α mouse ace2  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc α mouse ace2
    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine <t>ACE2</t> protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.
    α Mouse Ace2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α mouse ace2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α mouse ace2 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes"

    Article Title: SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

    Journal: bioRxiv

    doi: 10.1101/2022.08.05.502936

    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine ACE2 protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.
    Figure Legend Snippet: (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine ACE2 protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.

    Techniques Used: Expressing, Western Blot, Infection, Immunofluorescence, Staining

    ( A and B ) Inhibition of B.1 entry. ( A ) Intracellular N gene RNA copies and ( B ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). Baf A1: bafilomycin A1. ( C and D ) Inhibition of B.1.617 entry. ( C ) Intracellular N gene RNA copies and ( D ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). ( E and F ) Environmental pH does not mediate fusion peptide exposure. ( E ) Intracellular N gene RNA copies and ( F ) B.1 secretion in the indicated cell-lines after infection at the stated pH (MOI=0.01, 24 hpi, n=3 ± SEM). Endogenous and ectopic ACE2 expression in A549 and Huh-7.5.1 cells. ( G ) Human ACE2 protein (left) and RNA transcript levels (right) in the indicated cell-lines. ( H ) Human TMPRSS2 protein (left) and transcript levels (right) in the indicated cell-lines. ( I ) Innate immunity correlates with impaired viral replication rates. The indicated cell-lines were infected with B.1 at an MOI of 0.01. Left: intracellular N gene RNA at 6 hpi. Right: relative fold change in vRNA levels at 24 hpi compared to 6 hpi (n=4 ± SEM). ( J ) Virion secretion is enhanced in the absence of innate immunity. Virion secretion at 24 hpi in the cells from ( I ). ( K ) In contrast to A549 cells, Huh7.5.1 cells do not upregulate IFN or ISGs upon Poly(I:C) stimulation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n.s.: no significance. n.d.: not detected.
    Figure Legend Snippet: ( A and B ) Inhibition of B.1 entry. ( A ) Intracellular N gene RNA copies and ( B ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). Baf A1: bafilomycin A1. ( C and D ) Inhibition of B.1.617 entry. ( C ) Intracellular N gene RNA copies and ( D ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). ( E and F ) Environmental pH does not mediate fusion peptide exposure. ( E ) Intracellular N gene RNA copies and ( F ) B.1 secretion in the indicated cell-lines after infection at the stated pH (MOI=0.01, 24 hpi, n=3 ± SEM). Endogenous and ectopic ACE2 expression in A549 and Huh-7.5.1 cells. ( G ) Human ACE2 protein (left) and RNA transcript levels (right) in the indicated cell-lines. ( H ) Human TMPRSS2 protein (left) and transcript levels (right) in the indicated cell-lines. ( I ) Innate immunity correlates with impaired viral replication rates. The indicated cell-lines were infected with B.1 at an MOI of 0.01. Left: intracellular N gene RNA at 6 hpi. Right: relative fold change in vRNA levels at 24 hpi compared to 6 hpi (n=4 ± SEM). ( J ) Virion secretion is enhanced in the absence of innate immunity. Virion secretion at 24 hpi in the cells from ( I ). ( K ) In contrast to A549 cells, Huh7.5.1 cells do not upregulate IFN or ISGs upon Poly(I:C) stimulation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n.s.: no significance. n.d.: not detected.

    Techniques Used: Inhibition, Infection, Expressing

    Intrinsic and inducible gene expression in parental and engineered A549 cells. ( A ) Transcriptional profiling of parental and engineered A549 cells without infection (n=3). Heat map visualizes intrinsic mRNA expression of viral entry factors, IFN receptors, dsRNA pattern recognition receptors (PRRs) and antiviral transcription factors (TFs) in the indicated cell-lines. Absent or minimal endogenous ACE2 and TMPRSS2 expression mRNAs is confirmed in parental A549 cells, while abundant ectopic expression is confirmed in engineered cells. ( B ) Robust and broad IFN induction after Poly(I:C) treatment of parental A549 cells (n=3, mean expression presented). Lentiviral gene transfer of ACE2 and TMPRSS2 does not induce IFNs. NRCs: normalized read counts.
    Figure Legend Snippet: Intrinsic and inducible gene expression in parental and engineered A549 cells. ( A ) Transcriptional profiling of parental and engineered A549 cells without infection (n=3). Heat map visualizes intrinsic mRNA expression of viral entry factors, IFN receptors, dsRNA pattern recognition receptors (PRRs) and antiviral transcription factors (TFs) in the indicated cell-lines. Absent or minimal endogenous ACE2 and TMPRSS2 expression mRNAs is confirmed in parental A549 cells, while abundant ectopic expression is confirmed in engineered cells. ( B ) Robust and broad IFN induction after Poly(I:C) treatment of parental A549 cells (n=3, mean expression presented). Lentiviral gene transfer of ACE2 and TMPRSS2 does not induce IFNs. NRCs: normalized read counts.

