p selectin antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p selectin antibody
    P Selectin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p selectin antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p selectin antibody
    P Selectin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p selectin antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    p selectin antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p selectin antibody
    The C‐terminus of the second extracellular loop vaccine (C‐ EL 2 Vac) inhibits thromboxane A 2 receptor–induced, but not thrombin receptor‐activating peptide 4 (TRAP4)–induced, platelet‐leukocyte aggregate formation. A, Blood from vaccinated mice (ie, C‐ EL 2, random C‐ EL 2 [C‐ EL 2r], or keyhole limpet hemocyanin [ KLH ]) was incubated with <t>anti–P‐selectin</t> (platelet marker) and anti‐ CD 11b (leukocyte marker) antibodies, before incubation with or without U46619 (1 μmol/L). Events double positive for CD 11b and P‐selectin identified platelet‐leukocyte aggregates and were recorded as a percentage of a total of 10 000 gated leukocytes ( KLH vs KLH +U46619, ** P <0.001; C‐ EL 2 Vac vs C‐ EL 2 Vac+U46619, P =not significant [ NS] ; C‐ EL 2r Vac vs C‐ EL 2r Vac+U46619, ** P <0.0015). B, Blood from vaccinated mice (ie, C‐ EL 2, C‐ EL 2r, or KLH ) was incubated with anti–P‐selectin (platelet marker) and anti‐ CD 11b (leukocyte marker) antibodies, before incubation with or without TRAP 4 (80 μmol/L). Events double positive for CD 11b and P‐selectin identified platelet‐leukocyte aggregates and were recorded as a percentage of a total of 10 000 gated leukocytes ( KLH vs KLH + TRAP 4, * P <0.05; C‐ EL 2 Vac vs C‐ EL 2 Vac+ TRAP 4, ** P <0.001; C‐ EL 2r Vac vs C‐ EL 2r Vac+ TRAP 4, ** P <0.001). The error bars in this figure represent SEM . Each experiment was repeated 3 times using 3 separate groups, with blood pooled from 8 mice per group.
    P Selectin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p selectin antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    p selectin antibody - by Bioz Stars, 2023-02
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    1) Product Images from "Investigation of a Thromboxane A 2 Receptor–Based Vaccine for Managing Thrombogenesis"

    Article Title: Investigation of a Thromboxane A 2 Receptor–Based Vaccine for Managing Thrombogenesis

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.118.009139

    The C‐terminus of the second extracellular loop vaccine (C‐ EL 2 Vac) inhibits thromboxane A 2 receptor–induced, but not thrombin receptor‐activating peptide 4 (TRAP4)–induced, platelet‐leukocyte aggregate formation. A, Blood from vaccinated mice (ie, C‐ EL 2, random C‐ EL 2 [C‐ EL 2r], or keyhole limpet hemocyanin [ KLH ]) was incubated with anti–P‐selectin (platelet marker) and anti‐ CD 11b (leukocyte marker) antibodies, before incubation with or without U46619 (1 μmol/L). Events double positive for CD 11b and P‐selectin identified platelet‐leukocyte aggregates and were recorded as a percentage of a total of 10 000 gated leukocytes ( KLH vs KLH +U46619, ** P <0.001; C‐ EL 2 Vac vs C‐ EL 2 Vac+U46619, P =not significant [ NS] ; C‐ EL 2r Vac vs C‐ EL 2r Vac+U46619, ** P <0.0015). B, Blood from vaccinated mice (ie, C‐ EL 2, C‐ EL 2r, or KLH ) was incubated with anti–P‐selectin (platelet marker) and anti‐ CD 11b (leukocyte marker) antibodies, before incubation with or without TRAP 4 (80 μmol/L). Events double positive for CD 11b and P‐selectin identified platelet‐leukocyte aggregates and were recorded as a percentage of a total of 10 000 gated leukocytes ( KLH vs KLH + TRAP 4, * P <0.05; C‐ EL 2 Vac vs C‐ EL 2 Vac+ TRAP 4, ** P <0.001; C‐ EL 2r Vac vs C‐ EL 2r Vac+ TRAP 4, ** P <0.001). The error bars in this figure represent SEM . Each experiment was repeated 3 times using 3 separate groups, with blood pooled from 8 mice per group.
    Figure Legend Snippet: The C‐terminus of the second extracellular loop vaccine (C‐ EL 2 Vac) inhibits thromboxane A 2 receptor–induced, but not thrombin receptor‐activating peptide 4 (TRAP4)–induced, platelet‐leukocyte aggregate formation. A, Blood from vaccinated mice (ie, C‐ EL 2, random C‐ EL 2 [C‐ EL 2r], or keyhole limpet hemocyanin [ KLH ]) was incubated with anti–P‐selectin (platelet marker) and anti‐ CD 11b (leukocyte marker) antibodies, before incubation with or without U46619 (1 μmol/L). Events double positive for CD 11b and P‐selectin identified platelet‐leukocyte aggregates and were recorded as a percentage of a total of 10 000 gated leukocytes ( KLH vs KLH +U46619, ** P <0.001; C‐ EL 2 Vac vs C‐ EL 2 Vac+U46619, P =not significant [ NS] ; C‐ EL 2r Vac vs C‐ EL 2r Vac+U46619, ** P <0.0015). B, Blood from vaccinated mice (ie, C‐ EL 2, C‐ EL 2r, or KLH ) was incubated with anti–P‐selectin (platelet marker) and anti‐ CD 11b (leukocyte marker) antibodies, before incubation with or without TRAP 4 (80 μmol/L). Events double positive for CD 11b and P‐selectin identified platelet‐leukocyte aggregates and were recorded as a percentage of a total of 10 000 gated leukocytes ( KLH vs KLH + TRAP 4, * P <0.05; C‐ EL 2 Vac vs C‐ EL 2 Vac+ TRAP 4, ** P <0.001; C‐ EL 2r Vac vs C‐ EL 2r Vac+ TRAP 4, ** P <0.001). The error bars in this figure represent SEM . Each experiment was repeated 3 times using 3 separate groups, with blood pooled from 8 mice per group.

