Journal: Nature Communications

Article Title: κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis

doi: 10.1038/s41467-020-17226-0

Figure Lengend Snippet: a Resected tumours of 10-week-old 1SKO/R and pDKO/R mice (left) and exemplary haematoxylin/eosin staining of a section of 10-week-old pDKO/R mice (right). Scale bar: 500 µm. b Fluorescent IHC staining of CK19 (magenta) and amylase1 (green) in pancreatic sections of 1SKO/R and pDKO/R littermate mice (4 weeks old). Scale bar: 200 µm. c FACS analysis of pancreatic cell populations. Acinar cells were excluded from analysis based on FSC/SSC and residual cells analysed for ductal (MIC-1C3 + ) and acinar cell markers (MPx1 + ) by surface staining. d Fluorescent IHC staining of Cpa1 (magenta, acinar cell marker), Sox9 (green) and nuclei (DAPI, blue) in paraffin sections of 1SKO/R and pDKO/R littermate mice (4 weeks old). Scale bar: 200 µm.

Article Snippet: Primary antibodies and dilutions used: anti-CK19 (1:100, Santa Cruz, sc-33111), anti-Amylase1 (1:100, Cell Signalling, D55H10), anti-Sox9 (1:100, Cell Signalling, D8G8H), anti-Cpa1 (1:100, Novus, AF2765), anti-phospho-p44/42 (1:200, Cell Signalling, Thr202/Tyr204), anti-Ki67 (1:100, Cell Signalling, 12202, clone D3B5) and anti-phospho-Akt substrate (1:100, Cell Signalling 9611).

Techniques: Staining, Immunohistochemistry, Marker

Journal: Nature Communications

Article Title: κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis

doi: 10.1038/s41467-020-17226-0

Figure Lengend Snippet: a Experimental set-up of acute pancreatitis model. b The percentage of pancreatic tissue affected by ADM after cerulein injections was scored in haematoxylin/eosin-stained sections (score 0: 0–2%; score 1: 2–50%; score 2: >50%). Data are presented as mean and single values. Data represent the collected results from two (9- and 24-h time points) or three (48-h and 21-d time points) independent experiments. c , d Sections of pancreata from mice with the indicated genotypes 48 h ( c ) and 21 d ( d ) after cerulein or saline injections were stained with haematoxylin/eosin or antibodies against amylase1 or Sox9. White arrows indicate Sox9 expression in ductal cells. Black arrows indicate Sox9 expression in acinar cells. Scale bar haematoxylin/eosin and amylase: 50 µm. Scale bar: Sox9: 100 µm.

Article Snippet: Primary antibodies and dilutions used: anti-CK19 (1:100, Santa Cruz, sc-33111), anti-Amylase1 (1:100, Cell Signalling, D55H10), anti-Sox9 (1:100, Cell Signalling, D8G8H), anti-Cpa1 (1:100, Novus, AF2765), anti-phospho-p44/42 (1:200, Cell Signalling, Thr202/Tyr204), anti-Ki67 (1:100, Cell Signalling, 12202, clone D3B5) and anti-phospho-Akt substrate (1:100, Cell Signalling 9611).

Techniques: Staining, Expressing