    Techniques Used: Expressing, Infection

    (A) Phylogenetic tree depicts evolutionary relationships of TMPRSS2 coding sequences. Branch length are proportional nucleotide substitutions per site, as determined by the scale bar. Species selected for further experimental investigation are highlighted. ( B ) Conservation of serine protease catalytic triad residues in humans and selected nonhuman species. ( C ) Hydrophobicity plots identify a single putative transmembrane domain in humans and nonhuman species. ( D ) Viron production rates at 24 hpi in A549-A cells co-expressing the indicated human and nonhuman TPMRSS2 orthologues. ( E ) SARS-CoV-2 pseudotyped vector transduction rates in HEK-293-ACE2 cells expressing indicated human and nonhuman TPMRSS2 orthologues. ( F-H ) TMPRSS2 structural predictions. ( F ) Human TMPRSS2 prediction with TM domain (blue) and HDS catalytic triad residues (red) highlighted. ( G ) Amino acid variability in mammalian TMPRSS2 orthologues superimposed onto the human TMPRSS2 structural prediction. ( H ) Amino acid differences (red) and conservation (blue) between mammalian and zebrafish TMPRSS2 orthologues are superimposed onto the zebrafish TMPRSS2 structural prediction.
    Figure Legend Snippet: (A) Phylogenetic tree depicts evolutionary relationships of TMPRSS2 coding sequences. Branch length are proportional nucleotide substitutions per site, as determined by the scale bar. Species selected for further experimental investigation are highlighted. ( B ) Conservation of serine protease catalytic triad residues in humans and selected nonhuman species. ( C ) Hydrophobicity plots identify a single putative transmembrane domain in humans and nonhuman species. ( D ) Viron production rates at 24 hpi in A549-A cells co-expressing the indicated human and nonhuman TPMRSS2 orthologues. ( E ) SARS-CoV-2 pseudotyped vector transduction rates in HEK-293-ACE2 cells expressing indicated human and nonhuman TPMRSS2 orthologues. ( F-H ) TMPRSS2 structural predictions. ( F ) Human TMPRSS2 prediction with TM domain (blue) and HDS catalytic triad residues (red) highlighted. ( G ) Amino acid variability in mammalian TMPRSS2 orthologues superimposed onto the human TMPRSS2 structural prediction. ( H ) Amino acid differences (red) and conservation (blue) between mammalian and zebrafish TMPRSS2 orthologues are superimposed onto the zebrafish TMPRSS2 structural prediction.

    Techniques Used: Expressing, Plasmid Preparation, Transduction

    α mouse ace2  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc α mouse ace2
    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine <t>ACE2</t> protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.
    α Mouse Ace2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α mouse ace2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α mouse ace2 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes"

    Article Title: SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

    Journal: bioRxiv

    doi: 10.1101/2022.08.05.502936

    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine ACE2 protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.
    Figure Legend Snippet: (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine ACE2 protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.

    Techniques Used: Expressing, Western Blot, Infection, Immunofluorescence, Staining