    Techniques Used: Incubation, Marker

    mouse monoclonal o4 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal o4 antibody
    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of <t>O4</t> <t>(green),</t> <t>Olig2</t> (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    Mouse Monoclonal O4 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal o4 antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
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    mouse monoclonal o4 antibody - by Bioz Stars, 2023-02
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    1) Product Images from "FGF signaling controls Shh-dependent oligodendroglial fate specification in the ventral spinal cord"

    Article Title: FGF signaling controls Shh-dependent oligodendroglial fate specification in the ventral spinal cord

    Journal: Neural Development

    doi: 10.1186/s13064-018-0100-2

    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of O4 (green), Olig2 (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    Figure Legend Snippet: Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of O4 (green), Olig2 (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g" , h'' ) and Nkx2.2 (blue in g , h , g' and h' , g''' , h''' ) after electroporation of control (green in g ) or dnFGFR (green in h ) vectors. Note reduction of the dorsal extent of the Nkx2.2 positive domain on the side of explant electroporated with the dnFGFR vector compared to the non electroporated side of the explant or with explant electroporated with the control vector (brackets in g' and h' ). Note also that migrating Olig2/Nkx2.2 double-labeled cells are not detected on the side of explant electroporated with the dnFGFR vector. i Quantification of Olig2-positive cells in the mantle zone in control conditions ( n = 7) or in presence of the FGFR inhibitor SU5402 ( n = 8). j Quantification of Nkx2.2/Olig2-positive cells in the progenitor zone (PZ) of explants cultivated in control conditions ( n = 7) or in presence of SU5402 ( n = 8). k - l Quantification of Olig2-positive cells migrating in the mantle zone on each side of the explants after electroporation of control ( n = 4, k ) or dnFGFR ( n = 9, l ) vectors. Results are presented as mean number of cells ± sem (** p ≤ 0.01; *** p ≤ 0.001). ne: non-electroporated side of explants. PZ = progenitor zone, MZ = mantle zone, MFP = medial floor plate, V = ventral, D = dorsal. Scale bars = 50 μm in b - d , g , h and 25 μm in e , f

