phospho akt ser473  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 - by Bioz Stars, 2023-02
    99/100 stars

    Images

    phospho akt ser473  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 - by Bioz Stars, 2023-02
    99/100 stars

    Images

    mouse monoclonal  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc mouse monoclonal
    Mouse Monoclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal - by Bioz Stars, 2023-02
    99/100 stars

    Images

    mouse monoclonal anti pakt ser473 antibody  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc mouse monoclonal anti pakt ser473 antibody
    Mouse Monoclonal Anti Pakt Ser473 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti pakt ser473 antibody/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti pakt ser473 antibody - by Bioz Stars, 2023-02
    99/100 stars

    Images

    akt phospho akt s473  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc akt phospho akt s473
    Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . <t>Akt</t> is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt <t>(S473))</t> were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.
    Akt Phospho Akt S473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt phospho akt s473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    akt phospho akt s473 - by Bioz Stars, 2023-02
    99/100 stars

    Images

    1) Product Images from "Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cells"

    Article Title: Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cells

    Journal: Breast Cancer Research : BCR

    doi: 10.1186/bcr2204

    Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . Akt is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt (S473)) were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.
    Figure Legend Snippet: Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . Akt is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt (S473)) were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.

    Techniques Used: Expressing, Protein Array, Activity Assay, Western Blot, Transformation Assay, MANN-WHITNEY

    Phosphatidylinositol 3-kinase (PI3K) and mTOR inhibitors inhibit basal-like cell line proliferation whereas apoptosis is induced only by PI3K inhibition . (a) Akt activation is associated with low/lack of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in human basal-like cell lines. The expression of Akt, phospho-Akt (S473) and PTEN were analysed by Western blotting in four basal-like (BT20, HCC38, HCC1937 and MDA-MB-468), one human epidermal growth factor receptor overexpressing (HER2+) (SKBr3) and one luminal (MDA-MB-453) human breast cell lines as well as in epidermal growth factor stimulated (EGF) or not (-) A431 cells. (b) PI3K and mTOR inhibition induce cell growth arrest of basal-like cell lines. BT20 (blue triangle), HCC1937 (red square) and MDA-MB-468 (green diamond) cells were exposed continuously for seven days to increasing concentrations of LY294002 (upper panel) or rapamycin (lower panel). Growth was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) dye conversion and presented as the percentage of control cell growth inhibition obtained from DMSO-treated cells. The x axes represent logarithmic transformed concentration of drugs. (c,d) The inhibition of PI3K, but not mTOR, induces apoptosis in basal-like cell lines. BT20, HCC1937 and MDA-MB-468 were exposed to varying concentrations of LY294002 (0 to 100 μM) or rapamycin (0 to 100 nM) for 24 hours and apoptosis was detected by measuring (c) caspase3/7 activity and the (d) cleavage of PARP (cPARP). (c) Caspase 3/7 activity was normalised by caspase 3/7 activity from vehicle-treated cells. (d) Actin was used as a loading control. The data represented the (b,c) average of three separate experiments performed in triplicates or are representative of (a,d) three separate experiments. Error bars represent standard deviation and p values (** p < 0.01, *** p < 0.001) were calculated by using Student's t test.
    Figure Legend Snippet: Phosphatidylinositol 3-kinase (PI3K) and mTOR inhibitors inhibit basal-like cell line proliferation whereas apoptosis is induced only by PI3K inhibition . (a) Akt activation is associated with low/lack of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in human basal-like cell lines. The expression of Akt, phospho-Akt (S473) and PTEN were analysed by Western blotting in four basal-like (BT20, HCC38, HCC1937 and MDA-MB-468), one human epidermal growth factor receptor overexpressing (HER2+) (SKBr3) and one luminal (MDA-MB-453) human breast cell lines as well as in epidermal growth factor stimulated (EGF) or not (-) A431 cells. (b) PI3K and mTOR inhibition induce cell growth arrest of basal-like cell lines. BT20 (blue triangle), HCC1937 (red square) and MDA-MB-468 (green diamond) cells were exposed continuously for seven days to increasing concentrations of LY294002 (upper panel) or rapamycin (lower panel). Growth was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) dye conversion and presented as the percentage of control cell growth inhibition obtained from DMSO-treated cells. The x axes represent logarithmic transformed concentration of drugs. (c,d) The inhibition of PI3K, but not mTOR, induces apoptosis in basal-like cell lines. BT20, HCC1937 and MDA-MB-468 were exposed to varying concentrations of LY294002 (0 to 100 μM) or rapamycin (0 to 100 nM) for 24 hours and apoptosis was detected by measuring (c) caspase3/7 activity and the (d) cleavage of PARP (cPARP). (c) Caspase 3/7 activity was normalised by caspase 3/7 activity from vehicle-treated cells. (d) Actin was used as a loading control. The data represented the (b,c) average of three separate experiments performed in triplicates or are representative of (a,d) three separate experiments. Error bars represent standard deviation and p values (** p < 0.01, *** p < 0.001) were calculated by using Student's t test.