    ( A and B ) Inhibition of B.1 entry. ( A ) Intracellular N gene RNA copies and ( B ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). Baf A1: bafilomycin A1. ( C and D ) Inhibition of B.1.617 entry. ( C ) Intracellular N gene RNA copies and ( D ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). ( E and F ) Environmental pH does not mediate fusion peptide exposure. ( E ) Intracellular N gene RNA copies and ( F ) B.1 secretion in the indicated cell-lines after infection at the stated pH (MOI=0.01, 24 hpi, n=3 ± SEM). Endogenous and ectopic ACE2 expression in A549 and Huh-7.5.1 cells. ( G ) Human ACE2 protein (left) and RNA transcript levels (right) in the indicated cell-lines. ( H ) Human TMPRSS2 protein (left) and transcript levels (right) in the indicated cell-lines. ( I ) Innate immunity correlates with impaired viral replication rates. The indicated cell-lines were infected with B.1 at an MOI of 0.01. Left: intracellular N gene RNA at 6 hpi. Right: relative fold change in vRNA levels at 24 hpi compared to 6 hpi (n=4 ± SEM). ( J ) Virion secretion is enhanced in the absence of innate immunity. Virion secretion at 24 hpi in the cells from ( I ). ( K ) In contrast to A549 cells, Huh7.5.1 cells do not upregulate IFN or ISGs upon Poly(I:C) stimulation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n.s.: no significance. n.d.: not detected.
    Figure Legend Snippet: ( A and B ) Inhibition of B.1 entry. ( A ) Intracellular N gene RNA copies and ( B ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). Baf A1: bafilomycin A1. ( C and D ) Inhibition of B.1.617 entry. ( C ) Intracellular N gene RNA copies and ( D ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). ( E and F ) Environmental pH does not mediate fusion peptide exposure. ( E ) Intracellular N gene RNA copies and ( F ) B.1 secretion in the indicated cell-lines after infection at the stated pH (MOI=0.01, 24 hpi, n=3 ± SEM). Endogenous and ectopic ACE2 expression in A549 and Huh-7.5.1 cells. ( G ) Human ACE2 protein (left) and RNA transcript levels (right) in the indicated cell-lines. ( H ) Human TMPRSS2 protein (left) and transcript levels (right) in the indicated cell-lines. ( I ) Innate immunity correlates with impaired viral replication rates. The indicated cell-lines were infected with B.1 at an MOI of 0.01. Left: intracellular N gene RNA at 6 hpi. Right: relative fold change in vRNA levels at 24 hpi compared to 6 hpi (n=4 ± SEM). ( J ) Virion secretion is enhanced in the absence of innate immunity. Virion secretion at 24 hpi in the cells from ( I ). ( K ) In contrast to A549 cells, Huh7.5.1 cells do not upregulate IFN or ISGs upon Poly(I:C) stimulation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n.s.: no significance. n.d.: not detected.

    Techniques Used: Inhibition, Infection, Expressing

    Intrinsic and inducible gene expression in parental and engineered A549 cells. ( A ) Transcriptional profiling of parental and engineered A549 cells without infection (n=3). Heat map visualizes intrinsic mRNA expression of viral entry factors, IFN receptors, dsRNA pattern recognition receptors (PRRs) and antiviral transcription factors (TFs) in the indicated cell-lines. Absent or minimal endogenous ACE2 and TMPRSS2 expression mRNAs is confirmed in parental A549 cells, while abundant ectopic expression is confirmed in engineered cells. ( B ) Robust and broad IFN induction after Poly(I:C) treatment of parental A549 cells (n=3, mean expression presented). Lentiviral gene transfer of ACE2 and TMPRSS2 does not induce IFNs. NRCs: normalized read counts.
    Figure Legend Snippet: Intrinsic and inducible gene expression in parental and engineered A549 cells. ( A ) Transcriptional profiling of parental and engineered A549 cells without infection (n=3). Heat map visualizes intrinsic mRNA expression of viral entry factors, IFN receptors, dsRNA pattern recognition receptors (PRRs) and antiviral transcription factors (TFs) in the indicated cell-lines. Absent or minimal endogenous ACE2 and TMPRSS2 expression mRNAs is confirmed in parental A549 cells, while abundant ectopic expression is confirmed in engineered cells. ( B ) Robust and broad IFN induction after Poly(I:C) treatment of parental A549 cells (n=3, mean expression presented). Lentiviral gene transfer of ACE2 and TMPRSS2 does not induce IFNs. NRCs: normalized read counts.

