS-CoV-2 along with neutralization assay. (A) Outline delineating the experimental workflow. (B) Box plots showing the anti-SARS-CoV-2 <t>total,</t> <t>IgA,</t> IgM, and IgG antibody proportions in serum samples from controls, symptomatic- and asymptomatic-infected individuals and in contacts (C) . Pie chart showing the overall immunoglobulin response across subject groups. (D) Box plot depicting the percent inhibition of neutralizing antibodies in an in vitro spike <t>RBD-ACE2</t> interaction-based surrogate virus neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test. p-values are written against respective comparisons, where n = number of individuals; IgA, immunoglobulin A; IgM, immunoglobulin M; IgG, immunoglobulin G; control (n = 14), infected (n = 23), symptomatic (n = 10), asymptomatic (n = 13), and contact (n = 24).
    Receptor Binding Domain Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/receptor binding domain rbd/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    receptor binding domain rbd - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases"

    Article Title: Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.733539

    Study approach and distinct antibody responses to SARS-CoV-2 along with neutralization assay. (A) Outline delineating the experimental workflow. (B) Box plots showing the anti-SARS-CoV-2 total, IgA, IgM, and IgG antibody proportions in serum samples from controls, symptomatic- and asymptomatic-infected individuals and in contacts (C) . Pie chart showing the overall immunoglobulin response across subject groups. (D) Box plot depicting the percent inhibition of neutralizing antibodies in an in vitro spike RBD-ACE2 interaction-based surrogate virus neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test. p-values are written against respective comparisons, where n = number of individuals; IgA, immunoglobulin A; IgM, immunoglobulin M; IgG, immunoglobulin G; control (n = 14), infected (n = 23), symptomatic (n = 10), asymptomatic (n = 13), and contact (n = 24).
    Figure Legend Snippet: Study approach and distinct antibody responses to SARS-CoV-2 along with neutralization assay. (A) Outline delineating the experimental workflow. (B) Box plots showing the anti-SARS-CoV-2 total, IgA, IgM, and IgG antibody proportions in serum samples from controls, symptomatic- and asymptomatic-infected individuals and in contacts (C) . Pie chart showing the overall immunoglobulin response across subject groups. (D) Box plot depicting the percent inhibition of neutralizing antibodies in an in vitro spike RBD-ACE2 interaction-based surrogate virus neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test. p-values are written against respective comparisons, where n = number of individuals; IgA, immunoglobulin A; IgM, immunoglobulin M; IgG, immunoglobulin G; control (n = 14), infected (n = 23), symptomatic (n = 10), asymptomatic (n = 13), and contact (n = 24).

    Techniques Used: Neutralization, Infection, Inhibition, In Vitro

    rbd  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rbd
    A , Box plots showing the <t>anti-SARS-CoV-2</t> <t>IgA,</t> IgM and IgG antibody proportions in serum samples from control, infected and contact along with B , Serum cytokine levels in pg/ml (Eotaxin, G-CSF, IL-7, MIF and MIP 1-□) determined by bio plex. C , The area under the receiver-operating characteristic (ROC) curve of the cytokines having more than 0.8 AUC. D , Box plot depicting the percent inhibition of neutralizing antibodies in an in -vitro spike <t>RBD-ACE2</t> interaction based Surrogate Virus Neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test, p-values are written against respective comparisons. Where, n = number of individuals, IgA-Immunoglobulin A, IgM-Immunoglobulin M and IgG-Immunoglobulin G, control (n-14), infected (n-23, Symptomatic n-10 and Asymptomatic n-13) and contact (n-24).
    Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbd/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rbd - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "SARS-CoV-2 specific immune-signature in direct contacts of COVID-19 cases protect them from contracting disease: A Retrospective Study"

    Article Title: SARS-CoV-2 specific immune-signature in direct contacts of COVID-19 cases protect them from contracting disease: A Retrospective Study