    Techniques Used: Inhibition, Activation Assay, Expressing, Western Blot, Transformation Assay, Concentration Assay, Activity Assay, Standard Deviation

    phospho akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc phospho akt
    Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt - by Bioz Stars, 2023-02
    99/100 stars

    Images

    mouse anti phosphor akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc mouse anti phosphor akt
    Protein phosphorylation analyses of <t>Akt</t> <t>and</t> <t>AMPK</t> levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.
    Mouse Anti Phosphor Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti phosphor akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti phosphor akt - by Bioz Stars, 2023-02
    99/100 stars

    Images

    1) Product Images from "Hepatic Glucose Intolerance Precedes Hepatic Steatosis in the Male Aromatase Knockout (ArKO) Mouse"

    Article Title: Hepatic Glucose Intolerance Precedes Hepatic Steatosis in the Male Aromatase Knockout (ArKO) Mouse

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087230

    Protein phosphorylation analyses of Akt and AMPK levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.
    Figure Legend Snippet: Protein phosphorylation analyses of Akt and AMPK levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.

    Techniques Used: Knock-Out, Expressing

    anti p akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc anti p akt
    Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p akt - by Bioz Stars, 2023-02
    99/100 stars

    Images

    phospho akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc phospho akt
    HyFS induces autophagy through inhibition of the <t>AKT/mTOR</t> pathway. ( A ) A549 and H460 cells were treated with indicated concentrations of HyFS for 24 h. Cell lysates were used to detect Akt (S473), mTOR <t>(S2448),</t> <t>P70S6K</t> (S424/T421), and their phosphorylated counterparts by western blot. ( B ) Cells were treated with or without HyFS (0.5 μM) in combination with transfection by pCA-Akt plasmid or control vector for 24 h. Phosphorylated Akt (P-Akt), LC3-1, and LC3-II were detected by western blot. ( C ) Cells were treated with or without HyFS (0.5 μM) in combination with MK-2206 (an Akt inhibitor) for 24 h. Phosphorylated Akt (P-Akt), LC3-1, and LC3-II were detected by western blot. ( D ) Cells were treated with DMSO or HyFS (0.5 μM) in combination with or without transfection by pCA-Akt plasmid for 24 h. LC3 puncta and nuclei were observed by confocal microscopy. Data are expressed as mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001.
    Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt - by Bioz Stars, 2023-02
    99/100 stars

    Images

    1) Product Images from "3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells"

