hoechst 33 342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33 342
    Hoechst 33 342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hoechst 33 342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33 342
    Hoechst 33 342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dye hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc dye hoechst 33342
    Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with <t>Hoechst</t> <t>33342</t> (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.
    Dye Hoechst 33342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Zinc Oxide Nanoparticles Induce Necrosis and Apoptosis in Macrophages in a p47phox- and Nrf2-Independent Manner"

    Article Title: Zinc Oxide Nanoparticles Induce Necrosis and Apoptosis in Macrophages in a p47phox- and Nrf2-Independent Manner

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065704

    Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with Hoechst 33342 (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.
    Figure Legend Snippet: Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with Hoechst 33342 (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.

    Techniques Used: Staining

    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
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    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
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    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
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    hoechst33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst33342
    Hoechst33342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
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    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
    ( A ) Specific inhibition of mutant EGFR reporter alleles by ASP-RNAi. The effects of allele-specific siRNAs, si747/49_3D8 and si746/50_3D4, on expression of the target L747_E749del, A750P and E746_A750del EGFR mutant reporter alleles, respectively, and of the normal reporter alleles were examined using IC50 analysis (details in Methods). The IC50 values of the siRNAs for inhibition of the mutant and wild-type alleles are indicated (n=4, mean ± SDs). ( B ) Specific suppression of endogenous or oncogenic EGFR alleles. The si747/49_3D8 and si746/50_3D4 siRNAs were introduced into PC-3 and PC-9 human adenocarcinoma cells possessing the L747_E749del, A750P or E746_A750del mutations, respectively; endogenous EGFR mRNAs were examined using RT-PCR. Cells transfected with siControl (non-silencing siRNA) were studied as a control. M: DNA marker. ( C ) ASP-RNAi-mediated inhibition of PC-3 and PC-9 cell proliferation. Single siRNAs were transfected into PC-3 and PC-9 cells; subsequently, the cells were stained with <t>Hoechst</t> <t>33342</t> (blue) and propidium iodide (PI) (red) at the indicated time points, and examined using a fluorescent microscope. The numbers of total and dead cells were counted in four different 1-mm 2 areas. The data are averages of the four counts (± SDs; * P < 0.05). ( D ) Effect of allele-specific siRNAs on cell viability. Viability of PC-3 and PC-9 cells following treatment with the indicated siRNA was examined using the MTS assay (n=4, mean ± SDs).
    Hoechst 33342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference"