    Journal: medRxiv

    doi: 10.1101/2021.03.11.21253367

    A , Box plots showing the anti-SARS-CoV-2 IgA, IgM and IgG antibody proportions in serum samples from control, infected and contact along with B , Serum cytokine levels in pg/ml (Eotaxin, G-CSF, IL-7, MIF and MIP 1-□) determined by bio plex. C , The area under the receiver-operating characteristic (ROC) curve of the cytokines having more than 0.8 AUC. D , Box plot depicting the percent inhibition of neutralizing antibodies in an in -vitro spike RBD-ACE2 interaction based Surrogate Virus Neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test, p-values are written against respective comparisons. Where, n = number of individuals, IgA-Immunoglobulin A, IgM-Immunoglobulin M and IgG-Immunoglobulin G, control (n-14), infected (n-23, Symptomatic n-10 and Asymptomatic n-13) and contact (n-24).
    Figure Legend Snippet: A , Box plots showing the anti-SARS-CoV-2 IgA, IgM and IgG antibody proportions in serum samples from control, infected and contact along with B , Serum cytokine levels in pg/ml (Eotaxin, G-CSF, IL-7, MIF and MIP 1-□) determined by bio plex. C , The area under the receiver-operating characteristic (ROC) curve of the cytokines having more than 0.8 AUC. D , Box plot depicting the percent inhibition of neutralizing antibodies in an in -vitro spike RBD-ACE2 interaction based Surrogate Virus Neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test, p-values are written against respective comparisons. Where, n = number of individuals, IgA-Immunoglobulin A, IgM-Immunoglobulin M and IgG-Immunoglobulin G, control (n-14), infected (n-23, Symptomatic n-10 and Asymptomatic n-13) and contact (n-24).

    Techniques Used: Infection, Inhibition, In Vitro, Neutralization

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    Cell Signaling Technology Inc s rbd recombinant protein
    Graphs show serum <t>anti-S-RBD</t> IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.
    S Rbd Recombinant Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s rbd recombinant protein/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s rbd recombinant protein - by Bioz Stars, 2023-02
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    94
    Cell Signaling Technology Inc receptor binding domain rbd
    Study approach and distinct antibody responses to SARS-CoV-2 along with neutralization assay. (A) Outline delineating the experimental workflow. (B) Box plots showing the anti-SARS-CoV-2 <t>total,</t> <t>IgA,</t> IgM, and IgG antibody proportions in serum samples from controls, symptomatic- and asymptomatic-infected individuals and in contacts (C) . Pie chart showing the overall immunoglobulin response across subject groups. (D) Box plot depicting the percent inhibition of neutralizing antibodies in an in vitro spike <t>RBD-ACE2</t> interaction-based surrogate virus neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test. p-values are written against respective comparisons, where n = number of individuals; IgA, immunoglobulin A; IgM, immunoglobulin M; IgG, immunoglobulin G; control (n = 14), infected (n = 23), symptomatic (n = 10), asymptomatic (n = 13), and contact (n = 24).
    Receptor Binding Domain Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/receptor binding domain rbd/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    receptor binding domain rbd - by Bioz Stars, 2023-02
    94/100 stars
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    Cell Signaling Technology Inc rbd
    A , Box plots showing the <t>anti-SARS-CoV-2</t> <t>IgA,</t> IgM and IgG antibody proportions in serum samples from control, infected and contact along with B , Serum cytokine levels in pg/ml (Eotaxin, G-CSF, IL-7, MIF and MIP 1-□) determined by bio plex. C , The area under the receiver-operating characteristic (ROC) curve of the cytokines having more than 0.8 AUC. D , Box plot depicting the percent inhibition of neutralizing antibodies in an in -vitro spike <t>RBD-ACE2</t> interaction based Surrogate Virus Neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test, p-values are written against respective comparisons. Where, n = number of individuals, IgA-Immunoglobulin A, IgM-Immunoglobulin M and IgG-Immunoglobulin G, control (n-14), infected (n-23, Symptomatic n-10 and Asymptomatic n-13) and contact (n-24).
    Rbd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbd/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rbd - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    Image Search Results


    Graphs show serum anti-S-RBD IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.