    Article Title: 3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells

    Journal: Theranostics

    doi: 10.7150/thno.23304

    HyFS induces autophagy through inhibition of the AKT/mTOR pathway. ( A ) A549 and H460 cells were treated with indicated concentrations of HyFS for 24 h. Cell lysates were used to detect Akt (S473), mTOR (S2448), P70S6K (S424/T421), and their phosphorylated counterparts by western blot. ( B ) Cells were treated with or without HyFS (0.5 μM) in combination with transfection by pCA-Akt plasmid or control vector for 24 h. Phosphorylated Akt (P-Akt), LC3-1, and LC3-II were detected by western blot. ( C ) Cells were treated with or without HyFS (0.5 μM) in combination with MK-2206 (an Akt inhibitor) for 24 h. Phosphorylated Akt (P-Akt), LC3-1, and LC3-II were detected by western blot. ( D ) Cells were treated with DMSO or HyFS (0.5 μM) in combination with or without transfection by pCA-Akt plasmid for 24 h. LC3 puncta and nuclei were observed by confocal microscopy. Data are expressed as mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: HyFS induces autophagy through inhibition of the AKT/mTOR pathway. ( A ) A549 and H460 cells were treated with indicated concentrations of HyFS for 24 h. Cell lysates were used to detect Akt (S473), mTOR (S2448), P70S6K (S424/T421), and their phosphorylated counterparts by western blot. ( B ) Cells were treated with or without HyFS (0.5 μM) in combination with transfection by pCA-Akt plasmid or control vector for 24 h. Phosphorylated Akt (P-Akt), LC3-1, and LC3-II were detected by western blot. ( C ) Cells were treated with or without HyFS (0.5 μM) in combination with MK-2206 (an Akt inhibitor) for 24 h. Phosphorylated Akt (P-Akt), LC3-1, and LC3-II were detected by western blot. ( D ) Cells were treated with DMSO or HyFS (0.5 μM) in combination with or without transfection by pCA-Akt plasmid for 24 h. LC3 puncta and nuclei were observed by confocal microscopy. Data are expressed as mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Inhibition, Western Blot, Transfection, Plasmid Preparation, Confocal Microscopy

    phospho akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc phospho akt
    Effect of oxygen tension <t>and</t> <t>IGF-1</t> or IGF-2 on signaling downstream kinases of cell surface receptors during osteogenic differentiation. Similar to Figures and , PMSCs were cultured for 14 days in osteogenic differentiation conditions containing 2% FBS in the presence or absence of 100 ng/mL of IGF-1 or IGF-2 in room air (20% O 2 ) or low oxygen levels (1% O 2 ). Treatments were stopped after 3, 7, and 14 days. Immunoblots, in Figure S3, were used to quantify protein levels of (a) p-ERK1/2, (b) <t>p-AKT,</t> and (c) p-RUNX2 over the three days. These kinases/phosphoproteins were normalized to their total kinase/phosphoprotein level and β -actin (two-way ANOVA, P < 0.05, N = 3). X indicates significance between different days without IGFs; # indicates significance of IGF addition compared with no IGFs in the same day; ∗ indicates significance between room air and low oxygen tension.
    Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt - by Bioz Stars, 2023-02
    99/100 stars

    Images

    1) Product Images from "Regulation of Osteogenic Differentiation of Placental-Derived Mesenchymal Stem Cells by Insulin-Like Growth Factors and Low Oxygen Tension"

    Article Title: Regulation of Osteogenic Differentiation of Placental-Derived Mesenchymal Stem Cells by Insulin-Like Growth Factors and Low Oxygen Tension

    Journal: Stem Cells International

    doi: 10.1155/2017/4576327

    Effect of oxygen tension and IGF-1 or IGF-2 on signaling downstream kinases of cell surface receptors during osteogenic differentiation. Similar to Figures and , PMSCs were cultured for 14 days in osteogenic differentiation conditions containing 2% FBS in the presence or absence of 100 ng/mL of IGF-1 or IGF-2 in room air (20% O 2 ) or low oxygen levels (1% O 2 ). Treatments were stopped after 3, 7, and 14 days. Immunoblots, in Figure S3, were used to quantify protein levels of (a) p-ERK1/2, (b) p-AKT, and (c) p-RUNX2 over the three days. These kinases/phosphoproteins were normalized to their total kinase/phosphoprotein level and β -actin (two-way ANOVA, P < 0.05, N = 3). X indicates significance between different days without IGFs; # indicates significance of IGF addition compared with no IGFs in the same day; ∗ indicates significance between room air and low oxygen tension.
    Figure Legend Snippet: Effect of oxygen tension and IGF-1 or IGF-2 on signaling downstream kinases of cell surface receptors during osteogenic differentiation. Similar to Figures and , PMSCs were cultured for 14 days in osteogenic differentiation conditions containing 2% FBS in the presence or absence of 100 ng/mL of IGF-1 or IGF-2 in room air (20% O 2 ) or low oxygen levels (1% O 2 ). Treatments were stopped after 3, 7, and 14 days. Immunoblots, in Figure S3, were used to quantify protein levels of (a) p-ERK1/2, (b) p-AKT, and (c) p-RUNX2 over the three days. These kinases/phosphoproteins were normalized to their total kinase/phosphoprotein level and β -actin (two-way ANOVA, P < 0.05, N = 3). X indicates significance between different days without IGFs; # indicates significance of IGF addition compared with no IGFs in the same day; ∗ indicates significance between room air and low oxygen tension.