    Article Title: Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0073214

    ( A ) Specific inhibition of mutant EGFR reporter alleles by ASP-RNAi. The effects of allele-specific siRNAs, si747/49_3D8 and si746/50_3D4, on expression of the target L747_E749del, A750P and E746_A750del EGFR mutant reporter alleles, respectively, and of the normal reporter alleles were examined using IC50 analysis (details in Methods). The IC50 values of the siRNAs for inhibition of the mutant and wild-type alleles are indicated (n=4, mean ± SDs). ( B ) Specific suppression of endogenous or oncogenic EGFR alleles. The si747/49_3D8 and si746/50_3D4 siRNAs were introduced into PC-3 and PC-9 human adenocarcinoma cells possessing the L747_E749del, A750P or E746_A750del mutations, respectively; endogenous EGFR mRNAs were examined using RT-PCR. Cells transfected with siControl (non-silencing siRNA) were studied as a control. M: DNA marker. ( C ) ASP-RNAi-mediated inhibition of PC-3 and PC-9 cell proliferation. Single siRNAs were transfected into PC-3 and PC-9 cells; subsequently, the cells were stained with Hoechst 33342 (blue) and propidium iodide (PI) (red) at the indicated time points, and examined using a fluorescent microscope. The numbers of total and dead cells were counted in four different 1-mm 2 areas. The data are averages of the four counts (± SDs; * P < 0.05). ( D ) Effect of allele-specific siRNAs on cell viability. Viability of PC-3 and PC-9 cells following treatment with the indicated siRNA was examined using the MTS assay (n=4, mean ± SDs).
    Figure Legend Snippet: ( A ) Specific inhibition of mutant EGFR reporter alleles by ASP-RNAi. The effects of allele-specific siRNAs, si747/49_3D8 and si746/50_3D4, on expression of the target L747_E749del, A750P and E746_A750del EGFR mutant reporter alleles, respectively, and of the normal reporter alleles were examined using IC50 analysis (details in Methods). The IC50 values of the siRNAs for inhibition of the mutant and wild-type alleles are indicated (n=4, mean ± SDs). ( B ) Specific suppression of endogenous or oncogenic EGFR alleles. The si747/49_3D8 and si746/50_3D4 siRNAs were introduced into PC-3 and PC-9 human adenocarcinoma cells possessing the L747_E749del, A750P or E746_A750del mutations, respectively; endogenous EGFR mRNAs were examined using RT-PCR. Cells transfected with siControl (non-silencing siRNA) were studied as a control. M: DNA marker. ( C ) ASP-RNAi-mediated inhibition of PC-3 and PC-9 cell proliferation. Single siRNAs were transfected into PC-3 and PC-9 cells; subsequently, the cells were stained with Hoechst 33342 (blue) and propidium iodide (PI) (red) at the indicated time points, and examined using a fluorescent microscope. The numbers of total and dead cells were counted in four different 1-mm 2 areas. The data are averages of the four counts (± SDs; * P < 0.05). ( D ) Effect of allele-specific siRNAs on cell viability. Viability of PC-3 and PC-9 cells following treatment with the indicated siRNA was examined using the MTS assay (n=4, mean ± SDs).

    Techniques Used: Inhibition, Mutagenesis, Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Marker, Staining, Microscopy, MTS Assay

    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
    ( A ) Specific inhibition of mutant EGFR reporter alleles by ASP-RNAi. The effects of allele-specific siRNAs, si747/49_3D8 and si746/50_3D4, on expression of the target L747_E749del, A750P and E746_A750del EGFR mutant reporter alleles, respectively, and of the normal reporter alleles were examined using IC50 analysis (details in Methods). The IC50 values of the siRNAs for inhibition of the mutant and wild-type alleles are indicated (n=4, mean ± SDs). ( B ) Specific suppression of endogenous or oncogenic EGFR alleles. The si747/49_3D8 and si746/50_3D4 siRNAs were introduced into PC-3 and PC-9 human adenocarcinoma cells possessing the L747_E749del, A750P or E746_A750del mutations, respectively; endogenous EGFR mRNAs were examined using RT-PCR. Cells transfected with siControl (non-silencing siRNA) were studied as a control. M: DNA marker. ( C ) ASP-RNAi-mediated inhibition of PC-3 and PC-9 cell proliferation. Single siRNAs were transfected into PC-3 and PC-9 cells; subsequently, the cells were stained with <t>Hoechst</t> <t>33342</t> (blue) and propidium iodide (PI) (red) at the indicated time points, and examined using a fluorescent microscope. The numbers of total and dead cells were counted in four different 1-mm 2 areas. The data are averages of the four counts (± SDs; * P < 0.05). ( D ) Effect of allele-specific siRNAs on cell viability. Viability of PC-3 and PC-9 cells following treatment with the indicated siRNA was examined using the MTS assay (n=4, mean ± SDs).
    Hoechst 33342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference"