    Journal: medRxiv

    Article Title: Longitudinal analyses after COVID-19 recovery or prolonged infection reveal unique immunological signatures after repeated vaccinations

    doi: 10.1101/2022.08.03.22278353

    Figure Lengend Snippet: Graphs show serum anti-S-RBD IgM (A) and IgG (B) levels for each disease severity. Blue dots indicate admission and red dots show discharge; the duration of hospitalization is indicated at the bottom of each panel. Graphs show the correlation between anti-S-RBD IgM or IgG and neutralizing activity (C) and age, as well as the change in neutralizing activity against the WT strain in patients infected with the WT and VOCs (D). Each dot denotes disease severity, and Spearman’s correlation coefficients are shown within the graph. Gray areas indicate a 95% confidence interval (CI) for the total. (E) Dot plot indicates the neutralizing potency against the WT strain by gender. Graphs show the neutralizing activity against WT (F), Kappa (G), and Delta (H) variants at each severity (moderate II is classified by the infected strains). Statistical significances were determined using the Tukey’s test and Welch’s t-test. All horizontal bars show mean values. Cross, deceased; #, long-term observed healthcare worker. *p < 0.05; ***p < 0.005; ns, not significant.

    Article Snippet: Isolated PBMCs were incubated with His-tagged SARS-CoV-2 S-RBD recombinant protein (Cell Signaling Technology, Danvers, MA, USA) for 30 min on ice.

    Techniques: Activity Assay, Infection

    (A) Concentration of anti-S-RBD IgM and IgG in the serum of a healthcare worker (indicated by # in Figure 1) upon infection. (B) Fluctuation of each neutralizing activity from onset to post-vaccination. (C) Representative FACS plots of cell population are shown in panels. The numbers indicate the positive rate of each cell population. Graphs show the absolute number of IgG + S-RBD + Bmems (D) and the frequency of lymphocyte (E), CD19 + CD27 + CD38 high , and IgG + Bmems (F). Each number indicates the mean.

    Journal: medRxiv

    Article Title: Longitudinal analyses after COVID-19 recovery or prolonged infection reveal unique immunological signatures after repeated vaccinations

    doi: 10.1101/2022.08.03.22278353

    Figure Lengend Snippet: (A) Concentration of anti-S-RBD IgM and IgG in the serum of a healthcare worker (indicated by # in Figure 1) upon infection. (B) Fluctuation of each neutralizing activity from onset to post-vaccination. (C) Representative FACS plots of cell population are shown in panels. The numbers indicate the positive rate of each cell population. Graphs show the absolute number of IgG + S-RBD + Bmems (D) and the frequency of lymphocyte (E), CD19 + CD27 + CD38 high , and IgG + Bmems (F). Each number indicates the mean.

    Article Snippet: Isolated PBMCs were incubated with His-tagged SARS-CoV-2 S-RBD recombinant protein (Cell Signaling Technology, Danvers, MA, USA) for 30 min on ice.

    Techniques: Concentration Assay, Infection, Activity Assay

    Study approach and distinct antibody responses to SARS-CoV-2 along with neutralization assay. (A) Outline delineating the experimental workflow. (B) Box plots showing the anti-SARS-CoV-2 total, IgA, IgM, and IgG antibody proportions in serum samples from controls, symptomatic- and asymptomatic-infected individuals and in contacts (C) . Pie chart showing the overall immunoglobulin response across subject groups. (D) Box plot depicting the percent inhibition of neutralizing antibodies in an in vitro spike RBD-ACE2 interaction-based surrogate virus neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test. p-values are written against respective comparisons, where n = number of individuals; IgA, immunoglobulin A; IgM, immunoglobulin M; IgG, immunoglobulin G; control (n = 14), infected (n = 23), symptomatic (n = 10), asymptomatic (n = 13), and contact (n = 24).