    Techniques Used: Expressing, Infection

    (A) Phylogenetic tree depicts evolutionary relationships of TMPRSS2 coding sequences. Branch length are proportional nucleotide substitutions per site, as determined by the scale bar. Species selected for further experimental investigation are highlighted. ( B ) Conservation of serine protease catalytic triad residues in humans and selected nonhuman species. ( C ) Hydrophobicity plots identify a single putative transmembrane domain in humans and nonhuman species. ( D ) Viron production rates at 24 hpi in A549-A cells co-expressing the indicated human and nonhuman TPMRSS2 orthologues. ( E ) SARS-CoV-2 pseudotyped vector transduction rates in HEK-293-ACE2 cells expressing indicated human and nonhuman TPMRSS2 orthologues. ( F-H ) TMPRSS2 structural predictions. ( F ) Human TMPRSS2 prediction with TM domain (blue) and HDS catalytic triad residues (red) highlighted. ( G ) Amino acid variability in mammalian TMPRSS2 orthologues superimposed onto the human TMPRSS2 structural prediction. ( H ) Amino acid differences (red) and conservation (blue) between mammalian and zebrafish TMPRSS2 orthologues are superimposed onto the zebrafish TMPRSS2 structural prediction.
    Figure Legend Snippet: (A) Phylogenetic tree depicts evolutionary relationships of TMPRSS2 coding sequences. Branch length are proportional nucleotide substitutions per site, as determined by the scale bar. Species selected for further experimental investigation are highlighted. ( B ) Conservation of serine protease catalytic triad residues in humans and selected nonhuman species. ( C ) Hydrophobicity plots identify a single putative transmembrane domain in humans and nonhuman species. ( D ) Viron production rates at 24 hpi in A549-A cells co-expressing the indicated human and nonhuman TPMRSS2 orthologues. ( E ) SARS-CoV-2 pseudotyped vector transduction rates in HEK-293-ACE2 cells expressing indicated human and nonhuman TPMRSS2 orthologues. ( F-H ) TMPRSS2 structural predictions. ( F ) Human TMPRSS2 prediction with TM domain (blue) and HDS catalytic triad residues (red) highlighted. ( G ) Amino acid variability in mammalian TMPRSS2 orthologues superimposed onto the human TMPRSS2 structural prediction. ( H ) Amino acid differences (red) and conservation (blue) between mammalian and zebrafish TMPRSS2 orthologues are superimposed onto the zebrafish TMPRSS2 structural prediction.

    Techniques Used: Expressing, Plasmid Preparation, Transduction

    tri methyl  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc tri methyl
    Tri Methyl, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri methyl/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tri methyl - by Bioz Stars, 2023-01
    94/100 stars

    Images

    tri methyl  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc tri methyl
    Tri Methyl, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri methyl/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tri methyl - by Bioz Stars, 2023-01
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Cell Signaling Technology Inc α mouse ace2
    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine <t>ACE2</t> protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.
    α Mouse Ace2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α mouse ace2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α mouse ace2 - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc tri methyl
    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine <t>ACE2</t> protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.
    Tri Methyl, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri methyl/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tri methyl - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine ACE2 protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

    doi: 10.1101/2022.08.05.502936

    Figure Lengend Snippet: (A ) Cartoon of human TMPRSS2 domains. Location of catalytic triad residues and auto-cleavage site (scissors) are highlighted above. ( B ) Human or murine ACE2 protein expression in the indicated cell lines, determined by Western blot analysis. ( C ) Human TMPRSS2 protein expression levels in the indicated cell lines, determined by Western blot analysis. Bands representing full-length protein and two fragments formed by auto-cleavage are highlighted. ( D ) Susceptibility of indicated cell lines to B.1, B.1.617 and B.1.1.529 infection (MOI=0.01, 24 hpi). Immunofluorescence staining of SARS-CoV-2 nucleocapsid protein (green) or cellular nuclei (blue). Scale bar: 200 μM. ( E ) Human TMPRSS2 enhances replication and transcription of B.1 and B.1.1.529 but not B.1.617. Intracellular N copy numbers per 10ng total RNA (MOI=0.01, 24 hpi, n=3 ± SEM). ( F ) Human TMPRSS2 increases the secretion rates in a strain dependent manner. Left panel: Viral titers in the medium of A549-AT and A549-AT(ΔHDS) cells at 24 hpi were determined using TCID 50 (n=6 ± SEM). Right panel: Virion secretion of the indicated strains from VeroE6 cells (MOI=0.01, 24 hpi, n=2 ± SEM). ****P<0.0001, ***P<0.001, **P<0.01, n.s.: no significance. n.d.: not detected.