    Techniques Used: Cell Culture, Western Blot

    total akt ser473  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc total akt ser473
    Total Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total akt ser473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total akt ser473 - by Bioz Stars, 2023-02
    99/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc mouse monoclonal
    Mouse Monoclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc mouse monoclonal anti pakt ser473 antibody
    Mouse Monoclonal Anti Pakt Ser473 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti pakt ser473 antibody/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti pakt ser473 antibody - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc akt phospho akt s473
    Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . <t>Akt</t> is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt <t>(S473))</t> were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.
    Akt Phospho Akt S473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt phospho akt s473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    akt phospho akt s473 - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc phospho akt
    Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . <t>Akt</t> is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt <t>(S473))</t> were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.
    Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc mouse anti phosphor akt
    Protein phosphorylation analyses of <t>Akt</t> <t>and</t> <t>AMPK</t> levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.
    Mouse Anti Phosphor Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti phosphor akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti phosphor akt - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc anti p akt
    Protein phosphorylation analyses of <t>Akt</t> <t>and</t> <t>AMPK</t> levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.
    Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p akt - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc total akt ser473
    Protein phosphorylation analyses of <t>Akt</t> <t>and</t> <t>AMPK</t> levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.
    Total Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total akt ser473/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total akt ser473 - by Bioz Stars, 2023-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . Akt is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt (S473)) were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.

    Journal: Breast Cancer Research : BCR

    Article Title: Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cells

    doi: 10.1186/bcr2204

    Figure Lengend Snippet: Up-regulated phosphatidylinositol 3-kinase (PI3K) signalling pathway in human basal-like breast cancers . Akt is activated in basal-like carcinomas (BLCs). The expression of (a) total Akt and (b) phosphorylated/active form of Akt (phospho-Akt (S473)) were measured by reverse phase protein array (RPPA) as well as Akt activity determined as the (c) ratio 'phospho/total' in human BLCs and human epidermal growth factor receptor overexpressing (HER2+) carcinomas. mTOR is activated in BLCs. Box plots show the (d) expression of mTOR and (e) its form phosphorylated via the PI3 kinase/Akt signalling pathway (phospho-mTOR (S2448)) determined by Western blotting as well as (f) mTOR activity (phospho/total ratio) in human BLCs and HER2+ carcinomas. Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). The y axes represent logarithmic transformed relative quantifications. p values (* p < 0.05) are represented (Mann-Whitney test). Data are representative of four and two separate experiments for RPPA and Western-blot, respectively.

    Article Snippet: Akt phospho-Akt (S473), PTEN and stathmin antibodies (Cell Signaling Technology, Ozyme, Saint Quentin en Yveline, France) were utilised at a dilution of 1:1000, 1:250, 1:200 and 1:100, respectively.

    Techniques: Expressing, Protein Array, Activity Assay, Western Blot, Transformation Assay, MANN-WHITNEY

    Phosphatidylinositol 3-kinase (PI3K) and mTOR inhibitors inhibit basal-like cell line proliferation whereas apoptosis is induced only by PI3K inhibition . (a) Akt activation is associated with low/lack of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in human basal-like cell lines. The expression of Akt, phospho-Akt (S473) and PTEN were analysed by Western blotting in four basal-like (BT20, HCC38, HCC1937 and MDA-MB-468), one human epidermal growth factor receptor overexpressing (HER2+) (SKBr3) and one luminal (MDA-MB-453) human breast cell lines as well as in epidermal growth factor stimulated (EGF) or not (-) A431 cells. (b) PI3K and mTOR inhibition induce cell growth arrest of basal-like cell lines. BT20 (blue triangle), HCC1937 (red square) and MDA-MB-468 (green diamond) cells were exposed continuously for seven days to increasing concentrations of LY294002 (upper panel) or rapamycin (lower panel). Growth was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) dye conversion and presented as the percentage of control cell growth inhibition obtained from DMSO-treated cells. The x axes represent logarithmic transformed concentration of drugs. (c,d) The inhibition of PI3K, but not mTOR, induces apoptosis in basal-like cell lines. BT20, HCC1937 and MDA-MB-468 were exposed to varying concentrations of LY294002 (0 to 100 μM) or rapamycin (0 to 100 nM) for 24 hours and apoptosis was detected by measuring (c) caspase3/7 activity and the (d) cleavage of PARP (cPARP). (c) Caspase 3/7 activity was normalised by caspase 3/7 activity from vehicle-treated cells. (d) Actin was used as a loading control. The data represented the (b,c) average of three separate experiments performed in triplicates or are representative of (a,d) three separate experiments. Error bars represent standard deviation and p values (** p < 0.01, *** p < 0.001) were calculated by using Student's t test.