    Journal: Frontiers in Immunology

    Article Title: Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases

    doi: 10.3389/fimmu.2021.733539

    Figure Lengend Snippet: Study approach and distinct antibody responses to SARS-CoV-2 along with neutralization assay. (A) Outline delineating the experimental workflow. (B) Box plots showing the anti-SARS-CoV-2 total, IgA, IgM, and IgG antibody proportions in serum samples from controls, symptomatic- and asymptomatic-infected individuals and in contacts (C) . Pie chart showing the overall immunoglobulin response across subject groups. (D) Box plot depicting the percent inhibition of neutralizing antibodies in an in vitro spike RBD-ACE2 interaction-based surrogate virus neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test. p-values are written against respective comparisons, where n = number of individuals; IgA, immunoglobulin A; IgM, immunoglobulin M; IgG, immunoglobulin G; control (n = 14), infected (n = 23), symptomatic (n = 10), asymptomatic (n = 13), and contact (n = 24).

    Article Snippet: For IgA and IgM quantification, receptor binding domain (RBD) (SARS-CoV-2 spike RBD recombinant protein, mFc-Tag, CST # 41701S) antigen at a concentration of 200 ng/well was coated in a 96-well high binding microtiter plate (HIMEDIA-EP1) in 1× Tris-buffered saline (TBS) pH-7.4 for 2 h at 37°C.

    Techniques: Neutralization, Infection, Inhibition, In Vitro

    A , Box plots showing the anti-SARS-CoV-2 IgA, IgM and IgG antibody proportions in serum samples from control, infected and contact along with B , Serum cytokine levels in pg/ml (Eotaxin, G-CSF, IL-7, MIF and MIP 1-□) determined by bio plex. C , The area under the receiver-operating characteristic (ROC) curve of the cytokines having more than 0.8 AUC. D , Box plot depicting the percent inhibition of neutralizing antibodies in an in -vitro spike RBD-ACE2 interaction based Surrogate Virus Neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test, p-values are written against respective comparisons. Where, n = number of individuals, IgA-Immunoglobulin A, IgM-Immunoglobulin M and IgG-Immunoglobulin G, control (n-14), infected (n-23, Symptomatic n-10 and Asymptomatic n-13) and contact (n-24).

    Journal: medRxiv

    Article Title: SARS-CoV-2 specific immune-signature in direct contacts of COVID-19 cases protect them from contracting disease: A Retrospective Study

    doi: 10.1101/2021.03.11.21253367

    Figure Lengend Snippet: A , Box plots showing the anti-SARS-CoV-2 IgA, IgM and IgG antibody proportions in serum samples from control, infected and contact along with B , Serum cytokine levels in pg/ml (Eotaxin, G-CSF, IL-7, MIF and MIP 1-□) determined by bio plex. C , The area under the receiver-operating characteristic (ROC) curve of the cytokines having more than 0.8 AUC. D , Box plot depicting the percent inhibition of neutralizing antibodies in an in -vitro spike RBD-ACE2 interaction based Surrogate Virus Neutralization assay. Statistical comparisons were performed using unpaired Wilcoxon test, p-values are written against respective comparisons. Where, n = number of individuals, IgA-Immunoglobulin A, IgM-Immunoglobulin M and IgG-Immunoglobulin G, control (n-14), infected (n-23, Symptomatic n-10 and Asymptomatic n-13) and contact (n-24).

    Article Snippet: For IgA and IgM quantification, RBD (SARS-CoV-2 spike RBD recombinant protein, mFc-Tag, CST # 41701S) antigen at a concentration of 200ng/well was coated in a 96-well high binding micro titre plate (HIMEDIA-EP1) in 1x TBS pH-7.4 for 2h at 37□.

    Techniques: Infection, Inhibition, In Vitro, Neutralization