    Article Snippet: The membrane was incubated with α-mouse ACE2 (cross-reactive to human ACE2) (1:1,000; #38241 Cell Signaling Technology), α-human TMPRSS2 (1:1,000; HPA035787 Sigma-Aldrich) or α-β-actin (1:1,000; Abcam) with a gentle agitation overnight at 4°C.

    Techniques: Expressing, Western Blot, Infection, Immunofluorescence, Staining

    ( A and B ) Inhibition of B.1 entry. ( A ) Intracellular N gene RNA copies and ( B ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). Baf A1: bafilomycin A1. ( C and D ) Inhibition of B.1.617 entry. ( C ) Intracellular N gene RNA copies and ( D ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). ( E and F ) Environmental pH does not mediate fusion peptide exposure. ( E ) Intracellular N gene RNA copies and ( F ) B.1 secretion in the indicated cell-lines after infection at the stated pH (MOI=0.01, 24 hpi, n=3 ± SEM). Endogenous and ectopic ACE2 expression in A549 and Huh-7.5.1 cells. ( G ) Human ACE2 protein (left) and RNA transcript levels (right) in the indicated cell-lines. ( H ) Human TMPRSS2 protein (left) and transcript levels (right) in the indicated cell-lines. ( I ) Innate immunity correlates with impaired viral replication rates. The indicated cell-lines were infected with B.1 at an MOI of 0.01. Left: intracellular N gene RNA at 6 hpi. Right: relative fold change in vRNA levels at 24 hpi compared to 6 hpi (n=4 ± SEM). ( J ) Virion secretion is enhanced in the absence of innate immunity. Virion secretion at 24 hpi in the cells from ( I ). ( K ) In contrast to A549 cells, Huh7.5.1 cells do not upregulate IFN or ISGs upon Poly(I:C) stimulation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n.s.: no significance. n.d.: not detected.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

    doi: 10.1101/2022.08.05.502936

    Figure Lengend Snippet: ( A and B ) Inhibition of B.1 entry. ( A ) Intracellular N gene RNA copies and ( B ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). Baf A1: bafilomycin A1. ( C and D ) Inhibition of B.1.617 entry. ( C ) Intracellular N gene RNA copies and ( D ) virion secretion in the indicated cell-lines after infection in the presence of inhibitors (MOI=0.01, 24 hpi, n=3 ± SEM). ( E and F ) Environmental pH does not mediate fusion peptide exposure. ( E ) Intracellular N gene RNA copies and ( F ) B.1 secretion in the indicated cell-lines after infection at the stated pH (MOI=0.01, 24 hpi, n=3 ± SEM). Endogenous and ectopic ACE2 expression in A549 and Huh-7.5.1 cells. ( G ) Human ACE2 protein (left) and RNA transcript levels (right) in the indicated cell-lines. ( H ) Human TMPRSS2 protein (left) and transcript levels (right) in the indicated cell-lines. ( I ) Innate immunity correlates with impaired viral replication rates. The indicated cell-lines were infected with B.1 at an MOI of 0.01. Left: intracellular N gene RNA at 6 hpi. Right: relative fold change in vRNA levels at 24 hpi compared to 6 hpi (n=4 ± SEM). ( J ) Virion secretion is enhanced in the absence of innate immunity. Virion secretion at 24 hpi in the cells from ( I ). ( K ) In contrast to A549 cells, Huh7.5.1 cells do not upregulate IFN or ISGs upon Poly(I:C) stimulation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n.s.: no significance. n.d.: not detected.

    Article Snippet: The membrane was incubated with α-mouse ACE2 (cross-reactive to human ACE2) (1:1,000; #38241 Cell Signaling Technology), α-human TMPRSS2 (1:1,000; HPA035787 Sigma-Aldrich) or α-β-actin (1:1,000; Abcam) with a gentle agitation overnight at 4°C.