    Journal: Breast Cancer Research : BCR

    Article Title: Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cells

    doi: 10.1186/bcr2204

    Figure Lengend Snippet: Phosphatidylinositol 3-kinase (PI3K) and mTOR inhibitors inhibit basal-like cell line proliferation whereas apoptosis is induced only by PI3K inhibition . (a) Akt activation is associated with low/lack of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in human basal-like cell lines. The expression of Akt, phospho-Akt (S473) and PTEN were analysed by Western blotting in four basal-like (BT20, HCC38, HCC1937 and MDA-MB-468), one human epidermal growth factor receptor overexpressing (HER2+) (SKBr3) and one luminal (MDA-MB-453) human breast cell lines as well as in epidermal growth factor stimulated (EGF) or not (-) A431 cells. (b) PI3K and mTOR inhibition induce cell growth arrest of basal-like cell lines. BT20 (blue triangle), HCC1937 (red square) and MDA-MB-468 (green diamond) cells were exposed continuously for seven days to increasing concentrations of LY294002 (upper panel) or rapamycin (lower panel). Growth was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) dye conversion and presented as the percentage of control cell growth inhibition obtained from DMSO-treated cells. The x axes represent logarithmic transformed concentration of drugs. (c,d) The inhibition of PI3K, but not mTOR, induces apoptosis in basal-like cell lines. BT20, HCC1937 and MDA-MB-468 were exposed to varying concentrations of LY294002 (0 to 100 μM) or rapamycin (0 to 100 nM) for 24 hours and apoptosis was detected by measuring (c) caspase3/7 activity and the (d) cleavage of PARP (cPARP). (c) Caspase 3/7 activity was normalised by caspase 3/7 activity from vehicle-treated cells. (d) Actin was used as a loading control. The data represented the (b,c) average of three separate experiments performed in triplicates or are representative of (a,d) three separate experiments. Error bars represent standard deviation and p values (** p < 0.01, *** p < 0.001) were calculated by using Student's t test.

    Article Snippet: Akt phospho-Akt (S473), PTEN and stathmin antibodies (Cell Signaling Technology, Ozyme, Saint Quentin en Yveline, France) were utilised at a dilution of 1:1000, 1:250, 1:200 and 1:100, respectively.

    Techniques: Inhibition, Activation Assay, Expressing, Western Blot, Transformation Assay, Concentration Assay, Activity Assay, Standard Deviation

    Protein phosphorylation analyses of Akt and AMPK levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.

    Journal: PLoS ONE

    Article Title: Hepatic Glucose Intolerance Precedes Hepatic Steatosis in the Male Aromatase Knockout (ArKO) Mouse

    doi: 10.1371/journal.pone.0087230

    Figure Lengend Snippet: Protein phosphorylation analyses of Akt and AMPK levels were performed on protein extracted from insulin stimulated ( a ) gonadal adipose tissue, ( b ) gastrocnemius muscle, ( c ) liver in insulin stimulated liver of 6 month-old male wildtype (WT) and aromatase knockout (KO) and 2.5 µg/day estrogen-replaced KO (KOE) mice. Expression data from 6–8 samples per genotype are shown, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus expression in age-matched WT samples and # p<0.05, ### p<0.001 versus KO samples.

    Article Snippet: Primary antibodies diluted in 1% BSA in Tris Buffered Saline with 1% Tween were incubated overnight at 4°C (mouse anti-Phosphor-Akt (Ser473), rabbit anti-Akt, rabbit anti-phosphor-AMPK (Thr172), rabbit anti-AMP alpha (Cell Signaling Technologies, Beverly MA, USA).

    Techniques: Knock-Out, Expressing