    Article Title: Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0073214

    ( A ) Specific inhibition of mutant EGFR reporter alleles by ASP-RNAi. The effects of allele-specific siRNAs, si747/49_3D8 and si746/50_3D4, on expression of the target L747_E749del, A750P and E746_A750del EGFR mutant reporter alleles, respectively, and of the normal reporter alleles were examined using IC50 analysis (details in Methods). The IC50 values of the siRNAs for inhibition of the mutant and wild-type alleles are indicated (n=4, mean ± SDs). ( B ) Specific suppression of endogenous or oncogenic EGFR alleles. The si747/49_3D8 and si746/50_3D4 siRNAs were introduced into PC-3 and PC-9 human adenocarcinoma cells possessing the L747_E749del, A750P or E746_A750del mutations, respectively; endogenous EGFR mRNAs were examined using RT-PCR. Cells transfected with siControl (non-silencing siRNA) were studied as a control. M: DNA marker. ( C ) ASP-RNAi-mediated inhibition of PC-3 and PC-9 cell proliferation. Single siRNAs were transfected into PC-3 and PC-9 cells; subsequently, the cells were stained with Hoechst 33342 (blue) and propidium iodide (PI) (red) at the indicated time points, and examined using a fluorescent microscope. The numbers of total and dead cells were counted in four different 1-mm 2 areas. The data are averages of the four counts (± SDs; * P < 0.05). ( D ) Effect of allele-specific siRNAs on cell viability. Viability of PC-3 and PC-9 cells following treatment with the indicated siRNA was examined using the MTS assay (n=4, mean ± SDs).
    Figure Legend Snippet: ( A ) Specific inhibition of mutant EGFR reporter alleles by ASP-RNAi. The effects of allele-specific siRNAs, si747/49_3D8 and si746/50_3D4, on expression of the target L747_E749del, A750P and E746_A750del EGFR mutant reporter alleles, respectively, and of the normal reporter alleles were examined using IC50 analysis (details in Methods). The IC50 values of the siRNAs for inhibition of the mutant and wild-type alleles are indicated (n=4, mean ± SDs). ( B ) Specific suppression of endogenous or oncogenic EGFR alleles. The si747/49_3D8 and si746/50_3D4 siRNAs were introduced into PC-3 and PC-9 human adenocarcinoma cells possessing the L747_E749del, A750P or E746_A750del mutations, respectively; endogenous EGFR mRNAs were examined using RT-PCR. Cells transfected with siControl (non-silencing siRNA) were studied as a control. M: DNA marker. ( C ) ASP-RNAi-mediated inhibition of PC-3 and PC-9 cell proliferation. Single siRNAs were transfected into PC-3 and PC-9 cells; subsequently, the cells were stained with Hoechst 33342 (blue) and propidium iodide (PI) (red) at the indicated time points, and examined using a fluorescent microscope. The numbers of total and dead cells were counted in four different 1-mm 2 areas. The data are averages of the four counts (± SDs; * P < 0.05). ( D ) Effect of allele-specific siRNAs on cell viability. Viability of PC-3 and PC-9 cells following treatment with the indicated siRNA was examined using the MTS assay (n=4, mean ± SDs).

    Techniques Used: Inhibition, Mutagenesis, Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Marker, Staining, Microscopy, MTS Assay

    hoechst 33342  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc hoechst 33342
    Hoechst 33342, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc hoechst 33 342
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    Cell Signaling Technology Inc dye hoechst 33342
    Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with <t>Hoechst</t> <t>33342</t> (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.
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    Cell Signaling Technology Inc hoechst 33342
    Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with <t>Hoechst</t> <t>33342</t> (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.
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    Cell Signaling Technology Inc hoechst33342
    Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with <t>Hoechst</t> <t>33342</t> (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.
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    Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with Hoechst 33342 (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.

    Journal: PLoS ONE

    Article Title: Zinc Oxide Nanoparticles Induce Necrosis and Apoptosis in Macrophages in a p47phox- and Nrf2-Independent Manner

    doi: 10.1371/journal.pone.0065704

    Figure Lengend Snippet: Representative images are shown for activated caspase-3 (first column) and corresponding DNA staining with Hoechst 33342 (second column). To control for unspecific staining, CC-3 antibody was substituted by IgG antibody (third column) and shown with the corresponding Hoechst 33342 staining (fourth column). Original magnification 400×.

    Article Snippet: Nuclear staining was performed by using the dye Hoechst 33342 (Cell Signaling) in a final concentration of 1 µg/ml in PBS for 15 min at 37°C in a humid box.

    Techniques: Staining