    Techniques Used: Immunodetection, Electroporation, Plasmid Preparation, Labeling

    anti p selectin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p selectin
    Anti P Selectin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e selectin monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc e selectin monoclonal antibody
    E Selectin Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    r 14  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc r 14
    R 14, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal antibody
    Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc p selectin antibody
    P Selectin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mouse monoclonal o4 antibody
    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of <t>O4</t> <t>(green),</t> <t>Olig2</t> (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    Mouse Monoclonal O4 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal o4 antibody/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti p selectin
    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of <t>O4</t> <t>(green),</t> <t>Olig2</t> (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    Anti P Selectin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc e selectin monoclonal antibody
    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of <t>O4</t> <t>(green),</t> <t>Olig2</t> (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    E Selectin Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc r 14
    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of <t>O4</t> <t>(green),</t> <t>Olig2</t> (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    R 14, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r 14/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc mouse monoclonal antibody
    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of <t>O4</t> <t>(green),</t> <t>Olig2</t> (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g
    Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of O4 (green), Olig2 (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g

    Journal: Neural Development

    Article Title: FGF signaling controls Shh-dependent oligodendroglial fate specification in the ventral spinal cord

    doi: 10.1186/s13064-018-0100-2

    Figure Lengend Snippet: Inactivation of FGFRs impairs OPC specification. a Scheme of a transverse section through spinal cord explant cultivated as opened-book showing ventral progenitor domains. The dashed blue panels outline the areas shown in images below. b Immunodetection of O4 (green), Olig2 (red) and Nkx2.2 (blue) on transverse section of spinal cord explant cultivated for 2 days in control condition. Note overlapping of the three markers both in the p* domain and in cells emigrating ventrally in the mantle zone. c - d Immunodetection of Olig2 (red in c and d , c' , d' ) and Nkx2.2 (green in c and d , c'' , d'' ) on transverse sections of spinal cord explants cultivated for 2 days in control condition ( c ) or in presence of SU5402 ( d ). Note that while Nkx2.2/Olig2-positive OPCs have already invaded the mantle zone in control explants, very few develop in presence of the inhibitor. Note also reduced dorsal extent of the Nkx2.2-positive domain (brackets) in SU5402 treated explant ( d” ) compared to control explant ( c″ ). e - f Higher magnification of the progenitor zone showing reduced number of Olig2/Nkx2.2-positive cells in the progenitor zone of explant treated with SU5402 ( f , f' , f'' ) compared to control explant ( e , e' , e'' ). g-h Immunodetection of Olig2 (red in g , g' , h and h' , g" , h'' ) and Nkx2.2 (blue in g , h , g' and h' , g''' , h''' ) after electroporation of control (green in g ) or dnFGFR (green in h ) vectors. Note reduction of the dorsal extent of the Nkx2.2 positive domain on the side of explant electroporated with the dnFGFR vector compared to the non electroporated side of the explant or with explant electroporated with the control vector (brackets in g' and h' ). Note also that migrating Olig2/Nkx2.2 double-labeled cells are not detected on the side of explant electroporated with the dnFGFR vector. i Quantification of Olig2-positive cells in the mantle zone in control conditions ( n = 7) or in presence of the FGFR inhibitor SU5402 ( n = 8). j Quantification of Nkx2.2/Olig2-positive cells in the progenitor zone (PZ) of explants cultivated in control conditions ( n = 7) or in presence of SU5402 ( n = 8). k - l Quantification of Olig2-positive cells migrating in the mantle zone on each side of the explants after electroporation of control ( n = 4, k ) or dnFGFR ( n = 9, l ) vectors. Results are presented as mean number of cells ± sem (** p ≤ 0.01; *** p ≤ 0.001). ne: non-electroporated side of explants. PZ = progenitor zone, MZ = mantle zone, MFP = medial floor plate, V = ventral, D = dorsal. Scale bars = 50 μm in b - d , g , h and 25 μm in e , f

    Article Snippet: Secondary antibodies were applied at room temperature for 1 h. The antibodies used were as follows: rabbit anti Phospho-p44/42 MAPK (PERK) 1/200 (Cell Signaling); rabbit anti-Olig2 1/500 (Chemicon); mouse monoclonal O4 antibody (O4 mAb), 1/4 (culture supernatant obtained from O4 hybridoma cells, a gift from R. Bansal); mouse anti-Nkx2.2 1/4 (DSHN) [ ]; rabbit anti-clived Caspase 3 1/400 (Cell Signaling), mouse anti-BrdU 1/2000 (DHSB).

    Techniques: Immunodetection, Electroporation, Plasmid Preparation, Labeling