    Techniques: Inhibition, Infection, Expressing

    Intrinsic and inducible gene expression in parental and engineered A549 cells. ( A ) Transcriptional profiling of parental and engineered A549 cells without infection (n=3). Heat map visualizes intrinsic mRNA expression of viral entry factors, IFN receptors, dsRNA pattern recognition receptors (PRRs) and antiviral transcription factors (TFs) in the indicated cell-lines. Absent or minimal endogenous ACE2 and TMPRSS2 expression mRNAs is confirmed in parental A549 cells, while abundant ectopic expression is confirmed in engineered cells. ( B ) Robust and broad IFN induction after Poly(I:C) treatment of parental A549 cells (n=3, mean expression presented). Lentiviral gene transfer of ACE2 and TMPRSS2 does not induce IFNs. NRCs: normalized read counts.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

    doi: 10.1101/2022.08.05.502936

    Figure Lengend Snippet: Intrinsic and inducible gene expression in parental and engineered A549 cells. ( A ) Transcriptional profiling of parental and engineered A549 cells without infection (n=3). Heat map visualizes intrinsic mRNA expression of viral entry factors, IFN receptors, dsRNA pattern recognition receptors (PRRs) and antiviral transcription factors (TFs) in the indicated cell-lines. Absent or minimal endogenous ACE2 and TMPRSS2 expression mRNAs is confirmed in parental A549 cells, while abundant ectopic expression is confirmed in engineered cells. ( B ) Robust and broad IFN induction after Poly(I:C) treatment of parental A549 cells (n=3, mean expression presented). Lentiviral gene transfer of ACE2 and TMPRSS2 does not induce IFNs. NRCs: normalized read counts.

    Article Snippet: The membrane was incubated with α-mouse ACE2 (cross-reactive to human ACE2) (1:1,000; #38241 Cell Signaling Technology), α-human TMPRSS2 (1:1,000; HPA035787 Sigma-Aldrich) or α-β-actin (1:1,000; Abcam) with a gentle agitation overnight at 4°C.

    Techniques: Expressing, Infection

    (A) Phylogenetic tree depicts evolutionary relationships of TMPRSS2 coding sequences. Branch length are proportional nucleotide substitutions per site, as determined by the scale bar. Species selected for further experimental investigation are highlighted. ( B ) Conservation of serine protease catalytic triad residues in humans and selected nonhuman species. ( C ) Hydrophobicity plots identify a single putative transmembrane domain in humans and nonhuman species. ( D ) Viron production rates at 24 hpi in A549-A cells co-expressing the indicated human and nonhuman TPMRSS2 orthologues. ( E ) SARS-CoV-2 pseudotyped vector transduction rates in HEK-293-ACE2 cells expressing indicated human and nonhuman TPMRSS2 orthologues. ( F-H ) TMPRSS2 structural predictions. ( F ) Human TMPRSS2 prediction with TM domain (blue) and HDS catalytic triad residues (red) highlighted. ( G ) Amino acid variability in mammalian TMPRSS2 orthologues superimposed onto the human TMPRSS2 structural prediction. ( H ) Amino acid differences (red) and conservation (blue) between mammalian and zebrafish TMPRSS2 orthologues are superimposed onto the zebrafish TMPRSS2 structural prediction.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

    doi: 10.1101/2022.08.05.502936

    Figure Lengend Snippet: (A) Phylogenetic tree depicts evolutionary relationships of TMPRSS2 coding sequences. Branch length are proportional nucleotide substitutions per site, as determined by the scale bar. Species selected for further experimental investigation are highlighted. ( B ) Conservation of serine protease catalytic triad residues in humans and selected nonhuman species. ( C ) Hydrophobicity plots identify a single putative transmembrane domain in humans and nonhuman species. ( D ) Viron production rates at 24 hpi in A549-A cells co-expressing the indicated human and nonhuman TPMRSS2 orthologues. ( E ) SARS-CoV-2 pseudotyped vector transduction rates in HEK-293-ACE2 cells expressing indicated human and nonhuman TPMRSS2 orthologues. ( F-H ) TMPRSS2 structural predictions. ( F ) Human TMPRSS2 prediction with TM domain (blue) and HDS catalytic triad residues (red) highlighted. ( G ) Amino acid variability in mammalian TMPRSS2 orthologues superimposed onto the human TMPRSS2 structural prediction. ( H ) Amino acid differences (red) and conservation (blue) between mammalian and zebrafish TMPRSS2 orthologues are superimposed onto the zebrafish TMPRSS2 structural prediction.

    Article Snippet: The membrane was incubated with α-mouse ACE2 (cross-reactive to human ACE2) (1:1,000; #38241 Cell Signaling Technology), α-human TMPRSS2 (1:1,000; HPA035787 Sigma-Aldrich) or α-β-actin (1:1,000; Abcam) with a gentle agitation overnight at 4°C.

    Techniques: Expressing, Plasmid Preparation, Transduction